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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.00492

Comparative genomic analysis reveals extensive genetic and functional variations of WRKYs in solanaceae

 Yuan Cheng1,  Golam Jalal Ahammed2, zhuping yao1, qingjing ye1, meiying ruan1, rongqing wang1,  Zhimiao Li1, guozhi zhou1* and  Hongjian Wan1*
  • 1ZheJiang Academy of Agricultural Sciences, China
  • 2Henan University of Science and Technology, China

As a conserved protein family, WRKY has been shown to be involved in multiple biological processes in plants. However, the mechanism of functional diversity for WRKYs has not been well elucidated. Here, totally 223 solanaceae WRKY members from pepper, tomato and potato, were analyzed using comparative genomics. A tremendous genetic variation among WRKY members of different solanaceous plants or groups was demonstrated by the variations of WRKY number/size, group constitution, phylogenetic relationship, gene structure, and domain composition. Except for the extensive genetic variations, certain degree of conservatism for solanaceae WRKYs was also revealed. The phylogenetic analysis showed that except for the known WRKY groups (I, IIa/b/c/d/e and III), two extra WRKY subgroups specifically existed in solanaceous plants, which were named as group IIf and group IIg for the convenience of group-wise analysis in this study. Moreover, the variant zinc-finger structure (CX4,7CX22-24HXC) in group III of solanaceae WRKYs was identified. Expression profile of CaWRKY genes suggested their potential roles in pepper development and stress responses, and demonstrated a functional division pattern for pepper CaWRKYs. Furthermore, functional analysis using Virus Induced Gene Silencing (VIGS) revealed critical roles of two CaWRKYs (CaWRKY45 and CaWRKY58) in plant response to disease and drought, respectively. This study provides a solid foundation for further dissection of the evolutionary and functional diversity of solanaceae WRKYs in crop plants.

Keywords: WRKY, Solanaceae, Genetic Variation, Genetic conservatism, functional diversity

Received: 20 Jan 2019; Accepted: 06 May 2019.

Edited by:

Genlou Sun, Saint Mary's University, Canada

Reviewed by:

Bayram Cevik, Isparta University of Applied Sciences, Turkey
Jingyin Yu, University of Georgia, United States  

Copyright: © 2019 Cheng, Ahammed, yao, ye, ruan, wang, Li, zhou and Wan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. guozhi zhou, ZheJiang Academy of Agricultural Sciences, Hangzhou, 310000, Zhejiang, China, Chinazhougz@163.com
Prof. Hongjian Wan, ZheJiang Academy of Agricultural Sciences, Hangzhou, 310000, Zhejiang, China, wanhongjian@sina.com