Eosinophils Control Liver Damage by Modulating Immune Responses Against Fasciola hepatica

Eosinophils are granulocytes that participate in the defense against helminth parasites and in hypersensitivity reactions. More recently, eosinophils were shown to have other immunomodulatory functions, such as tissue reparation, metabolism regulation, and suppression of Th1 and Th17 immune responses. In the context of parasitic helminth infections, eosinophils have a controversial role, as they can be beneficial or detrimental for the host. In this work, we investigate the role of eosinophils in an experimental infection in mice with the trematode parasite Fasciola hepatica, which causes substantial economical losses around the world due to the infection of livestock. We demonstrate that eosinophils are recruited to the peritoneal cavity and liver from F. hepatica-infected mice and this recruitment is associated with increased levels of CCL11, TSLP, and IL-5. Moreover, the characterization of peritoneal and hepatic eosinophils from F. hepatica-infected mice showed that they express distinctive molecules of activation and cell migration. Depletion of eosinophils with an anti-Siglec-F antibody provoked more severe clinical signs and increased liver damage than control animals which were accompanied by an increase in the production of IL-10 by hepatic and splenic CD4+ T cells. In addition, we also report that eosinophils participate in the modulation of humoral immune responses during F. hepatica infection, contributing to their degranulation. In conclusion, we demonstrate that eosinophils are beneficial for the host during F. hepatica infection, by limiting the production of IL-10 by specific CD4+ T cells and favoring eosinophil degranulation induced by specific antibodies. This work contributes to a better understanding of the role of eosinophils in parasitic helminth infections.


INTRODUCTION
Fasciola hepatica, a worldwide-distributed liver fluke, is one of the causative agents of fasciolosis, a zoonotic disease that affects livestock and humans (1). Indeed, the World Health Organization estimates that around 17 million people are infected and 180 million are at risk of infection, predominantly in South America and Africa (2). In addition, fasciolosis causes huge economical losses due to livestock infection, of approximately 3 billion dollars per year (1). The life cycle of F. hepatica is complex as the parasite goes through multiple stages before reaching its adult form and includes an intermediate host, a water snail of the Lymnaea genus, and a definitive host, usually livestock or humans (3). After ingestion of metacercariae by the mammalian host, juvenile flukes penetrate the host's intestine wall and reach the liver between 4 and 6 days. Eventually, the flukes reach the bile ducts, where they become sexually mature (3). F. hepatica causes chronic infections due to sophisticated immune modulation strategies that make possible its long-period survival in the host. Indeed, several studies have shown that the parasite induces regulatory dendritic cells (4,5), alternative activated macrophages (6), and a type 2 modified immune response characterized by an important regulatory T cell (Treg) component (4,7).
Eosinophils, granulocytes belonging to the innate immune system, participate mainly in the defense against multicellular parasites and in several Th2-driven immune disorders, such as asthma, atopic dermatitis, and eosinophilic esophagitis (8). The classical functions of eosinophils include mainly degranulation triggered by antibodies in a mechanism known as antibodydependent cell cytotoxicity (ADCC) (8,9). However, in the last years, novel immunomodulatory functions have been reported such as the regulation of glucose metabolism in the adipose tissue (10), enhancement of plasma cells survival (11), and suppression of Th1 immune responses (12,13).
Eosinophils play a pivotal role in fighting against some helminth infections (9), reflected by their dramatic increase in response to IL-5 produced by CD4 + type 2 helper T cells (Th2) (14). Although their capacity for killing helminths through ADCC in vitro has been well demonstrated (15, 16), their role in vivo remains controversial. While in some cases eosinophils appear to be beneficial for the host (17) in others their presence is redundant (18) or even of apparent benefit for the parasite (19). In F. hepatica infection, however, the role of eosinophils remains unknown.
In this study we characterized eosinophils during experimental infection with F. hepatica in mice and demonstrate that they contribute to limit liver damage induced by the parasite by reducing the production of IL-10 by CD4 + T cells. In addition, our results indicate that eosinophils play a role in specific humoral immunity by inducing the production of more effective antibodies in triggering eosinophil degranulation. Thus, the present study contributes to the elucidation of immunomodulatory mechanisms mediated by eosinophils during F. hepatica infection and collaborates with the understanding of their role in helminth infections.

