Salmonella Effector SteA Suppresses Proinflammatory Responses of the Host by Interfering With IκB Degradation

Salmonella enterica serovar Typhimurium is known to cause its virulence by secreting various effector proteins directly into the host cytoplasm via two distinct type III secretion systems (T3SS-1 and T3SS-2). Generally, T3SS-1-delivered effectors help Salmonella Typhimurium in the early phases of infection including invasion and immune modulation of the host cells, whereas T3SS-2 effectors mainly help in the survival of Salmonella Typhimurium within the host cells including maintenance of Salmonella-containing vacuole, replication of the bacteria, and dissemination. Some of the effectors are secreted via both T3SS-1 and T3SS-2, suggesting their role in distinct phases of infection of host cells. SteA is such an effector that is secreted by both T3SS-1 and T3SS-2. It has been shown to control the membrane dynamics of the Salmonella-containing vacuole within the host cells in the late phases of infection. In this manuscript, toward characterizing the T3SS-1 function of SteA, we found that SteA suppresses inflammatory responses of the host by interfering with the nuclear factor kappa B pathway. Our initial observation showed that the mice infected with steA-deleted Salmonella Typhimurium (ΔsteA) died earlier compared to the wild-type bacteria due to heightened immune responses, which indicated that SteA might suppress immune responses. Furthermore, our study revealed that SteA suppresses immune responses in macrophages by interfering with the degradation of IκB, the inhibitor of nuclear factor kappa B. SteA suppresses the ubiquitination and hence degradation of IκB by acting on Cullin-1 of the Skp-1, Cullin-1, F-box (SCF)-E3 ligase complex. Our study revealed that SteA suppresses a key step necessary for E3 ligase activation, i.e., neddylation of Cullin-1 by interfering with dissociation of its inhibitor Cand-1.

Before the Salmonella enters into the host cell, the T3SS-1 is assembled by the bacteria across the host cell membrane. It generally translocates effectors, which are required for invasion and modulation of host immune responses. T3SS-1 effectors, such as SipA and SipC, bind to actin and help the bacteria in invasion (10)(11)(12); SopE and SopE2 modulate both actin rearrangement and host immune responses (13)(14)(15). Upon invasion, Salmonella-containing vacuoles (SCVs) are formed inside the host cell cytoplasm. Within the SCVs, bacteria replicate. T3SS-2 is formed across the SCV membrane, and the effectors are secreted into the host cell cytoplasm. T3SS-2 effectors are majorly required for maintenance of SCV and replication and spread of the bacteria (4,7). For example, SifA helps in the formation of Salmonella-induced filaments (SIF) (16)(17)(18). SIFs connect the SCV to various organelles like ER, Golgi, etc. of the cell, thereby expanding the replicative niche and acquiring nutrients for the replication of Salmonella (19)(20)(21).
In addition, some of the effectors such as SpvD, SlrP, SteE, SteB, GtgE, etc. are regulated by both SPI-1 and SPI-2 and translocated by both T3SS-1 and T3SS-2, indicating their role in both early and later phases of infection (22,23). SteA is one such effector molecule (24). Using Nramp1 mice, Lawley et al. have shown that SteA might play a role in the replication of Salmonella (25). Geddes et al. have reported that SteA could localize in the trans-Golgi network (22). However, Van Engelenburg and Palmer have later indicated that SteA could localize in Salmonella-induced tubules enriched with the trans-Golgi protein GalT (26). Furthermore, Domingues et al. have reported that, in the later phases of infection, SteA binds to phosphatidylinositol 4-phosphate [PI(4)P] to control membrane dynamics of SCV, thereby helping Salmonella to localize within SCV and in Salmonella-induced tubules (27,28). Matsuda et al. have indicated that SteA could induce T3SS-1-independent inflammation and cytotoxicity in macrophages (29). McQuate et al. have reported that SteA is important for establishing infection in the early phase and in the prevention of the clearance of Salmonella from macrophages (30). However, the SPI-1-regulated role of SteA in Salmonella infection is yet to be fully established.
In this study, toward unraveling the T3SS-1 role of SteA, we have shown that SteA suppresses the proinflammatory responses induced due to Salmonella Typhimurium infection. We observed that SteA interferes with the activation of nuclear factor kappa B (NF-κB), which is one of the major transcription factors known to be involved in the generation of proinflammatory responses. NF-κB in its inactive state remains in the cytoplasm bound with its inhibitor IκB. IκB degradation is necessary for NF-κB activation and is mediated by its ubiquitination. The E3 ligase is responsible for the ubiquitination of IκB (31). Our study revealed that SteA inhibits activation of Cullin-1, a component of E3 ligase complex and hence ubiquitination and degradation of IκB.
Abbreviations: steA, deletion mutant of steA gene; compl, steA complemented in the steA background; compl-H, 6X His-tagged steA complemented in the steA background.

Ethics Statement
All animal experiments were carried out in accordance with the guidelines of the

Bacterial Strains
S. enterica serovar Typhimurium SL1344 strain was a kind gift from Dr. Mahak Sharma (IISER Mohali). SteA in the genome of Salmonella Typhimurium was replaced with a Kanamycin cassette by one-step inactivation method following the protocol by Datsenko and Warner (32). Briefly, Kanamycin cassette was amplified from the plasmid pKD13 (a kind gift from Dr. Rachna Chaba, IISER Mohali) using primers-SteA H1P2 and SteA H2P1 ( Table 1). The amplified Kanamycin cassette with flanking regions corresponding to the flanking regions of the steA gene in the Salmonella Typhimurium genome was transformed into Salmonella Typhimurium expressing the λ-red recombinase via the helper plasmid pKD46 (a kind gift from Dr. Rachna Chaba, IISER Mohali). The colonies were selected on Kanamycin plates and screened by colony PCR. The deletion mutant of steA was then transduced to a clean background (Salmonella Typhimurium SL1344) using P22 phage (a kind gift from Dr. Rachna Chaba, IISER Mohali). For complementation, steA gene of Salmonella Typhimurium (www.ncbi.nlm.gov.in) was cloned in pACYC177 (a kind gift from Dr. Rachna Chaba, IISER Mohali) using restriction cloning and was transformed in the deletion mutant of SteA ( steA) background. The spi2 Salmonella Typhimurium 14028 (SV6017) strain was a kind gift from Dr. Francisco Ramos-Morales (University of Seville, Spain) (33). The P22 phage transduction method was used to construct spi2 SL1344 and spi2 steA SL1344 strains. The primers used in this study are listed in Table 1, and the strains and plasmids used in this study are listed in Table 2.

