%A Miles,Katherine %A Simpson,Joanne %A Brown,Sheila %A Cowan,Graeme %A Gray,David %A Gray,Mohini %D 2018 %J Frontiers in Immunology %C %F %G English %K regulatory B cells,Autoimmunity.,B1a cells,Apoptosis,tolerance mechanisms %Q %R 10.3389/fimmu.2017.01952 %W %L %M %P %7 %8 2018-January-19 %9 Original Research %+ Mohini Gray,MRC Centre for Inflammation Research, Queen’s Medical Research Institute, University of Edinburgh,United Kingdom,mohini.gray@ed.ac.uk %# %! Regulatory B cells are primarily B1a cells. %* %< %T Immune Tolerance to Apoptotic Self Is Mediated Primarily by Regulatory B1a Cells %U https://www.frontiersin.org/articles/10.3389/fimmu.2017.01952 %V 8 %0 JOURNAL ARTICLE %@ 1664-3224 %X The chronic autoimmune inflammatory diseases, systemic lupus erythematosus and Sjogren’s syndrome, develop when tolerance to apoptotic cells (ACs) is lost. We have previously reported that this tolerance is maintained by innate-like, IL-10 secreting regulatory B cells. Two questions remained. First, do these regulatory B cells belong predominantly to a single subset of steady-state B cells and second, what is their specificity? We report here that innate-like B cells with markers characteristic for B1a cells (CD43+veCD19hiCD5+veIgMhiIgDlo) constitute 80% of splenic and 96% of peritoneal B cells that respond to ACs by secreting IL-10. AC responsive B1a cells secrete self-reactive natural antibodies (NAbs) and IL-10, which is augmented by toll-like receptor (TLR) 7 or TLR9 stimulation. In so doing, they both accelerate the clearance of dying cells by macrophages and inhibit their potential to mount proinflammatory immune responses. While B1a cells make prolonged contact with ACs, they do not require TIM1 or complement to mediate their regulatory function. In an animal model of neural inflammation (experimental autoimmune encephalomyelitis), just 105 activated B1a B cells was sufficient to restrain inflammation. Activated B1a B cells also induced antigen-specific T cells to secrete IL-10. Hence, regulatory B1a cells specifically recognize and augment tolerance to apoptotic self via IL-10 and NAbs; but once activated, can also prevent autoimmune mediated inflammation.