Ethics Statement
Mouse experiments were carried out in accordance with strict guidelines from the National Committee on Animal Research (Comisión Nacional de Experimentación Animal, CNEA, http:// www.cnea.gub.uy/, National Law 18.611, Uruguay) according to the international statements on animal use in biomedical research from the Pan American Health Organization (PAHO) and World Health Organization (WHO). The protocol was approved by the Uruguayan Committee on Animal Research. Cattle's livers were collected during the routine work of a local abattoir (Frigorífico Carrasco) in Montevideo (Uruguay).

Mice
Six-to eight-week-old female BALB/c mice were purchased from DILAVE Laboratories (Uruguay). Animals were kept in the animal house (URBE, Facultad de Medicina, UdelaR, Uruguay) with water and food supplied ad libitum. Mouse handling and experiments were carried out in accordance with strict guidelines from the National Committee of Animal Research (CNEA, Uruguay). All procedures involving animals were approved by UdelaR's Committee on Animal Research (CHEA, Protocol Number: 070153-000820-17).

Preparation of Protein Lysates From F. hepatica
Live adult worms of F. hepatica were obtained from the bile ducts of bovine livers, washed in phosphate-buffered saline (PBS) pH 7.4, then mechanically disrupted and sonicated. After centrifugation at 40,000 × g for 60 min, supernatants were collected and dialyzed against PBS. The obtained lysate (FhTE) was quantified and stored at -80 • C. The endotoxin levels were determined by using the Limulus Amebocyte Lysate kit Pyrochrome (Associates of Cape Cod). Adult worms were collected during the routine work of a local abattoir (Frigorífico Carrasco and Sarubbi) in Montevideo (Uruguay). Protocols were approved by the Uruguayan Committee on Animal Research (CHEA, Protocol Number: 070153-000820-17).

F. hepatica Infections
BALB/c mice were orally infected with 10 F. hepatica metacercariae (Montevideo, Uruguay) per animal. After 8, 15, or 21 days post-infection (d.p.i.) mice were bled and peritoneal exudate cells (PECs), spleens, and livers were removed. Noninfected animals were used as controls. At least 4 animals were used per group. Peritoneal exudate cells were harvested by washing the peritoneal cavity with 10 ml of cold PBS. For the isolation of hepatic leukocytes, a previously described protocol was followed (18). Briefly, livers were mechanically dissociated and the cell suspension was left on ice for 15-20 min. The supernatant was then recovered in a new tube and centrifuged at 120 × g for 7 min at 4 • C. The pellet was resuspended in 40% Percoll gradient and centrifuged at 600 × g for 20 min at 20 • C, after which the hepatic leukocytes were obtained. Finally, red cells were lysate and hepatic leukocytes were washed with PBS.
In order to deplete eosinophils, mice (n = 7-8/group) received an intraperitoneal injection of 15 µg of monoclonal rat IgG 2a anti-Siglec-F antibody (clone E50-2440 from BD Biosciences, United States), the day before and after infection with F. hepatica and every 3 days until sacrifice. The control group consisted of mice injected intraperitoneally with isotype-matched control antibody (BD Biosciences, United States). Mice were sacrificed at day 20 post-infection, bled, and PECs, spleens and livers were removed and used for the following experiments. The severity of the infection was assessed by determining the general state of the animal by a defined clinical score according to the following parameters: peritoneal hemorrhage, macroscopic liver damage, splenomegaly, and cell content in the peritoneal cavity (20), where the minimum score was 0 whereas maximal score was 10. Liver damage was quantified by testing alanine aminotransferase (ALT) activity in sera, determined by using a commercial kit (Spinreact, Spain) according to the manufacturers' instructions.

Sorting of Eosinophils
Peritoneal exudate cells from infected mice after 18-20 d.p.i. were obtained and stained with antibodies against Siglec-F and F4/80 for 30 min at 4 • C. Eosinophils (Siglec-F + F4/80 int ) were sorted at 4 • C in a BD FACSAria Fusion Cell Sorter. The purity and viability of the sorted eosinophils was >90%.

