Epigallocatechin Gallate Can Protect Mice From Acute Stress Induced by LPS While Stabilizing Gut Microbes and Serum Metabolites Levels

Epigallocatechin gallate (EGCG) has potent biological activity as well as strong antioxidant and anti-inflammatory effects. This study aims to explore the protective effect of EGCG on LPS-induced acute injury. We randomly divided 18 mice into three groups: CON, LPS, and EGCG-LPS. We gave the EGCG-LPS group gavage treatment with EGCG on day 8–15 and an intraperitoneal injection of LPS on day 16 to induce acute injury. The results showed that, compared with the LPS group, the bodyweight of the mice in the EGCG-LPS group increased significantly and effectively inhibited the morphological damage of the jejunum and liver. We measured liver tissue and found that the EGCG gavage treatment significantly inhibited the pro-inflammatory factors (TNF-α, IL-1β, IL-6, MCP-1, MIP-2, IFN-γ) and oxidation indicators (MPO, NO, ALT, and AST) levels increase. The microbiological results showed that the EGCG gavage treatment reshaped the disturbance done to the intestinal microbial community in the mice by LPS, reversed the changes in the abundance ratio of Firmicutes/Bacteroidetes, and significantly reduced the abundance of Enterobacteriales. Finally, the serum metabolomics results showed that, when compared with the LPS group, the gavage treatment of EGCG significantly increased the concentration of sphingomyelin (d17:1/17:0), sphingomyelin (d16:1/20:0), and significantly reduced the content of trans-Hexadec-2-enoyl carnitine, and so on. Therefore, we believe that EGCG can protect mice from acute stress induced by LPS while stabilizing gut microbes in general, improving the metabolism of sphingolipids, and inhibiting the content of harmful metabolites.


INTRODUCTION
Lipopolysaccharide (LPS) is a major part of the cell wall of gram-negative bacteria, and it is composed of lipids and polysaccharides. A large amount of experimental data shows that LPS can cause systemic inflammatory response syndrome (SIRS) (1,2). It is an endotoxin that can not only be secreted in the form of outer membrane vesicles (OMVs) but can also be released by forming micelles in the bacterial membrane (3). LPS is transported to the cytoplasm of immune cells in the form of OMVs, and inflammatory caspases, as the specific receptors of LPS, can activate the production of downstream pro-inflammatory factors (4). On the other hand, LPS contained in micelles is recognized by TLR4 under the combined action of binding proteins (5). Finally, the danger signal of the stress inflammation induced by LPS will reach the liver from the gastrointestinal tract, portal vein, or arterial blood circulation (6). LPS activates liver immune cells (Kupffer cells), thereby inducing the production of TNF-a and other inflammatory factors, which cause the liver to produce oxidative stress and inflammation (7). In an injured state, the liver will eliminate the damaged organelles through autophagy to reduce apoptosis, maintain the integrity of the tissue genome, and provide energy to ensure cell homeostasis (8). In addition, LPS and its receptor protein, TLR4, can induce small intestine damage and reduce its nutrient absorption capacity, thereby causing an imbalance of the intestinal flora and increasing the intestinal permeability. This process further promotes liver damage (9). This may be why the microbial metabolites after the disorder can promote liver inflammation and fibrosis (10). Therefore, for intestinal microbial homeostasis, we can use intestinal microbes to regulate the liver physiology through the intestinal liver axis (11).
Epigallocatechin gallate (EGCG) is one of the most abundant catechins in green tea and is a typical polyphenol flavonoid compound that has eight free hydroxyl groups (12). Extensive studies have shown that EGCG is the most widely active substance in green tea, with antibacterial, antiviral, antioxidant, anti-arteriosclerosis, anti-thrombosis, anti-vascular proliferation, anti-inflammatory, and anti-tumor effects (12,13). Cancer is the result of the final pathogenic development of inflammation (14). Many inflammatory factors, including proinflammatory factors and chemokines, mediate the occurrence of cancer (15,16). In inhibiting inflammation and cancer, EGCG has shown good performance significantly inhibiting inflammatory cell expression in various cancer cells (17)(18)(19). Clinical studies have shown that EGCG can resist the proliferation and migration of human cancer cells by inhibiting the expression of NF-kB and MMP-9 (20) On the other hand, EGCG can maintain the integrity of the epithelial barrier of Caco-2 cells and inhibit the increase in the intestinal epithelial permeability induced by IFN-g (17). After the gut microbes (including Enterobacter aerogenes, Raoultella planticola, Klebsiella pneumoniae) hydrolysis EGCG, the host gut can absorb these microbial metabolites (21). According to previous studies, after EGCG is hydrolyzed in the intestine, a series of transformations and degradations will occur. Studies have shown that 5-(3,5-dihydroxyphenyl)-4-hydroxyvaleric acid is the main metabolite of EGCG in the intestine, and, after the intestinal microbes degrade EGCG, 5-(3',5'-dihydroxyphenyl) -gvalerolactone is absorbed by the body, and its glucuronic acid form is the main urinary metabolite (22). EGCG is first absorbed by the intestine after being ingested, and, in this process, the intestinal flora plays a vital role (23). On the other hand, EGCG also has a significant regulatory effect on the composition of the intestinal microbial community. The addition of EGCG to the diet can reduce the microbial abundances of Alipipes, Anaerotruncu, and Desulfovibrio, which are related to metabolic disorders, and can remedy the malnutrition of the intestinal flora caused by non-alcoholic steatohepatitis. EGCG can also inhibit Prevo and Fusobacterium to regulate the intestinal flora (17). Therefore, we assume that EGCG can maintain the gut microbial community's structural homeostasis and serum metabolites to reduce an acute host injury. This study explored the protection of EGCG in mice with an acute injury induced by lipopolysaccharide, observed the changes in the intestinal flora and serum metabolites of these mice, and determined the protective effect of EGCG.

