How immune breakthroughs could slow disease progression and improve prognosis in COVID-19 patients: a retrospective study

Background Previous infections and vaccinations have produced preexisting immunity, which differs from primary infection in the organism immune response and may lead to different disease severities and prognoses when reinfected. Objectives The purpose of this retrospective cohort study was to investigate the impact of immune breakthroughs on disease progression and prognosis in patients with COVID-19. Methods A retrospective cohort study was conducted on 1513 COVID-19 patients in Chengdu Public Health Clinical Medical Center from January 2020 to November 2022. All patients were divided into the no immunity group (primary infection and unvaccinated, n=1102) and the immune breakthrough group (previous infection or vaccination, n=411). The immune breakthrough group was further divided into the natural immunity subgroup (n=73), the acquired immunity subgroup (n=322) and the mixed immunity subgroup (n=16). The differences in clinical and outcome data and T lymphocyte subsets and antibody levels between two groups or between three subgroups were compared by ANOVA, t test and chi-square test, and the relationship between T lymphocyte subsets and antibody levels and the disease progression and prognosis of COVID-19 patients was assessed by univariate analysis and logistic regression analysis. Results The total critical rate and the total mortality rate were 2.11% and 0.53%, respectively. The immune breakthrough rate was 27.16%. In the no immunity group, the critical rate and the mortality rate were all higher, and the coronavirus negative conversion time was longer than those in the immune breakthrough group. The differences in the critical rate and the coronavirus negative conversion time between the two groups were all statistically significant (3.72% vs. 0.24%, 14.17 vs. 11.90 days, all p<0.001). In addition, in the no immunity group, although lymphocyte counts and T subsets at admission were higher, all of them decreased consistently and significantly and were significantly lower than those in the immune breakthrough group at the same time from the first week to the fourth week after admission (all p<0.01). The total antibody levels and specific Immunoglobulin G (IgG) levels increased gradually and were always significantly lower than those in the immune breakthrough group at the same time from admission to the fourth week after admission (all p<0.001). Moreover, in the natural immunity subgroup, lymphocyte counts and T subsets at admission were the highest, and total antibody levels and specific IgG levels at admission were the lowest. Then, all of them decreased significantly and were the lowest among the three subgroups at the same time from admission to one month after admission (total antibody: from 546.07 to 158.89, IgG: from 6.00 to 3.95) (all p<0.001). Those in the mixed immunity subgroup were followed by those in the acquired immunity subgroup. While lymphocyte counts and T subsets in these two subgroups and total antibody levels (from 830.84 to 1008.21) and specific IgG levels (from 6.23 to 7.51) in the acquired immunity subgroup increased gradually, total antibody levels (from 1100.82 to 908.58) and specific IgG levels (from 7.14 to 6.58) in the mixed immunity subgroup decreased gradually. Furthermore, T lymphocyte subsets and antibody levels were negatively related to disease severity, prognosis and coronavirus negative conversion time. The total antibody, specific IgM and IgG levels showed good utility for predicting critical COVID-19 patients and dead COVID-19 patients. Conclusion Among patients with COVID-19 patients, immune breakthroughs resulting from previous infection or vaccination, could decelerate disease progression and enhance prognosis by expediting host cellular and humoral immunity to accelerate virus clearance, especially in individuals who have been vaccinated and previously infected. Clinical trial registry Chinese Clinical Trial Register ChiCTR2000034563.

