%A Xing,Qiao Rui %A Cipta,Nadia Omega %A Hamashima,Kiyofumi %A Liou,Yih-Cherng %A Koh,Cheng Gee %A Loh,Yuin-Han %D 2020 %J Frontiers in Genetics %C %F %G English %K Multimodal Single-cell Techniques,Spatial transcriptome,Transcriptome,chromatin accessibility,integrative analysis %Q %R 10.3389/fgene.2020.00662 %W %L %M %P %7 %8 2020-July-17 %9 Review %# %! Single-cell multi-omics %* %< %T Unraveling Heterogeneity in Transcriptome and Its Regulation Through Single-Cell Multi-Omics Technologies %U https://www.frontiersin.org/articles/10.3389/fgene.2020.00662 %V 11 %0 JOURNAL ARTICLE %@ 1664-8021 %X Cellular heterogeneity plays a pivotal role in tissue homeostasis and the disease development of multicellular organisms. To deconstruct the heterogeneity, a multitude of single-cell toolkits measuring various cellular contents, including genome, transcriptome, epigenome, and proteome, have been developed. More recently, multi-omics single-cell techniques enable the capture of molecular footprints with a higher resolution by simultaneously profiling various cellular contents within an individual cell. Integrative analysis of multi-omics datasets unravels the relationships between cellular modalities, builds sophisticated regulatory networks, and provides a holistic view of the cell state. In this review, we summarize the major developments in the single-cell field and review the current state-of-the-art single-cell multi-omic techniques and the bioinformatic tools for integrative analysis.