Glucocorticoid-Induced Leucine Zipper Inhibits Interferon-Gamma Production in B Cells and Suppresses Colitis in Mice

Glucocorticoid-induced leucine zipper (GILZ) is transcriptionally upregulated by glucocorticoids (GCs) and mediates many of the anti-inflammatory effects of GCs. Since B cell activity has been linked to cytokine production and modulation of inflammatory responses, we herein investigated the role of GILZ in B cells during colitis development. B cell-specific gilz knock-out (gilz B cKO) mice exhibited increased production of the pro-inflammatory cytokine IFN-γ in B cells, and consequently CD4+ T cell activation. Increased IFN-γ production in B cells was associated with enhanced transcriptional activity of the transcription factor activator protein-1 (AP-1) on the IFN-γ promoter. Moreover, GILZ deficiency in B cells was linked to enhanced susceptibility to experimental colitis in mice, and this was reversed by administering GILZ protein. Interestingly, we observed increased production of IFN-γ in both B and T cells infiltrating the lamina propria (LP) of gilz B cKO mice. Together, these findings indicate that GILZ controls IFN-γ production in B cells, which also affects T cell activity, and increased production of IFN-γ by B and T cells in LP is associated with predisposition to inflammatory colitis in mice.

domains comprising a transforming growth factor (TGF)-βstimulated clone (TSC) box, a central LZ domain, and a proline (P)/glutamic acid (E)-rich (PER) region in the C-terminal part (10). Unlike most of LZ-containing proteins, GILZ does not contain a DNA-binding basic region. GILZ is mostly located in the cytoplasm, where it interacts with several signaling molecules and transcription factors including activator protein-1 (AP-1), a transcription factor pivotal for the activation of immune cells during inflammation (11). Indeed, GILZ heterodimerizes with both the c-Fos and c-Jun components of AP-1 (12), and over-expression of GILZ inhibits interleukin (IL)-2 production, a cytokine that plays a central role in T cell homeostasis and activation (4,10,13). Conversely, T cell activation suppresses GILZ expression (4,13,14), and this reciprocal inhibitory activity between T cell activation and GILZ expression indicates that GILZ modulates T cell activity, suggesting that altering GILZ expression may affect inflammatory processes such as inflammatory bowel diseases (IBDs). Indeed, we observed that over-expression of GILZ in T cells in GILZ transgenic (TG) mice induces downregulation of T helper (Th)-1 cells and upregulation of Th-2 cells (15,16). This correlates with inhibition of pathogenic activity in CD4 + T lymphocytes in intestinal lamina propria (LP), and decreased susceptibility to Th1-mediated colitis in mice overexpressing GILZ (17).
Inflammatory bowel diseases such as Crohn's disease (CD) and ulcerative colitis are chronic and progressive diseases of the gastrointestinal tract. Despite intensive research, our understanding of the pathogenesis of IBDs remains incomplete. T cells are known to play a key role in the pathogenesis of IBDs, and a more intensive Th1 cell response is observed in IBD patients (18,19).
The role of B cells in IBD is less clear, although they play an important role in controlling mucosal homeostasis in the gut, including antibody (Ab) production, antigen presentation, and co-stimulation of T lymphocytes (20,21). In addition to their role as conventional Ab-producing B cells, experimental evidence shows that cytokine production by novel subsets of B cells may also affect immune regulatory functions. For instance, IL-10-producing B cells, also called regulatory B (Breg) cells, play an essential role in modulating inflammation and autoimmunity (22). When stimulated, B cells may produce a wide range of cytokines such as IL-4, IL-17, and IFN-γ (23)(24)(25), thereby influencing the responses mediated by effector CD4 + T cells (26,27). However, the factors involved in the activation, expansion, and function of cytokine-producing B cells remain poorly characterized.
Recently, we demonstrated an important role of GILZ in B cell survival (28). We showed that lack of GILZ in mice in which B cell homeostasis was perturbed resulted in B cell lymphocytosis (28). In this study, we investigated whether GILZ expression in B cells contributes to the control of inflammatory processes in the gut, such as the production of pro-and/or anti-inflammatory cytokines, and explored whether this alters the severity of colitis in mice. We found that GILZ regulates IFN-γ expression in B cells, and GILZ-deficient B cells produced more IFN-γ, associated with increased AP-1 transcriptional activity. Increased IFNγ production by B cells lacking GILZ skewed wild-type (WT) CD4 + T lymphocytes toward a Th1 phenotype, increased IFN-γ production, and enhanced susceptibility to experimental colitis in mice.

