%A Vacca,Maurizio %A Böhme,Julia %A Zambetti,Lia Paola %A Khameneh,Hanif Javanmard %A Paleja,Bhairav S. %A Laudisi,Federica %A Ho,Adrian W. S. %A Neo,Kurt %A Leong,Keith Weng Kit %A Marzuki,Mardiana %A Lee,Bernett %A Poidinger,Michael %A Santambrogio,Laura %A Tsenova,Liana %A Zolezzi,Francesca %A De Libero,Gennaro %A Singhal,Amit %A Mortellaro,Alessandra %D 2017 %J Frontiers in Immunology %C %F %G English %K NLRP10,Dendritic Cells,CpG DNA,Toll-Like Receptor 9,il-12,T helper 1,IFNg,Mycobacterium tuberculosis %Q %R 10.3389/fimmu.2017.01462 %W %L %M %P %7 %8 2017-November-02 %9 Original Research %+ Alessandra Mortellaro,Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR),Singapore,alessandra_mortellaro@immunol.a-star.edu.sg %+ Alessandra Mortellaro,Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore,Singapore,alessandra_mortellaro@immunol.a-star.edu.sg %# %! NLRP10 enhances the Th1 immune response %* %< %T NLRP10 Enhances CD4+ T-Cell-Mediated IFNγ Response via Regulation of Dendritic Cell-Derived IL-12 Release %U https://www.frontiersin.org/articles/10.3389/fimmu.2017.01462 %V 8 %0 JOURNAL ARTICLE %@ 1664-3224 %X NLRP10 is a nucleotide-binding oligomerization domain-like receptor that functions as an intracellular pattern recognition receptor for microbial products. Here, we generated a Nlrp10−/− mouse to delineate the role of NLRP10 in the host immune response and found that Nlrp10−/− dendritic cells (DCs) elicited sub-optimal IFNγ production by antigen-specific CD4+ T cells compared to wild-type (WT) DCs. In response to T-cell encounter, CD40 ligation or Toll-like receptor 9 stimulation, Nlrp10−/− DCs produced low levels of IL-12, due to a substantial decrease in NF-κB activation. Defective IL-12 production was also evident in vivo and affected IFNγ production by CD4+ T cells. Upon Mycobacterium tuberculosis (Mtb) infection, Nlrp10−/− mice displayed diminished T helper 1-cell responses and increased bacterial growth compared to WT mice. These data indicate that NLRP10-mediated IL-12 production by DCs is critical for IFNγ induction in T cells and contributes to promote the host defense against Mtb.