%A Li,Man
%A Liu,Zhi-Hua
%A Hao,Naijia
%A Olson,Michelle L.
%A Li,Qiang
%A Bhagia,Samarthya
%A Shinde,Somnath
%A Kao,Katy C.
%A Ragauskas,Arthur J.
%A Xie,Shangxian
%A Yuan,Joshua S.
%D 2021
%J Frontiers in Energy Research
%C
%F
%G English
%K lignocellulosic biomass,Lipid,Organosolv,Fenton,Formic acid,pretreatment,Biomimicking Processing
%Q
%R 10.3389/fenrg.2020.00194
%W
%L
%M
%P
%7
%8 2021-February-04
%9 Brief Research Report
%#
%! Organosolv Biomimicking Biomass Processing
%*
%<
%T Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing
%U https://www.frontiersin.org/articles/10.3389/fenrg.2020.00194
%V 8
%0 JOURNAL ARTICLE
%@ 2296-598X
%X The sustainability and economic feasibility of modern biorefinery depend on the efficient processing of both carbohydrate and lignin fractions for value-added products. By mimicking the biomass degradation process in white-rote fungi, a tailored two-step fractionation process was developed to maximize the sugar release from switchgrass biomass and to optimize the lignin processability for bioconversion. Biomimicking biomass processing using Formic Acid: Fenton: Organosolv (F2O) and achieved high processability for both carbohydrate and lignin. Specifically, switchgrass pretreated by the F2O process had 99.6% of the theoretical yield for glucose release. The fractionated lignin was also readily processable by fermentation via Rhodococcus opacus PD630 with a lipid yield of 1.16 g/L. Scanning electron microscope analysis confirmed the fragmentation of switchgrass fiber and the cell wall deconstruction by the F2O process. 2D-HSQC NMR further revealed the cleavage of aryl ether linkages (β-O-4) in lignin components. These results revealed the mechanisms for efficient sugar release and lignin bioconversion. The F2O process demonstrated effective mimicking of natural biomass utilization system and paved a new path for improving the lignin and carbohydrate processability in next generation lignocellulosic biorefinery.