Animals and Experimental Design
Animal experiments were performed according to the Guidelines for the Care and Use of Laboratory Animals of Hunan Agricultural University. We selected 18 eight-week-old ICR mice (Hunan Sileike Jingda Co, Changsha, China) for a week of adaptation. On the eighth day, we randomly divided them into three groups: the control group (CON), the LPS model (LPS), and the EGCG gavage treatment group (EGCG-LPS). On days 8-15 of the experiment, according to the pre-experiment results (Supplemental Figures 1, 2), we gave the EGCG-LPS group a gavage of EGCG (200 mg/kg), and the other two groups received normal saline gavage of the same dose. On the 15th day of the experiment, we administered LPS (15 mg/kg) intraperitoneal injections in the mice in the LPS and EGCG-LPS groups as well as intraperitoneal injections of normal saline of the same dose in the CON group ( Figure 1A). On the 16th day, all mice were sacrificed, serum was collected for metabolome analysis, a portion of the liver and jejunum were fixed in 4% formalin for histomorphological analysis, and a portion of the liver and colon contents were taken, quick-frozen in liquid nitrogen, and used for liver index determination and 16srDNA determination.

Jejunum and Liver Histopathology
The tissue fixed in 4% formalin was treated with gradient concentrations of xylene and ethanol and then embedded in paraffin. The embedded sample was dehydrated and transparent; it was dyed with hematoxylin and eosin and then covered with neutral gum. The specific process was based on our previous research (24).

Determination of Proinflammatory Factors and Oxidative Stress Substances in Liver Tissue
The concentrations of TNF-a, IL-1b, IL-6, MCP-1, MIP-2, IFN-g and MPO in the liver tissue samples were measured by a Mouse ELISA Kit (Jiangsu Yutong Biological Technology Co) with a microplate reader (Bio Tek, USA). The concentration of NO as well as the enzyme activity of ALT and AST in the liver tissue samples were respectively measured by a Nitric Oxide (NO) assay kit (Microwell plate method), Alanine aminotransferase Assay Kit and Aspartate aminotransferase Assay Kit (Nanjing Jiancheng Bioengineering Institute) with a microplate reader (Bio Tek, USA).

Serum Metabolomic Analyses
W e e x t r a c t e d th e s e r u m wi t h m e t h a n o l a n d a d d dichlorophenylalanine. After mixing the liquid, we centrifuged it to obtain the supernatant and perform an on-machine test of LC-MS. The specific chromatographic column and liquid phase conditions used referred to our previous research. The offmachine data was preprocessed with Compound Discoverer software to obtain the molecular weight, mass-to-charge ratio, retention time, and peak area. Finally, we used SIMCA14.1 to perform orthogonal partial least squares discrimination analysis on the data to obtain the VIP value. We used the Human Metabolome Database (https://hmdb.ca/spectra/ms/search) for substance comparison.

