Schistosomal Lipids Activate Human Eosinophils via Toll-Like Receptor 2 and PGD2 Receptors: 15-LO Role in Cytokine Secretion

Parasite-derived lipids may play important roles in host-pathogen interactions and immune evasion mechanisms. Remarkable accumulation of eosinophils is a characteristic feature of inflammation associated with parasitic disease, especially caused by helminthes. Infiltrating eosinophils are implicated in the pathogenesis of helminth infection by virtue of their capacity to release an array of tissue-damaging and immunoregulatory mediators. However, the mechanisms involved in the activation of human eosinophils by parasite-derived molecules are not clear. Here we investigated the effects and mechanisms of schistosomal lipids-induced activation of human eosinophils. Our results showed that stimulation of human eosinophils in vitro with total lipid extracts from adult worms of S. mansoni induced direct activation of human eosinophils, eliciting lipid droplet biogenesis, synthesis of leukotriene (LT) C4 and eoxin (EX) C4 (14,15 LTC4) and secretion of eosinophil pre-formed TGFβ. We demonstrated that main eosinophil activating components within S. mansoni lipid extract are schistosomal-derived lysophosphatidylcholine (LPC) and prostaglandin (PG)D2. Moreover, TLR2 is up-regulated in human eosinophils upon stimulation with schistosomal lipids and pre-treatment with anti-TLR2 inhibited both schistosomal lipids- and LPC-, but not PGD2-, induced lipid droplet biogenesis and EXC4 synthesis within eosinophils, indicating that TLR2 mediates LPC-driven human eosinophil activation. By employing PGD2 receptor antagonists, we demonstrated that DP1 receptors are also involved in various parameters of human eosinophil activation induced by schistosomal lipids, but not by schistosomal LPC. In addition, schistosomal lipids and their active components PGD2 and LPC, triggered 15-LO dependent production of EXC4 and secretion of TGFβ. Taken together, our results showed that schistosomal lipids contain at least two components—LPC and PGD2—that are capable of direct activation of human eosinophils acting on distinct eosinophil-expressed receptors, noticeably TLR2 as well as DP1, trigger human eosinophil activation characterized by production/secretion of pro-inflammatory and immunoregulatory mediators.

Parasite-derived lipids may play important roles in host-pathogen interactions and immune evasion mechanisms. Remarkable accumulation of eosinophils is a characteristic feature of inflammation associated with parasitic disease, especially caused by helminthes. Infiltrating eosinophils are implicated in the pathogenesis of helminth infection by virtue of their capacity to release an array of tissue-damaging and immunoregulatory mediators. However, the mechanisms involved in the activation of human eosinophils by parasite-derived molecules are not clear. Here we investigated the effects and mechanisms of schistosomal lipids-induced activation of human eosinophils. Our results showed that stimulation of human eosinophils in vitro with total lipid extracts from adult worms of S. mansoni induced direct activation of human eosinophils, eliciting lipid droplet biogenesis, synthesis of leukotriene (LT) C 4 and eoxin (EX) C 4 (14,15 LTC 4 ) and secretion of eosinophil pre-formed TGFβ. We demonstrated that main eosinophil activating components within S. mansoni lipid extract are schistosomal-derived lysophosphatidylcholine (LPC) and prostaglandin (PG)D 2 . Moreover, TLR2 is up-regulated in human eosinophils upon stimulation with schistosomal lipids and pre-treatment with anti-TLR2 inhibited both schistosomal lipids-and LPC-, but not PGD 2 -, induced lipid droplet biogenesis and EXC 4 synthesis within eosinophils, indicating that TLR2 mediates LPC-driven human eosinophil activation. By employing PGD 2 receptor antagonists, we demonstrated that DP1 receptors are also involved in various parameters of human eosinophil activation induced by schistosomal lipids, but not by schistosomal LPC. In addition, schistosomal lipids and their active components PGD 2 and LPC, triggered 15-LO dependent production of EXC 4 and secretion of TGFβ. Taken together, our results showed that schistosomal lipids contain at least two components-LPC and PGD 2 -that are capable of direct activation of human eosinophils acting on distinct eosinophil-expressed receptors, noticeably TLR2 as well as DP1, trigger human eosinophil activation characterized by production/secretion of pro-inflammatory and immunoregulatory mediators.
