TY - JOUR AU - Pievani, Alice AU - Savoldelli, Roberto AU - Poelchen, Juliane AU - Mattioli, Elisa AU - Anselmi, Giorgio AU - Girardot, Alice AU - Utikal, Jochen AU - Bourdely, Pierre AU - Serafini, Marta AU - Guermonprez, Pierre PY - 2021 M3 - Review TI - Harnessing Mesenchymal Stromal Cells for the Engineering of Human Hematopoietic Niches JO - Frontiers in Immunology UR - https://www.frontiersin.org/articles/10.3389/fimmu.2021.631279 VL - 12 SN - 1664-3224 N2 - Tissue engineering opens multiple opportunities in regenerative medicine, drug testing, and modeling of the hematopoiesis in health and disease. Recapitulating the organization of physiological microenvironments supporting leukocyte development is essential to model faithfully the development of immune cells. Hematopoietic organs are shaped by spatially organized niches defined by multiple cellular contributions. A shared feature of immune niches is the presence of mesenchymal stromal cells endowed with unique roles in organizing niche development, maintenance, and function. Here, we review challenges and opportunities in harnessing stromal cells for the engineering of artificial immune niches and hematopoietic organoids recapitulating leukocyte ontogeny both in vitro and in vivo. ER -