16S Ribosomal RNA Amplicon Sequencing
We used the QIAamp DNA Stool Mini Kit to extract microbial genomic DNA from the colon contents. We selected the V3-V4 region sequence of 16S rDNA for high-throughput sequencing analysis on the Illumina platform. The specific process was based on our previous research (24). We then used Trimmomatic software to perform quality control and optimization of the offmachine data before finally performing operational taxonomic unit (OTU) clustering to determine the composition of the microbial communities at different taxonomic levels. Additionally, we used mothur (Version 1.33.3) to perform alpha diversity analysis. The composition of the microbial community was generated by BMKCloud (http://www.biocloud.net/). The abundance of the sequenced microorganisms at different taxonomic levels was uploaded to http://huttenhower.sph. harvard.edu/galaxy for linear discriminant analysis (LDA) and LDA effect size (LEfSe) analysis.

Data Analysis
All of the data in this study are expressed as mean ± standard deviation (SD). The data were analyzed using SPSS 22.0. The differences between the means of the experimental groups were analyzed using one-way analysis of variance and Tukey's multiple comparison test. A p value < 0.05 was regarded as a significant difference.

EGCG Improves the Acute Injury Induced by LPS in Mice
Before the last intraperitoneal injection of LPS, we administered EGCG gavage for 7 consecutive days. The results showed that: compared with the CON group, the intraperitoneal injection of LPS significantly reduced the weight of the mice. But the mice that received EGCG gavage treatment, compared with the LPS A B FIGURE 1 | The protective effects of intragastric administration of EGCG on body weight. (A) the experimental process; (B) body weight. Data are mean ± SD (n = 6) and analyzed by one-way ANOVA. "*" means that the horizontal line connects the p-value of the data between the two groups <0.05, and the "P" value in the figure represents the accurate value of p between the three groups of data, and some of the p values between the three groups are too small to be expressed as p<0.0001. group, the weight was significantly increased and avoid the weight loss of mice ( Figure 1B, p < 0.05).

EGCG Inhibits the Development of Acute Injury Induced by LPS
The results of jejunum histomorphology analysis (Figures 2A-F) showed that, compared with the CON group, the intraperitoneal injection of LPS caused a significant decrease in the height of the mouse jejunum villi and a significant increase in the depth of the crypts (p < 0.05). However, EGCG-LPS group, compared with the LPS group, the mice had significantly increased villus heights, while the crypt depth was reduced considerably (p < 0.05). It returned to a normal level similar to that of the CON group. The morphological results of the liver tissue ( Figures 2G-I) showed that the liver cells in the CON group were arranged regularly and neatly centered around the central vein. The liver cells in the LPS group showed apparent inflammatory damage, and the cells were arranged irregularly and exhibited inflammatory infiltration. These damages phenomena were greatly alleviated in the EGCG-LPS group.

EGCG Inhibits Inflammation and Oxidative Stress in Liver Tissue
We measured the concentration of inflammatory factors in the liver tissues ( Figures 3A-F), and the results showed that, when compared with the CON group, the intraperitoneal injection of LPS significantly increased the concentration of TNF-a, IL-1b, IL-6, MIP-2, and IFN-g in the liver tissue (p < 0.05). However, in the EGCG-LPS group received EGCG gavage protection, compared with the LPS group, the concentrations of TNF-a, IL-1b, IL-6, MCP-1, MIP-2, IFN-g in the liver tissues of the mice were significantly reduced (p < 0.05). The oxidative stress index of the mouse liver tissue (Figures 3G-J) showed that, when compared with the CON group, the concentration of NO as well as the enzyme activity of ALT and AST in the liver tissue of the LPS group increased significantly (p < 0.05). Compared with the LPS group, the concentration of NO and MPO as well as the enzyme activity of ALT and AST in the mice's liver tissue after receiving the EGCG treatment decreased significantly (p < 0.05). However, the alleviation of this oxidative stress index did not return to the same level as the CON group. Data are mean ± SD (n = 6) and analyzed by one-way ANOVA. "*" means that the horizontal line connects the p-value of the data between the two groups <0.05, and the "P" value in the figure represents the accurate value of p between the three groups of data, and some of the p values between the three groups are too small to be expressed as p<0.0001.