Keywords: eosinophil, PGD 2 , lipids, schistosoma, TLR, LPC, TGFβ, eoxin INTRODUCTION Schistosomiasis is a chronic parasitic infection caused by five species of trematode helminths of the genus Schistosoma that infects more than 200 million people in developing countries (1). The major pathologic manifestations of the chronic Schistosoma mansoni disease are the eosinophil-enriched granulomatous response usually accompanied by severe hepatic and periportal fibrosis, portal hypertension, and portosystemic shunting of venous blood (2). S. mansoni are complex multicellular parasites that evolved some unique processes which are vital for their long-term survival within the mammalian host. This worm is capable of secreting molecules that subvert or suppress host immune responses and its cover tegument acts as an immune refractory barrier (1,2). Schistosomal tegumental outer-surface structure appears to be critically involved in complex host-parasite interactions. Besides S. mansoni protein composition, schistosomal lipids have gained increased attention due to their important immunomodulatory properties (3)(4)(5)(6).The most predominant phospholipid in S. mansoni cercariae, schistosomula and adults worms is phosphatidylcholine (7). S. mansoni lipids are required by the parasite not only to maintain its surface integrity and structural requirements but also for egg production, cell-cell signaling, and modulation of immune system (3,8). Accordingly, employing a murine model of S. mansoni infection we have recently demonstrate that TLR2dependent pathways activated in vivo by schistosomal-derived lipids play an important immunomodulatory role contributing to the pathogenesis and lethality in the chronic phase of infection (3,4). Of note, we have shown that schistosomal-derived lipids, mostly lysophosphatidylcholine (LPC), were able to induce macrophage activation and polarization toward a M2 phenotype, and in vivo eosinophilic response (3,4).
Eosinophils play an important role in modulating the host immune response to helminth infections (9), and eosinophilia has been largely recognized as a characteristic host response during schistosomiasis (10). Accumulating evidence has established eosinophils as multifunctional leukocytes with varied effector and immunomodulatory functions not only in allergic or helminthic disease but also in the initiation and amplification of numerous inflammatory and infectious responses and as modulators of innate and adaptive immunity (11). Although the roles of eosinophils as a defense mechanism against S. mansoni infection have been challenged and remain controversial (12)(13)(14), eosinophils may play modulatory roles in maintaining the Th2 response to infection via cytokine secretion, and may contribute to the cytokine-mediated pathogenesis (15,16). Here we hypothesized that parasite-derived lipids may play roles in host-pathogen interactions by activating eosinophils to release immunomodulatory and pro-fibrotic mediators.

Purification and Analysis of S. mansoni Lipids
The total lipid extracts were isolated from adult worms of S. mansoni and lipids were extracted as describe (3). Briefly, lipids from S. mansoni worms were extracted for 2 h with a chloroform-methanol-water solution (2:1:0.8, v/v). After centrifugation, the supernatant was collected and the pellet subjected to a second lipid extraction for 1 h. Schistosomal total lipids (Schisto-TL) were used to stimulate eosinophils (see below) or were subjected to two dimensional thin-layer chromatography (TLC) for phospholipid fractionation and analysis and lysophospholipid (LPC) extraction. Schistosomal LPCs (Schisto-LPC) were removed from TLC using the method previously described (

Isolation of Human Blood Eosinophils
Peripheral blood was obtained with informed consent from healthy donors. Briefly, after dextran sedimentation and Ficoll gradient steps, eosinophils were isolated from contaminating neutrophils by negative immunomagnetic selection using the EasySep TM system (StemCell Technologies Inc.)(cell purity ∼99%; cell viability ∼95%) (17). The protocol was approved by ethical review boards of the Beth Israel Deaconess Medical Center Committee on Clinical Investigation and Federal University of Rio de Janeiro (Rio de Janeiro, Brazil).