Infection of Cells
For inducing SPI-1 conditions, as described previously by Cardenal-Munoz and Ramos-Morales (24), bacteria were grown to stationary phase in Luria-Bertani (LB) broth supplemented with 0.3 M NaCl and appropriate antibiotics. Cells were plated in 24-or 6-well plates at a density of 1 × 10 6 cells/ml for overnight and infected with the stationary phase bacteria at a multiplicity of infection (MOI) of 10:1 (for BMDMs) or 20:1 (for RAW 264.7 and HEK 293 cells) for 30 min. The media containing the bacteria was removed, and fresh media supplemented with 100 µg/ml of gentamicin were added to the wells. After 1 h, this media was replaced with fresh media supplemented with 20 µg/ml of gentamicin for different time points depending on the assay. In all the experiments, the invasion was checked after 2 h of infection by enumerating the bacteria after the cells were lysed with 0.1% (v/v) Triton-X-100 (Himedia, India) in PBS.

Cell Cytotoxicity Assay
RAW 264.7 and BMDMs were plated at a density of 1 × 10 6 cells/ml and infected with wild type (wt), steA, or compl strains at an MOI of 20:1 or 10:1, respectively. After 8 h of infection, supernatants were collected, and cell cytotoxicity was checked using lactate dehydrogenase release assay (Promega, USA), according to the manufacturer's protocol.

Mice Infection and Scoring
Bacteria grown overnight in LB medium supplemented with 50 µg/ml of streptomycin were subcultured and grown until log phase at 37 • C. Balb/c mice 6-8 weeks old were infected intraperitoneally with 5 × 10 5 or 5 × 10 7 log-phase bacteria, and the survival of mice was monitored for 24 and 96 h, respectively. p values were calculated using Kaplan-Meir test using the software R. In addition, 36 h postinfection (hpi), the mice infected with 5 × 10 5 bacteria were scored for their response to stimuli, the extent of decrease in activity, the extent of eye closure and the pilorected fur as compared to the uninfected mice as described previously by Shrum et al. (34). In addition, the core body temperature of mice was checked using a noncontact infrared thermometer at 24 h after infection with 5 × 10 5 bacteria. The body temperature of mice was also checked before infection, and the difference in temperature of each mice was calculated. The blood of infected mice was collected at 24 and 36 h after infection, and serum was isolated from it. The serum was analyzed for tumor necrosis factor alpha (TNFα), interleukin (IL)-6, IL-1β, interferon gamma (IFN-γ), IL-12, and IL-10 (BD Biosciences, USA) using ELISA according to manufacturer's protocol.

Colonization and Splenic Lysate Preparation
Balb/c mice 6-8 weeks old were infected intraperitoneally with 5 × 10 5 log-phase bacteria. At 36 hpi, spleens were isolated and homogenized in PBS using a sterile pestle. For colonization, the homogenate was subjected to lysis with 0.1% (v/v) Triton-X-100 at 37 • C for 30 min. The bacteria were enumerated after serial dilution on LB agar (LA) plates supplemented with 50 µg/ml of streptomycin.
For splenic lysate preparation, the homogenate was pelleted by centrifugation at 2,000×g for 5 min and washed twice with PBS. Then, the pellet was treated with 500 µl of ACK lysis buffer [154.95 mM NH 4 Cl, 10 mM KHCO 3 , and 0.1 mM ethylenediaminetetraacetic acid (EDTA); pH 7.2] at room temperature for 5 min. To stop the reaction, 100 µl of FBS was added to it and was centrifuged at 2,000×g for 5 min and washed twice with PBS. The pellet was then resuspended in 70 µl of RIPA buffer [50 mM Tris-Cl (pH 8), 150 mM NaCl, 5 mM EDTA, 1% (v/v) NP-40, 0.5% (w/v) sodium deoxycholate, and 0.1% (w/v) sodium dodecyl sulfate] containing 1× mammalian protease inhibitor cocktail (Sigma-Aldrich, USA) and sonicated at 10 Å for three pulses of 5 s each. This was then centrifuged at 24,000×g for 30 min. The supernatant thus obtained was the splenic lysate.

TNFα and IL-6 Gene Expression
Balb/c mice 6-8 weeks old were infected intraperitoneally with 5 × 10 5 log-phase bacteria. At 36 hpi, spleens were isolated and were subjected to ballooning with 400 U/ml Collagenase D in HBSS and incubated at 37 • C for 25 min. To stop the reaction, 100 µl of 0.5 M EDTA was added and incubated at 37 • C for 5 min. This was then passed through a 40-µm strainer, and 5 ml of ice-cold RPMI 1640 media supplemented with 10% (v/v) FBS was added to it. The cells were then harvested at 2,000×g for 5 min and washed with PBS. The pellet was resuspended in 3 ml of 30% bovine serum albumin (Sigma-Aldrich, USA). To this, 1 ml of PBS was added slowly along the wall of the conical tube to form a layer and was subjected to density gradient centrifugation (with zero acceleration or deceleration) at 2,200×g for 30 min at 12 • C. The interface of the two layers contained the mononuclear cells, which were then harvested, and RNA was isolated according to the manufacturer's protocol using the Nucleopore RNA isolation Kit (Genetix, India). The complementary DNA (cDNA) was then synthesized using Verso-cDNA kit (Thermo-Fisher, USA). The PCRs were performed using Maxima SYBR green qPCR master mix (Thermo-Fisher, USA) and the Eppendorf Realplex master cycler. Primer sequences were used from the Harvard primer bank and were synthesized by Integrated DNA Technologies, USA.

Quantification of TNFα
RAW 264.7 and BMDMs were plated at a density of 1 × 10 6 cells/ml and infected with wt, steA, or compl strains at an MOI of 20:1 or 10:1, respectively. The supernatant was collected after 8 h of infection, and TNFα was quantified using ELISA (BD Biosciences, USA) as per the manufacturer's protocol. Furthermore, to confirm equal invasion in all the experiments, cells were lysed after 2 h of infection with 0.1% (v/v) Triton-X-100 at 37 • C for 30 min, and bacteria were enumerated on LA plates.

