Phosphorylation of Shrimp Tcf by a Viral Protein Kinase WSV083 Suppresses Its Antiviral Effect

Nuclear DNA-binding TCF proteins, which act as the main downstream effectors of Wnt signaling, are essential for the regulation of cell fate and innate immunity. However, their role during viral infection in shrimp remains unknown. Herein, we demonstrated that Litopenaeus vannamei TCF (LvTcf) acts independently of Lvβ-catenin to promote interferon-like protein LvVago1 production, thus mounting the response to WSSV infection. Further, we observed that WSV083, a WSSV serine/threonine protein kinase, bound to LvTcf and phosphorylated it. Phosphorylated LvTcf was then recognized and degraded via the ubiquitin-proteasome pathway. Moreover, mass spectrometry analyses indicated that the T39 and T104 residues of LvTcf were target sites phosphorylated by WSV083. Point mutation analyses suggested that additional sites of LvTcf may undergo phosphorylation via WSV083. Taken together, the current work provides valuable insights into host immunity and viral pathogenesis. LvTcf is not only a modulator of shrimp innate immunity but is also an important target for WSSV immune evasion. Thus, the current findings will help improve disease control in shrimps.


INTRODUCTION
White spot syndrome virus (WSSV) is the only species within the Whispovirus genus of the Nimaviridae family (1)(2)(3). It is a large double-stranded circular DNA virus with a genome of approximately 300 kb containing 181 open reading frames (ORFs). This virus is a major crustacean pathogen, causing a cumulative mortality of up to 100% in cultured shrimp (4,5). Due to the current lack of effective treatment, understanding the mechanisms of host immunity and host-virus interactions is of great importance for improving WSSV control.
WSSV triggers pattern recognition upon cell entry as the initial step of the innate immune response (6). Shrimp mount humoral and cellular immune responses (7) to defend against viral infection. These rely on several key cell signaling cascades, including the Toll/IMD-NF-kB and JAK/ STAT pathways, among others, which transduce extracellular signals into cells and promote the expression of antimicrobial peptides or other immune effector molecules to combat WSSV infection (8)(9)(10). In its shrimp host, WSSV employs a number of mechanisms to ensure propagation. To this end, the virus hijacks host proteins to facilitate gene transcription. Shrimp NF-kB and STAT were reported to bind the promoter of the WSSV immediate early gene ie1, promoting its expression (11)(12)(13). Viral transcription factor IE1 then drives host JNK autophosphorylation to activate c-Jun and stimulate ie1, wsv056, wsv249, as well as wsv403 expression (14). Through the cell cycle, IE1 and WSV056 competitively interact with Rb to promote the transition from G0/G1 to S phase, providing a favorable environment for viral replication (15). In addition, WSSV employs several strategies to evade host immunity. For example, viral microRNA WSSV-miR-22 restricts host STAT expression by targeting its 3′UTR, which allows for subverting the JAK/STAT-driven antiviral response (16). WSSV can also manipulate metabolic programming to induce the Warburg effect, counteracting reactive oxygen species (17)(18)(19). Moreover, WSSV regulates the degradation of host proteins via ubiquitination-related enzymes encoded by wsv222, wsv249, and wsv403, facilitating viral replication and proliferation (20)(21)(22)(23)(24).
Within research on the relationship between host signaling and viral infection, the Wnt pathway has attracted increasing attention. It is evolutionarily conserved in metazoan animals, regulating cell fate during embryonic development as well as in adult tissues (25)(26)(27). In addition, Wnt/b-catenin/TCF signaling participates in immune regulation. In particular, TCF-1 initiates TFH differentiation, thus promoting the B cell-mediated response to acute viral infections (28). With regard to innate immunity, b-catenin/TCF promotes the expression of type I IFN and interferon-stimulated genes to suppress viral infection (29)(30)(31)(32). In Drosophila, the Dally-mediated Wnt signaling pathway is involved in S2 phagocytosis of WSSV (33). Our previous work showed that the expression of LvWnt5b was initially suppressed in shrimp as a proapoptotic response against WSSV infection. Moreover, Lvb-catenin tends to translocate to the nucleus following WSSV infection where it activates the expression of several antimicrobial peptides (34,35). However, how WSSV regulates the Wnt signaling pathway in shrimp remains unclear. Therefore, further research is necessary in order to determine the relationship between shrimp Wnt signaling and WSSV infection.
Nuclear DNA-binding TCF/LEF proteins from the highmobility group (HMG) box family are the main downstream effectors of the Wnt pathway. In humans, the TCF/LEF family consists of four members designated TCF7, LEF1, TCF7L1, and TCF7L2 (also known as TCF1, LEF1, TCF3, and TCF4, respectively) (36). The majority of TCF isoforms possess a conserved b-catenin-binding domain at the N-terminus and a monomeric HMG domain that recognizes the Wnt response element (5'-ACATCAAAG-3') to mediate DNA binding (37)(38)(39). Importantly, isoforms exhibit different functional properties. In this study, we found that shrimp TCF, referred to as LvTcf, plays a protective role against WSSV infection by promoting the production of shrimp interferon-like protein LvVago1 in an Lvbcatenin-independent manner. Furthermore, we uncovered a new mechanism through which WSSV regulates the Wnt signaling pathway. WSV083, a serine/threonine protein kinase encoded by WSSV (40), was found to bind and phosphorylate LvTcf. Phosphorylated LvTcf was then degraded via the ubiquitinproteasome pathway. Thus, WSV083 suppressed the antiviral effect mediated by LvTcf. The current findings highlight novel therapeutic targets for WSSV control.

