AUTHOR=Budding Kevin , van de Graaf Eduard A. , Kardol-Hoefnagel Tineke , Oudijk Erik-Jan D. , Kwakkel-van Erp Johanna M. , Hack C. Erik , Otten Henny G. TITLE=Antibodies against Apoptotic Cells Present in End-stage Lung Disease Patients Do Not Correlate with Clinical Outcome after Lung Transplantation JOURNAL=Frontiers in Immunology VOLUME=8 YEAR=2017 URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2017.00322 DOI=10.3389/fimmu.2017.00322 ISSN=1664-3224 ABSTRACT=
Antibodies against HLA and non-HLA are associated with transplantation outcome. Recently, pretransplant serum IgG antibody levels against apoptotic cells were found to correlate with kidney allograft loss. We investigated the presence of these antibodies in lung transplantation (LTx) patients and evaluated the correlation of pre-LTx serum levels of IgG antibodies against apoptotic cells with LTx outcome. These cells included donor lung endothelial cells (ECs) obtained from lung perfusion fluid collected during LTx procedure. Cells were isolated, expanded