EGCG Regulates Intestinal Microbes in Mice With Acute Injury Induced by LPS
We first analyzed the 16S rRNA V3-V4 region sequence of colonic microorganisms to obtain the a diversity of intestinal microorganisms ( Figures 4A-F). Compared with the CON group, the observed species, Shannon index, Simpson index, Chao index, and ACE index in the LPS group all decreased significantly (p < 0.05). However, in the mice that received the EGCG treatment, compared with the LPS group, the observed species, Shannon index, Simpson index, Chao index, and ACE index were significantly improved (p < 0.05). Therefore, EGCG gavage can significantly protect mice from the decrease in intestinal microbial diversity caused by LPS.
The microbial community composition of the mouse colon ( Figures 5A-D) showed that Firmicutes, Proteobacteria, and Bacteroidetes were the three main microorganisms at the phylum level. The abundance of these three microorganisms accounts for more than 85% of all microorganisms. The Firmicutes abundance in the CON, LPS, and EGCG-LPS groups were 78.24656%, 18.58981%, and 39.05929%, respectively. Proteobacteria abundance accounted for 4.079801%, 37.06216%, and 12.85193%, respectively. The Bacteroidetes abundance was 9.898671%, 32.38386%, and 31.01083%, respectively. Compared with the CON group, the abundance of Firmicutes in the LPS group was significantly reduced (p < 0.05), but the abundance of Proteobacteria and Bacteroidetes was significantly increased (p < 0.05). Compared with the LPS group, the abundance of Firmicutes in the EGCG-LPS group was significantly increased (p < 0.05), but the abundance of Proteobacteria was significantly reduced (p < 0.05).
The microbial community composition of the mouse colon ( Figures 5E-H) showed that Lactobacillales, Bacteroidales, and Clostridiales were the three main microorganisms at the order level. The abundance of these three microorganisms accounts for more than 80% of all microorganisms. The Lactobacillales abundance in the CON, LPS, and EGCG-LPS groups were 66.42404%, 10.58623%, and 12.08513%, respectively. The Bacteroidales abundance was 9.873076%, 32.36142%, and 30.93685%, respectively. The Clostridiales abundance was 5.006486%, 5.806248%, and 24.00442%, respectively. Compared with the CON group, the abundance of Lactobacillales in the LPS group was significantly reduced (p < 0.05), but the abundance of Enterobacteriales was significantly increased (p < 0.05). Compared with the LPS group, the abundance of Clostridiales in the EGCG-LPS group was significantly increased (p < 0.05), but the abundance of Enterobacteriales was significantly reduced (p < 0.05). Data are mean ± SD (n = 6) and analyzed by one-way ANOVA."*" means that the horizontal line connects the p-value of the data between the two groups <0.05, and the "P" value in the figure represents the accurate value of p between the three groups of data, and some of the p values between the three groups are too small to be expressed as p<0.0001.
The microbial community composition of the mouse colon ( Figures 5I-L) showed that Enterobacteriaceae, Lactobacillus, Akkermansia, Helicobacter, and Bacteroides were the five main microorganisms at the genus level. The abundance of these five microorganisms accounts for more than 70% of all microorganisms. The Enterobacteriaceae abundance in the CON, LPS, and EGCG-LPS groups was 1.453315%, 33.92974%, and 9.697065%, respectively. The Lactobacillus abundance was 66.11865%, 10.09782%, and 11.92314%, respectively. The Akkermansia abundance was 0.545563%, 3.846639%, and 3.939904%, respectively. The Helicobacter abundance was 1.53536%, 6.888258%, and 10.97262%, respectively. The Bacteroides abundance was 1.146524%, 18.53617%, and 9.785772%, respectively. Compared with the CON group, the abundance of Lactobacillus in the LPS group was significantly reduced (p < 0.05), but the abundances of Enterobacteriaceae and Bacteroides were significantly increased (p < 0.05). Compared with the LPS group, the abundance of Enterobacteriaceae in the EGCG-LPS group was significantly reduced (p < 0.05).
We used linear discriminant analysis (LDA) to estimate the effect of the abundance of each component (species) on the difference effects ( Figure 6A). LEfSe analysis ( Figure 6B) showed that the CON group was significantly enriched in Lactobacillus, Lactobacillaceae and Lactobacillales. The LPS group was significantly enriched in Enterobacteriaceae and Enterobacteriales. Meanwhile, the EGCG-LPS group was significantly enriched in Clostridiales.