Transfection of HEK 293 Cells
HEK 293 (a human kidney epithelial cell line) cells (7 × 10 6 ) were plated in DMEM in a 90-mm Petri dish for wholecell lysate preparation, and 2.5 × 10 4 HEK 293 cells were seeded on coverslips in a 24-well plate for colocalization studies. Cells were then transfected with 3 µg (for wholecell lysates) or 1 µg (for colocalization) of pCDNA3.1(+) or pCDNA3.1(+)steA ( Table 2) using polyethyleneimine at a ratio of 1:3 (DNA/polyethyleneimine). The media was changed after 8 h of transfection. Then, after 24 or 36 h of transfection, the cells were stimulated with 10 ng/ml of TNFα for 15 or 30 min for whole-cell lysate preparation and 30 min for colocalization studies.

Luciferase Reporter Assay
For HEK 293 Cells HEK 293 cells (2.5 × 10 4 ) were plated in 100 µl of DMEM in a 96-well plate and were transfected with 0.1 µg each of NF-κB reporter plasmid pGL4.1 (Promega, USA) and renilla luciferase plasmid (pRL) ( Table 2) using Lipofectamine 3000 (Promega, USA) as per the manufacturer's protocol. In addition, in assays for studying the effect of endogenous expression of SteA on NF-κB activation, cells were also transfected with pcDNA3.1(+) or pcDNA3.1(+)steA in addition to pGL4.32 and pRL. After 18 h of transfection, the cells were either infected with wt, steA, and compl or stimulated with 10 ng/ml of TNFα for 6 and 8 h, respectively.
For RAW 264.7 Cells RAW 264.7 cells (5 × 10 4 ) were plated in 100 µl of RPMI in a 96-well plate and were transfected with 0.15 µg each of NF-κB reporter plasmid pGL4.1 (Promega, USA) and pRL ( Table 2) using FuGene HD (Promega, USA) as per the manufacturer's protocol. After 12 h of transfection, the cells were infected with wt, steA, and compl or stimulated with 500 ng/ml of LPS for 15 h.
The dual luciferase assay kit (Promega, USA) was used according to the manufactures' protocol to analyze the luminescence corresponding to NF-κB activation (firefly luciferase) and the renilla luciferase. The luminescence was detected using a plate reader (BMG Biotech, Germany). The luminescence corresponding to NF-κB activation was normalized to that of renilla luciferase (corresponding to the transfection efficiency). In infection-based experiments, the luminescence was also normalized to the invasion.

Colocalization Studies
After 24 h of transfection in HEK 293 cells and TNFα stimulation for 30 min (as described previously), cells were washed twice with PBS and were then fixed with 2.5% (w/v) of paraformaldehyde for 30 min at room temperature. Cells were then washed twice with PBS and incubated with anti-IκB (1:750) (Santa Cruz Biotechnologies, USA), anti-HA (1:250) (Biolegend, USA), anti-Cullin-1 (1:250) (Cell Signaling Technologies, USA), or anti-Cand-1 (1:500) (Cell Signaling Technologies, USA) antibodies for 45 min at room temperature. Then, cells were washed thrice with PBS and incubated with Alexa 488conjugated anti-rabbit immunoglobulin G secondary antibody (1:500) (Life Technologies, USA) and Alexa 568-conjugated anti-mouse immunoglobulin G secondary antibody (1:500) (Life Technologies, USA) for 30 min at room temperature. All the antibody dilutions were prepared in 0.2% (w/v) Saponin dissolved in PBS. The cells were washed thrice with PBS and were mounted on glass slides using Fluoromount (Sigma-Aldrich, USA). The imaging was performed using a Zeiss confocal microscope (for colocalization of IκB or Cullin-1 with SteA-HA) or Leica confocal microscope (for colocalization of Cand-1 with SteA-HA).

For Infection
Cells were plated at a density of 1.5 × 10 6 /ml in a six-well plate for overnight. Cells (4.5 × 10 6 ) (three wells of 1.5 × 10 6 /ml) were infected with wt, steA, or compl for 30 min. Then, the bacteria were removed, and RPMI 1640 media containing 100 µg/ml of gentamicin was added to each well for 30 min. The cells were then washed twice with sterile PBS and were then harvested at 2,000×g for 5 min. The pellet was then resuspended in 70-100 µl of whole-cell lysis buffer [50 mM Tris-Cl, 150 mM NaCl, 0.1% (w/v) sodium dodecyl sulfate (SDS) and 0.1% (v/v) Triton-X-100, pH 8] with mammalian protease inhibitor cocktail (Sigma-Aldrich, USA) and subjected to sonication at 10 Å for 15 s with a pulse of 5 s each. Then, it was centrifuged at 16,000×g for 30 min at 4 • C. The supernatant thus obtained was the whole-cell lysate.
In addition, the invasion was also checked by colony-forming unit counting for all the experiments.

For Transfections
After 24 h of transfection in HEK 293 cells (as described previously), cells were washed twice with PBS and were harvested by centrifugation at 2,000×g for 5 min. The pellet was then resuspended in 150-200 µl of whole-cell lysis buffer supplemented with mammalian protease inhibitor cocktail. This was then subjected to sonication at 10 Å for 15 s with a pulse of 5 s each. Then, it was centrifuged at 16,000×g for 30 min at 4 • C, and the supernatant obtained was collected as whole-cell lysate.

Nuclear Lysate Preparation
Cells (4.5 × 10 6 ) (three wells of 1.5 × 10 6 /ml) of RAW 264.7 were plated in a six-well plate overnight for each sample and then infected with wt, steA, or compl for 30 min. Then, the bacteria were removed, and RPMI 1640 media containing 100 µg/ml of gentamicin were added to each well for 30 min. Cells were then washed twice with sterile PBS and were then harvested at 2,000×g for 5 min. The pellet volume was measured, and it was dissolved in five times the pellet volume in hypotonic buffer (10 mM HEPES pH 7.9 with 1.5 mM MgCl 2 and 10 mM KCl) and centrifuged at 1,850×g for 5 min at 4 • C. Then, the pellet was dissolved in hypotonic buffer with 0.5 M dithiothreitol and mammalian protease inhibitor and was then incubated on ice for 15 min. This was then sonicated at 10 Å for 15 s with three pulses of 5 s each and centrifuged at 3,300×g for 15 min at 4 • C. The pellet was then resuspended in 70 µl of low salt buffer [20 mM HEPES pH 7.9, 1.5 mM MgCl 2 , 20 mM KCl, 0.2 mM EDTA, and 25% (v/v) glycerol] with 0.5 M DTT and mammalian protease inhibitor. To this, 30 µl of high salt buffer [20 mM HEPES pH 7.9, 1.5 mM MgCl 2 , 800 mM KCl, 0.2 mM EDTA, and 25 % (v/v) glycerol] was added dropwise and incubated on ice for 10 min before subjecting to sonication at 10 Å for 15 s. Then, it was incubated on ice for 30 min with periodic shaking and centrifuged at 24,000×g for 30 min at 4 • C. The supernatant hence obtained contained the nuclear lysate.
The horseradish-peroxidase-tagged secondary antibodies and antibody against glutathione-S-transferase (GST) were purchased from Sigma-Aldrich, USA. The immunoblots were developed using Clarity TM ECL Substrate (Bio-Rad, USA) and detected using LAS 4000 (GE Healthcare Technologies, USA).

