%A Vacca,Maurizio
%A Böhme,Julia
%A Zambetti,Lia Paola
%A Khameneh,Hanif Javanmard
%A Paleja,Bhairav S.
%A Laudisi,Federica
%A Ho,Adrian W. S.
%A Neo,Kurt
%A Leong,Keith Weng Kit
%A Marzuki,Mardiana
%A Lee,Bernett
%A Poidinger,Michael
%A Santambrogio,Laura
%A Tsenova,Liana
%A Zolezzi,Francesca
%A De Libero,Gennaro
%A Singhal,Amit
%A Mortellaro,Alessandra
%D 2017
%J Frontiers in Immunology
%C
%F
%G English
%K NLRP10,Dendritic Cells,CpG DNA,Toll-Like Receptor 9,il-12,T helper 1,IFNg,Mycobacterium tuberculosis
%Q
%R 10.3389/fimmu.2017.01462
%W
%L
%M
%P
%7
%8 2017-November-02
%9 Original Research
%+ Alessandra Mortellaro,Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR),Singapore,alessandra_mortellaro@immunol.a-star.edu.sg
%+ Alessandra Mortellaro,Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore,Singapore,alessandra_mortellaro@immunol.a-star.edu.sg
%#
%! NLRP10 enhances the Th1 immune response
%*
%<
%T NLRP10 Enhances CD4+ T-Cell-Mediated IFNγ Response via Regulation of Dendritic Cell-Derived IL-12 Release
%U https://www.frontiersin.org/articles/10.3389/fimmu.2017.01462
%V 8
%0 JOURNAL ARTICLE
%@ 1664-3224
%X NLRP10 is a nucleotide-binding oligomerization domain-like receptor that functions as an intracellular pattern recognition receptor for microbial products. Here, we generated a Nlrp10−/− mouse to delineate the role of NLRP10 in the host immune response and found that Nlrp10−/− dendritic cells (DCs) elicited sub-optimal IFNγ production by antigen-specific CD4+ T cells compared to wild-type (WT) DCs. In response to T-cell encounter, CD40 ligation or Toll-like receptor 9 stimulation, Nlrp10−/− DCs produced low levels of IL-12, due to a substantial decrease in NF-κB activation. Defective IL-12 production was also evident in vivo and affected IFNγ production by CD4+ T cells. Upon Mycobacterium tuberculosis (Mtb) infection, Nlrp10−/− mice displayed diminished T helper 1-cell responses and increased bacterial growth compared to WT mice. These data indicate that NLRP10-mediated IL-12 production by DCs is critical for IFNγ induction in T cells and contributes to promote the host defense against Mtb.