in the immune breakthrough group at the same time from the first week to the fourth week after admission (all p<0.01).The total antibody levels and specific Immunoglobulin G (IgG) levels increased gradually and were always significantly lower than those in the immune breakthrough group at the same time from admission to the fourth week after admission (all p<0.001).Moreover, in the natural immunity subgroup, lymphocyte counts and T subsets at admission were the highest, and total antibody levels and specific IgG levels at admission were the lowest.Then, all of them decreased significantly and were the lowest among the three subgroups at the same time from admission to one month after admission (total antibody: from 546.07 to 158.89, IgG: from 6.00 to 3.95) (all p<0.001).Those in the mixed immunity subgroup were followed by those in the acquired immunity subgroup.While lymphocyte counts and T subsets in these two subgroups and total antibody levels (from 830.84 to 1008.21) and specific IgG levels (from 6.23 to 7.51) in the acquired immunity subgroup increased gradually, total antibody levels (from 1100.82 to 908.58) and specific IgG levels (from 7.14 to 6.58) in the mixed immunity subgroup decreased gradually.Furthermore, T lymphocyte subsets and antibody levels were negatively related to disease severity, prognosis and coronavirus negative conversion time.The total antibody, specific IgM and IgG levels showed good utility for predicting

Introduction
Since mid-December 2019, the COVID-19 pandemic caused by SARS-CoV-2 has been ongoing and evolved into a major global health threat (1).By April 2023, over 762 million confirmed cases and over 6.8 million deaths have been reported globally (2).SARS-CoV-2 elicits both innate and adaptive immune responses, including the development of specific T cells and antibodies.Efficient immune responses are indispensable for the regulation and eradication of pathogen infections (3).
The adaptive immune system consists of three lymphocyte types: B cells, CD3+CD4+ T cells, and CD3+CD8+ T cells (4).Tlymphocytes and immune antibodies are necessary to control viral infections.Similar to severe influenza and other respiratory viral infections, lymphopenia is frequently observed in COVID-19 and exhibits a positive correlation with the clinical disease severity (5,6).Adaptive responses of immune antibodies provide the first line of defense during viral infections and are important for long-term immunity and immune memory (7).Therefore, the number of T cells and antibodies can be diagnostic and predictive factors for identifying patients who will have severe disease (6,8).
The topic of pre-existing immunity to SARS-CoV-2 infection, acquired through natural infection or vaccination, has gained significant attention currently (9).Several countries have also reported cases of breakthrough infections among individuals who were vaccinated or had a previous infection (9)(10)(11).Studies have shown that the maintenance of IgG and T-cell responses persists in most patients for at least 3-4 months following infection (12) and even more than 13 months (13).Vaccination is considered key to reducing the risk of SARS-CoV-2 infection, severe illness, and mortality risks (14).The immune breakthrough is speculated to be attributed to factors including high viral load exposure, infection with a different viral strain, and antibody-dependent enhancement (10).In the post-COVID-19 era, understanding the potential influence of immune breakthrough is crucial to improving COVID-19 prevention and control measures.Therefore, we conducted a population-based study to investigate the presence of SARS-CoV-2 T lymphocytes and antibody levels, carefully examining the fluctuations in these levels among patients with varying immune statuses.

Subjects
This was a retrospective cohort study.All 1,513 patients with COVID-19 from the hospital isolation ward who presented to the Public Health Clinical Centre of Chengdu from January 16, 2020, to September 30, 2022, were retrospectively recruited (Figure 1, Table 1).The study was approved by the Public and Health Clinical Centre of Chengdu Ethics Committee (ethics approval number: PJ-K2020-26-01).Written informed consent was waived by the Ethics Commission of the designated hospital because this study was related to emerging infectious diseases.

Inclusion and exclusion criteria
The inclusion criteria were as follows: no sex limit; age ≥18 years old; COVID-19; and inpatient isolation and treatment time >1 day.
The exclusion criteria were as follows: age<18 years old and isolation and treatment time <1 day.The typing criteria were as follows: (1) asymptomatic infection indicated that there were no clinical symptoms and no pneumonia manifestations on imaging; (2) the light type indicated that the clinical symptoms were mild, and there were no pneumonia manifestations on imaging; (3) the common type indicated that the clinical symptoms included fever and respiratory tract, and pneumonia could be seen on imaging; (4) the severe type indicated that the patients had any of the following criteria: respiratory distress, RR≥30 times/min; in the resting state, oxygen saturation ≤ 93%; arterial blood oxygen partial pressure (PaO2)/oxygen concentration (FiO2)≤300mmHg (1mmHg=0.133kPa),living in areas with high altitude (over 1000 meters above sea level), and PaO2/FiO2 should be corrected according to the following formula: PaO2/FiO2*[atmospheric pressure(mmHg)/760]; pulmonary imaging showed that lesions with significant progress over 50% within 24-48h were managed as heavy; (5) the critical illness type criteria included one of the following conditions: respiratory failure occurs and mechanical ventilation is needed; Shock occurs; and combining other organ failure requires intensive care units (ICU) monitoring.