EGCG Affects the Serum Metabolomic Profiles During Acute Injury
We used GC-MS to determine the serum metabolites from the acute injury induced by LPS in the mice. First, we used orthogonal partial least squares discrimination analysis (OPLS-DA) to establish a model of the relationship between metabolite expression and sample category to predict the sample category. At the same time, we also verified this model. The results showed that the serum metabolites of the different groups changed significantly, and the similarity of these metabolisms within each group was high (Figure 7). We specifically analyzed the different metabolites between the CON and LPS groups. We found that the serum levels of the Trihexosylceramide (d18:1/ 16:0), trans -Hexadec -2 -enoyl carnitine, 4 -Hydroxytamoxifen, 2 -arachidonoylglycerol, Protoporphyrinogen IX, Janthitrem F, Glycochenodeoxycholic acid 3 -glucuronide, 3hydroxytridecanoyl carnitine, Pentadecanoylglycine and Palmitoylglycine significantly increased after the acute injury induced via LPS in the mice ( Figure 8A

DISCUSSION
This study explored the protective effect of EGCG on LPSinduced acute injury. We observed that EGCG can significantly prevent the morphological damage done to the jejunum and liver caused by LPS. At the same time, EGCG gavage treatment can also significantly inhibit LPS-induced liver inflammation and oxidative stress. The 16srDNA results show that EGCG can maintain the structure of the intestinal microbial community and inhibit the abundance of enteric pathogens, such as Enterobacteriaceae. Via serum metabolomics analysis, we posit that glycerophospholipid and sphingolipid substances are promoted by EGCG, thereby improving the organism's immune function.
Polyphenols have been widely used to improve the We found that the gavage of EGCG significantly increased the content of pyridoxal in the serum of the mice, thereby enhancing their ability to reduce the expression of IL-1b, TNF-a, IL-6, and other pro-inflammatory factors while also enhancing the organisms' immunity due to their good biological activity (25)(26)(27). Studies have shown that polyphenols, including EGCG and theaflavin-3′-O-gallate (TF3′G), are absorbed in the jejunum after being ingested (28). Therefore, the nutrient absorption capacity of the jejunum is particularly important for the bioavailability of Compared with the CON group, ten characteristic metabolites increased in the LPS group; (B) Compared with the CON group, ten characteristic metabolites reduced in the LPS group; (C) Compared with the LPS group, ten characteristic metabolites increased in the EGCG-LPS group; (D) Compared with the LPS group, ten characteristic metabolites reduced in the EGCG-LPS group.
EGCG. T The jejunal epithelium can be renewed in three days, and the cells on the intestinal villi are replaced via the proliferation of crypt cells (29). The role of the intestinal villi is to increase the area of absorption while also promoting absorption (30). Extensive studies have shown that the acute damage done by LPS to the organism can lead to the destruction of the intestinal integrity and barrier function (31,32). Our research shows that gavage treatment with EGCG can protect the jejunum's structural integrity and its ability to absorb nutrients so that it will not be affected by an acute injury caused by LPS. When LPS enters the organism, TLR4 in the liver cells can actively recognize LPS and activate the expression of downstream pro-inflammatory factors (33). Pro-inflammatory factors, including the IFN-g, TNF-a, IL-17, and IL-1 family are widely expressed in the liver, which further deepens the inflammatory damage done to the liver (34). Our research shows that EGCG can well inhibit LPS from activating TNF-a, IL-1b, IL-6, MCP-1, MIP-2, and IFN-g. Although the concentration of MCP-1 and MPO was not statistically significant between the CON and LPS groups, from a data point of view, these two indicators still showed an upward trend between these two groups, which proves that LPS induced the occurrence of acute stress. Moreover, under the protection of EGCG, these two indicators were significantly reduced when compared with the LPS group, and, at the same time, there was no significant difference between the EGCG group and CON group. After being injured, the liver will feedback this stress to the intestine through the portal vein, and this process reshapes the structure of the intestinal microbial community (35). Henao-Mejia's research team found that the immersion of a large number of Toll-like receptors in the portal vein can significantly promote the expression of TNF-a and further induce the development of liver inflammation damage (36). After this signal pathway is transmitted from the portal vein into the intestine, it leads to the intestinal microbes' structural changes. Bacterial genera, bacteroides, and Ruminococcus are significantly increased, while Prevotella is significantly reduced (37). Our research also found that the intraperitoneal injection of LPS significantly increased