Densitometric Analysis
The densitometric analysis of the immunoblots was carried out using the Image J software. The band intensities of IκB, IKKα/β, p65, or c-Rel were normalized to the respective band intensities of the loading controls, i.e., glyceraldehyde 3phosphate dehydrogenase for whole-cell lysates and PCNA for nuclear lysates. The band intensities of phosphorylated p38 and JNK were normalized to the band intensities of the total p38 and JNK. The lane intensities of ubiquitination and the band intensities of Cand-1 were normalized to the band intensities of IκB, and the band intensities of neddylated Cullin was normalized to the band intensities of Cullin-1.

Coimmunoprecipitation Studies
Whole-cell lysates were prepared (as previously described) and were incubated with 1-2 µg of anti-HA, anti-His, anti-Cullin-1, or anti-IκB antibody for 3-4 h with continuous low-speed shaking at 4 • C. Then, 20 µl of Protein A/G beads (Santa Cruz Biotechnologies, USA) was added, and it was subjected to continuous low-speed shaking at 4 • C for overnight. Then, the beads were washed thrice with whole-cell lysis buffer by centrifugation at 6,000×g for 5 min at 4 • C. The beads were resuspended in SDS-loading buffer and boiled for 10 min. The samples were then run on SDS-PAGE gel and subjected to immunoblotting.

For IκB Pulldown
HEK 293 cells were transfected with 3 µg of pcDNA3.1(+) or pcDNA3.1(+)steA (as described previously). After 24 h of transfection, cells were treated with 20 nM of proteasomal inhibitor, MG132 (Sigma-Aldrich, USA), for 3 h. The cells were then stimulated with 10 ng/ml of TNFα for 20 min, and wholecell lysates were prepared. The whole-cell lysates were then subjected to coimmunoprecipitation with 2 µg of the anti-IκB antibody as described above.

GST Pulldown Assay
The steA gene was cloned in the plasmid pGEX4T3 ( Table 2) using restriction cloning. The empty pGEX4T3 and pGEX4T3-steA were each transformed to Escherichia coli Origami B1 cells (Merck, Germany) by chemical transformation method. Then, using these transformants, GST and GSTtagged SteA were overexpressed using 0.1 mM isopropyl β-D-1-thiogalactopyranoside at 18 • C for 24 h. The bacteria were pelleted at 2,050×g for 30 min at 4 • C. The pellet was resuspended in PBS containing bacterial protease inhibitor cocktail (Sigma-Aldrich, USA) and was subjected to sonication at 25 Å for 15 min. Then, this was centrifuged at 18,500×g for 50 min at 4 • C and analyzed by SDS-PAGE. Both GST and GST-tagged SteA were found to be present in the soluble fractions. Parallelly, the glutathione beads (Qiagen, USA) were washed twice with lysis buffer [50 mM Tris-Cl, 150 mM NaCl, 0.05% (v/v) NP-40, pH 7.5]. Then, to 10 ml of the soluble fraction containing GST or GST-tagged SteA, 300 µl of washed glutathione beads were added and incubated at 4 • C with shaking. After 2 h of incubation, the beads were pelleted at 1,800×g for 5 min and washed four times with lysis buffer. Then, the beads were incubated with 500 µl of 5% (w/v) BSA in PBS for 90 min. Then, the beads were pelleted and washed four times with lysis buffer. To this, whole-cell lysate of unstimulated RAW 264.7 cells was added and incubated at 4 • C for 3-4 h with shaking. Then, the beads were pelleted and washed four times with lysis buffer and resuspended in 40 µl of SDS-loading buffer. This was then boiled for 5 min and subjected to SDS-PAGE and immunoblotting.

Yeast Two-Hybrid Assay
The coding sequences of IκB, Skp-1, Rbx-1, and Cullin-1 from Mus musculus were taken from www.ncbi.nlm.gov.in. A cDNA library was prepared according to manufacturer's protocol (Invitrogen, Life Technologies, USA) using RNA isolated (according to the manufacturer's protocol; Genetix, India) from untreated RAW 264.7 cells. IκB, Skp-1, and Rbx-1 were amplified from the cDNA library and cloned in pGADC1 ( Table 2) plasmid by restriction cloning. Cullin-1 was amplified and cloned in pGADT7 ( Table 2) by gateway cloning method according to manufacturer's protocol (Thermo-Fisher, USA). The steA gene was amplified from the Salmonella Typhimurium genome and cloned in pGBDC1 plasmid ( Table 2) by restriction cloning. The cloned AD and the BD plasmids were cotransformed in yeast (PJ697a) and plated on synthetic complete media deficient in leucine and uracil (SC-Leu, SC-Ura). The colonies thus obtained were then spotted on plates with media deficient in leucine, uracil, and histidine (SC-Leu, SC-Ura, SC-His).

Statistical Analysis
Data were expressed as mean ± standard error of mean (SEM), and p values were calculated using Student's two-sided t test or by one-way ANOVA followed by Tukey's multiple comparisons test. Significance is indicated as follows: * p < 0.05, * * p < 0.01, * * * p < 0.001, and nonsignificant (ns) when p > 0.05.