Shrimp and Virus
L.vannamei, about 15-20 g, were purchased from a market in Xiamen, China, and kept in air-pumped circulating seawater for 3 days before experiments. WSSV particles (a Chinese isolated) were extracted from hemocytes of infected crayfish Procambarus clarkii and quantified according to Yang's description (41,42).

RNAi Assay
We used RNAi to knock down the expression of LvTcf in order to explore its role in shrimp innate immunity. DsLvTcf and dsEGFP (control) were synthesized using the T7 RiboMAX Express RNAi System (Promega, USA; Cat. No. P1700), according to the manufacturer's instructions. dsRNA was diluted in PBS, and 100 mL of PBS containing 20 mg dsRNA was injected into shrimps twice after 24 h. Another 12 h later, shrimp of the two groups were injected with 1×10 5 WSSV virions. Shrimp hemocytes (n = 8) were collected at 48 h postinfection (hpi). The transcriptional levels of LvTcf and the number of WSSV copies were then analyzed. siRNA was used to reduce the expression of endogenous b-catenin in S2 cells. SiDmb-catenin was synthesized by GenePharma based on the following sequences: (5′-3′) GCUUGCAAAUUCUGGCCUAT and UAGGCCAGAAUUUGCAAGCTT.

qRT-PCR
qRT-PCR was performed using TB Green Premix Ex Taq (Cat. No. RR820) in a Rotor-Gene ™ 6000 (Corbett Life Science) with the following program: 1 cycle of pre-denaturation for 1 min at 95°C, followed by 40 cycles of 95°C for 10 s, 56°C for 15 s, and 72°C for 15 s. Primers are listed in Table S1. We used LvEF-1a (GenBank accession No. GU136229) as an internal control, and each sample was analyzed in triplicate. Relative expression was determined via the 2 -DDCt method. Statistical significance was set at p<0.05.

Absolute q-PCR
We performed absolute q-PCR to monitor viral loads in shrimp. Briefly, we collected gills from shrimp at 48 hpi (n = 8 in the LvTcf knockdown experiment). Gill genomic DNA was extracted as described above. Primers for WSSV genomic DNA-F and WSSV genomic DNA-R (Table S1) were used to measure WSSV genomic copies via absolute q-PCR according to a previously described method (43). The WSSV copy number in 1 mg of shrimp genomic DNA was then calculated.