Disease diagnosis, clinical typing, cure criteria and laboratory testing
The cured discharge standard was as follows: the body temperature returned to normal for more than 3 days; respiratory symptoms improved significantly; lung imaging showed a significant improvement in acute exudative lesions; and two consecutive sputum, nasopharyngeal swabs and other respiratory specimens tested negative for nucleic acid (sampling time at least 24 h apart).
The relevant serological assays were performed by Enzyme-Linked Immunosorbent Assay (ELISA) in the laboratory of the hospital.

Grouping standards
Among the 1,513 COVID-19 cases, 1102 and 411 cases were divided into the no immunity group (primary infection and no vaccination) and the immune breakthrough group (previous infection or vaccination), respectively (Figure 1, Table 1).

FIGURE 1
Patient data (n=1513).Non critical refers to the clinical type of COVID-19 that is asymptomatic, light and common.Critical refers to the clinical type of COVID-19 that is associated with severe and critical illness.No immunity refers to primary infection and no vaccination.Immune breakthrough refers to previous infection or vaccination.Natural immunity refers to previous infection.Acquired immunity refers to vaccination.Mixed immunity refers to previous infection and vaccination.
Among the 411 immune breakthrough cases, 73 patients with previous infection were assigned to the natural immunity group, 322 patients who had been vaccinated (no distinction was made between vaccine type and dose) were assigned to the acquired immunity group, and 16 patients who had both previous infection and been vaccinated were assigned to the mixed immunity group (Figure 1, Table 1).
Among the 1,513 COVID-19 cases, 1,471 noncritical patients (patients with asymptomatic infection, with light and with common clinical type) were assigned to the noncritical group, and 42 critical patients (patients with severe and with critical illness clinical type) were assigned to the critical group (Figure 1, Table 1).
Among the 1,513 COVID-19 cases, 1505 surviving patients were assigned to the survival group, and 8 dead patients were assigned to the death group (Figure 1, Table 1).

Definition of the viral negative conversion time, disease severity and prognosis
The disease severity included critical illness (COVID-19 patients with severe or critical illness clinical type) and noncritical illness (COVID-19 patients with asymptomatic infection, light or common clinical type).The prognosis included death and survival within four weeks after admission.The coronavirus negative conversion time was the time from onset to the first negative nucleic acid test meeting the discharge criteria.

Data collection
The data were collected from a subset of patients treated at Chengdu Public Health Clinical Medical Center from January 2020 to November 2022.All data of 1,513 cases, including clinical data, laboratory data and demographic data, were collected to establish databases.Researchers strictly controlled the accuracy, completeness and authenticity of all data.

Statistical analysis
SPSS 26.0 (SPSS, Chicago, IL, USA) and GraphPad Prism 8 (GraphPad, CA, USA) were used for statistical analyses.Measurement data with a normal distribution are presented as the mean and standard deviation, and measurement data with a nonnormal distribution are presented as the median and interquartile range (IQR).The categorical data are expressed as a percentage or proportion.Data with a normal distribution and homogeneity of variance between multiple groups were compared using one-way or two-way ANOVA, and further comparison between two groups was performed using the least significant difference (LSD) t test.Data with a normal distribution and homogeneity of variance between two groups were compared using the independent samples t test.Enumeration data are presented as percentages or proportions, and data between two or multiple groups were compared using a chi-square test.Analysis of influencing factors of disease severity and prognosis was performed using binary logistic regression analysis.Receiver operating characteristic (ROC) analysis was used to assess lymphocytes and subsets to distinguish non critical from severe COVID-19 patients.P<0.05 was considered statistically significant.