Mice
Mice bearing a floxed gilz allele were generated as described previously (29) and maintained in a C57Bl/6J background. B-conditional gilz knock-out (KO) animals (gilz B cKO) were obtained by crossing mice bearing gilz flox alleles with transgenic mice bearing the CD19-CRE transgene (30), resulting in the deletion of gilz specifically in B cells ( Figure S1 in Supplementary Material), as described previously (28). Animal care was in compliance with regulations in Italy (DL 26/2014) and Europe (EU Directive 2010/63/EU).

Dinitrobenzene sulfonic acid (DnBs)-induced colitis
To induce colitis, 10-to 14-week-old C57BL/6 male mice were anesthetized with sodium thiopental (30 mg/kg) and xylazine (10 mg/kg). A 2 mg sample of 2,4-DNBS (Sigma-Aldrich) or vehicle (50% ethanol) was administered intrarectally. Body weight, stool consistency, and rectal bleeding were examined daily, and total clinical score was calculated as described previously (17). Disease score was evaluated as described previously (17) according to the following criteria: 0 = no damage, 1 = localized hyperemia without ulcers, 2 = linear ulcers with no significant inflammation, 3 = linear ulcers with inflammation at one site, 4 = two or more major sites of inflammation and ulceration extending greater than 1 cm along the length of the colon. To assess whether GILZ protein administration protected against the development of colitis, mice receiving DNBS were randomized to receive no treatment (DNBS alone) or 0.5 mg/kg TAT-GILZ fusion protein administered intraperitoneally for 3 days, starting on the same day as DNBS rectal administration.

Blood collection and hematocrit
Blood was collected from the retro-orbital plexus of anesthetized mice for hematocrit determination and FC analysis. Blood collection tubes were prepared with 20 µl of 0.5 M EDTA (pH 8). Analyses were performed using a Hemavet 950 (Drew Scientific) calibrated for mouse blood. Parameters determined included white blood cell (WBC) count, neutrophil count, lymphocyte count, monocyte count, eosinophil count, and basophil count.
isolation and culturing of B and T lymphocytes B and T cells for in vitro assays were isolated from spleen tissue of 10-to 14-week-old WT and gilz B cKO mice using Dynabeads Mouse CD43 Untouched B Cells and a Dynabeads Untouched Mouse CD4 Cell Kit (Thermo Fisher Scientific), respectively, following the manufacturer's instructions. For in vitro cultures, cells were maintained at a density of 2 × 10 6 /ml for 30 min or 5 h at 37°C in fully supplemented RPMI medium, with or without stimulation with 50 ng/ml phorbol 12-myristate 13-acetate (Sigma-Aldrich), 1 µg/ml ionomycin (Sigma-Aldrich), and 10 µg/ml lipopolysaccharide (Sigma-Aldrich).

lP cell isolation
Segments of the colon were incubated for 15 min at 37°C with Dissociation buffer consisting of Ca/Mag-free RPMI containing 5 mM EDTA, 10 mM HEPES, and 1 mM dithiothreitol (DTT), then extensively washed with complete RPMI containing 10 mM HEPES and 10 mM 10% fetal bovine serum (FBS). Fragments were then incubated with pre-warmed Digestion buffer (RPMI complete containing 0.5 mg/ml collagenase and 0.5 mg/ml DNaseI) for 20 min at 37°C. After washing, cells were subjected to density gradient centrifugation in 40/80% Percoll, and LP cells harvested from the interface were washed with phosphate-buffered saline (PBS) containing 2% FBS. Freshly isolated cells were resuspended in PBS for flow cytometric analysis.

enzyme-linked immunosorbent assay (elisa)
Analysis of Abs in sera collected from 12-to 18-month-old mice was performed by ELISA using IL-4, IL-10, IL-17, and IFN-γ ELISA Kits (Alpha Diagnostic International) according to the manufacturer's instructions.

abs and Flow cytometry
Monoclonal antibodies used for flow cytometry analyses and in vitro assays are listed in Table S1 in Supplementary Material. Analyses were performed using the ATTUNE NxT three-laser standard configuration (Life Technologies), and data were analyzed using Flow Jo software (Tree Star).

