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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2018.00652

A major and stable QTL for bacterial wilt resistance on chromosome B02 identified using a high-density SNP-based genetic linkage map in cultivated peanut Yuanza 9102

 Lifang Wang1, Xiaojing Zhou1, Xiaoping Ren1, Li Huang1, Huanyong Luo1, Yuning Chen1, Weigang Chen1, Nian Liu1,  Boshou Liao1,  Yong Lei1, Liying Yan1,  Jinxiong Shen2 and  Huifang Jiang1*
  • 1Oil Crops Research Institute (CAAS), China
  • 2Huazhong Agricultural University, China

Bacterial wilt (BW) is one of important diseases limiting the production of peanut (Arachis hypogaea L.) worldwide. The sufficient precise information on the quantitative trait loci (QTLs) for BW resistance is essential for facilitating gene mining and applying in molecular breeding. Cultivar Yuanza 9102 is BW resistant, bred from wide cross between cultivated peanut Baisha 1016 and a wild diploid peanut species A.chacoense with BW resistance. In this study, we aim to map the major QTLs related to BW-resistance in Yuanza 9102. A high density SNP-based genetic linkage map was constructed through double-digest restriction-site-associated DNA sequencing (ddRADseq) technique based on Yuanza 9102-derived recombinant inbred lines population. The map contained 2,187 SNP markers distributed on 20 linkage groups (LGs) spanning 1566.10 cM, and showed good synteny with A genome from A.duranensis and B genome from A.ipaensis. Phenotypic frequencies of BW resistance among RIL population showed obvious bi-model distribution in four environments. Four QTLs explaining 5.49% to 23.22% phenotypic variance were identified to be all located on chromosome B02. The major QTL, qBWB02.1 (12.17-23.33% PVE), was detected in three environments showing consistent and stable expression. Furthermore, there was positive additive effect among these major and minor QTLs. The major QTL region was identified on a segment covering 2.3Mb of the pseudomolecule B02 of A. ipaensis which resides in cluster of 21 NB-LRR encoding genes. The results from the present study provides further investigation for gene discovery and marker assistant breeding in peanut.

Keywords: Peanut cultivars, Bacterial wilt resistance, ddRADSeq, Genetic map, QTLs

Received: 06 Aug 2018; Accepted: 30 Nov 2018.

Edited by:

Genlou Sun, Saint Mary's University, Canada

Reviewed by:

Milind Ratnaparkhe, ICAR Indian Institute of Soybean Research, India
Bangquan Huang, Hubei University, China
Huilan CHEN, Huazhong Agricultural University, China
Mei Yuan, Shandong Peanut Research institute  

Copyright: © 2018 Wang, Zhou, Ren, Huang, Luo, Chen, Chen, Liu, Liao, Lei, Yan, Shen and Jiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Huifang Jiang, Oil Crops Research Institute (CAAS), Wuhan, China, peanutlab@oilcrops.cn