Patient and public involvement
Patients and the public were not involved in the development of the research questions or in the design of the study.Patients received verbal and written information about the study; however, they were not involved in the recruitment of subjects or the conduct of the study.Additionally, the burden of the intervention was assessed by the investigators.The participants were assessed for eligibility, and data collection was performed.Dissemination of the general results (without personally identifying data) will occur on demand.The Ethics Committee of the Public Health Clinical Centre of Chengdu approved this study (ethics approval number: PJ-K2020-26-01).Written informed consent was waived by the Ethics Commission of the designated hospital because this study is related to emerging infectious diseases.

Baseline conditions (characteristics of the study population)
A total of 1,513 patients with COVID-19 were included in this study.Their demographic and clinical characteristics are listed in Table 1.The median age of all patients included in the study was 35 years, and males accounted for the majority (71.0%).The median coronavirus negative conversion time was 11.0 days, and the duration of hospitalization was 15.0 days.

Comparisons between the immune breakthrough group and the no immunity group
In the immune breakthrough group, the proportion of domestically transmitted cases was significantly higher than that in the no immunity group (Table 2) (p<0.05), while the critical illness rate was significantly lower than that in the no immunity group (Table 2) (0.24% vs. 3.72%, p<0.05).The mortality rate was slightly lower than that in the no immunity group, although the difference was not statistically significant (Table 2) (p=0.083).
The age (Figure 2A) was slightly younger than that in the no immunity group (p<0.05), and there was no significant difference in BMI (Figure 2B) or duration of hospitalization (Figure 2C) between the two groups (all p>0.05).However, in the immune breakthrough group, the coronavirus negative conversion time (Figure 2D) was significantly shorter than that in the no immunity group (p<0.001).
In addition, in the no immunity group, CD3+ counts, CD3 +CD4+ counts, CD3+CD8+ counts and lymphocyte counts at admission were higher than those in the immune breakthrough group (Figures 3A-C, E) (all p<0.05), but all of them then decreased to the lowest level at the first week and were always significantly lower than those in the immune breakthrough group from the first week to the fourth week (Figures 3A-C, E) (all p<0.01).The ratio of CD3+CD4+ to CD3+CD8+ cells between the two groups from onset to the fourth week after onset was always similar to each other (Figure 3D) (p>0.01).Moreover, in the no immunity group, total antibody levels and specific IgG levels from onset to the fourth week were always significantly lower than those in the immune breakthrough group (Figures 3F, H) (all p<0.001).In the no immunity group, the specific IgM levels were always lower than those in the immune breakthrough group from the first week to the fourth week, but a significant difference was found only at the first week after onset (Figure 3G).

Comparison of types of comorbidities between the immune breakthrough group and the no immunity group
Metabolic diseases such as fatty liver, diabetes, hypertension, hyperlipidemia, etc. were the most common in both groups, with no difference (p all <0.05) (Table 3).The incidence of cardiovascular disease was higher in the no immunity group (p<0.05)(Table 3).Besides, the incidence of hypoxemia (p<0.001) and cardiovascular disease (p<0.05) was significantly higher in the no immunity group than in the immune breakthrough group (Table 3).