Plasmid Construction
GST protein was expressed via the pGEX-4T-2 vector (stored in our laboratory). The ORF of WSV083 was cloned into pGEX-4T-2 to express the WSV083-GST fusion protein. The ORFs of LvTcf and Lvb-catenin were cloned into the pIEx-4 vector (Novagen), with a c-Myc tag or FLAG tag fused to the Nterminus. The LvTcf catenin-binding domain deletion mutant (LvTcf57-556) was inserted into pIEx-4 with a c-Myc tag fused to the N-terminus. EGFP with a c-Myc, V5, or FLAG tag fused to the N-terminus was cloned into pIEx-4 vector at the same time. The ORFs of WSV056, WSV069 (IE1), WSV079, WSV100, WSV249, WSV403, wild-type (WT) WSV083, kinase domain deletion mutant WSV083DM, and kinase domain point mutant D459A WSV083PM were introduced into a pIEx-4 vector with a V5 tag fused to the N-terminus. FLAG-tagged ubiquitin was cloned into the pIEx-4 vector. The point mutations of LvTcf, including LvTcf2MuA (T39AT104A), LvTcf4MuA (T39AT10 4AT311AS315A), and LvTcf5MuA (T39AT104AT311AS315AS 356A) were introduced into the wild-type LvTcf expression plasmid using the Mut Express ® II Fast Mutagenesis Kit V2 (Vazyme, Nanjing, China; Cat. No. C214). The promoters of LvVago1-5 were cloned into the pGL3-Basic vector (stored in our laboratory). No. 17-5280-04) to remove nonspecific protein binding for 1 h at 4°C. Lysates were then immunoprecipitated with Sepharose and the indicated antibodies for at least 4 h. The gel or beads were washed sequentially with lysis buffer five times and boiled in 5×SDS loading buffer (Solarbio, Beijing, China; Cat. No. P1040) for western blot analysis. The protein samples were separated on SDS-PAGE gel and transferred to a nitrocellulose blotting membrane (Millipore, USA; Cat. No. IPVH00010). The membrane was blocked in 5% (w/v) skim milk in TBST (20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) at room temperature for 1 h. The membrane was then incubated with a primary antibody for 1 h at room temperature. A horseradish peroxidase (HRP)-conjugated secondary antibody was then incubated with the membrane for 1 h at room temperature. SuperSignalTM West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, USA; Cat. No. 34578) was used for signal detection.

GST Pull-Down
A GST or GST-WSV083 expression plasmid was transformed into Escherichia coli BL21, and single colonies were selected on plates supplemented with ampicillin (100 mg/mL). E. coli were then cultured and induced with 1 mM isopropyl-B-Dthiogalactopyranoside (Thermo Fisher Scientific, USA; Cat. No. R0392) for 16 h at 16°C in LB medium during the mid-exponential growth phase. Cells were harvested, sonicated, and protein was solubilized in PBS. Solubilized proteins were incubated with GST-Sepharose beads (GE Healthcare, USA; Cat. No. 17-5132-01) with rotation for 1 h at 4°C, collected by centrifugation, and washed five times with PBS. To determine whether the GST-WSV083 fusion protein binds to LvTcf, a pIEx-4-LvTcf-myc plasmid was transfected into High Five cells and harvested after 48 h, as mentioned above. The cell lysate was incubated with GST or GST-WSV083 fusion protein-bound beads with rotation at 4°C for 9 h, followed by five washes with PBS. Bound proteins were eluted in SDS loading buffer for Coomassie blue staining or western blotting.

Immunofluorescence Assay
Sf9 cells were transfected with the target plasmids and cultured for 24 h at 27°C in 35-mm dishes (Cellvis, USA; Cat. No. D35-20-1-N). The cells were washed twice with PBS, fixed with 4% paraformaldehyde at room temperature for 20 min, and were then permeabilized with 0.5% TritonX-100 (diluted in PBS) for 1 min. After washing three times, cells were blocked with 5% bovine serum albumin (diluted in TBST) for 1 h, followed by incubation for 1.

Calf Intestinal Alkaline Phosphatase (CIAP) Assay
LvTcf was co-expressed with EGFP, WSV083, or its mutants in High Five cells for 48 h. A spot of the cell lysate was used as input. The remaining proteins were immunoprecipitated with an anti-myc antibody and washed with lysis buffer three times as well as with phosphatase buffer (50 mM Tris-HCl, 0.1 mM EDTA, pH 8.5) three times. The bound beads in 30 ml phosphatase buffer were treated with CIAP (Thermo Fisher Scientific, USA; Cat. No. 18009-019) at 37°C for 1 h. The reaction was stopped with SDS loading buffer, and the beads were boiled for 5 min for western blotting.

Ubiquitination Assay
Ubiquitination assays were performed in High Five cells. Expression plasmids, including ubiquitin, were co-transfected into cells. After 12 h, the cells were treated with 10 mM protease inhibitor MG132 (Merck, USA; Cat. No. 474790) for 24 h and harvested with Western and IP cell lysis buffer [20 mM Tris (pH7.5), 150 mM NaCl, 1% Triton X-100] (Beyotime, Nanjing, C h i n a ; C a t . N o . P 0 0 1 3 ) . T h e s u p e r n a t a n t s w e r e immunoprecipitated as described above. The input and immunoprecipitates were subjected to western blot analysis.

