TY - JOUR AU - Pepin, Mark E. AU - Infante, Teresa AU - Benincasa, Giuditta AU - Schiano, Concetta AU - Miceli, Marco AU - Ceccarelli, Simona AU - Megiorni, Francesca AU - Anastasiadou, Eleni AU - Della Valle, Giovanni AU - Fatone, Gerardo AU - Faenza, Mario AU - Docimo, Ludovico AU - Nicoletti, Giovanni F. AU - Marchese, Cinzia AU - Wende, Adam R. AU - Napoli, Claudio PY - 2020 M3 - Original Research TI - Differential DNA Methylation Encodes Proliferation and Senescence Programs in Human Adipose-Derived Mesenchymal Stem Cells JO - Frontiers in Genetics UR - https://www.frontiersin.org/articles/10.3389/fgene.2020.00346 VL - 11 SN - 1664-8021 N2 - Adult adipose tissue-derived mesenchymal stem cells (ASCs) constitute a vital population of multipotent cells capable of differentiating into numerous end-organ phenotypes. However, scientific and translational endeavors to harness the regenerative potential of ASCs are currently limited by an incomplete understanding of the mechanisms that determine cell-lineage commitment and stemness. In the current study, we used reduced representation bisulfite sequencing (RRBS) analysis to identify epigenetic gene targets and cellular processes that are responsive to 5′-azacitidine (5′-AZA). We describe specific changes to DNA methylation of ASCs, uncovering pathways likely associated with the enhancement of their proliferative capacity. We identified 4,797 differentially methylated regions (FDR < 0.05) associated with 3,625 genes, of which 1,584 DMRs annotated to the promoter region. Gene set enrichment of differentially methylated promoters identified “phagocytosis,” “type 2 diabetes,” and “metabolic pathways” as disproportionately hypomethylated, whereas “adipocyte differentiation” was the most-enriched pathway among hyper-methylated gene promoters. Weighted coexpression network analysis of DMRs identified clusters associated with cellular proliferation and other developmental programs. Furthermore, the ELK4 binding site was disproportionately hyper-methylated within the promoters of genes associated with AKT signaling. Overall, this study offers numerous preliminary insights into the epigenetic landscape that influences the regenerative capacity of human ASCs. ER -