Histology
Livers from infected mice after 21 d.p.i. or non-infected mice (control) were harvested, embedded in Polyfreeze (Sigma-Aldrich, United States), and snap-frozen in nitrogen. Sections were cut at a thickness of 10 µm, stained with hematoxylin and eosin and analyzed under light microscope Zeiss Axio LabA1.

Immunofluorescence
Peritoneal exudate cells and sorted eosinophils from infected mice were dispersed in silanized slides. Cells were permeabilized with 0.1% Triton X-100 in PBS for 15 min and blocked with 5% BSA for 1 h at room temperature. Cells were then overnight incubated at 4 • C with anti-Siglec-F ( For May-Grünwald Giemsa staining, cells were stained with May-Grunwald staining for 1 min, washed with distilled water, followed by Giemsa staining. Finally, cells were washed with distilled water and analyzed in a microscopere Nikon Eclipse E400 and Zeiss Axio Lab.A1.

Determination of Cytokines and Chemokines
IFN-γ, IL-4, IL-5, IL-10, thymic stromal lymphopoietin (TSLP), IL-5, CCL11 levels in culture supernatants or ascites of infected and non-infected mice were quantified by interleukin-specific sandwich ELISA assays using the corresponding antibodies from BD Bioscience or Biolegend. TGFβ and FIZZ-1 were detected by real-time qRT-PCR using an Eco real-time PCR System (Illumina, United States) using Fast SYBR R Green Master Mix (Applied Biosystems, United States). Total RNA was isolated from BALB/c mice treated with α-Siglec-F antibody or with isotype control, and from non-infected mice by using RNeasy Mini Kit (Qiagen, United States). Standard amplification conditions were 10 min at 95 • C for initial activation, followed by 40 thermal cycles of 15 s at 95 • C, 30 s at 60 • C and 30 s at 72 • C with a final extension of 10 min at 72 • C. The following primers were used: TGFβ-F: 5 -AACAATTCCTGGCGTTACCTT-3 ; TGFβ-R: 5 -CTGCCGTACAACTCCAGTGA-3 ; GAPDH-F: 5 -CTGAGAACGGGAAGCTTG-3 ; GAPDH-R: 5 -CCTGCT TCACCACCTTCTTG-3 ; FIZZ1-F: 5 -CACCTCTTCACTCGA GGGACAGTTG-3 ; FIZZ1-R: 5 -GGTCCCAGTGCATATGG ATGAGAC-3 . Results were expressed as the ratio between each evaluated cytokine and GAPDH expression. Expression was calculated using the 2-CT method and normalized to GAPDH. All reactions were performed with at least five biological replicates.

Determination of Specific F. hepatica Antibodies
To determine parasite-specific antibody titers in sera an indirect ELISA assay was performed. Briefly, 96-well microtiter plates (Nunc, Denmark) were coated with FhTE (2 µg/well) in 50 mM carbonate buffer (pH 9.6). After blocking with 1% gelatin in PBS, three washes with PBS containing 0.1% Tween-20 were performed. Serially diluted sera in PBS containing 0.1% Tween-20 and 0.5% gelatin were added to the wells for 1 h at 37 • C. After three washes, wells were treated 1 h at 37 • C using goat anti-mouse IgM, IgA, IgG IgG 1 , IgG 2a , IgG 2b , or IgG 3 peroxidase-conjugates (Sigma-Aldrich, United States) followed by o-phenylenediamine (OPD) and H 2 O 2 as substrates. Plates were read photometrically at 492 nm in an ELISA auto-reader (Thermo Fisher Scientific). Antibody titers were calculated to be the log 10 highest dilution, which gave twice the absorbance of control (mock) mouse sera with the minor dilution. Titers are shown as the arithmetic mean ± SEM.

