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Front. Immunol. | doi: 10.3389/fimmu.2018.00287

Influence of intestinal indigenous microbiota on intrafamilial infection by Helicobacter pylori in Japan

 Takako Osaki1*, Cynthia Zaman1,  Yingsong Lin2,  Hideo Yonezawa1, Masumi Okuda3, 4, Eriko Nozaki5, Fuhito Hojo6,  Tomoko Hanawa1,  Satoshi Kurata1, Shogo Kikuchi2 and  Shigeru Kamiya1
  • 1Department of Infectious Diseases, Kyorin University, Japan
  • 2Department of Public Health,, School of Medicine, Aichi Medical University, Japan
  • 3Department of Pediatrics, School of Medicine, Aichi Medical University, Japan
  • 4Department of General Medicine and Community Health Science, Hyogo College of Medicine, Japan
  • 5Core laboratory for proteomics and genomics, Kyorin University School of Medicine, Japan
  • 6Institute of Laboratory Animals, Kyorin University School of Medicine, Japan

Helicobacter pylori is a causative pathogen of chronic gastritis, gastric ulcer disease and gastric cancer. Humans are known to be a natural host for H. pylori, and tend to acquire the pathogen before the age of 5 years. The infection may then persist lifelong if eradication therapy is not applied. One of the modes of transmission of H. pylori is between family members and therefore the presence of infected family members is an important risk factor in children. However, other environmental factors have not been fully analyzed. The present study was performed to clarify whether and to what extent intestinal microbiota affect H. pylori intrafamilial infection.
The fecal specimens from H. pylori-infected infants and H. pylori-infected and non-infected family members were collected in cohort studies conducted by Sasayama City, Hyogo Prefecture from 2010 to 2013. In total, 18 fecal DNA from 5 families were analysed. Samples were amplified using 16S rRNA universal primers and the amplicons were sequenced using the Ion PGM system.
PCoA analysis demonstrated that there was no difference in intestinal microbiota between H. pylori-positive and H. pylori-negative groups. In intrafamilial comparison tests, the Manhattan distance of intestinal microbiota between the H. pylori-infected infant proband and H. pylori-negative mother was nearest in the family with low intestinal microbial diversity. However, in the family with the highest intestinal microbial diversity, the nearest Manhattan distance was shown between the H. pylori-infected infant proband and H. pylori-infected mother. The results in this study showed that the composition of the intestinal microbiota was very similar between members of the same family and as such, colonization with organisms highly similar to the infected parent(s) may be a risk factor for H. pylori infection in children.

Keywords: Helicobacter pylori, Intrafamilial infection, intestinal microbiota, Mother to child transmission, diversity of microbiota

Received: 30 Oct 2017; Accepted: 01 Feb 2018.

Edited by:

Vida Abedi, Geisinger Health System, United States

Reviewed by:

Daniela Fiocco, University of Foggia, Italy
Matteo A. Russo, IRCCS San Raffaele Pisana, Italy  

Copyright: © 2018 Osaki, Zaman, Lin, Yonezawa, Okuda, Nozaki, Hojo, Hanawa, Kurata, Kikuchi and Kamiya. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: PhD. Takako Osaki, Kyorin University, Department of Infectious Diseases, Mitaka, Japan, osaki@ks.kyorin-u.ac.jp