Case report: Target and immunotherapy of a lung adenocarcinoma with enteric differentiation, EGFR mutation, and high microsatellite instability

Background Pulmonary enteric adenocarcinoma (PEAC) is a rare histological subtype of non-small-cell lung cancer (NSCLC) with a predominant (>50%) enteric differentiation component. The frequency of high microsatellite instability (MSI-H) is very low in lung cancer. EGFR tyrosine kinase inhibitors and immunotherapy are standard treatment for NSCLC patients, but their effectiveness in lung adenocarcinoma with pulmonary enteric differentiation is unknown. Case presentation This report describes a 66-year-old man who was initially diagnosed with metastatic lung adenocarcinoma with EGFR mutation based on pleural fluid. A lung biopsy was obtained after 17 months of first-line icotinib treatment. Histological analysis of biopsy samples and endoscopic examination resulted in a diagnosis of adenocarcinoma with enteric differentiation. Next-generation sequencing of 1,021 genes showed EGFR E19del, T790M, and MSI-H, while immunohistochemical assay showed proficient expression of mismatch repair (MMR) proteins. Consequently, the patient was treated with osimertinib and had a progression-free survival (PFS) of 3 months. His treatment was changed to chemotherapy with/without bevacizumab for 6.5 months. Then, the patient was treated with one cycle of camrelizumab monotherapy and camrelizumab plus chemotherapy, respectively. The tumor continued to grow, and the patient suffered pneumonia, pulmonary fungal infections, and increased hemoptysis. He received gefitinib and everolimus and died 2 months later and had an overall survival of 30 months. Conclusion In summary, our case describes a rare pulmonary enteric adenocarcinoma with an EGFR-activating mutation and MSI-H, responding to an EGFR tyrosine kinase inhibitor and poorly benefiting from an immune checkpoint inhibitor.


Introduction
Pulmonary enteric adenocarcinoma (PEAC) is a rare variant of lung adenocarcinoma.According to the 2015 World Health Organization (WHO) classification, PEAC has been defined as primary pulmonary adenocarcinoma with more than 50% of intestinal differentiation components, and the tumor cells should be positive for at least one immunohistochemical marker of enteric differentiation, including CK20, CDX2, and MUC2 (1).The pathogenesis of PEAC and specific treatment plans have not been fully determined.At present, the treatment strategy for PEAC is similar to that of lung adenocarcinoma.The strategy methods derived from the literature for PEAC are mainly surgery and chemotherapy.Although several case reports have described immunotherapies in patients with PEAC, few have reported targeted therapy (2).
KRAS and DNA mismatch repair (MMR) genes are more frequently mutated in PEAC compared with those in other lung adenocarcinomas (3,4).The high frequency of MMR mutation rates may facilitate the possibility of checkpoint-blocking immunotherapy for PEAC patients.The positive rate of EGFR mutations is approximately 16.7%, much lower than that of KRAS (3,4).Few reports to date have described the effects of treatment with sequential EGFR tyrosine kinase inhibitors in PEAC.The present report describes the targets and immunotherapy of a lung adenocarcinoma with enteric differentiation, EGFR-activating mutation, and high microsatellite instability (MSI-H).

Case report
In February 2019, a 66-year-old man visited the First Affiliated Hospital of Guangxi Medical University due to a 2-month history of cough, sputum, and shortness of breath.The chest computed tomography (CT) scan showed a suspicious central right lung mass, with hilum of right lung, bilateral lung, and mediastinal lymph node metastasis; right-sided pleural effusion; and a small amount of pericardial effusion and atelectasis of the middle and lower lobes of the right lung (Figure 1A).The patient was a former heavy smoker with a smoking index of 20 packs/year.Cytological examination of pleural fluid revealed the diagnosis of malignant pleural effusion with the tumor cells positive staining for NapsinA and thyroid transcription factor-1 (TTF-1; Figures 2A-C).The patient was diagnosed with a metastatic lung adenocarcinoma (cT4N2M1, stage IV).