Differentiation of Bone Marrow-Derived Eosinophils
Eosinophils were generated from cultures of bone marrow cells from infected and non-infected mice based on a previously described protocol (20). Briefly, bone marrow from the tibia and femur of BALB/c infected mice was used at 18 d.p.i. Cells were cultured at 0.5 × 10 6 /mL in complete RPMI medium supplemented with 100 ng/mL SCF and 100 ng/mL FLT3-L (PeproTech). On day 4, the media containing SCF and FLT3-L was replaced with media containing 10 ng/mL IL-5 (PeproTech) alone and cultured for 10 days at 37 • C and 5% CO 2 .

Degranulation of Eosinophils
Eosinophil degranulation was determined through the measurement of the eosinophil peroxidase (EPO) enzyme liberation, as previously described (21). Briefly, bone marrowdifferentiated eosinophils were collected by centrifugation and resuspended in complete RPMI medium in a ninety-six-well microtiter culture plate (1 × 10 6 /well) in the presence or absence of sera from infected and non-infected mice (dilute 1/50) and FhTE (100 µg/mL) for 3 h at 37 • C. The assay was developed using 100 µL OPD reagent (800 µL 15 mM OPD in 4 mL 1 M Tris (pH 8.0), 9.2 mL H 2 O and 4 µL 30% H 2 O 2 ). The reaction was terminated by the addition of 100 µL of 3 M HCl to each well and read at 492 nm. Control conditions corresponded to untreated cells.

Statistics
Experiments were performed as indicated and expressed as mean ± SEM. Statistical analyses were performed using GraphPad Prism version 6.04 for Windows (GraphPad Software, United States). Results were analyzed using one-way ANOVA followed by Tukey's test, or two-tails student's t-test. Significant differences were considered as follows: * p < 0.05; * * p < 0.01; * * * p < 0.001; * * * * p < 0.0001.

Eosinophils Are Recruited to the Peritoneal Cavity and Liver During F. hepatica Infection
We first evaluated whether Siglec-F + cells were present in F. hepatica-infected animals. To this end, mice were infected with 10 metaceracariae and after 3 weeks post-infection (w.p.i.), livers, spleens and PECs were removed and eosinophils were analyzed by flow cytometry. These studies revealed that Siglec-F + cells expressed intermediate levels of F4/80, thus they were identified as Siglec-F + F4/80 int cells (Supplementary Figure 1A). These cells were detected in the peritoneal cavity, spleen and liver from infected animals. However, a recruitment of eosinophils both in PECs and liver was observed in F. hepatica-infected animals, while only a slight increase of these cells was found in the spleen ( Figure 1A). Microscopy examination of May-Grunwald Giemsa stained PECs from infected animals showed a heterogenic group of cells comprising large cytoplasmic granules that stain purple and bi-lobulated nuclei typical of mouse eosinophils and cells with macrophage morphological features ( Figure 1B, upper photo). Siglec-F + F4/80 int cells were then sorted and microscopic examination confirmed that these cells were eosinophils (Figure 1B, lower photo). Last, granulocyticlike cells were also detected in livers from infected animals by their purple staining present in the leukocyte infiltrate of livers ( Figure 1C).
Then, we analyzed the presence of eosinophils during the experimental infection with F. hepatica. To this end, infected mice were sacrificed at 8, 15, and 21 d.p.i. to study the presence of eosinophils during the course of the infection. The analyses of infected mice revealed a progressive increase in the clinical signs (score) caused by the infection since day 8 post-infection, while liver damage was only detected at 21 d.p.i. evaluated by the ALT activity in serum (Figure 2A), a common marker to detect hepatic dysfunction (21). An increase of Siglec-F + F4/80 int cells was detected in the peritoneal cavity of infected mice at 8 d.p.i. that was increased as infection progressed ( Figure 2B and Supplementary Figure 2A). On the other hand, hepatic Siglec-F + F4/80 int cells were detected only after 15 d.p.i. (Figure 2C and Supplementary Figure 2B) suggesting that the recruitment of eosinophils to the liver is a later event in the infection. Last, we analyzed the presence of important cytokines and chemokines for the recruitment or differentiation of eosinophils, such as CCL11, also known as eotaxin 1, TSLP and IL-5, both in the peritoneal cavity and serum. As shown in Figure 2D, TSLP, and IL-5 levels in the peritoneum increased at 8 d.p.i. while higher levels of CCL11 were found after 15 d.p.i. TSLP, CCL11, and IL-5 levels were highest at 21 d.p.i. On the other hand, non-detectable levels of these cytokines and chemokine were detected in sera from infected animals (Supplementary Figure 2C).