Comparisons between the natural immunity subgroup, the acquired immunity subgroup and the mixed immunity subgroup
Compared to the natural immunity subgroup and the acquired immunity subgroup, age was slightly younger (p<0.05), and the duration of hospitalization was obviously shorter than that in the mixed immunity subgroup (p<0.01)(Table 4).The coronavirus negative conversion time in the mixed immunity subgroup was also slightly shorter than that in the other two groups, but the difference was not statistically significant (Table 4) (p=0.057).
Moreover, in the acquired immunity subgroup, CD3+ counts, CD3 +CD4+ counts, CD3+CD8+ counts and lymphocyte counts at admission were the highest and then decreased the lowest level at the first month among the three groups.All of them in the other two groups showed an increase from admission to the first month after admission, and those in the mixed immunity group reached their highest levels (Figures 4A-D).In the mixed immunity subgroup, the total antibody levels and specific IgG levels were the highest at admission and then decreased slightly over the following month ( Figures 4E, F).Those in the natural immunity subgroup were the lowest at admission, with a sharp decrease during the following month ( Figures 4E, F).Only those in the mixed immunity subgroup rose from second place on admission to first place at one month ( Figures 4E, F).

Univariate and multivariate analysis of baseline characteristics, lymphocyte subsets, and antibody levels for disease severity and prognosis
For critical cases, the age was significantly older, the number of comorbidities and the imported cases were higher (p all=0.000).Most T-lymphocyte subsets (except CD3+CD8+% and CD19+%) and all antibody levels (p all<0.05)were significantly higher in critical cases, whereas the level of CD56+% (p=0.024) was higher in non-critical cases (Table 5).Similarly, all the death cases were imported cases, with an average age of 77.5 years (p=0.000), a slightly higher proportion of females (p=0.034), an increased BMI index (p=0.013),and all combined with more than 3 diseases (p=0.000),without a history of previous infection.CD3+, CD3 +CD4+, CD3+CD8+, LY, LY%, CD19+ and all antibody levels in non-critical cases were higher (p all <0.01) (Table 5).
The influencing factors for disease severity by multiple linear regression analysis were age, lymphocyte percentage, source of cases and CD3+CD8+ counts (Table 6).Moreover, the influencing factors for prognosis were age, sex, and source of cases (Table 6).

The prediction of the antibody levels on disease severity and prognosis in patients with COVID-19
According to the ROC analysis, the total antibody, IgM and IgG levels showed good utility for predicting critical COVID-19 patients (Table 7).The areas under the curve of total antibody, IgM and IgG for disease severity were 0.854, 0.904 and 0.794, respectively (Table 7, Figure 5).The sensitivities were 98.10%, 76.40%, 96.10%, while the specificities were 75.00%, 100.00%, 75.00% (Table 7).

Discussion
In the post epidemic era, breakthrough infections of SARS-CoV-2 are being increasingly observed worldwide due to the high pervasiveness of viral spread, the emergence of novel variants (11), progressive ease of restrictive measures and waning protection against infection.In this retrospective study, we found that the critical illness rate was 2.8%, and the mortality rate was 0.5%, both of which were low, which was consistent with our previous report (16)(17)(18)(19).We also found that the immune breakthrough rate in this study cohort was 27.2%, with 4.8% of patients having previous infections, 22.4% of patients having been vaccinated, and only 6% of patients having been fully vaccinated.This finding align with previous reports indicating that 4.0% to 5.3% of fully vaccinated individuals exhibit immune dysfunction (20,21).A previous study reported that among fully vaccinated individuals, the incidence rate for COVID-19 breakthrough infection was 5.0 per 1000 personmonths (20).Compared with partial vaccination, the incidence rate for COVID-19 breakthrough infection in full vaccination was associated with a 28% reduced risk (20).The reinfection rate among patients with COVID-19 is estimated to range from 2.3% to 21.4%, as indicated by a meta-analysis ( 22).The reinfection rate in our study was also in the range.
We reported the differences in disease severity and prognosis and in T lymphocyte subsets and antibody levels between different immune statuses to investigate the impact of immune breakthroughs on disease progression and prognosis in patients with COVID-19.We found that patients with preexisting immunity by previous infection or vaccination had a lower severe rate and mortality rate than those with primary infection, with all fatalities occurring exclusively among patients without previous immune protection.The results align with the findings reported in the existing literature (23)(24)(25).Since the hypoxemia secondary to infection occurred in the no immunity group, the proportion of patients with chronic obstructive pulmonary disease and cardiovascular disease was also higher in the no immunity group, suggesting that the infection combined with cardiopulmonary system diseases may be the main factor for the development of severe illness.Many studies have reported that patients with preexisting immunity have milder illnesses and lower rates of hospitalization (23, 24).Immune breakthrough patients were significantly less likely to experience severe disease or death than matched unvaccinated patients (25).With only one severe case and no deaths in preexisting immunity patients, we were unable to analyze differences in severe and mortality rates according to route of immunization.However, it also implies that pre-existing immunity diminishes the probability of progressing to severe illness following reinfection.Similar to previous studies (3,6), we observed higher levels of Tlymphocytes and antibodies in non-critical cases.We also found that patients with preexisting immunity by previous infection or vaccination exhibited shorter coronavirus negative conversion times and higher T lymphocyte subsets and antibody levels.