chromatin immunoprecipitation (chiP) assay
Chromatin immunoprecipitation assays were performed as described previously (31). In brief, cells were fixed in 1% PFA and sonicated on ice. Pre-cleared lysates were incubated overnight at 4°C with polyclonal anti-c-Jun (Abcam), polyclonal anti-acetyl histone H3 (AcH3, Millipore), or control polyclonal rabbit IgG (Cell Signaling). Immunocomplexes were collected using a Chip Assay Kit (Millipore), and qPCR analysis was performed using Power SYBR Green PCR Master Mix (Applied Biosystems). The following primers were used for chip analysis of the IFN-γ promoter: for the c-Jun binding site, For = GCTGTCTCATCGTCAGAGAGCCCA, Rev = TGATC GAAGGCTCCTCGGGATTACG; for the control region (CR), For = CTTAGGCTGGGAGGTTGTGT, Rev = TTCTCACTC CCTCCTACCCA.

statistical analysis
All statistical analysis was performed with Prism 6.0 (GraphPad). Results shown in figures are representative of at least three independent experiments unless otherwise indicated (n = x refers to the total number of animals used). The nonparametric Mann-Whitney U test or two-tailed unpaired Student's t-test was used for statistical comparison (*p < 0.05; **p < 0.005; ***p < 0.001).

B cell-specific gilZ KO (gilz B cKO) Mice exhibit B cell lymphocytosis
We previously reported that GILZ is expressed in B cells and regulates spontaneous apoptosis, thereby contributing to the control of B cell homeostasis (28). To confirm the cell-intrinsic role of GILZ in B cells, we crossed mice bearing the floxed GILZ allele with mice in which Cre recombinase is expressed under the control of the CD19 promoter (30), thus achieving a B cellrestricted GILZ deletion (hereafter referred to as gilz B cKO mice; Figure S1 in Supplementary Material). As observed for mice with GILZ deletion in all tissues (28), total WBC and lymphocyte counts were increased in the peripheral blood of gilz B cKO mice compared with WT mice (Figure 1A). Spleen tissue from gilz B cKO mice also displayed increased cellularity (Figure 1B), associated with increased frequency of B220 + cells compared with WT mice (Figure 1C). The number of B cells was significantly higher in the spleens of gilz B cKO mice compared with WT mice, while the number of blood cells of other myeloid and lymphoid lineage, including CD4 + , CD8 + , and Mac-1 + , remained constant. Similarly, no difference was found in CD4 + regulatory T (Treg) cell frequency between WT and gilz B cKO mice ( Figure 1D, last two columns on the right). These results demonstrate that the increase in the number of B cells following B cell-specific GILZ deletion in gilz B cKO mice is cell-intrinsic to the B cell population.
We next evaluated whether the absence of GILZ alters the frequency of B cell subsets within lymphoid tissues, including Breg cells. Flow cytometry analysis of splenic B cells revealed a similar frequency and number of Breg cells in WT and gilz B cKO mice In addition, we did not detect any difference in the frequency or number of plasma cells (defined as B220 + IgD lo CD138 hi ; Figure  S3B in Supplementary Material) or serum levels of immunoglobulins ( Figure S3C in Supplementary Material) in WT and gilz B cKO mice. Furthermore, we did not detect differences in the activation status of B cells isolated from spleens of WT and gilz B cKO mice, as measured by the expression of activation markers CD80 and CD86 ( Figure S4 in Supplementary Material). Together, these results suggest that the relative composition of B cell subsets was not perturbed by the absence of GILZ.

lack of gilZ in B cells results in increased iFn-γ Production
To assess the possible role of GILZ in Ab-independent functions of B cells, we evaluated cytokine production by splenic B cells in mice under physiological conditions. Figures 2A,B shows that lack of GILZ in B cells resulted in increased production of IFN-γ by B cells, but not other cytokines such as IL-4, IL-10, and IL-17. Consistently, increased IFN-γ production was detected in sera from aged gilz B cKO cells compared with WT mice, while expression of IL-17, IL-10, and IL-4 was not significantly different ( Figure 2C). These results suggest that GILZ is important in restraining the expression of IFN-γ in B cells, and lack of GILZ results in the elevated systemic presence of IFN-γ in gilz B cKO mice.

