Original Research ARTICLE
Molecular and Phylogenetic Analyses of the Mediator Subunit Genes in Solanum lycopersicum
- 1Chongqing University, China
The Mediator complex is a multi-subunit protein assembly that serves as a central scaffold to help regulate DNA-binding transcription factors (TFs) and RNA polymerase II (Pol II) activity controlled gene expression programmed in response to developmental or environmental factors. However，litter information about Mediator complex subunit (MED) genes in tomato is available, although it is an essential model plant. In this study, we retrieved 46 candidate SlMED genes from the genome of tomato, and a comprehensive analysis was conducted, including their phylogenetic relationship, chromosomal locations, gene structure, cis-regulatory elements prediction as well as gene expression. The expression profiling of 46 SlMED genes was analyzed using publicly available RNA-seq data. Furthermore, we selected some SlMED genes to evaluate their expression patterns in various tissues and under different abiotic stress treatments by qRT-PCR experiments. This is the first detailed report to elucidate the molecular and phylogenetic features of the MED genes in tomato, and it provides valuable clues for further functional analysis in order to clarify the role of the SlMED genes in diverse plant growth, development and abiotic stress response.
Keywords: Mediator complex subunits (MED), Solanum lycopersicum, Genomic characterization, expression analysis, Plant stress response
Received: 03 Sep 2019;
Accepted: 05 Nov 2019.
Copyright: © 2019 Wang, Liang, Chen, Liao, Wang, Hu and Xie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Prof. Zongli Hu, Chongqing University, Chongqing, China, email@example.com
Prof. Qiaoli Xie, Chongqing University, Chongqing, China, firstname.lastname@example.org