AUTHOR=Cummings Charles O., Hill Nichola J., Puryear Wendy B., Rogers Benjamin, Mukherjee Jean, Leibler Jessica H., Rosenbaum Marieke H., Runstadler Jonathan A. TITLE=Evidence of Influenza A in Wild Norway Rats (Rattus norvegicus) in Boston, Massachusetts JOURNAL=Frontiers in Ecology and Evolution VOLUME=7 YEAR=2019 URL=https://www.frontiersin.org/articles/10.3389/fevo.2019.00036 DOI=10.3389/fevo.2019.00036 ISSN=2296-701X ABSTRACT=Influenza A virus (IAV) is known to circulate among human and animal reservoirs, yet there are few studies that address the potential for urban rodents to carry and shed IAV. Rodents are often used as influenza models in the lab, but the few field studies that have looked for evidence of IAV in rodents have done so primarily in rural areas following outbreaks of IAV in poultry. This study sought to assess the prevalence of IAV recovered from wild Norway rats in a dense urban location (Boston). To do this, we sampled the oronasal cavity, paws, and lungs of Norway rats trapped by the City of Boston's Inspectional Services from December 2016 to September 2018. All samples were screened by real-time, reverse transcriptase PCR targeting the conserved IAV matrix segment. A total of 163 rats were trapped, 18 of which (11.04%) were RT-PCR positive for IAV in either oronasal swabs (9), paw swabs (9), both (2), or lung homogenates (2). A generalized linear model indicated that month and geographic location were correlated with IAV-positive PCR status of rats. A seasonal trend in IAV-PCR status was observed with the highest prevalence occurring in the winter months (December–January) followed by a decline over the course of the year, reaching its lowest prevalence in September. Sex and weight of rats were not significantly associated with IAV-PCR status, suggesting that rodent demography is not a primary driver of infection. This pilot study provides evidence of the need to further investigate the role that wild rats may play as reservoirs or mechanical vectors for IAV circulation in urban environments across seasons.