Mass Spectrum Analysis
FLAG-LvTcf samples were collected from High Five cells coexpressing WSV083WT or WSV083DM. After SDS-PAGE analysis, FLAG-LvTcf bands were recovered for mass spectrometry analysis by Novogene (Beijing, China).

Effects of LvTcf on WSSV Infection
We obtained full-length LvTcf from the Litopenaeus vannamei transcriptome analyzed in our laboratory (unpublished). The LvTcf gene was 2220 bp in length (GenBank accession No. MT241372), which included a 1671 bp ORF encoding 556amino acid protein with a predicted molecular mass of 60.214 kDa. A catenin-binding domain, a conserved High Mobility group (HMG) domain, and a C-clamp domain were found at positions 1-56 aa, 288-358 aa, and 381-411 aa respectively ( Figures S1A, B). Multiple sequence alignment suggested that the LvTcf HMG domain had several highly-conserved Ser/Thr/ Lys phosphorylation sites ( Figure S1C). Phylogenetic analysis revealed that LvTcf was evolutionarily associated with arthropods, including crustaceans and insects ( Figure S1D).
To investigate the relationship between LvTcf and WSSV infection, we performed time-course analysis of LvTcf expression in hemocytes. The expression of viral gene ie1 was used as an index of WSSV infection level, increasing gradually, which suggested successful infection ( Figure 1A). LvTcf expression was significantly upregulated approximately 6-fold at 24 hpi and 11-fold at 48 hpi ( Figure 1B). These results indicated that WSSV infection positively regulated LvTcf expression. Further, we employed RNAi to knock down the expression of LvTcf in order to investigate its role during WSSV infection. Healthy shrimps were injected with dsLvTcf or dsEGFP (as control). Both groups were subsequently challenged with WSSV. 48 h after the injection of shrimp with WSSV, gill LvTcf was suppressed at the mRNA level compared to the control group ( Figure 1C). The number WSSV copies in gills increased after LvTcf silencing ( Figure 1D). Taken together, these results suggested that LvTcf plays a protective role against WSSV infection.
LvTcf Promotes the Production of LvVago1 Independently of Lvb-Catenin b-catenin and TCF usually form a complex in order to activate gene expression (37,44,45). As we predicted that the b-cateninbinding domain is located in the N-terminus of LvTcf, we sought to determine whether Lvb-catenin could bind to LvTcf. Co-IP experiments indicated that Lvb-catenin interacted with LvTcf in High Five cells (Figures 2A, B). Moreover, immunofluorescence analysis further revealed a strong colocalization of Lvb-catenin with wild-type LvTcf, but not with its catenin-binding domain deletion mutation (LvTcf57-556) ( Figure 2C). Lvb-catenin expressed alone was mainly localized in the cytoplasm, while LvTcf, a nuclear protein, was present in the nucleus. When Lvb-catenin and LvTcf were co-expressed in cells, Lvb-catenin translocated into the nucleus and colocalized with LvTcf ( Figure 2C).
According to previous reports, the b-catenin/TCF pathway facilitates interferon production following viral infection (30)(31)(32). Thus, we hypothesized that the Lvb-catenin/LvTcf pathway could promote the expression of shrimp interferon-like protein Vago during WSSV infection. As five isoforms of Vago have been identified in shrimp to date (46,47), we performed dual luciferase reporter gene assays in Drosophila S2 cells to confirm the Lvb-catenin/LvTcf-mediated transcriptional regulation at Vago promoters. To our surprise, the results indicated that LvTcf, but not Lvb-catenin, could significantly promote LvVago1 promoter activity, and Lvb-catenin/LvTcf together had a less effect ( Figures 2D). Further, knockdown of LvTcf led to decreased LvVago1 expression after WSSV infection ( Figures 2E, F). We found that LvTcf57-556, a catenin-binding domain deletion mutant, could still activate the LvVago1 promoter ( Figure 2G). This activation was not reduced under Lvb-catenin co-expression ( Figure 2H). To determine the influence of endogenous b-catenin in S2 cells, we silenced the expression of b-catenin via siRNA and detected the effect of LvTcf on the LvVago1 promoter. The knockdown of endogenous Dmb-catenin did not affect LvVago1 promoter activity, which was regulated by LvTcf ( Figure 2I). Based on the above-described results, we suggest that LvTcf activates the expression of LvVago1 independently of Lvbcatenin. We further detected the regulatory activity of LvTcf on the LvVago1 promoter upon WSSV stimulation. pGL3-LvVago1 was co-transfected with an empty vector or LvTcf expression plasmids in Drosophila S2 cells. WSSV was added at 36 and 42 h after transfection. After culturing for 48 h, the cells were lysed, and fluorescence activity was detected. WSSV stimulation increased LvVago1 promoter activity, which was regulated by LvTcf ( Figure 2J). Taken together, these results revealed that LvTcf could promote the expression of interferonlike protein LvVago1 independently of Lvb-catenin, thus having a protective role against WSSV infection.