The Depletion of Eosinophils During Fasciolosis Leads to More Severe Clinical Signs and Liver Damage
We then analyzed the function of eosinophils during F. hepatica infection. To this end, infected mice were treated with a Siglec-F specific antibody during the infection to deplete eosinophils.
As control, an IgG 2a isotype antibody was used. Interestingly, eosinophil in vivo depletion was associated with increased severity of clinical signs induced by F. hepatica infection and liver damage characterized by higher necrosis and fibrosis ( Figure 4A). The depletion of eosinophils was confirmed by flow cytometry (Supplementary Figure 5A), both in PECs and livers of infected animals ( Figure 4B and Supplementary Figure 5B). The decrease of eosinophil levels in PEC was associated with higher levels of CCL11, TSLP, and IL-5 in the peritoneum (Figure 4C), suggesting that infected animals increase the levels of these cytokines and chemokine to promote eosinophil differentiation during infection.

Eosinophils Limit the Production of IL-10 by T Cells and Promote the Production of Th1 and Th2 Type Cytokines
To analyze the effect of eosinophil depletion during experimental fasciolosis, livers and spleens from infected mice treated  . Asterisks correspond to significant differences as follows: *p < 0.05, **p < 0,01, ***p < 0.001, ****p < 0.0001, performed by one way ANOVA followed by Tukey's test.
with anti-Siglec-F or control antibody were removed and the T cell immune response was analyzed by flow cytometry (Supplementary Figure 6). Eosinophil depletion did not affect either the frequency or the cell number of splenic or hepatic CD4 + T cells of infected animals (Figures 5B,C  and Supplementary Figures 6E,F). However, both spleen and hepatic CD4 + T cells from eosinophil-depleted infected animals produced higher levels of IL-10 than CD4 + T cells from control infected mice, while the production of IFNγ and IL-4 was not affected (Figures 5A,B). Splenic or hepatic regulatory CD4 + T cells, defined as FoxP3 + CD4 + T cells did not present any change either in frequency, cell number or IL-10 expression (Figure 5C and Supplementary Figures 6E,F).
control infected mice ( Figure 6A). Moreover, the depletion of eosinophils during experimental fasciolosis was associated to a decreased production of IL-4, IL-5 and IFNγ (Figure 6A), suggesting that in the absence of eosinophils the Th2 or Th1 immune responses are impaired.
Last, the depletion of eosinophil induced higher gene expression of the immunoregulatory cytokine TGFβ and Fizz-1 in the livers of infected animals, although only the increase of TGFβ was significant ( Figure 6B). Altogether, these results indicate that eosinophils limit the regulatory immune response and favor the Th2 or Th1 immune response, which is more effective to limit the damage induced by the parasite infection.

Eosinophils Promote a Humoral Response That Is More Effective in Triggering Degranulation
In order to evaluate whether the depletion of eosinophil affected the humoral response induced during the infection with F. hepatica, we analyzed the antibody titers of parasitespecific antibodies. Eosinophil depletion did not alter the titers of parasite-specific IgM and IgA antibodies ( Figure 7A). However, eosinophil-depletion induced a significant increase in the specific IgG antibody titers (Figure 7A), which was associated with an increase in IgG 1 and a decrease of IgG 3 antibodies with respect to the control infected group, while no changes were detected with IgG 2a and IgG 2b titers ( Figure 7B).
Interestingly, the antibodies induced during the infection were capable of inducing degranulation of eosinophils, while antibodies from non-infected animals did not ( Figure 7C). Furthermore, antibodies from control infected animals induced higher levels of degranulation than antibodies from eosinophildepleted mice. Altogether, these results indicate that eosinophils modulate the effector mechanisms mediated by parasite-specific antibodies during fasciolosis.