Lipid Droplet Staining and Enumeration
Lipid droplets were stained and enumerated as previously described (18). For lipid droplet enumeration within eosinophil cytoplasm, cytospin cells were fixed in 3.7% formaldehyde (diluted in HBSS −/− ), rinsed in 0.1 M cacodylate buffer (pH 7.4), stained with 1.5% OsO 4 for 30 min, rinsed in distilled H 2 O, immersed in 1.0% thiocarbohydrazide for 5 min, rinsed in 0.1 M cacodylate buffer, restained with 1.5% OsO 4 for 3 min, rinsed in distilled water, and mounted. Lipid droplets were enumerated by light (osmium staining) microscopy. Fifty consecutively scanned eosinophils were evaluated in a blinded fashion by more than one individual, and the results were expressed as the number of lipid droplets per eosinophil.
Alternatively, analysis of lipid droplets was performed with Nile Red (Sigma-Aldrich) for better visualization of the cytoplasmic distribution of eosinophil lipid droplets. Briefly, while still moist, eosinophils on cytospin slides were fixed in 3.7% formaldehyde in HBSS −/− pH 7.4, rinsed with PBS buffer and then incubated with Nile Red for 30 min, rinsed in dH 2 O, incubated with DAPI (4 ′ ,6-Diamidino-2-phenylindole dihydrochloride) (Sigma-Aldrich) per 5 min, rinsed in dH 2 O, and then dried and mounted.
Expression of Perilipin 2 (PLIN2)/Adipose Differentiation-Related Protein (ADRP) and 15-LO Analysis of PLIN2/ADRP and 15-LO expression within human eosinophils were done by western blotting. In brief, eosinophil lysates were prepared in reducing and denaturing conditions and subjected to SDS-PAGE. Samples were submitted to electrophoresis in 5-15% acrylamide gradient SDS-PAGE gels. After transfer onto nitrocellulose membranes, non-specific binding sites were blocked with 5% non-fat milk in Tris buffered saline-Tween (TBST; 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.05% Tween 20). Membranes were probed with guinea pig polyclonal ADRP antibody (AP 002; Fitzgerald, MA); anti-15-LO-1 (Cayman Chemical), and anti-β-actin mAb (BD Transduction Laboratories) in TBST with 1% non-fat dry milk. Proteins of interest were then identified by incubating the membrane with HRP-conjugated secondary antibodies in TBST, followed by detection of antigen-antibody complexes by Supersignal Chemiluminescence (Pierce). The detection was done by exposing membranes to autoradiography film.

TLR2 Expression
Analysis of TLR2 expression was performed by flow cytometry analysis. Human eosinophils were washed with HBSS −/− and then incubated for 30 min with FITC-conjugated anti-TLR2 mAb (clone TL 2.1) or IgG2a k isotype control-FITC from eBioscience. After washings, cells were analyzed by flow cytometry in a FACS Calibur (BD Biosciences) flow cytometer.

EicosaCell for Intracellular EXC 4 Immunodetection
EicosaCell technique (19) was used to immunodetect EXC 4 at its intracellular synthesis sites. In vitro-stimulated human eosinophils were mixed with an equal volume of water-soluble 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide (EDAC; 0.2% in HBSS containing 1% BSA for 10 min) (Sigma), used to cross-link eicosanoid carboxyl groups to amines in adjacent proteins. Eosinophils, washed, cytospun onto glass slides and subjected to a blocking step (1%BSA for 30 min), were incubated with rabbit anti-EXC 4 Abs (Cayman Chemicals) overnight and secondary DyLight488 green fluorochrome anti-rabbit IgG (Jackson ImmunoResearch Laboratories) for 1 h. As specificity controls for the immunolocalization of EXC 4 , rabbit IgG (Sigma) was routinely included as a non-immune control for the primary anti-EXC 4 (with no detectable staining; data not shown). Mounting medium containing DAPI was applied to each slide before coverslip attachment to allow visualization of blue-stained eosinophil nuclei. Images were obtained using an Olympus BX51 fluorescence microscope at 100x magnification and photographs were taken with the Olympus 72 digital camera (Olympus Optical Co., Tokyo, Japan) in conjunction with CellF Imaging Software for Life Science Microscopy (Olympus Life Science Europa GMBH, Hamburg, Germany).

