%A Li,Siting %A Song,Guang %A Bai,Yina %A Song,Ning %A Zhao,Jiuliang %A Liu,Jian %A Hu,Chaojun %D 2021 %J Frontiers in Immunology %C %F %G English %K protein microarray,Proteome,Biomarker Discovery,autoimmune disease,experimental design %Q %R 10.3389/fimmu.2021.645632 %W %L %M %P %7 %8 2021-May-03 %9 Review %+ Jian Liu,Department of Rheumatology, Aerospace Center Hospital, Aerospace, Clinical Medical College, Peking University,China,2120044180@qq.com %+ Chaojun Hu,Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Key Laboratory of Rheumatology & Clinical Immunology, Ministry of Education,China,2120044180@qq.com %+ Chaojun Hu,Department of Rheumatology, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID),China,2120044180@qq.com %# %! N/A %* %< %T Applications of Protein Microarrays in Biomarker Discovery for Autoimmune Diseases %U https://www.frontiersin.org/articles/10.3389/fimmu.2021.645632 %V 12 %0 JOURNAL ARTICLE %@ 1664-3224 %X Dysregulated autoantibodies and cytokines were deemed to provide important cues for potential illnesses, such as various carcinomas and autoimmune diseases. Increasing biotechnological approaches have been applied to screen and identify the specific alterations of these biomolecules as distinctive biomarkers in diseases, especially autoimmune diseases. As a versatile and robust platform, protein microarray technology allows researchers to easily profile dysregulated autoantibodies and cytokines associated with autoimmune diseases using various biological specimens, mainly serum samples. Here, we summarize the applications of protein microarrays in biomarker discovery for autoimmune diseases. In addition, the key issues in the process of using this approach are presented for improving future studies.