DISCUSSION
In this study, we investigate the role of eosinophils during F. hepatica infection and show that they are beneficial to the host by promoting an effective immune response that partially controls liver damage. Accumulation of eosinophils is a well-documented feature of helminth infections since these cells can be vital to develop Th2 immune responses against parasitic helminths (22). However, their role during infection is rather controversial. Experimental infections either with the nematodes Brugia malayi (23), Nippostrongylus brasiliensis (24), FIGURE 7 | The presence of eosinophils promotes the production of antibodies with higher capacity to trigger degranulation. Mice (n = 7) received an intraperitoneal injection of 15 µg of monoclonal rat IgG 2a anti-Siglec-F antibody the day before and after infection with F. hepatica and every 3 days until sacrifice. The control group consisted of mice injected intraperitoneally with isotype control antibody. Mice were bled at 20 d.p.i. (A) Parasite-specific IgG, IgA, and IgG antibodies were detected by ELISA on FhTE coated plates. Results obtained for one representative experiment out of two are shown (n = 9). (B) Parasite-specific IgG 1 , IgG 2a , IgG 2b , and IgG 3 antibodies were detected by ELISA on FhTE coated plates. (C) Degranulation of bone marrow derived-eosinophils (1 × 10 6 /well) from infected animals was evaluated by determining the eosinophil peroxidase enzyme liberation on eosinophils incubated in the presence of serially diluted sera from infected and non-infected mice with FhTE (100 µg/ml) in the presence of OPD and H 2 O 2 . (D) Sera (diluted 1/50) from eosinophil-depleted and control mice were incubated with bone marrow derived-eosinophils (1 × 10 6 /well) in the presence of FhTE (100 µg/ml), OPD and H 2 O 2 for 3 h at 37 • C. Degranulation was evaluated by determining the liberation of eosinophil peroxidase to the culture medium. Results obtained for one representative experiment out of two are shown (n = 9). Asterisks correspond to significant differences as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, performed by one way ANOVA followed by Tukey's test.
First, we identified eosinophils during F. hepatica infection and showed that they augment with the time of infection, a feature that also characterizes infections by B. malayi (23), N. brasiliensis (24), A. cantonensis (25,26). In line with our results, eosinophils constitute 40-60% of the cells in the peritoneal cavity of B. malayi or S. mansoni-infected mice (23,30).The parasite itself could promote the accumulation of greater number of eosinophils in the peritoneal cavity after invading the intestinal wall that allows the juvenile flukes to reach the peritoneal cavity. However, this occurs in early stages of the infection (3). Moreover, eosinophils could accumulate in the peritoneum in response to the presence of immature parasites in the peritoneal cavity, even at 21 days post-infection (40,41). However, we failed at detecting parasites in the peritoneum in our experimental infections in mice. Last, eosinophils could also derive from the blood due to portal hypertension produced by fibrosis in livers (42), since infected mice with severe clinical signs present hemorrhagic ascites which is a marker of advanced liver injury (43,44). Thus, the eosinophils found in the peritoneal cavity could represent an attempt of the host to allow eosinophil recruitment to the liver to fight against the parasite.
Eosinophilia in the peritoneum was associated with high levels of essential factors for the development, recruitment, activation and maintenance of eosinophils, such as TSLP and IL-5 from the first week post-infection and CCL11 from the second week postinfection, reflecting a suitable milieu for eosinophil activation and differentiation (23). Indeed, the depletion or deficiency of IL-5 or CCL11 or blockade of IL-5R abrogates eosinophilia in several helminth infections (18,23,28,38,(45)(46)(47). In addition, both hepatic and peritoneal eosinophils expressed CD44, CD162, and CD11b, molecules involved in cell adhesion during inflammatory conditions (48), indicating an activated state in these cells (49)(50)(51). Furthermore, according to the levels of these molecules, hepatic eosinophils seem to be more activated than peritoneal ones, a feature that could be associated with their effector mechanisms (52,53). Also, the expression of high levels of Sirpα by both hepatic and peritoneal eosinophils from F. hepatica infected mice might promote their survival, since it regulates their homeostasis, by regulating the degranulation process and inducing signals during inflammation (54). Last, the absence of MHC class II and co-stimulatory molecules rules out the possibility for eosinophils to act as non-professional antigenpresenting cells in the context of F. hepatica infection (55,56).