Eicosanoid Quantification
LTC 4 or EXC 4 (14,15 LTC 4 ) found in eosinophil supernatants and PGD 2 found in schistosomal lipid extracts were measured by specific commercial EIA kits, according to the manufacturer's instructions (Cayman).

Analysis of TGFβ Secretion
Cell-free supernatants from in vitro stimulated eosinophils were collected and stored at −20 • C until the day of analysis. Human TGFβ were measured by commercial ELISA kits, according to the manufacturer's instructions (R&D Systems).
In addition to the study of TGFβ release, the intracellular TGFβ levels were assessed by flow cytometry. Briefly, eosinophils were stimulated for 1 h with Schisto-TL (1µg/mL), Schisto-LPC (0.1µg/mL) or vehicle. After that, cells were fixed in 4% paraformaldehyde fixative (PFA), washed with PBS and incubated for 15 min with permeabilization buffer (saponin 0.05% in PBS containing 2% BSA). Cells were then washed and incubated for 30 min with FITC-labeled goat anti-TGFβ Ab from eBioscience (or FITC-labeled isotype) diluted in permeabilization buffer. After washings, cells were analyzed by flow cytometry in a FACSCalibur flow cytometer (BD Biosciences).

Statistical Analysis
Data are expressed as mean ± SEM of at least three independent experiments. Multiple comparisons among groups were performed by one-way ANOVA followed by Student-Newman-Keuls test, with the level of significance set at p < 0.05.

Schistosomal Lipids Directly Activate Lipid Droplet Biogenesis in Human Eosinophils in a TLR2 Dependent Manner
To verify if schistosomal lipids could directly trigger eosinophil activation, we isolated human eosinophils and stimulated them with schistosomal lipid extract (Schisto-TL) or purified schistosomal-derived lysophosphatidylcholine fraction (Schisto-LPC). Increased numbers of lipid droplets in eosinophils have been considered as markers of cell activation and have been associated with increased capacity of eosinophil eicosanoid synthesis (20,21). As shown in Figure 1, schistosomal lipids as well as purified schistosomal-derived LPC triggered significant increases in lipid droplet numbers and increased expression of the lipid droplet structural protein and marker PLIN2/ADRP after 1 h (Figures 1A,B,D) when compared to non-stimulated eosinophils. Since schistosomal lipids were described as a TLR2 signaling pathway activators in murine infections (3), we checked if human eosinophils were able to express TLR2. The results demonstrated that human eosinophils constitutively express cell surface TLR2, which was upregulated after stimulation with Schisto-TL or Schisto-LPC as assessed by FACS analysis (Figure 1C). Similarly, TLR2 mRNA levels assessed by RT-PCR were also upregulated by schistosomal lipids (Supplementary Figure 1). To confirm that TLR2 was playing a role in the lipid droplet biogenesis triggered by Schisto-TL and Schisto-LPC in eosinophils, we blocked TLR2 with a neutralizing antibody, and analyzed lipid droplet biogenesis as well as the levels of ADRP. Our results indicated that lipid droplet biogenesis and ADRP expression triggered by Schito-TL and Schisto-LPC occurred in a TLR2-dependent manner (Figures 1B,D).