SteA Affects the Pathogenesis of Salmonella Typhimurium
To understand the effect of SteA deletion in mouse model of Salmonella infection, we constructed steA deletion mutant ( steA) of Salmonella Typhimurium and steA-complemented strain (compl) with steA-containing plasmid on steA background. Furthermore, we infected Balb/c mice with 5 × 10 5 and 5 × 10 7 wt, steA, and compl Salmonella Typhimurium. We have observed that the mice infected with steA died earlier than the wt and compl-infected mice (Figures 1A,  B). Furthermore, we have observed that steA-infected mice showed symptoms, namely, pilorected fur, decrease in activity, decrease in response to stimulus, and the extent of closing of the eyes that were graded on a scale of 1-4 (34) higher as compared to the control mice ( Figure 1C). The scoring indicated that the mice infected with the steA strain probably died of a heightened immune response. We also checked the body temperatures of mice infected with 5 × 10 5 wt, steA, and compl and found lower body temperatures of steAinfected mice, indicating that probably mice were undergoing sepsis (35).
Furthermore, to probe whether there was any increase in the proinflammatory cytokine levels in steA-infected mice compared to the controls, we checked TNFα, IL-6, IL-1β, IFN-γ, IL-12, and IL-10 in the serum of the infected mice and found higher TNFα, IL-6, IFN-γ, IL-12, and IL-10 level in the serum of the steA-infected compared to wt-or compl-infected mice ( Figure S1). Furthermore, we have isolated mononuclear cells from the spleen of mice infected with wt, steA, and compl strains and analyzed the expression of TNFα and IL-6 genes. We have observed higher expression of both TNFα and IL-6 genes in cells isolated from mice infected with the steA strains as compared to the wt-and compl-infected mice ( Figure 1E). Therefore, in accordance with our hypothesis, it seemed that there was an elevated inflammatory immune response in steA-infected mice compared to the controls. Furthermore, to check if the difference in immune response was due to a difference in the colonization pattern of steA compared to the wt strain, we have isolated spleen and enumerated the bacteria by colony-forming unit counting. No difference in the colonization of Salmonella Typhimurium was observed in the spleen of these respective strain-infected mice ( Figure 1F). Altogether, these results suggested that during Salmonella Typhimurium infection, absence of SteA enhances the host-inflammatory responses.

Increased TNFα Production in Macrophages Infected With steA
To further confirm that there is heightened inflammatory response upon infection with steA, we infected RAW 264.7 (murine macrophage cell line) and BMDMs with wt, steA, and compl strains. After 8 h of infection, supernatants were collected and analyzed for TNFα. In both the cell types, a higher TNFα production was observed when infected with steA compared to the wt and the compl-infected cells (Figure 2A). We further checked the cell cytotoxicity upon infection with wt, steA, and compl strains to probe whether this difference in TNFα production was due to a difference in cell death. However, we did not find any difference in cell cytotoxicity in RAW 264.7 or BMDMs infected with wt, steA or compl ( Figure S2). Therefore, our data confirmed that the Salmonella Typhimurium-mediated host-immune responses increase in the absence of SteA.
SteA is secreted by both T3SS-1 and T3SS-2. In addition, recently, it was reported that SteA could be secreted by T3SS-2 as early as 15 min in RAW 264.7 cells (24). Therefore, we wanted to check whether the observed effect of SteA was due to T3SS-2 effector function. To address this, we have used a spi2 mutant of Salmonella Typhimurium and a steA mutant . For each experiment, the average of total scores for three or four mice infected with either wt, steA, or compl was calculated. Bar graph represents mean ± SEM from three independent experiments. p values were calculated using one-way ANOVA of the total scores from three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. wt-infected mice). (D) Body temperatures of Balb/c mice infected with steA were lower than wt-or compl-infected mice. Balb/c mice 6-8 weeks old were infected intraperitoneally with 5 × 10 5 bacteria, and the body temperatures of mice were recorded before infection and at 24 hpi. Then, the difference in body temperature was calculated ( Temperature). Bar graph represents mean ± SEM from eight mice in each group. p values were calculated using one-way ANOVA of Temperature of eight mice from each group (n = 8) (*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. wt-infected mice). (E) Tumor necrosis factor alpha (TNFα) and interleukin (IL)-6 gene expression was higher in mononuclear cells isolated from spleen of mice infected with steA compared to those infected with wt or compl. The spleens of Balb/c mice infected with 5 × 10 5 bacteria were isolated at 36 hpi. The mononuclear cells were isolated using density-dependent separation [with bovine serum albumin (BSA)]. RNA was isolated from these cells; then, complementary DNA (cDNA) was prepared and gene expression of TNFα and IL-6 was analyzed by semiquantitative PCR. Fold change in gene expressions was calculated with respect to the gene expression in cells from mice infected with wt. Bar graph represents mean ± SEM from three independent experiments. p values were calculated using one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. wt-infected mice). (F) No significant difference in the colonization of wt and steA Salmonella Typhimurium in spleen of infected mice. Bacteria were enumerated in spleen of Balb/c mice infected intraperitoneally with 5 × 10 5 bacteria at 36 hpi. Graph represents colony-forming units (CFU) enumerated per milligram of spleen tissue from nine mice each (represented as a dot •). p values were calculated using Student's t test (*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. wt-infected mice). in spi2 background ( spi2 steA). We have treated BMDMs with wt, steA, spi2, and spi2 steA strains. After 8 h, we collected the supernatants and analyzed for TNFα by ELISA. The cells infected with spi2 steA showed higher TNFα production than spi2-infected cells in BMDMs (Figure 2B), indicating that SteA-mediated effect on the immune response is not T3SS-2 dependent.

