%A Felkel,Sabine %A Wallner,Barbara %A Chuluunbat,Battsesteg %A Yadamsuren,Adiya %A Faye,Bernard %A Brem,Gottfried %A Walzer,Chris %A Burger,Pamela A. %D 2019 %J Frontiers in Genetics %C %F %G English %K Old World camelids,Paternal lineage,Y Chromosome,genotyping,diversity,Conservation -,ancestry %Q %R 10.3389/fgene.2019.00423 %W %L %M %P %7 %8 2019-May-21 %9 Original Research %# %! A prime Y-phylogeny in Bactrian camels %* %< %T A First Y-Chromosomal Haplotype Network to Investigate Male-Driven Population Dynamics in Domestic and Wild Bactrian Camels %U https://www.frontiersin.org/articles/10.3389/fgene.2019.00423 %V 10 %0 JOURNAL ARTICLE %@ 1664-8021 %X Polymorphic markers on the male-specific part of the Y chromosome (MSY) provide useful information for tracking male genealogies. While maternal lineages are well studied in Old World camelids using mitochondrial DNA, the lack of a Y-chromosomal reference sequence hampers the analysis of male-driven demographics. Recently, a shotgun assembly of the horse MSY was generated based on short read next generation sequencing data. The haplotype network resulting from single copy MSY variants using the assembly as a reference revealed sufficient resolution to trace individual male lines in this species. In a similar approach we generated a 3.8 Mbp sized assembly of the MSY of Camelus bactrianus. The camel MSY assembly was used as a reference for variant calling using short read data from eight Old World camelid individuals. Based on 596 single nucleotide variants we revealed a Y-phylogenetic network with seven haplotypes. Wild and domestic Bactrian camels were clearly separated into two different haplogroups with an estimated divergence time of 26,999 ± 2,268 years. Unexpectedly, one wild camel clustered into the domestic Bactrian camels’ haplogroup. The observation of a domestic paternal lineage within the wild camel population is concerning in view of the importance to conserve the genetic integrity of these highly endangered species in their natural habitat.