Eosinophil Lipid Droplet Biogenesis Elicited by S. mansoni-Derived Lipids Is Also Mediated by DP1 Activation
Since it has been demonstrated that the helminth S. mansoni is capable of producing PGD 2 (22), we wondered if lipid droplet biogenesis triggered by Schisto-TL and Schisto-LPC could be mediated by PGD 2 receptors, DP1 and DP2, in addition to TLR2. To address this question, we treated human eosinophils with DP1 receptor antagonist BWA868c and DP2 receptor antagonist Cay10471 prior to stimulation with Schisto-TL and Schisto-LPC. Our results showed that schistosomal TL triggered DP1 dependent but DP2 independent lipid droplet biogenesis (Figure 2A). Of note, eosinophils stimulated with PGD 2 display similar DP1 dependent-but DP2 independent lipid droplet biogenesis, as previously reported [ Figure 2A and (23)]. In addition, treatment with an anti-TLR2 neutralizing antibody, while effective against Schisto-LPC (Figure 1D), failed to inhibit lipid droplet formation induced by PGD 2 (from 17.67 ± 0.10 to 19.5 ± 0.2 lipid droplets/eosinophil in PGD 2 compared to anti-TLR2 plus PGD 2 ; non-significant).
We also verified if LPC could trigger lipid droplet biogenesis in eosinophils signaling through DP1 receptors. However, different to what was observed with Schisto-TL-stimulated eosinophils, Schisto-LPC triggered DP1-independent lipid droplet formation ( Figure 2B). Additionally, we showed that PGD 2 induced lipid droplet formation in a dose-dependent manner, and we observed a synergistic effect in lipid droplet formation when eosinophils were stimulated along with subliminal concentrations of both PGD 2 and LPC ( Figure 2C). Of note, the presence of PGD 2 in the schistosomal lipid extract was confirmed by EIA (∼150 pg/mL of PGD 2 /Schisto-TL), indicating that Schisto-TL effects on eosinophils may be due to synergistic interactions between both lipids PGD 2 and Schsito-LPC present in the total lipid extract of S. mansoni.

Schistosomal-Derived Lipids Induce Leukotriene C 4 (LTC 4 ) and EXC 4 (14,15 LTC 4 ) Generation by Human Eosinophils
Considering that lipid droplets are major intracellular sites involved in eicosanoid synthesis during inflammatory conditions (21) and eosinophils are an abundant source of 5-LO and 15-LO derived lipid mediators, we analyzed if Schisto-TL and Schisto-LPC could activate generation of 5-LO-derived LTC 4 and 15-LO-derived EXC 4 by human eosinophils. Our results showed that both Schisto-TL and Schisto-LPC triggers LTC 4 and EXC 4 synthesis by human eosinophils (Figure 3A). Different from AA-or PGD 2 -stimulated eosinophils for which LTC 4 is the major leukotriene formed within eosinophils, the relative amounts of EXC 4 induced by Schisto-TL and Schisto-LPC were higher than LTC 4 amounts (Figures 3A,B). The increased EXC 4 levels induced by Schisto-TL and Schisto-LPC in comparison with AA stimulation could be also visualized intracellularly in human eosinophils as assessed by Eicosacell assay (Figure 3C). Detailed analysis revealed that most newly synthesized EXC 4 (green labeling) within Schisto-TL-, Schisto-LPC, and PGD 2stimulated eosinophils was in a punctate cytoplasmic pattern proximate to, but separate from, the nucleus and fully consistent in size and form with eosinophil lipid droplets ( Figure 3C).
In order to understand which receptor could be playing a role in EXC 4 generation by Schisto-TL and Schisto-LPC, human eosinophils were pre-treated with TLR2 blocking antibody prior to stimulation with Schisto-TL and Schisto-LPC. The results demonstrated that EXC 4 secretion induced by Schisto-TL or by Schisto-LPC occurred in a TLR2-dependent manner ( Figure 3D).
