AUTHOR=Qiu Yinjie , Hirsch Cory D. , Yang Ya , Watkins Eric 

TITLE=Towards Improved Molecular Identification Tools in Fine Fescue (Festuca L., Poaceae) Turfgrasses: Nuclear Genome Size, Ploidy, and Chloroplast Genome Sequencing

JOURNAL=Frontiers in Genetics

VOLUME=Volume 10 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2019.01223

DOI=10.3389/fgene.2019.01223

ISSN=1664-8021

ABSTRACT=Fine fescues (Festuca L., Poaceae) are turfgrass species that perform well in low-input environments. Based on morphological characteristics, the most commonly-utilized fine fescues are divided into five taxa: three are subspecies within F. rubra L. and the remaining two are treated as species within the F. ovina L. complex. Morphologically, these five taxa are very similar, and both identification and classification of fine fescues remain challenging. In an effort to develop classification methods for fescues, we used flow cytometry to estimate genome size and sequenced the chloroplast genome of all five taxa. Fine fescue chloroplast genome sizes ranged from 133,331 to 133,841 bp and contained 113-114 genes. Phylogenetic reconstruction using whole plastid genome sequences agreed with previous work based on morphology. Comparative genomics suggested unique repeat signatures for each fine fescue taxon and could potentially be used for marker development for taxon identification.