The depletion of eosinophils during infection with F. hepatica partially protected mice from clinical signs and liver damage, indicating that they contribute to the defense mechanisms induced by the host. Indeed, our results strongly suggest that eosinophils would act by two complementary mechanisms: by limiting the production of IL-10 by CD4 + T cells and by promoting degranulation. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses, and by down-regulating the production of Th1 cytokines (5,7,57,58). This immuneregulated environment favors the differentiation of regulatory T cells (7), a process that is mainly mediated by regulatory dendritic cells (4). Thus, a possibility is that eosinophils modulate dendritic cell function and limit the differentiation of Treg. Preliminary results from our laboratory suggest that eosinophils favor dendritic cell migration to the spleen, since a lower frequency of CD11c + cells was detected in spleens from eosinophil-depleted mice (Supplementary Figure 7). Nevertheless, further data are necessary to determine whether dendritic cells from eosinophil-depleted mice have a higher capacity to differentiate Tregs. Furthermore, apart from limiting the production of IL-10 by T cells, eosinophils seem to favor the differentiation of Th2 type T cells, as judged by the strong production of IL-4 and IL-5 by stimulated splenocytes. The higher levels of IL-10 in eosinohil-depleted infected mice could be related to an increase of liver damage. In agreement with our results, EPO or major basic protein-deficient mice infected with Litomosoides sigmodontis present increased levels of IL-10 in the thoracic cavity, and a decrease in IL-5, which were accompanied by increased numbers of worms in infected mice (59,60). Interestingly, IL-10 is associated with susceptibility of helminth infection (60) or with liver lesions caused by liver inflammation (61) or by helminth infection (62,63).
On the other hand, eosinophils can directly injure or kill helminths by releasing a battery of toxic enzymes stored in their granules, although only in vitro evidences are available (64). In most cases, eosinophils require cooperation with antibodies or complement for killing capacity (65)(66)(67). Published results demonstrate that antibodies induced during F. hepatica infection can adhere to eosinophils and induce eosinophil degranulation in the presence of juvenile flukes (68, 69) by a mechanism where eosinophil proteins might be involved (70) or by eosinophil entrapment through extracellular traps (71). Our results highly suggest that eosinophils promote an antibody response that is more effective in mediating their degranulation. Mouse eosinophils are characterized by the expression of FcγRIII low affinity activating receptor that binds to IgG 2a and IgG 2b antibodies (72). The higher capacity of the sera from infected animals to induce degranulation was not associated with antibody titers since both groups had similar IgG 2a and IgG 2b titers. Thus, these antibodies might possess an advantage in terms of recognition of parasite antigens, although further experiments are required to confirm this hypothesis. Last, the higher IgG 1 titers produced by eosinophil-depleted infected mice could be associated with the more severe liver damage that presented these animals, in an effort to contribute to parasite elimination, even though these antibodies do not seem to have a role in eosinophil degranulation. Of note, IgE would not play a role in eosinophil degranulation in F. hepatica infected-mice since the specific Fc receptors are not expressed on murine eosinophils (73).
In conclusion, our work demonstrates the beneficial immunomodulatory roles of eosinophils during F. hepatica infection by limiting IL-10 production and enhancing the capacity of specific antibodies to induce eosinophil degranulation, thus contributing to the understanding of eosinophil function during helminth infections.

DATA AVAILABILITY STATEMENT
All datasets presented in this study are included in the article/Supplementary Material.

AUTHOR CONTRIBUTIONS
SF performed the experiments, analyzed the data, and drafted the manuscript. VC, MC, MF, ML, and SR-Z participated in the sample collection, cell culture, and flow cytometry analyses. SH and JT helped with the microscopy studies. TF conceived the whole work, designed the experiments, interpreted the data, and drafted the manuscript. All authors gave final approval of the version to be published.