TY - JOUR AU - Sarabia, Indra AU - Novis, Camille L. AU - Macedo, Amanda B. AU - Takata, Hiroshi AU - Nell, Racheal AU - Kakazu, Juyeon C. AU - Furler, Robert L. AU - Shakya, Binita AU - Schubert, Heidi L. AU - Hill, Christopher P. AU - DePaula-Silva, Ana Beatriz AU - Spivak, Adam M. AU - Trautmann, Lydie AU - Planelles, Vicente AU - Bosque, Alberto PY - 2021 M3 - Original Research TI - Activation of the Anti-Oxidative Stress Response Reactivates Latent HIV-1 Through the Mitochondrial Antiviral Signaling Protein Isoform MiniMAVS JO - Frontiers in Immunology UR - https://www.frontiersin.org/articles/10.3389/fimmu.2021.682182 VL - 12 SN - 1664-3224 N2 - The mitochondrial antiviral signaling protein (MAVS) is part of the cell’s innate immune mechanism of defense. MAVS mRNA is bicistronic and can give rise to a full length-MAVS and a shorter isoform termed miniMAVS. In response to viral infections, viral RNA can be sensed by the cytosolic RNA sensors retinoic acid-inducible gene I (RIG-I) and/or melanoma differentiation-associated protein 5 (MDA5) and activate NF-κB through interaction with MAVS. MAVS can also sense cellular stress and activate an anti-oxidative stress (AOS) response through the activation of NF-κB. Because NF-κB is a main cellular transcription factor for HIV-1, we wanted to address what role MAVS plays in HIV-1 reactivation from latency in CD4 T cells. Our results indicate that RIG-I agonists required full length-MAVS whereas the AOS response induced by Dynasore through its catechol group can reactivate latent HIV-1 in a MAVS dependent manner through miniMAVS isoform. Furthermore, we uncover that PKC agonists, a class of latency-reversing agents, induce an AOS response in CD4 T cells and require miniMAVS to fully reactivate latent HIV-1. Our results indicate that the AOS response, through miniMAVS, can induce HIV-1 transcription in response to cellular stress and targeting this pathway adds to the repertoire of approaches to reactivate latent HIV-1 in ‘shock-and-kill’ strategies. ER -