lack of gilZ in B cells stimulates iFn-γ Production in WT cD4 + T cells
Since IFN-γ influences the function of CD4 + T cells (33), we tested whether increased IFN-γ production by GILZ-deficient B cells results in altered T cell activity in gilz B cKO mice. We measured the expression of various cytokines in splenic CD4 + T cells in WT and gilz B cKO mice by flow cytometry. Figure 3A shows that expression of IFN-γ was significantly increased in T cells isolated from spleens of gilz B cKO mice compared with WT mice, while expression of IL-4, IL-10, and IL-17 did not differ significantly. To test whether increased IFN-γ expression in WT T cells results from the co-presence of GILZ-deficient Frontiers in Immunology | www.frontiersin.org July 2018 | Volume 9 | Article 1720 B cells producing IFN-γ, we co-cultured T cells purified from WT mice with B cells purified from gilz B cKO mice, but this did not significantly alter the total number of cultured cells in vitro ( Figure 3B). Flow cytometry analysis of cytokine production showed that co-culturing with IFN-γ-producing GILZ-deficient B cells ( Figure 3C) significantly increased the expression of IFN-γ in WT CD4 + T cells ( Figure 3D) without affecting the expression of other cytokines such as IL-17 and IL-10 ( Figure S5 in Supplementary Material). Importantly, addition of anti-IFN-γ Abs to cell cultures (34,35) counteracted the increased IFN-γ production by WT T cells co-cultured with gilz B cKO B cells ( Figure 3E). These results demonstrate that IFN-γ produced by GILZ-deficient B cells stimulates IFN-γ production in T cells.

gilZ inhibits aP-1-Dependent iFn-γ Production
Activation of the transcription factor AP-1 is important for the modulation of IFN-γ production by effector Th1 and by B cells (36,37), and GILZ binds directly to AP-1 and represses its transcriptional activity in T lymphocytes (12). We found that c-Jun, an integral part of the AP-1 transcription factor, binds more extensively to the IFN-γ promoter in purified B cells lacking GILZ ( Figure 4A) compare with WT B cells, as revealed by ChIP in B cells purified from spleens of WT or gilz B cKO animals. Consequently, the IFN-γ promoter is more transcriptionally active, as indicated by the enhanced acetylation of histone H3 (AcH3) in gilz B cKO B cells compared with WT B cells ( Figure 4B). This was associated with the enhanced nuclear translocation of c-Jun in GILZ-deficient B cells compared with WT B cells, as assessed by WB analysis of c-Jun levels in nuclear cell fractions ( Figure 4C). These results suggest that GILZ restrains IFN-γ production by inhibiting nuclear translocation of AP-1, and thus its transcriptional activity in B cells.

DnBs-induced colitis is More severe in gilz B cell-Deficient cKO Mice
While the pathogenic role of T cells in IBD is well established (38,39), the role of B cells in IBD is less clear, and the mechanisms involved in the interplay between B and T cells in controlling inflammatory responses in the gut are not well defined. The above results suggest that GILZ-deficient B cells drive T cells toward the Th1 phenotype. Th1 cells are implicated in IBDs and the role of B cells in IBDs has been highlighted in various mouse models (40,41). To assess whether the loss of GILZ in B cells influences the development of colitis, we used the DNBS-induced colitis Th1type disease mouse model. We found that lack of GILZ in B cells exacerbates DNBS-induced colitis in gilz cKO mice, as evidenced by a significantly increased body weight loss ( Figure 5A) and  an elevated disease index score in gilz cKO mice ( Figure 5B). Differences in colon weight/length ratio, a marker of tissue edema (Figure 5C), obvious reddening and shortening of the colon (Figure 5D), and disrupted colon tissue architecture ( Figure  S6 in Supplementary Material), were evident in gilz cKO mice compared with WT controls upon induction of colitis, providing further evidence of increased inflammation in experimental colitis upon GILZ deletion in mice.
To probe the basis of the increased disease susceptibility in gilz cKO mice, we compared cytokine production by cells isolated from the colon LP of WT and gilz cKO mice treated with DNBS. Similar to the spleen tissue results, we observed increased IFN-γ production by B cells in colon LP of gilz cKO compared with WT controls following administration of DNBS (Figures 6A,B). We also detected increased IFN-γ production by T cells in the LP of gilz cKO animals (Figures 6C,D). Together, these results suggest that increased IFN-γ production in the colon LP is associated with augmented susceptibility to experimental colitis in gilz cKO mice.