Eosinophils store pro-fibrogenic cytokines including TGFβ (27) in their intracellular granules. Among schistosomiasisrelated eosinophil functions, secretion of such fibrogenic cytokines as TGF-β appear to have roles in this hepatic granulofibrotic disease (28,29). Stimulation of human eosinophils with Schisto-TL and Schisto-LPC triggered rapid (within 1 h of stimulation) TGFβ secretion, as assessed by both decrease of intracellular preformed stores of TGFβ by flow Frontiers in Immunology | www.frontiersin.org   cytometry ( Figure 5A) and increase of released extracellular levels of TGFβ in human eosinophil supernatants (Figure 5B).
In order to study potential roles of eosinophil 15-LO-driven EXC 4 synthesis on TGFβ release from human eosinophils, we analyzed whether 15-LO inhibitor could impair schistosomal lipids-induced secretion of preformed TGFβ from human eosinophils. As shown in Figure 5B, our data indicated that both Schisto-TL-or Schisto-LPC-triggered TGFβ secretion were dependent of 15-LO activity, since 15-LO inhibition reduced eosinophil extracellular levels of TGFβ. As shown in Table 1, while either EXC 4 or LTC 4 failed to induce lipid droplet biogenesis within eosinophils, stimulation of human eosinophils by EXC 4 , but not by LTC 4 , lead to increased TGFβ release by human eosinophils (Figure 5C). Of note, EXC 4 -triggered TGFβ secretion was not modified by 15-LO inhibition (Figure 5C), therefore supporting EXC 4 -mediated autocrine activity of 15-LO-driven TGFβ release by human eosinophil stimulated with schistosomal lipids.

Discussion
Eosinophils are known to display defensive and proinflammatory activities in a variety of helminth infections. However, rather than effector cells of helminthiasis, eosinophils have recently emerged as immunomodulatory cells capable of  acting to maintain homeostasis, resolve inflammation, promote Th2 immune responses, and repair damaged tissues, by inducing, for instance, secretion of pro-fibrotic mediators like TGFβ. While mediators known to activate the effector functions of eosinophils in helminth infection-associated eosinophilia are mostly host-derived molecules, direct stimulation of eosinophils with helminth-derived molecules is poorly or not studied and may trigger such immunomodulatory eosinophils. Stimulatory molecules that trigger fine-tuned eosinophil activation characterized by secretion of eosinophil-derived molecules with pro-resolving/repairing, pro-fibrotic impacts remain largely uncharacterized, here; we showed that schistosomal derived lipids can directly stimulate human eosinophils to secrete pro-fibrotic TGFβ.
Here, we demonstrated that schistosomal lipids exhibit distinctive regulatory functions in activating arachidonic acid metabolism and cytokine release from human eosinophils. Both schistosomal-derived LPC and PGD 2 acting on specific membrane receptors, TLR2 and DP1 respectively, activated a 15-LO-driven intracellular pathway promoting lipid dropletcompartmentalized EXC 4 synthesis and rapid secretion of eosinophil preformed TGFβ (Figure 6). Full eosinophil secretory capability upon stimulation with schistosomal lipids (including LPC, PGD 2 and any other bioactive schistosomal lipid not or AA (as indicated). Lipid droplets were enumerated in 50 consecutive osmium-stained cells. Values are expressed as the mean ± SEM of at least three distinct donors. +p < 0.05 compared with non-stimulated eosinophils.
studied here) are far from fully characterized. Nevertheless, our study is pioneer in demonstrating that schistosomal lipids are indeed capable of directly activating secretion of immunomodulatory/pro-fibrotic molecules from human eosinophils.