LvTcf Interacts With WSV083
TCF is the main downstream nuclear transcription factor of the Wnt pathway and is involved in immune regulation. It is usually a target of diverse viruses (48). To reveal the regulatory effect of WSSV on host LvTcf, we sought to identify LvTcf-associated viral proteins. As key viral regulatory factors, WSSV immediate-early proteins play important roles in successful infection and replication. In addition, these proteins mainly localize to the host cell nucleus. Therefore, we selected WSSV immediate-early proteins with known functions to study their relationship with LvTcf. In High Five cells, viral immediate-early protein expression plasmids of WSV403, WSV249, WSV079, WSV083, WSV069, WSV100, and WSV056 were co-transfected with the LvTcf-myc expression plasmid. The results indicated that LvTcf may interact with WSV083 and WSV069 ( Figure S2). Transient transfection and Co-IP further indicated that WSV083 interacted with LvTcf ( Figure 3A). Moreover, WSV083-GST was expressed in E. coli BL21, while LvTcf-myc was expressed in High Hive cells due to its difficult expression in prokaryotic cells. A GST-pull-down was then performed, indicating that WSV083-GST, but not GST alone, could bind to LvTcf in vitro (Figures 3B, C). These results revealed that WSV083 interacted with LvTcf directly.

WSV083 Phosphorylates LvTcf
While exploring the interaction between LvTcf and WSV083, we found that LvTcf displayed an upshift in mobility on the SDS- PAGE gel when co-expressed with WSV083 ( Figure 3A). As WSV083 is essentially a serine/threonine protein kinase, we hypothesized that this phenomenon was caused by the WSV083-mediated phosphorylation of LvTcf. At the same time, WSV083 kinase domain deletion mutant WSV083DM and kinase domain ATP-binding point mutant D459A WSV083PM expression plasmids were constructed ( Figure 4A). We then co-expressed both LvTcf and WSV083 or its mutants in High Five cells. The LvTcf immunoprecipitated via anti-myc agarose was subjected to treatment with or without CIAP. The results revealed that LvTcf co-expressed with WSV083WT exhibited slower mobility through the gel compared to LvTcf co-expressed with the control vector. WSV083DM nor WSV083PM affected the mobility of LvTcf ( Figure 4B). After CIAP treatment, the mobility difference of LvTcf disappeared ( Figure 4B), indicating that WSV083 phosphorylated LvTcf.

WSV083 Increases the Degradation of LvTcf Through the Ubiquitin-Proteasome Pathway
In addition, we found that LvTcf protein levels were reduced in the presence of WSV083WT (Figures 3A, 4B). To validate this observation, we transfected High Five cells with equal amounts of LvTcf plasmid and increasing amounts of WSV083WT plasmid, while decreasing amounts of EGFP plasmid were added to maintain the same amount of transfection. Western blotting results indicated that WSV083 decreased the level of LvTcf in a dose-dependent manner ( Figure 5A). To further confirm whether WSV083 promoted the degradation of LvTcf through the proteasome pathway after its phosphorylation, we co-transfected LvTcf with pIEx-4, WSV083WT, WSV083DM, and WSV083PM expression plasmids into High Five cells. The proteasome inhibitor MG132 was used to block protein degradation. MG132 completely inhibited LvTcf degradation by WSV083WT, while the empty vector, WSV083DM, or WSV083PM, had no effect on LvTcf ( Figure 5B). This suggests that phosphorylation of LvTcf by functional WSV083 is necessary for its proteasome-mediated degradation. Since protein degradation is usually related to ubiquitination, we further determined whether LvTcf underwent ubiquitylation as a result of WSV083-mediated phosphorylation. LvTcf was cotransfected with EGFP, WSV083, and ubiquitin into High Five cells for intracellular ubiquitination assays. In the presence of WSV083WT, which possesses kinase activity, a polyUb-LvTcf smear of greater molecular mass was observed compared to the control group ( Figure 5C), indicating that WSV083 promoted the ubiquitylation of LvTcf. Moreover, immunofluorescence analysis indicated that LvTcf colocalized with WSV083, and the latter could change the subcellular localization of LvTcf upon addition of MG132 ( Figure 5D). We believe that WSV083-phosphorylated LvTcf was transferred to the cytoplasm for degradation. The current results suggested that WSV083 increased the degradation of LvTcf via the ubiquitinproteasome pathway. In addition, WSV083 inhibited the LvTcfmediated activity of the LvVago1 promoter ( Figure 5E). While our data cannot rule out an indirect mechanism where WSV083 regulates a kinase that in turn directly phosphorylates LvTCF, we favor a model where WSV083 phosphorylates LvTCF directly, on multiple residues that remain to be identified, leading to its degradation by the ubiquitin-proteasome pathway.

