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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Genet. | doi: 10.3389/fgene.2019.01187

Identification of Microenvironment-related Prognostic Genes in Bladder Cancer Based on Gene Expression Profile

 Yongxiang Luo1, Guohua Zeng2 and Song Wu1*
  • 1Shenzhen Luohu People's Hospital, China
  • 2First Affiliated Hospital of Guangzhou Medical University, China

Background and Objective: Bladder cancer is the most common tumor in the urinary system, with a higher incidence in men than in women and a high recurrence rate. However, the mechanism of recurrence is still unclear, and it is urgent to clarify the pathophysiological mechanism of bladder cancer. To provide theoretical basis for the development of new therapies, investigating the effect of tumor microenvironment on the prognosis of bladder cancer is necessary.
Methods: We applied the ESTIMATE algorithm to the downloaded TCGA transcriptome data to obtain the immune scores and stromal scores of each sample, and then divided the samples into two groups: high and low immune scores (or high and low stromal scores), and found that some differential genes were associated with poor prognosis of patients. We then performed PPI network analysis to explore the relationship between these differentially expressed genes. Moreover, we also performed GO and KEGG analyses to explore the potential functions of differentially expressed genes. Finally, our results were validated in an independent dataset.
Results: We identified 136 tumor microenvironment-related genes associated with poor prognosis of bladder cancer. GO annotation and KEGG pathway enrichment analysis found that these genes are mainly involved in extracellular matrix, Focal adhesion and PI3k-Akt signaling pathway. Next, PPI network analysis revealed some hub genes including VCAN, THBS1 and THBS2. Finally, 27 genes were further verified in the independent data set.
Conclusions: We found 27 tumor microenvironment-related genes of bladder cancer, which are associated with poor prognosis of bladder cancer. These genes may inspire researchers to develop new treatments for bladder cancer.

Keywords: Bladder cancer, gene expression profile, Microenvironment,, Immune scores, Stromal scores

Received: 18 Jul 2019; Accepted: 25 Oct 2019.

Copyright: © 2019 Luo, Zeng and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Song Wu, Shenzhen Luohu People's Hospital, Shenzhen, China, wusong@szu.edu.cn