SteA Acts on the NF-κB Pathway to Suppress the Immune Responses
An immune response involves the activation of majorly two transcription factors NF-κB and AP-1. The phosphorylation of mitogen-activated protein (MAP) kinases p38 and/or JNK majorly leads to the activation of AP-1 (36). To check whether MAP kinases could be involved in steAmediated heightened immune response, we infected RAW 264.7 cells with wt, steA, and compl strains and checked whether there is an increase in the phosphorylation levels of p38 and JNK MAP kinases in the whole-cell lysates. No difference in the phosphorylation level of p38 and JNK was observed in any of the treatments (Figures 3A,B). As phosphorylation levels are indicative of activation levels, our study indicated that SteA probably does not interfere in the MAP kinase activation pathway. As activation of the AP-1 transcription factor is dependent on MAP kinase activation, we presumed that probably SteA-mediated effect is not via the AP-1 pathway.
Furthermore, we wanted to check whether SteA has any effect on the activation of NF-κB. In an inactivated state, NF-κB remains bound to its inhibitor IκB, which prevents the translocation of NF-κB to the nucleus. Upon phosphorylation of IκB by upstream kinase IKKα/β, IκB gets ubiquitinated and degraded through proteasome rendering NF-κB free to translocate to the nucleus (31,37). We observed that in steAinfected RAW 264.7 cells and BMDMs, there is increased IκB degradation compared to control infected cells (Figures 4A,  B). NF-κB is known to be activated in HEK 293 cells in response to TNFα. To confirm the observation that SteA affects the degradation of IκB, we endogenously expressed SteA in HEK 293 cells and probed the IκB levels upon stimulation Whole-cell lysates were probed for phosphorylated levels of p38 and JNK by Western blots and densitometric analysis along with total p38 and JNK levels. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. For densitometric analysis, fold change was calculated with respect to wt. Bar graphs represent mean ± SEM from three independent experiments. p values were calculated using one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. wt-infected cells). UT stands for untreated cells.
with TNFα. We observed increased IκB degradation in TNFαactivated cells transfected with the empty plasmid, but in cells expressing SteA, IκB degradation was found to be inhibited ( Figure 4C). Furthermore, we also observed less IκB in splenic lysates of mice infected with steA compared to the control ( Figure 4D).
More degradation of IκB suggests increased translocation of NF-κB to the nucleus. Among the NF-κB family members, p65 and/or c-Rel are involved in the transcription of proinflammatory genes (37). Thus, to probe if IκB degradation corresponds to the nuclear translocation of NF-κB, we infected RAW 264.7 cells with wt, steA, and compl strains and checked the levels of p65 and c-Rel in the nuclear lysates. We observed that the nuclear translocation of both p65 and c-Rel were more in cells infected with steA compared to the wt and the compl-infected cells (Figure 5A). This observation indicated that the NF-κB activation during Salmonella Typhimurium infection increases in the absence of SteA. Furthermore, to confirm that SteA suppresses NF-κB activation, we transfected HEK 293 and RAW 264.7 cells with NF-κB promoter-reporter plasmid and then infected with wt, steA, and compl strains. The cells infected with steA showed higher NF-κB reporter activity than wt and the compl strains in both HEK 293 and RAW 264.7 cells (Figure 5B). Furthermore, we wanted to know if endogenous expression of SteA could also suppress NF-κB activation. Toward this, we endogenously expressed SteA in HEK 293 cells and then transfected with the NF-κB promoter-reporter plasmid. We observed that upon stimulation with TNFα, there is diminished NF-κB reporter activity in endogenous SteA-expressing cells as compared to the cells transfected with empty plasmid (Figure 5C).
Altogether, these results confirm that SteA suppresses the immune responses by acting on the NF-κB pathway.

SteA Interferes With the IκB Ubiquitination
IκB phosphorylation by IKKα/β is a prerequisite for IκB degradation. In response to an infection, a signaling cascade leads to the phosphorylation of IKKα/β, which in turn phosphorylates IκB (36). Since we have observed that there is more IκB degradation in steA-infected cells compared to the controls (Figure 4), we wanted to check whether this was due to increased activation of IKKα/β. No change was observed in the phosphorylation levels of IKKα/β in both RAW 264.7 macrophages and BMDMs infected with wt, steA, and compl strains (Figure 6A). This result indicated that increased degradation of IκB is probably not due to increased activation of IKKα/β. Hence, SteA seems to act downstream to the activation of IKKα/β for suppression of IκB degradation.
The degradation of IκB requires its polyubiquitination. To probe if SteA was interfering with the polyubiquitination of IκB and subsequently its degradation, SteA-expressing HEK 293 cells were pretreated with a pharmacological inhibitor (MG132), (Continued) FIGURE 4 | For densitometric analysis, fold change was calculated with respect to wt. Bar graphs represent mean ± SEM from three independent experiments. p values were calculated using one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. wt-infected cells). (C) Endogenous expression of SteA suppresses the degradation of IκB in HEK 293 cells. HEK 293 cells were transfected with pcDNA3.1(+)steA or pcDNA3.1(+) empty plasmid. After 18 h of transfection, cells were stimulated with TNFα for 15 and 30 min. Whole-cell lysates were then prepared, and IκB levels were analyzed by Western blotting and densitometry. GAPDH was used as a loading control in all the experiments. UT stands for untreated cells. For densitometric analysis, fold change was calculated with respect to UT cells. Bar graphs represent mean ± SEM from three independent experiments. p values were calculated using Student's t test (*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. UT cells). (D) IκB levels were lower in splenic lysates of mice infected with steA as compared to the control-infected mice. Balb/c mice were infected with wt, steA, or compl, and spleen was isolated at 36 hpi. Splenic lysates were then prepared, and the levels of IκB were probed by Western blotting and densitometric analysis. GAPDH was used as a loading control in all the experiments. For densitometric analysis, fold change was calculated with respect to wt. Bar graphs represent mean ± SEM from three independent experiments. p values were calculated using Student's t test (*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. wt-infected mice).

FIGURE 5 | SteA suppresses the nuclear factor kappa B (NF-κB) activation. (A)
The translocation of NF-κB subunits (p65 and c-Rel) was more in cells infected with steA than the controls. Nuclear lysates were prepared following infection of RAW 264.7 macrophages with wt, steA, or compl for 30 min. p65 and c-Rel were probed by Western blotting and densitometry. Proliferating cell nuclear antigen (PCNA) and Lamin B1 were used as a loading control for nuclear fractions. In addition, GAPDH and LAMP-1 were used as non-nuclear markers to check the purity of the nuclear lysates. For densitometric analysis, fold change was calculated with respect to wt. (B) NF-κB reporter activity was higher upon infection of HEK 293 cells and RAW 264.7 cells with steA than the wt or the compl-infected cells. HEK 293 or RAW 264.7 cells were transfected with NF-κB luciferase reporter plasmid and pRL (renilla luciferase) plasmid. After 18 or 12 h of transfection, respectively, cells were infected with wt, steA, or compl at an multiplicity of infection (MOI) of 20:1 (HEK 293 cells) or 5:1 (RAW 264.7 cells), and the reporter activity was measured following 8 or 15 h of infection, respectively. Tumor necrosis factor alpha (TNFα) stimulated HEK 293 cells, and lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were taken as positive control for the experiment. (A,B) Bar graph represents mean ± SEM from three independent experiments. p values were calculated using one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. wt-infected cells). (C) Endogenous expression of SteA in HEK 293 cells suppresses the NF-κB reporter activity upon TNFα stimulation. HEK 293 cells were transfected with NF-κB Luciferase reporter plasmid, pRL plasmid, and pcDNA3.1(+)steA or pcDNA3.1(+) empty plasmid. After 18 h of transfection, cells were stimulated with TNFα, and the reporter activity was measured after 8 h. Bar graph represents mean ± SEM from three independent experiments. p values were calculated using Student's t test [*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. pcDNA3.1(+) transfected cells]. The levels of IKKα/β were probed by Western blotting and densitometry. For densitometric analysis, fold change was calculated with respect to wt. Bar graphs represent mean ± SEM from three independent experiments. p values were calculated using one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. wt-infected cells). (B) SteA suppresses the ubiquitination of IκB. HEK 293 cells were transfected with pcDNA3.1(+)steA or pcDNA3.1(+) empty plasmid. After 24 h of transfection, cells were pretreated with proteasomal inhibitor MG132 for 3 h and stimulated with TNFα for 20 min. Whole-cell lysates were then prepared and immunoprecipitated with anti-IκB antibody. Ubiquitination was then analyzed by Western blotting and densitometry. (A,B) Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. For densitometric analysis, fold change was calculated with respect to pcDNA3.1(+) transfected cells. Bar graphs represent mean ± SEM from three independent experiments. p values were calculated using Student's t test [*p < 0.05, **p < 0.01, ***p < 0.001, ns p > 0.05 vs. pcDNA3.1(+) transfected cells].
which prevents the proteasomal degradation of ubiquitinylated proteins and immunoprecipitated with anti-IκB antibody. We observed that the ubiquitination of IκB was less when SteA was present ( Figure 6B).
Altogether, these results showed that SteA interferes with the ubiquitination of IκB to suppress the host proinflammatory responses.

