AUTHOR=Govindaraj Chindu , Scalzo-Inguanti Karen , Scholzen Anja , Li Shuo , Plebanski Magdalena TITLE=TNFR2 Expression on CD25hiFOXP3+ T Cells Induced upon TCR Stimulation of CD4 T Cells Identifies Maximal Cytokine-Producing Effectors JOURNAL=Frontiers in Immunology VOLUME=4 YEAR=2013 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2013.00233 DOI=10.3389/fimmu.2013.00233 ISSN=1664-3224 ABSTRACT=

In this study, we show that CD25^hiTNFR2⁺ cells can be rapidly generated in vitro from circulating CD4 lymphocytes by polyclonal stimuli anti-CD3 in the presence of anti-CD28. The in vitro induced CD25^hiTNFR2⁺ T cells express a conventional regulatory T cells phenotype FOXP3⁺CTLA4⁺CD127^lo/−, but produce effector and immunoregulatory cytokines including IL-2, IL-10, and IFN-g. These induced CD25^hiTNFR2⁺ T cells do not suppress target cell proliferation, but enhance it instead. Thus the CD25^hiTNFR2⁺ phenotype induced rapidly following CD3/28 cross linking of CD4 T cells identifies cells with maximal proliferative and effector cytokine-producing capability. The in vivo counterpart of this cell population may play an important role in immune response initiation.