AUTHOR=Wang Yuanyuan , Gu Tingxuan , Tian Xueli , Li Wenwen , Zhao Ran , Yang Wenqian , Gao Quanli , Li Tiepeng , Shim Jung-Hyun , Zhang Chengjuan , Liu Kangdong , Lee Mee-Hyun 

TITLE=A Small Molecule Antagonist of PD-1/PD-L1 Interactions Acts as an Immune Checkpoint Inhibitor for NSCLC and Melanoma Immunotherapy

JOURNAL=Frontiers in Immunology

VOLUME=Volume 12 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.654463

DOI=10.3389/fimmu.2021.654463

ISSN=1664-3224

ABSTRACT=Until now, monoclonal antibody (mAb) targeting the immune checkpoint programmed death 1 (PD-1) and its ligand programmed death ligand-1 (PD-L1) attained enormous success in anticancer therapy; however, due to expensive, poor tumor penetration and high autoimmune side effect level, mAbs cause to limit applying to patients. Here we offer evidence that PD1/PD-L1 inhibitor 1 (PDI-1) as a small-molecule antagonist is capable of diminishing the PD-1/PD-L1 immune checkpoint-mediated exhaustion of T-lymphocytes. PDI-1, predicted by the computational docking model, can bound to human and mouse PD-1 and PD-L1 in vitro and in vivo. PDI-1 could block the interaction between PD-1 and PD-L1 and enhance T cell activity by restoring the TCR-CD28 signaling pathway, thus induce immunogenic cell death of lung cancer and melanoma in vivo. We also investigated the effect of PDI-1 in PD-L1 humanized syngeneic murine model, and the results showed PDI-1 retarded tumor growth of PD-L1 humanized NSCLC and melanoma syngeneic murine model by increasing the T cell infiltrations and its related cytokines.