Our current findings demonstrate that schistosomal LPC and PGD 2 , are recognized, respectively, by TLR2 and DP1 receptors on human eosinophils, to trigger secretion of at least three active molecules: EXC 4, LTC 4 , and TGFβ from eosinophils. While the last two eosinophil-derived molecules are well recognized mediators of eosinophil responses in several eosinophilic conditions as helminth infections, EXC 4 is a more recently described eicosanoid produced in high quantities by eosinophils through the 15-LO pathway associated with asthma (30,31). For EXC 4 there is no receptor identified to date and virtually no cellular activity has been described. Exception is the in vitro demonstration that, similar to receptor-mediated LTC 4 -induced effect, EXC 4 is able to directly increase cell permeability of human endothelial cells (30). Specifically regarding human eosinophil activation and again similar to LTC 4 (32), EXC 4 does not have the ability to trigger lipid droplet biogenesis and therefore may be unable to assemble the intracellular compartments of eicosanoid synthesis within eosinophils. On the other hand, EXC 4 was capable of inducing TGFβ secretion from human eosinophils (or mouse eosinophils; data not shown), showing that EXC 4 stimulation is not species-specific and triggers a specific pattern of eosinophil activation compatible with a receptor-initiated event. While other eosinophil stimulatory functions triggered by exogenous or eosinophil-derived 15-LO-driven EXC 4 are still pending characterization, our data shows that EXC 4 synthesis and TGFβ secretion are mediated by eosinophil 15-LO activity. In agreement, 15-LO has been show to participate in the regulation of cytokine expression and release in other cell types (33), in addition to involvement in the biosynthesis of proresolving lipids, such as lipoxins (31), resolvins (34), maresins (35), and protectins (36). Based in a history of autocrine (or even intracrine) activities of eosinophil-derived eicosanoids mediating eosinophil functions (37), as well as, EXC 4 ability to induce TGFβ secretion from eosinophils seen here, it would be reasonable to postulate that 15-LO-driven synthesized EXC 4 under schistosomal lipids-stimulation mediates subsequent TGFβ secretion by eosinophils. Further assays are needed to confirm such hypothesis, inasmuch as other 15-LO metabolites could participate in TGFβ release. Unfortunately, studies of putative autocrine activities of eosinophil-derived EXC 4 are still hampered by the lack of receptor identification and, therefore antagonist availability. On the other hand, the partial involvement of 15-LO on TGFβ secretion from eosinophils (since inhibition of 15-LO activity did not block completely TGFβ secretion) indicates that besides 15-LO-driven eosinophilderived molecules, 15-LO-independent eosinophil-derived mediators secreted by eosinophils upon schistosomal-derived lipids activation may also be regulating TGFβ secretion. Among 15-LO-independent eosinophil-derived molecules secreted upon schistosomal lipids-stimulation, we have discarded 5-LO metabolite LTC 4 as an autocrine/paracrine mediator of schistosomal lipids-induced TGFβ secretion, since in contrast to EXC 4 , LTC 4 failed to trigger TGFβ secretion from eosinophils. Although LTC 4 does not control TGFβ secretion by human eosinophils, cross-talks between leukotrienes and TGFβ have been appreciated in a variety of cell sources, including stellate hepatic cells, macrophages and eosinophils (38)(39)(40)(41)(42). In addition, concerning S. mansoni infection where IL-4-mediated Th2 immune response play major roles in pathology (2), LTC 4 can function as an eosinophil-derived signal that promotes IL-4 secretion from eosinophils (43,44). Accordingly, studies employing 5-LO deficient animals have demonstrated an immune-regulatory role for 5-LO in schistosomal infection by the demonstration of decreased levels of IL-4 and IL-13, and decreased granuloma size in 5-LO deficient animals, while exhibiting increased levels of TGFβ and fibrosis (45,46).
We have previously shown that schistosomal-derived lipid LPC induces in vivo eosinophil recruitment and activation in a murine model of S. mansoni infection via TLR2initiated signaling pathways (3). Moreover, TLR2 expression on human eosinophils have been already shown, however its activation is reported to trigger eosinophil effector functions, such as eosinophil degranulation of cytotoxic ECP protein and superoxide production (47), rather than immunomodulatory ones. More consonant with our data showing LPC/TLR2driven secretion of immunomodulatory molecules from human eosinophils, as recently reported, schistosomal LPC can induce macrophage activation and polarization toward the antiinflammatory M2 phenotype with TGFβ secretion (4).