SARS-CoV-2 infection is associated with lymphopenia, particularly in CD4+ T cells and CD8+ T cells (26), indicating abnormal immune function during SARS-CoV-2 infection (27).
Failure to generate a timely T-cell response during natural SARS-CoV-2 infection has been linked to the development of severe COVID-19 cases (4).The immune memory generated by vaccination or natural infection serves as a reservoir of protective immunity that can rapidly expand upon re-exposure to the virus, potentially limiting viral replication during the early stages of infection (28).In addition to mitigating the severity of infection, breakthrough infections are less infectious than primary infections (29).
Preexisting immunity is associated with four major components of immunological memory: memory CD4+ T cells, memory CD8+ T cells, antibodies, and memory B cells (4).Both SARS-CoV-2specific CD4+ and CD8+ memory T cells peaked within the initial   month of infection, followed by a gradual decline over the subsequent 6 to 7 months (30).The CD4+ and CD8+ T-cell responses exhibited comparable levels between mRNA vaccination and infection, with similar T-cell production observed at both 6 months after the second dose and 6 months post-infection (31).Patricia (32) et al. found that the peak T-cell response 2 weeks after full vaccination was comparable to the peak response in mild and moderate patients.However, varying vaccine types, doses and intervals resulted in different immune memory statuses (31).In a retrospective study from Israel, in individuals who received the Pfizer mRNA vaccine (never infected), higher initial antibody levels were followed by a more rapid decline than in those with SARS-CoV-2 virus infection (33).Failure to generate sufficient IgG antibodies is linked to reduced survival (34).In contrast to our findings, some studies showed higher levels of antibodies (IgM and IgG) in severe patients than in mild-to-moderate patients (35)(36)(37).These results indicated that, in addition to antiviral efficacy, antibody responses might be associated with secondary antibodymediated organ damage (36).Due to our subjects were mainly non critical patients, the lymphocyte and antibody levels of critical patients were not analyzed.
In addition, we further analyzed the different immune statuses of the three preexisting immune routes: natural immunity, acquired immunity and mixed immunity.The T-lymphocyte subsets exhibited the highest level one month after infection, surpassing that of the other two groups.Total antibody and IgG levels were the highest at admission and decreased slowly over the following month.Levels of anti-S1 IgG production were much higher in vaccinated individuals than in naturally infected individuals within three months of vaccination.Many studies have shown that vaccination induces a more robust and targeted immune response compared to the natural infection (32,38).Therefore, the optimal approach to combat COVID-19 is to enhance immune function through vaccination.However, many studies have shown that mixed immunization appears to confer a more robust protective effect (39,40).Our study also confirmed this view, with the shortest length of hospitalization and the shortest coronavirus negative conversion time results in the mixed immunization group (Table 4).A single    Further research found that there were significantly higher levels of antibodies in fully vaccinated individuals with natural immunity than in fully vaccinated individuals without prior infection.Vaccination after previous infection appeared to enhance and prolong immunity, even more than 1 year after the initial infection, with no sign of weakening (39).These studies suggest that in the post pandemic era, we can still fight the virus through a combination of natural immunity and vaccination.
The association between advanced age, a high number of comorbidities, and an unfavorable prognosis has been observed in previous study (16).The higher mortality rate observed in domestically transmitted cases is expected to be attributed to the limited extent of domestic transmission during the period of case collection.The large difference in the number of critical and noncritical patients (1471 vs 42) may have biased the results of the ROC analysis, but in combination with the results of the univariate analysis, it can be inferred that the antibody level is a reliable indicator of prognosis.Consistent with previous findings (5,6,8), critical and dead cases exhibited lower levels of T-lymphocyte subsets and antibodies, which served as reliable indicators for disease progression.
However, there are still some limitations of this study.Importantly, it was a single-center, retrospective study, and all the inherent limitations of retrospective studies are unavoidable.The incidence of critical cases, especially deaths, was minimal.Additionally, reinfected cases were extensively interviewed after a time interval, allowing the possibility of recall bias.Due to the information restrictions, the data related to the strains are in the CDC, which we cannot get.We can only infer from the time that the strains of our patients' infection range from the original strain SARS-CoV-2 to Delta variant (B.1.617.2) and omicron (B.1.1.529).In addition, vaccine types were not available and the sequencing of vaccination and infection was not distinguished, no further analysis could be conducted.