Identification of WSV083-Targeted LvTcf Phosphorylation Sites
Based on the results described above, we speculated that an E3 ligase might recognize the specific LvTcf residues that were phosphorylated by WSV083. To locate these, we employed mass spectrometry in LvTcf samples gathered from High Five cells co-expressing LvTcf with WSV083WT or WSV083DM ( Figure S3). The Thr39 and Thr104 LvTcf residues were found to be phosphorylated in cells co-transfected with WSV083WT, but not WSV083DM (Figures 6A, B). We therefore suggest that WSV083 phosphorylates LvTcf at Thr39 and Thr104. However, the LvTcfT39AT104A mutant (LvTcf2MuA) retained a mobility shift in SDS-PAGE gel when co-expressed with WSV083WT ( Figure 6C). LvTcf might therefore contain other residues that are phosphorylated by WSV083, in addition to Thr39 and Thr104. Thus, we continued to substitute some potentially conserved phosphorylated serine or threonine residues (Thr311, Ser315, and Ser356) with alanine. When T39, T104, Thr311, Ser315 and Ser356 (LvTcf5MuA) were simultaneously mutated, a mobility shift still occurred ( Figure 6D). CIAP treatment abolished the mobility difference of LvTcf mutants ( Figure 6E). These results indicated that a series of serine or threonine sites of LvTcf, including T39 and T104, could be phosphorylated by WSV083.

