AUTHOR=Li Ronghua, Zhang Weijia, Lu Junkai, Zhang Zhouyi, Mu Changkao, Song Weiwei, Migaud Herve, Wang Chunlin, Bekaert Michaƫl TITLE=The Whole-Genome Sequencing and Hybrid Assembly of Mytilus coruscus JOURNAL=Frontiers in Genetics VOLUME=11 YEAR=2020 URL=https://www.frontiersin.org/articles/10.3389/fgene.2020.00440 DOI=10.3389/fgene.2020.00440 ISSN=1664-8021 ABSTRACT=The hard-shelled mussel (Mytilus coruscus) is an economically important shellfish that has been cultivated for the last decade. Due to over-exploitation, most mussel stocks have dramatically declined. Efforts to study this species' natural distribution, genetics, breeding, and cultivation have been hindered by the lack of a high-quality reference genome. To address this, we produced a hybrid high-quality reference genome of M. coruscus using a long-read platform to assemble the genome and short-read, high-quality technology to accurately correct for sequence errors. The genome was assembled into 10,484 scaffolds, a total length of 1.90 Gb, and a scaffold N50 of 898 kb. Ab initio annotation of the M. coruscus genome assembly identified a total of 42,684 genes. This accurate reference genome of M. coruscus provides an essential resource with the advantage of enabling the genome-scale selective breeding of M. coruscus. More importantly, it will also help in deciphering the speciation and local adaptation of the Mytilus species.