AUTHOR=Zhang Wenjing , Li Min , Sun Feng , Xu Xuting , Zhang Zhaofeng , Liu Junwei , Sun Xiaowei , Zhang Aiping , Shen Yupei , Xu Jianhua , Miao Maohua , Wu Bin , Yuan Yao , Huang Xianliang , Shi Huijuan , Du Jing 

TITLE=Association of Sperm Methylation at LINE-1, Four Candidate Genes, and Nicotine/Alcohol Exposure With the Risk of Infertility

JOURNAL=Frontiers in Genetics

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2019.01001

DOI=10.3389/fgene.2019.01001

ISSN=1664-8021

ABSTRACT=<p>In this study, we examined whether smoking and drinking affect sperm quality and the DNA methylation of the repetitive element <italic>LINE-1</italic>, <italic>MEST</italic>, <italic>P16</italic>, <italic>H19</italic>, and <italic>GNAS</italic> in sperm. Semen samples were obtained from 143 male residents in a minority-inhabited district of Guizhou province in southwest China. Quantitative DNA methylation analysis of the samples was performed using MassARRAY EpiTYPER assays. Sperm motility was significantly lower in both the nicotine-exposed (P = 0.0064) and the nicotine- and alcohol-exposed (P = 0.0008) groups. Follicle-stimulating hormone (FSH) levels were higher in the nicotine-exposed group (P = 0.0026). The repetitive element <italic>LINE-1</italic> was hypermethylated in the three exposed groups, while <italic>P16</italic> was hypomethylated in the alcohol and both the alcohol and nicotine exposure groups. Our results also show that alcohol and nicotine exposure altered sperm cell quality, which may be related to the methylation levels of <italic>MEST</italic> and <italic>GNAS</italic>. In addition, <italic>MEST</italic>, <italic>GNAS</italic>, and the repetitive element <italic>LINE1</italic> methylation was significantly associated with the concentration of sperm as well as FSH and luteinizing hormone levels.</p>