SteA Does Not Inhibit the Recruitment of E3 Ligase Components to IκB
For ubiquitination to happen, the phosphorylated IκB is recognized by the Skp-1, Cullin-1, F-box (SCF)-E3 ligase complex. Recruitment of the SCF complex to the phosphorylated IκB happens through F-box protein β-TrCP (31,38). Therefore, to probe whether SteA could affect the recruitment of the SCF complex to IκB, we immunoprecipitated the lysates of wt and steA-infected RAW 264.7 macrophages with anti-β-TrCP antibody and observed the association of SCF complex with IκB both in presence or absence of SteA. This showed that SteA was not interfering with the assembly of the SCF-E3 ligase complex to phosphorylated IκB (Figure 7A and Figure S3A).
Furthermore, to understand how SteA affects IκB ubiquitination, we checked whether SteA could associate with IκB. Toward this, we overexpressed SteA in HEK 293 cells and checked the localization of SteA with IκB upon stimulation with TNFα using confocal microscopy. We observed that IκB and SteA colocalize upon stimulation in cells overexpressing SteA (Figure 7B). Furthermore, to confirm the association of SteA with IκB and SCF complex, we have immunoprecipitated HA-tagged SteA-expressing HEK 293 cell lysates using anti-HA antibody following TNFα stimulation. We have observed that the members of the SCF complex and IκB coimmunoprecipitated with SteA ( Figure 7C and Figure S3B). Furthermore, upon immunoprecipitation of RAW 264.7 cells infected with steA and complemented strain expressing His-tagged SteA (compl-H) using anti-His antibody, we observed that SteA coimmunoprecipitated with the SCF complex as well as IκB (Figure 7D and Figure S3C) even upon infection.
These results indicated that SteA does not inhibit the binding of SCF complex to IκB, and SteA itself binds to the IκB-SCF complex.

SteA Binds to Cullin-1
To better understand how SteA suppresses IκB degradation, we wanted to explore with which component of the SCF complex SteA binds or whether it directly binds to IκB. Toward this, we did a yeast two-hybrid screen for the interaction of SteA with IκB and the members of the SCF complex ( Figure 8A). In this screen, we observed that SteA binds to Cullin-1 but not to IκB, Rbx-1, or Skp-1, indicating that SteA directly interacts with Cullin-1.
Interestingly, we also observed colocalization of SteA with Cullin-1 in both TNFα-stimulated and unstimulated SteA-transfected HEK 293 cells using confocal microscopy, suggesting an association of SteA with Cullin-1 even when SCF complex is not assembled at IκB (Figure S5A). Furthermore, we did a GST pulldown assay in whole-cell lysates of unstimulated RAW 264.7 cells using GST-tagged SteA and observed that Cullin-1 was coimmunoprecipitated with GST-tagged SteA (Figure 8B and Figure S4B). In addition, in HEK 293 cells expressing HA-tagged SteA, we observed that SteA coimmunoprecipitated with Cullin-1 upon immunoprecipitation with anti-Cullin-1 antibody (Figure 8C and Figure S4A).
Altogether, these data confirmed that SteA interacts with Cullin-1 to suppress IκB degradation.
SteA Binds to Cullin-1 and Prevents Its Neddylation by Interfering With the Dissociation of Cand-1 From Cullin-1 Generally, upon assembly of the SCF complex to IκB, Cullin-1 gets neddylated. This neddylation of Cullin-1 is a crucial step for the activation of the SCF-E3 ligase, which leads to the ubiquitination of IκB (39). Upon neddylation, Cand-1, which is bound to Cullin-1 in the inactivated state, is removed from the E3 ligase complex (40). Therefore, we first checked the neddylation of Cullin-1 in HEK 293 cells transfected with pcDNA3.1(+) and pcDNA3.1(+)steA and observed that, upon TNFα stimulation, Cullin-1 was less neddylated in cells expressing SteA ( Figure 9A). Furthermore, to check if Cand-1 dissociation from the E3 ligase complex was inhibited in the presence of SteA, we stimulated SteA-expressing HEK 293 cells after pretreatment with MG132 and immunoprecipitated with anti-IκB antibody and probed for Cand-1. We observed that Cand-1 dissociation was lesser in the presence of SteA (Figure 9B). In addition, in GST pulldown assay, we observed the association of Cand-1 to GST-tagged infection triggers a signaling cascade, which leads to the phosphorylation of IKKα/β. IKKα/β then phosphorylates IκB, which is the inhibitor of NF-κB. In the inactivated state, IκB is bound to NF-κB, thus preventing NF-κB to translocate to the nucleus. Phosphorylated IκB is recognized by the F-box protein β-TrCP, which further leads to recruitment of the Skp-1 and Cullin-1 (Cul-1) to form the SCF-E3 ligase complex. Cullin-1 is bound to Cand-1 in the inactive state, and upon neddylation, Cand-1 dissociates from Cullin-1 leading to the complete activation of the E3 ligase. The E3 ligase complex is responsible for the polyubiquitination of IκB. Upon polyubiquitination, IκB undergoes proteasomal degradation, rendering NF-κB free to translocate to the nucleus and transcribe proinflammatory genes. (B) During wild-type (wt) Salmonella Typhimurium infection (when SteA is present), SteA binds to Cullin-1 (of SCF-E3 ligase) and suppresses the neddylation of Cullin-1 and the dissociation of Cand-1 from Cullin-1, thereby suppressing the polyubiquitination of IκB. This leads to the suppression of NF-κB-mediated proinflammatory responses.
SteA even in unstimulated RAW 264.7 cells, indicating that SteA remains associated with Cullin-1/Cand-1 complex even without stimulation ( Figure 9C and Figure S4C). To further confirm this, we checked the colocalization of SteA and Cand-1 in SteAtransfected HEK 293 cells, and we observed no difference in the colocalization of SteA and Cand-1 with or without TNFα stimulation ( Figure S5B).
Altogether, this study shows that SteA suppresses the immune responses of the host by suppressing the degradation of IκB. SteA prevents the degradation of IκB by binding to Cullin-1 and thus suppressing the neddylation of Cullin-1, which is necessary for the complete activation of the E3 ligase complex to ubiquitinate IκB (Figure 10).