DISCUSSION
TCF, the key transcription factor of the Wnt signaling pathway, drives the development of lymphocytes (T cells, B cells), NK cells, and innate lymphoid cells (49,50). Further, TCF-1 is essential for both the initiation of TFH differentiation and the effector function of differentiated TFH during acute viral infection by promoting the expression of Bcl-6 (28). In addition, the b-catenin/TCF signaling pathway functions in innate defense mechanisms to defend against diverse pathogens via phagocytosis, autophagy, reactive oxygen species production, and antimicrobial peptide production (31).
In the current study, we demonstrated for the first time that LvTcf exerts antiviral effects by promoting the production of interferonlike protein LvVago1 independently of Lvb-catenin. Further, this antiviral effect was suppressed by WSSV protein kinase WSV083. WSSV triggered expression changes in diverse host molecules, including LvTcf. As shown in Figure 1, LvTcf mRNA was significantly increased following WSSV infection ( Figures 1A, B), indicative of a close relationship between LvTcf and WSSV infection. RNAi experiments revealed that LvTcf knockdown resulted in greater WSSV proliferation ( Figures 1C, D), suggesting that the former plays a protective role against WSSV infection. A recent research found that two isoforms of LvPangolin, LvPangolin1 (LvTcf in this work) and LvPangolin2, had antiviral properties against WSSV, which is compatible with our findings (55). Wnt/b-catenin/TCF signaling pathway-mediated responses to bacterial and viral infections have been the subject of extensive study, highlighting the cascade's roles in orchestrating phagocytosis, antimicrobial or interferon defense, as well as inflammatory cytokine production (56)(57)(58). In the current study, we sought to explore the LvTcf-mediated regulation of downstream immune-related genes in shrimp. Interferons, a family of immune proteins which spearhead antiviral defense, are regulated by b-catenin/TCF in an interferon regulatory factor-independent manner (59). There are five interferon-like proteins in shrimp. Among them, the interferon regulatory factor-mediated antiviral effects of LvVago1, LvVago4, and LvVago5, have been previously characterized (47,60). Sequence analysis revealed that LvTcf harbors several possible binding sites in the promoters of LvVago-encoding genes. LvTcf promoted the activity of the LvVago1 promoter during WSSV infection ( Figures 2D-F, H, J). In a recent study, b-catenin was shown to activate gene expression by binding to other transcription factors such as FOXO4 without TCF (61). We found that LvTcf initiated the production of LvVago1 independently Lvb-catenin even though they could interact with each other. Moreover, cooperation with Lvb-catenin reduced the effect of LvTcf on LvVago1 promoter activity. It is suggested that LvTcf acts by itself or cooperates with other transcription factors to activate LvVago1 expression, whereas the Lvb-catenin/LvTcf complex might upregulate the transcription of other shrimp target genes. Taken together, our study clarified one of the mechanisms through which LvTcf defends against WSSV infection.
Host immune molecules are often targeted by viruses. The regulation of post-translational modifications, especially the ubiquitination of host immune molecules, is an effective means of immune evasion (20,62). For instance, IpaH9.8, an E3 ubiquitin ligase of Shigella flexneri, could target human GBP-1 to induce its ubiquitination and degradation, thus suppressing host defense (63). Kaposi's sarcoma-associated herpesvirus encodes an E3 ligase RTA that suppresses innate immunity via the ubiquitin-mediated degradation of MyD88 (64). As pivotal nuclear co-activators of the Wnt signaling pathway, TCFs are regulated by various factors. LEF1, TCF7L1, and TCF7L2 are phosphorylated by HIPK2 at their HMG box domain (65). These are also phosphorylated by NLK, as the first step for ubiquitylation by NARF, and are subsequently degraded via the proteasome (66). Various reports have indicated that SUMOylation and acetylation of TCFs regulate their subcellular localization and transcriptional activity (67,68). In the present study, we discovered that LvTcf was modulated by the viral Ser/Thr protein kinase WSV083. WSV083 caused the phosphorylation of LvTcf and delayed its mobility in SDS-PAGE due to changes in molecular weight and isoelectric point ( Figure 4B). WSV083-mediated LvTcf phosphorylation could impact the latter's protein stability ( Figures 5A-C). Phosphorylated LvTcf was transferred into the cytoplasm for degradation via the ubiquitin-proteasome pathway ( Figure 5D). Some E3 ligases mediate the degradation of target proteins necessary for the recognition of specific phosphorylated amino acid residues (69)(70)(71). We speculated that an E3 ligase might recognize the LvTcf residues phosphorylated by WSV083 prior to its ubiquitination. However, mass spectrometry analysis did not identify all LvTcf residues targeted by WSV083 ( Figure 6). In addition, although over-expression of WSV083 downregulated LvTcf protein and inhibited its ability to activate LvVago1 promoter ( Figure 5E), this did not contradict the findings in Figure 2J that WSSV infection induced LvTcf activation of the LvVago1. In contrast to WSV083 overexpression, the amount of WSV083 in S2 cells was insufficient to cause LvTcf breakdown in a WSSV-stimulated manner. S2 cells, on the other hand, were capable of receiving and responding to WSSV infection.
In response to WSSV stimulation, LvTcf in S2 cells was likely to upregulate the activity of the LvVago1 promoter.
In summary, our work showed that LvTcf promoted the expression of shrimp interferon-like protein Vago1 following WSSV infection, whereas WSSV protein kinase WSV083 could directly bind to and phosphorylate LvTcf, promoting its degradation via the ubiquitin-proteasome pathway (Figure 7). The current findings provide new insight into the response against WSSV, thus having implications for disease control.

DATA AVAILABILITY STATEMENT
The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material.

AUTHOR CONTRIBUTIONS
CW, LR, and HS designed the experiments. CW, WL, LL, and SL performed the experiments and data analysis. CW and LR were FIGURE 7 | Model of the relationship between LvTcf and WSSV infection. WSSV infection (pathogenic stress) induced the LvTcf-driven production of interferon-like protein LvVago1 independently of Lvb-catenin, as an antiviral defense mechanism. However, WSSV encodes a serine/threonine protein kinase, WSV083, which phosphorylates LvTcf. Phosphorylated LvTcf is translocated to the cytoplasm, recognized by an E3 ligase, and degraded via the ubiquitin-proteasome pathway. responsible for writing, finalizing, and submitting the manuscript. All the authors discussed the results. All authors contributed to the article and approved the submitted version.