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This article is part of the Research Topic

Single Cell Genomics: Technology and Application

Perspective ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.00008

The impact of heterogeneity on single-cell sequencing

 Samantha L. Goldman1, Matthew J. MacKay1,  Ebrahim Afshinnekoo1,  Ari Melnick2, Shixiu Wu3 and  Christopher E. Mason1, 4, 5*
  • 1Weill Cornell Medicine, Cornell University, United States
  • 2Department of Medicine, Weill Cornell Medicine, Cornell University, United States
  • 3Hangzhou Cancer Hospital, China
  • 4Feil Family Brain & Mind Research Institute, Weill Cornell Medicine, United States
  • 5Institute for Computational Biomedicine, Weill Cornell Medicine, Cornell University, United States

The importance of diversity and specialization is clear for many reasons, from population-level diversification to improve resiliency to unforeseen stresses to specialization within multicellular organisms from development and differentiation. However, the level of cellular heterogeneity is just now becoming clear through the integration of genome-wide analyses such as Next Generation Sequencing. With the rise of single-cell NGS (scNGS) we are now capable of witnessing how diverse cells are at different levels of gene expression and somatic mutational heterogeneity. In this perspective we discuss the importance of understanding heterogeneity and the application of NGS, with a focus on the utilization of single cell next generation sequencing and a guide to aid in sample size and experimentation design.

Keywords: scRNA-seq, NGS, RNA, single cells, heterogeneity, RNA, heterogeneity, single cell, ScRNA

Received: 20 Jul 2018; Accepted: 09 Jan 2019.

Edited by:

Xinghua V. Pan, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Southern Medical University, China

Reviewed by:

Saheli Sarkar, Northeastern University, United States
Guangshuai Jia, Max Planck Institute for Heart and Lung Research, Germany  

Copyright: © 2019 Goldman, MacKay, Afshinnekoo, Melnick, Wu and Mason. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Christopher E. Mason, Weill Cornell Medicine, Cornell University, White Plains, United States, chm2042@med.cornell.edu