%A Wikenheiser,Daniel J.
%A Brown,Susie L.
%A Lee,Juhyung
%A Stumhofer,Jason S.
%D 2018
%J Frontiers in Immunology
%C
%F
%G English
%K Malaria,T follicular helper cell (TFH),antibody,NK1.1+ cells,plasmablast
%Q
%R 10.3389/fimmu.2018.02277
%W
%L
%M
%P
%7
%8 2018-October-15
%9 Original Research
%#
%! NK1.1-expressing CD4+ T cells
%*
%<
%T NK1.1 Expression Defines a Population of CD4+ Effector T Cells Displaying Th1 and Tfh Cell Properties That Support Early Antibody Production During Plasmodium yoelii Infection
%U https://www.frontiersin.org/articles/10.3389/fimmu.2018.02277
%V 9
%0 JOURNAL ARTICLE
%@ 1664-3224
%X Early plasmablast induction is a hallmark of Plasmodium infection and is thought to contribute to the control of acute parasite burden. Although long understood to be a T-cell dependent phenomenon, regulation of early plasmablast differentiation, however, is poorly understood. Here, we identify a population of CD4+ T cells that express the innate NK cell marker NK1.1 as an important source of T cell help for early plasmablast and parasite-specific Ab production. Interestingly, NK1.1+ CD4+ T cells arise from conventional, naive NK1.1− CD4+ T cells, and their generation is independent of CD1d but critically reliant on MHC-II. CD4+ T cells that express NK1.1 early after activation produce IFN-γ and IL-21, and express the follicular helper T (Tfh) cell markers ICOS, PD-1 and CXCR5 more frequently than NK1.1− CD4+ T cells. Further analysis of this population revealed that NK1.1+ Tfh-like cells were more regularly complexed with plasmablasts than NK1.1− Tfh-like cells. Ultimately, depletion of NK1.1+ cells impaired class-switched parasite-specific antibody production during early Plasmodium yoelii infection. Together, these data suggest that expression of NK1.1 defines a population of rapidly expanding effector CD4+ T cells that specifically promote plasmablast induction during Plasmodium infection and represent a subset of T cells whose modulation could promote effective vaccine design.