TaT-gilZ restores normal iFn-γ Production and Protects against DnBsinduced colitis
The above data indicate that GILZ is required for controlling the responses to inflammatory processes in the gut. To test whether exogenous GILZ administration can reverse colitis symptoms, we treated both WT and gilz cKO mice with recombinant TAT-GILZ fusion protein and evaluated DNBS-induced disease development. We previously demonstrated that recombinant TAT-GILZ can enter cells using the Hiv-TAT sequence that delivers the fusion protein into cells, in this case GILZ linked to the TAT transduction domain (9,17). As shown in Figure 7, the intra-peritoneal delivery of TAT-GILZ protein protected against DNBS-induced colitis in mice, as evidenced by diminished body weight loss in WT and gilz cKO mice treated with TAT-GILZ compared with non-treated controls ( Figure 7A). As expected, disease score ( Figure 7B) and colon weight/length ratio ( Figure 7C) indicated that treatment with TAT-GILZ decreased disease severity in WT mice (Figures 7B,C, column 5 vs. 3). Interestingly, TAT-GILZ treatment also counteracted the inflammation induced by DNBS in gilz cKO mice (Figures 7B,C, column 6 vs. 4), suggesting that recombinant TAT-GILZ administration had an anti-inflammatory effect and reversed inflammatory disease predisposition in gilz cKO mice.