Conclusion
Currently, reported cases do not accurately represent the infection rate due to a global reduction in testing and reporting, but the World Health Organization still reports more than 1 million new positive cases every four weeks.Hence, our findings serve as a valuable reference for predicting disease progression and treating patients with COVID-19 by monitoring changes in lymphocyte subsets and antibodies.The immune breakthrough group had lower rates of critical disease and mortality compared to the no immunity group, while the mixed immunity group showed highest levels of Tlymphocyte subsets and antibodies.Therefore, vaccination should be intensified to enhance the protective effect even in the post pandemic era, when most people have already been infected.
Using characteristics of antibody levels for discriminating the critical cases from the noncritical patients (n=1513; critical and noncritical groups, n=42 and 1471, respectively).ROC analysis showing the performance of antibody levels in distinguishing critical cases from noncritical patients.ROC, receiver operating characteristic curve.
The criteria for COVID-19 clinical typing, disease diagnosis and cure were in accordance with the seventh Trial Version of the Novel Coronavirus Pneumonia Diagnosis and Treatment Guidance (15).The diagnosis criteria were cases with one of the following etiological pieces of evidence: real-time fluorescence reverse transcriptionpolymerase chain reaction (RT-PCR) detected the positive nucleic acid of the new coronavirus and sequencing of viral genes.

2
FIGURE 2 Comparison of age, BMI, duration of hospitalization and coronavirus negative conversion time between the immune breakthrough group and the no immunity group (n=1513; the immune breakthrough group and the no immunity group, n=411 and 1102, respectively).(A) Age.(B) BMI.(C) Duration of hospitalization (day).(D) The coronavirus negative conversion time (day).BMI, body mass index.Unpaired t tests were used for comparisons between two groups, ns p>0.05, *p<0.05,***p<0.001.
dose of vaccine elicited higher memory T and B-cell responses in previously infected individuals Due to the existence of immune memory, previously infected individuals produce more rapid and lasting cellular and humoral immunity after vaccination(42,43).
BMI, body mass index.

TABLE 2
Comparison of baseline conditions between the two groups (n=1513).

TABLE 3
Comparison of types of comorbidities between the two groups (n=1513).

TABLE 4
Comparison of baseline conditions between the three groups.

TABLE 5
Univariate analysis of disease severity and prognosis.

TABLE 6
Multiple stepwise regression analysis of influencing factors of disease severity and prognosis (n=95).

TABLE 7
The performance of various methods for distinguishing between critical cases and non-critical cases (n=1513).