Schistosomiasis is a major intravascular infection, and adult worm live in direct contact with the mesenteric or portal vein endothelium. Adult worms continuously expose, secrete and excrete numerous substances that may directly or indirectly activate eosinophils in the liver and eosinopoiesis in the bone marrow and/or extramedullary. Here we demonstrate that adult worm lipid extracts contain at least two molecules, LPC and PGD 2 , capable to directly activate human through TLR-2 and DP1 pathways, respectively. Adult worms also produce large numbers of intravascular eggs, and indeed schistosomal trapped eggs in the host tissue have important pathological roles in eosinophil-granulomatous inflammatory response, raising the intriguing question as whether schistosomal eggs have similar eosinophil activation capacity to directly activating secretion of immunomodulatory/pro-fibrotic molecules from human eosinophils, through TLR-2 and DP1 pathways. Of note, recent lipidome characterization of S. mansoni has demonstrated that egg-derived lipids from S. mansoni exhibit similar content of LPC and PGD 2 as the adult worm (48). Moreover, egg-derived lipids activate murine macrophages through TLR2-dependent pathways as observed for adult worms lipid extracts (3). As such, similar direct eosinophil activation is expect to occur with eggderived lipids, but further experiments will be necessary to fully address this question.
Regarding DP1 activation by PGD 2 on eosinophils, in addition to prior studies by our group that have shown its ability to promote lipid droplet-driven 5-LO-mediated LTC 4 synthesis, the current study is the first to show 15-LO activation by PGD 2 . Of note, besides eosinophils and virtually all other immune cells, DP1 is widely expressed on tissues, including the vasculature, the central nervous system, the retina, and the lungs (49)(50)(51)(52). Therefore, schistosomal PGD 2 has the potentiality to affect a variety of physiological functions in the host tissues. Even though PGD 2 has been initially perceived as a pro-inflammatory mediator, the exact function of DP1 activation has not been fully elucidated yet, but the understanding of PGD 2 /DP1 functions are now evolving to a more immunomodulatory/pro-resolving type similar to eosinophils themselves (53). For instance, by activating DP1 receptors, PGD 2 is known to inhibit the functions of platelet, neutrophils, basophils, and dendritic cells (54). In agreement, based on a study employing in vivo model of S. mansoni infection in DP1 deficient mice, authors have postulated that DP1 activation by S. mansoni-derived PGD 2 represent a schistosomal strategy to evade host immune defenses via altered balance of Th1/Th2 immune responses (55). In human eosinophils, activation of DP1 is not down-regulatory per se, however it appears to evoke primarily secretion of potential downmodulators. It is noteworthy that LPC and PGD 2 molecules are lipids that can also be produced by host cells during S. mansoni infection. For instance, we have shown that S. mansoni infection-derived stellate hepatic cells are able to synthesize LTC 4 and PGD 2 , which control in an autocrine fashion TGFβ release (40,56). Similarly, commercial LPC has been shown to modulate secretion of TGFβ by different cell types (4,57).
In conclusion, our findings provide original evidence that schistosomal lipids contain at least two components that are capable of direct activation of human eosinophils, LPC and PGD 2 that through receptor-mediated response trigger eosinophil activation characterized by rapid release of pro-inflammatory and pro-fibrotic mediators. The identification of bioactive schistosomal lipids and mapping the functional impact of these lipids on eosinophils are germane to better understanding the schistosomal pathology, as well as, may lead to new insights into improved treatment for both schistosomiasis and other eosinophilic immunological diseases.   Human eosinophils were stimulated with Pam3Cys (300 ng/ml), Schisto-TL (1 µg/mL) or Schisto-LPC (0.1 µg/mL) for 1 h. Primer sequences for human hTLR2 were used from TLR2 detection kit (Invivogen) and human GAPDH were used as housekeeping control.