DiscUssiOn
Despite recent advances in IBD therapy, an ideal treatment remains elusive, and therapy must often be discontinued due to serious side effects. For example, GCs are highly efficacious in therapeutic approaches to IBD, but prolonged treatment causes severe adverse effects that necessitate therapy interruption (1,2).
The Ab-independent pathogenic role of B cells is emerging as a key player in the development of chronic inflammatory and autoimmune diseases. This immunomodulatory function of B cells is associated with their ability to produce anti-or proinflammatory cytokines. Dysfunction of specific B cell subsets has been linked to the development or exacerbation of various diseases, including IBDs (42,43). A decrease in the abundance of B cells producing IL-10 (Breg cells) has been observed in patients with CD (44), and adoptive transfer of IL-10-producing B cells ameliorates intestinal inflammation in a mouse model of colitis (44). B cells can also produce pro-inflammatory cytokines, and perturbation of cytokine-producing B cell subsets has been linked to the pathology of inflammatory and autoimmune diseases (20,22,25,36). However, the mechanisms regulating the production of immuno-regulatory cytokines by B cells, and their role in the immunopathology of IBDs, remain undefined.
T and B cells are essential regulators of adaptive immune responses, and a growing body of evidence suggests that B cells possess immunomodulatory functions due to the production of specific cytokines and their interactions with T cell subpopulations. Through cytokine production, B cells promote the differentiation and maintenance of Treg cells and differentiation of other T cell subsets, including Th1, Th2, and Th17 (26,45,46). Importantly, B cells can stimulate Th1-type immunity by producing IFN-γ, as demonstrated in Salmonella typhimurium infection experiments (47).
In this study, we aimed to address the role GILZ, which is induced by GCs, in B cells using a B cell conditional gilz KO (B cKO) mouse model. We previously demonstrated that lack of GILZ expression modulates B cell homeostasis (28). Herein, we showed that restriction of gilz deletion to B cells recapitulates the B cell lymphocytosis phenotype, as demonstrated by the increased number of B220 + cells in the spleen and peripheral blood of gilz B cKO mice compared with WT controls. Moreover, gilz B cKO mice exhibited increased susceptibility to experimental colitis. The regulatory activities of a subset of B cells, namely Breg cells, have been linked to the modulation of inflammation and related diseases (42,44). Analysis of B cells in WT and gilz B cKO mice revealed that ablation of gilz expression in B cells does not perturb the frequency of CD1 hi CD5 + Breg cells, or production of their regulatory cytokines such as IL-10 and TGF-β. Similarly, gilz deficiency in B cells did not affect the production of Treg cells, even though lack of gilz specifically in T cells results in impaired generation of Treg cells (31). These observations indicate that deregulation of Breg and Treg functions, both shown to be important modulators of inflammatory diseases including IBDs, is not the mechanism responsible for increased inflammation in gilz B cKO mice.
B cells can act as accessory cells that regulate T cell differentiation and cytokine production (40,41). In this study, we observed that lack of GILZ in B cells led to increased production of IFN-γ in splenic B cells, but not of other cytokines such as IL-4 or IL-17. Increased IFN-γ levels were also detected in sera of aged gilz B cKO mice compared with WT mice, suggesting an increase in IFN-γ may be relevant to inflammatory processes, although no other clear signs of inflammation at steady state were observed in aged mice. Interestingly, we found that T cells also contributed   to the increased production of IFN-γ in gilz B cKO mice, and in vitro co-culture experiments confirm that WT T cells were stimulated to produce IFN-γ by gilz-deficient B cells. To further demonstrate that GILZ-deficient IFN-γ-producing B cells influenced the production of IFN-γ by WT T cells, we used two different anti-IFN-γ blocking Abs in an attempt to reverse the hyper-production of IFN-γ by T cells co-cultured with gilz B cKO B cells. The observed pharmacological rescue of normal IFN-γ production by the anti-IFN-γ Abs indicates that GILZ controls CD4 + T cell activity via B cell production of IFN-γ. These results suggest that GILZ influences the fine-tuning of B/T cell interactions, with consequent regulation of adaptive immune responses, such as the production of the pro-inflammatory cytokine IFN-γ by both B and T lymphocytes.
Glucocorticoid-induced leucine zipper is a leucine zipper protein that physically interacts with and modulates the functions of several other molecules (8, 10, 12-14, 29, 31). Various transcription factors regulate the activity of the ifng promoter, as revealed by bioinformatic analysis and previous studies (48). Among these, the AP-1 transcription factor plays an essential role in ifng transcriptional regulation, and at least two canonical AP-1 binding sites are present in the ifng proximal promoter. GILZ is able to interact with and inhibit the activity of AP-1 (12). We therefore assessed the activity of the ifng promoter and detected an increased AP-1 presence and enhanced acetylation of histone H3 in the proximity of the AP-1 binding site in GILZ-deficient B cells compared with WT B cells, suggesting that the enhanced activity of the ifng promoter in GILZ-deficient B cells is mediated by the AP-1 transcription factor. These results indicate that GILZ regulates AP-1 binding to the IFN-γ promoter, and hence its transcriptional activity.
To demonstrate that lack of GILZ in B cells affects functional interactions between B and T cells during inflammatory processes in vivo, we used DNBS-induced Th1-type experimental colitis model and monitored signs of disease development in WT and gilz B cKO mice. The results clearly showed that lack of GILZ was associated with exacerbated DNBS-induced colitis, demonstrating that GILZ acts as an anti-inflammatory protein in this cellular context, and that its activity in B cells is important for restraining pathological inflammation of the colon. Interestingly, we found that colitis in mice was antagonized by the in vivo administration of recombinant GILZ protein, in both WT and gilz B cKO mice, consistent with previous findings in other experimental mouse models of inflammatory diseases (9,17,49).
Increased leukocyte infiltration into the gut, and elevated production of inflammatory cytokines such as IFN-γ, are characteristic features of IBDs in humans and other animals (50). We detected an increase in IFN-γ production in cells isolated from colon LP of gilz B cKO mice following DNBS-induced colitis. Interestingly, we confirmed that T cells also contribute to the increased production of IFN-γ in colon LP in gilz B cKO mice in response to DNBS treatment, suggesting that the aberrant function of B cells determined by GILZ deletion enhances the pro-inflammatory activity of B and T cells in the colon during disease development. These findings point to an important Ab-independent function of B cells that modulate T cell responses in inflammatory diseases. Given that increased IFN-γ production in T cells is observed not only in colon LP but also in spleen tissue, we cannot exclude the possibility that T cells are primed by B cells in peripheral lymphoid organs, leading to their enhanced proinflammatory activity in LP. Further studies aimed at dissecting the mechanisms of potential cross talk between B and T cells in disease development are therefore warranted.
In conclusion, the results of this study indicate that GILZ regulates IFN-γ production in B cells by controlling AP-1 transcriptional activity, and modulation of GILZ expression is important for regulating B and T cell activity and the development of inflammatory diseases of the GI tract, such as colitis. Therefore, GILZ could represent a novel target for therapies aimed at treating inflammatory diseases such as IBDs. aUThOr cOnTriBUTiOns SB participated to study concept and design and supervision; he designed and performed the experiments and analyzed the data. He wrote the manuscript. He obtained funding to support the project leading to this publication. DS, SF, and AG performed the experiments and analyzed the data. FA performed the experiments and statistical analysis of the data. SR performed the experiments and participated to critical revision of the manuscript for important intellectual content. GM participates to the drafting of the manuscript and to critical revision of the manuscript for important intellectual content. She obtained funding to support the project leading to this publication. OB performed the experiments and analyzed the data. She participates to the drafting of the manuscript and to critical revision of the manuscript for important intellectual content. She obtained funding to support the project leading to this publication. CR made substantial contributions to study concept and design and supervision; he also participates in drafting the article or revising it critically for important intellectual content. He obtained funding to support the project leading to this publication.