the abundance of bacterial genera and Bacteroides. Although the gavage of EGCG failed to inhibit the increase in the abundance of Bacteroides, it did regulate Firmicutes/Bacteroidetes. This ratio is also a characteristic of the stability of the intestinal microbial community (38). However, EGCG treatment given by oral gavage did not prevent the decrease in the abundance of Lactobacillales, similarly to the previous report showing that diet supplementation with EGCG has a small effect on the abundance of Bifidobacteria (39). Gut microbes play an irreplaceable role in intestinal immunity. Proteobacteria is the intestinal microorganism known to be the most relevant to diseases (40). Most of the microorganisms belonging to this phylum level are human pathogens. An increasing number of studies have found that Proteobacteria is an important cause of intestinal inflammation and metabolic disease (41,42). In this experiment, the gavage treatment of EGCG significantly reduced the abundance of Proteobacteria underwater at the portal level, thereby reducing the risk of intestinal inflammation in the mice. Enterobacteriales is an order under Gamma-proteobacteria, which is under Proteobacteria. The gavage treatment of EGCG also suppressed the abundance of such pathogenic bacteria. EGCG can inhibit the expression of stx1 and RecA in enterobacteria membrane cells and induce the expression of stx2 and oxyR by increasing the oxidative stress response, thereby increasing the permeability of the outer membrane and leading to the inactivation of Enterobacter (43).
As an important part of systems biology, metabonomics is used in the prediction of the effects of diseases and tumor drugs as well as the detection of markers (44). Sphingolipids are an essential part of the structure of biological membranes, and an increasing number of studies have shown that they are closely related to immunity and inhibiting inflammation (45,46). Sphingomyelin metabolites can reduce the ability of TNF to induce CCL5 and minimize the risk of inflammation. Our research showed that, under the protection of EGCG gavage, the serum levels of sphingomyelin (d17:1/17:0) and sphingomyelin (d16:1/20:0) were not affected by the LPSinduced acute injury. Pyridoxal is an active form of vitamin B6, which has always been regarded as an important substance for improving immune function and reducing inflammation (47). We found that the gavage of EGCG significantly increased the content of pyridoxal in the serum of the mice, thereby enhancing their ability to reduce the expression of IL-1b, TNF-a, IL-6, and other pro-inflammatory factors while also enhancing the organisms' immunity. More importantly, previous studies have shown that vitamins have an inhibitory effect on the Stx2 production of Enterobacteriales, which reduces the growth and virulence potential of this type of intestinal pathogenic bacteria (48). This corresponds to the fact that under the protection of EGCG gavage in our experiment, the abundance of Enterobacteriales in the colon is significantly reduced. On the other hand, the reduction of harmful substances also reflects the effect of EGCG gavage therapy, which significantly reduced the content of trans-Hexadec-2enoyl carnitine in the serum of the mice with LPS-induced inflammation. Trans-Hexadec-2-enoyl carnitine is a kind of acylcarnitine. The accumulation of acylcarnitine in the body promotes the expression of pro-inflammatory factors in the organism and induces the phosphorylation of JNK and ERK (49). Studies have also found that an increased concentration of acylcarnitine increases the risk of intestinal permeability. It can be seen that the gavage treatment of EGCG significantly increased the content of immune-enhancing substances in the blood and inhibited the connection between harmful substances and inflammation.

CONCLUSIONS
Our experimental results show that the gavage of EGCG can effectively prevent the negative effects of LPS-induced acute injury. On the other hand, EGCG can stabilize the intestinal microbial community structure of mice in general and reduce pathogenic bacteria, such as Proteus and Enterobacter. Moreover, EGCG can regulate serum metabolite components; promote the production of beneficial metabolomes, including sphingomyelin (d17:1/17:0), sphingomyelin (d16:1/20:0); and reduce the production of these harmful metabolomes, including trans-Hexadec-2-enoyl carnitine. Therefore, EGCG can significantly inhibit the acute injury induced by LPS and improve the immune function of the organism.

DATA AVAILABILITY STATEMENT
The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://www.ncbi. nlm.nih.gov/, SUB8703003.

ETHICS STATEMENT
The animal study was reviewed and approved by Animal Care and Use Committee of Hunan Agricultural University.

AUTHOR CONTRIBUTIONS
YM performed the study and conducted data analysis. GL designed the research. MT provided assistance for the study.
HJ and JF prepared the first draft of the manuscript. All authors contributed to the article and approved the submitted version.