AUTHOR=Mardente Stefania , Mari Emanuela , Massimi Isabella , Tafani Marco , Guerriero Raffaella , Morsilli Ornella , Pulcinelli Fabio M. , Bianchi Marco E. , Zicari Alessandra 

TITLE=From Human Megakaryocytes to Platelets: Effects of Aspirin on High-Mobility Group Box 1/Receptor for Advanced Glycation End Products Axis

JOURNAL=Frontiers in Immunology

VOLUME=8

YEAR=2018

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2017.01946

DOI=10.3389/fimmu.2017.01946

ISSN=1664-3224

ABSTRACT=<p>Platelets (PLTs) are the major source of high-mobility group box 1 (HMGB1), a protein that is involved in sterile inflammation of blood vessels and thrombosis. Megakaryocytes (MKs) synthesize HMGB1 and transfer both protein and mRNA into PLTs and PLT-derived microvesicles (MV). Free HMGB1 found in supernatants of <italic>in vitro</italic> differentiated MKs and in a megakaryoblastic cell line (DAMI cells). Aspirin “<italic>in vivo</italic>” and “<italic>in vitro</italic>” not only reduces HMGB1 and receptor for advanced glycation end products expression on MKs and PLTs but also drives the movement of HMGB1 from MKs into PLTs and PLT-derived MV. These findings suggest that consumption of low doses of aspirin reduces the risk of atherosclerosis complications as well as reducing PLT aggregation by the inhibition of COX-1.</p>