The patient immediately received one cycle of pemetrexed disodium (500 mg/m 2 ) plus carboplatin (AUC 5) after diagnosis while waiting for the results of next-generation sequencing (NGS).Molecular analysis of pleural fluid by NGS based on a nine-gene panel was performed and showed the common EGFR exon 19 Radiographic evaluations of main lesions during the disease course.Frontiers in Immunology frontiersin.orgmutation (p.E746_A750del).The patient then received continuous oral icotinib (125 mg tid).After 1 month, CT showed a partial response (PR; Figure 1B).The clinical and morphological response was confirmed after 6 months since icotinib treatment.The patient continued icotinib beyond slow progression after 17 months of icotinib.The tumor continued to grow slowly over 3 months (Figure 1C), and a lung biopsy was obtained.The lung biopsy showed enteric differentiation components, which exceeded 50% (Figure 2D).Unfortunately, cell block at diagnosis was not available for further examination.Abdominal CT and colonoscopy examination revealed no evidence of gastrointestinal disease.Immunohistochemical (IHC) assays showed that the tumor was positive for TTF-1, NapsinA, CK7, and CDX2 (Figures 2D-H) and negative for programmed death ligand  region of MSH2, MSH6, MLH1, or PMS2 was identified.The patient received osimertinib (80 mg daily), which allowed an improvement for 3 months until reassessment CT revealed pulmonary progression (Figure 1D), accompanied with increased hemoptysis and cough.From November 2020 to April 2021, he received two cycles of nedaplatin (180 mg)-pemetrexed (900 mg)-bevacizumab (600 mg) therapy, then four cycles of combination therapy with nedaplatin (180 mg) plus pemetrexed (900 mg), and one cycle of pemetrexed (900 mg) monotherapy.Pulmonary CT scans showed PR after first cycle of nedaplatin-pemetrexed-bevacizumab treatment and disease progression in May 2021 [progression-free survival (PFS) 6.5 months, Figures 1E, F].Then, the patient received camrelizumab (anti-PD1, 200 mg) monotherapy (PFS 1 month) and camrelizumab (200 mg, q 3 weeks) plus pemetrexed (900 mg) therapy (Figure 1G).

Discussion
PEAC is a rare subtype of non-small-cell lung cancer (NSCLC), which was first reported by Tsao and Fraser as a case in 1991 (20).To date, over 200 cases have been reported (21).PEAC was officially defined as a rare variant of invasive lung adenocarcinoma by the WHO classification in 2015, with more than 50% of intestinal differentiation components, and the tumor cells should be positive for at least one immunohistochemical marker of enteric differentiation (1).The present case was first diagnosed as lung adenocarcinoma containing an EGFR mutation using tumor cells from pleural fluid.Then, the patient was diagnosed with adenocarcinoma with intestinal differentiation components from lung biopsy after resistance to first-line icotinib treatment.It may be difficult to diagnose PEAC using circulating tumor cells or a small sample of lung biopsy.Given the fact that PEAC is a mixed histological subtype of lung adenocarcinoma, the molecular differences between intestinal differentiation components and other non-intestinal differentiation components remain unknown.EGFR in-frame deletion of exon 19 (E19del) was reported in pulmonary enteric adenocarcinoma (1/10) (22).EGFR mutation may present in ordinary lung adenocarcinoma components, which decreased with the use of icotinib, while the intestinal differentiation components were insensitive to icotinib and increased during treatment.More molecular studies about the origin and intratumor heterogeneity of PEAC are needed in the future.
Owing to the rarity of disease, the molecular characteristics of PEAC have not been comprehensively determined.Several studies have described the genomic landscape of PEAC.High KRAS and MMR gene mutation rates and a low EGFR mutation rate were observed in PEAC (3,4).The present case had a common EGFR E19del mutation and had a PFS of 17 months on first-line icotinib treatment.Genomic sequencing of tumor biopsy after icotinib resistance revealed EGFR T790M mutation.The patient received a third-generation EGFR tyrosine kinase inhibitor (TKI), osimertinib, and achieved a PFS of 3 months.This study is the first to describe EGFR tyrosine kinase inhibitors in a PEAC patient with an EGFR-sensitive mutation.The firstand second-line target therapies indicated that a PEAC patient with an EGFR-activating mutation could also benefit from EGFR-TKI, and an NGS test after first-line TKI resistance could guide subsequent therapy.