DISCUSSION
In response to a bacterial infection, the immune cells of the host elicit a proinflammatory response and signal other cells of the intrusion. This eventually leads to clearing of the bacteria from the host system. Some pathogens including Salmonella Typhimurium have been known to evade the immune system of the host and establish a niche in the macrophages. Salmonella Typhimurium makes use of some of the effectors that it translocates directly into the host cells via the type three secretion systems (T3SS) to suppress the host's immune responses. The effectors secreted by T3SSs, mainly T3SS-1, play different roles in immunomodulation of the host (8). Toward characterizing the T3SS-1 role of SteA, we found that mice infected with steA-deficient Salmonella Typhimurium ( steA) showed lower survival as compared to the wt Salmonella Typhimurium infected mice (Figures 1A,B). Furthermore, a higher immune response in mice infected with steA seemed to be the cause of increased lethality (Figures 1C-E and Figure S1). Similarly, higher proinflammatory responses were observed when RAW 264.7 murine macrophage cell line and BMDMs were infected with steA as compared to wt-infected cells (Figure 2A). Furthermore, we confirmed that SteA-mediated suppression of immune response is a T3SS-1 effect using a strain deficient in T3SS-2 ( spi2) and a spi2 steA double mutant ( Figure 2B).
The proinflammatory responses are mediated by a multistep signaling cascade involving the activation of the NF-κB pathway or the MAP kinase pathway leading to the activation of transcription factors NF-κB and AP-1 (36). Some of the effectors of Salmonella Typhimurium are already known to suppress the host-immune responses. For example, SpvC suppresses the MAP kinase pathway by dephosphorylating JNK and ERK (41), SptP acts on the ERK activation (42,43), while AvrA suppresses both the NF-κB and the MAP kinase pathway (44)(45)(46)(47), hence suppressing inflammation. To be able to understand how SteA suppresses the proinflammatory responses, we first checked the activation levels of p38 and JNK MAP kinases in the wholecell lysates and the levels of NF-κB family members (p65 and c-Rel) in the nuclear lysates. We observed that SteA does not affect the MAP-kinase activation but acts on the NF-κB pathway to suppress the immune responses (Figures 3-5). To confirm our observation, we transfected HEK 293 and RAW 264.7 cells with NF-κB luciferase reporter plasmid and found the activation of NF-κB to be higher upon infection with steA than the wt ( Figure 5B). Furthermore, upon endogenous expression of SteA in HEK 293 cells, the NF-κB reporter activity was found to be suppressed ( Figure 5C). Since suppression of the NF-κB pathway is crucial for the survival of Salmonella Typhimurium in the host, Salmonella Typhimurium secretes various effectors to control it at multiple levels. In the inactive state, NF-κB remains bound to the inhibitor IκB; for the activation of NF-κB, IκB kinase (IKK) phosphorylates IκB and leads to its ubiquitination and degradation rendering NF-κB free. The free NF-κB transcription factor translocates to the nucleus, binds to the DNA, and upregulates transcription of inflammatory cytokine genes (37). Some effectors such as AvrA and SseL are known to interfere with IκB degradation by deubiquitinating IκB (44,48). Another effector, SpvD, affects the recycling of NF-κB, and PipA acts as a protease, which cleaves RelA, a subunit of NF-κB after its activation (49, 50). Toward exploring how SteA suppresses the NF-κB pathway, we checked the degradation level of IκB in the presence or absence of SteA and observed increased degradation of IκB in the absence of SteA compared to when SteA was present, suggesting that SteA acts on IκB degradation (Figure 4). The degradation of IκB is a multistep pathway which is initiated by the phosphorylation of IκB. IκB phosphorylation is dependent on IKK; therefore, there could be increased activation of IKK itself in the absence of SteA, but we observed comparable activation of IKK in cells infected with wt or steA strains ( Figure 6A). Following phosphorylation, the SCF-E3 ligase complex assembles on IκB, leading to the polyubiquitination of IκB and hence its proteasomal degradation (31). One of the effectors, GogB, suppresses ubiquitination by acting on Skp-1, a component of SCF-E3 ligase (51). Our study indicated that SteA also binds to the SCF complex and suppresses the activation of IκB by interfering with the ubiquitination (Figure 6B). However, we observed that SteA mainly acts on Cullin-1 of SCF-E3 ligase complex (Figure 8). For ubiquitination to happen, the neddylation of Cullin-1 follows the assembly of SCF-E3 ligase complex to IκB. Neddylation of Cullin-1 is a crucial step for the complete activation of the E3 ligase (39). Cullin-1 in the inactive form remains bound to Cand-1; however, upon activation, Cand-1 dissociates from Cullin-1 allowing neddylation to happen (40). Our study indicated that SteA binds to Cullin-1 (Figure 8), thus interfering with neddylation of Cullin-1 and the dissociation of Cand-1 from Cullin-1 (Figure 9). This further results in suppression of IκB ubiquitination and its degradation.
In response to Salmonella Typhimurium infection, proinflammatory responses are generated in the host. This immune response is capable of clearing the infection; also, if unchecked, it may be lethal for the host. However, for Salmonella Typhimurium to be able to survive and multiply in the host, these proinflammatory responses need to be suppressed. For this, Salmonella Typhimurium secretes an array of effectors which suppress the immune responses at multiple levels and ensures its survival in the host.
Altogether, our study shows that Salmonella Typhimurium effector SteA suppresses the proinflammatory responses generated against Salmonella Typhimurium by suppressing the neddylation of Cullin-1 and hence suppressing the degradation of IκB, eventually leading to the suppression of NF-κB activation (Figure 10).

DATA AVAILABILITY STATEMENT
All datasets generated for this study are included in the article/Supplementary Material. The raw datasets will be available on request.

ETHICS STATEMENT
The animal study was reviewed and approved by the Institutional Animals Ethics Committee (IAEC) of the Indian Institute of Science Education and Research, Mohali.