At present, the treatment plan of PEAC has not been fully studied in previous literature.The present treatment strategy for advanced PEAC is the same as that of primary lung adenocarcinoma, including chemotherapy and radiotherapy and/or targeted therapy.Immunotherapy might be a useful treatment option for PEAC because of its high frequency of MMR mutation (21).According to IMpower150, the atezolizumab (anti-PD-L1) plus bevacizumab plus chemotherapy regimen showed a progressionfree and overall survival benefit when compared with the standardof-care bevacizumab plus chemotherapy regimen in EGFRm-TKI progressed patients (23).The ORIENT-31 trial consistently demonstrated that combination therapy of sintilimab plus chemotherapy with or without bevacizumab biosimilar IBI305 significantly improved PFS compared with chemotherapy alone in EGFRm-TKI progressed patients (24).To date, only two case studies have demonstrated checkpoint inhibitor therapy in PEAC and exhibited controversial results.A recently published case showed that a metastatic PEAC patient with a KRAS G12C mutation suffered hyperprogressive disease after one cycle of first-line paclitaxel plus carboplatin, along with sindilizumab (25).However, another recently published study demonstrated that primary and metastatic lesions were effectively treated by pembrolizumab plus carboplatin and pemetrexed in a PEAC with a KRAS G12D mutation (26).The discrepancy of clinical benefits between the two PEAC cases receiving first-line chemoimmunotherapy might be explained by the different NGS panels used and metastatic status, and the latter case received palliative radiation for bone metastases.The effectiveness of combination therapy with a checkpoint inhibitor and chemotherapy in PEAC remained uncertain.
Our patient only received a bevacizumab plus chemotherapy regimen, without combination with a checkpoint inhibitor after progressing on treatment with icotinib and osimertinib.Then, the patient changed to ICI monotherapy or chemoimmunotherapy after progressing on treatment with bevacizumab plus chemotherapy, but he benefited poorly from those treatments.The presence of an EGFR mutation and PTEN and JAK1 truncation mutations were negative predictors of immunotherapy (27)(28)(29) and might account for the treatment failure of the case.Accumulation of clinical experience in immunotherapy is necessary for better treatment of this rare lung cancer.Our case indicates poor benefit from immunotherapy for PEAC with an EGFR-sensitive mutation and MSI-H in later-line settings.
As we know, MSI-H is a more common molecular feature observed in colorectal and endometrial cancer compared with other solid tumors, while few studies concerned MSI status in lung cancer.Patients with MSI-H are more likely to benefit from immunotherapy across cancers (30).Polymerase chain reaction (PCR)-, IHC-, and NGS-based MSI analyses were commonly used in most clinical laboratories.However, it has been reported that approximately 5% of colorectal cancers that display retention of all four MMR proteins may indeed be MSI-H, possibly due to the heterogeneous expression of MMR proteins, or proteins emanating from abrogated MMR genes were still detected by IHC (31).The MLH1 c.332C>T mutation, a germline pathogenic mutation reported in colorectal cancers (32), was detected in the lung biopsy of our case with the highest VAF.Missense mutations of MMR genes in formalin-fixed paraffin-embedded (FFPE) tumor tissues were also detected in colorectal cancer cases with pMMR and MSI-H (31).In lung cancer, 0.17% (2/1,153) and 0.5% (66/12,484) patients were reported to be MSI-H via IHC and the NGS-based method, respectively (33,34).Recent studies showed all tumor tissue samples were microsatellite stable (MSS) in PEAC according to PCR-(17 cases) or IHC-based (8 cases) MSI analysis.The present case was a rare lung adenocarcinoma with enteric differentiation, pMMR, and MSI-H.Further studies in cases with somatic MMR gene mutations and MSI-H may help elucidate the phenomena.
In summary, this is the first case to describe an EGFR-mutated lung adenocarcinoma that had enteric differentiation components, EGFR T790M, and MSI-H after resistance to first-line icotinib and responded poorly to osimertinib and immunotherapy.Few reports to date have described the sequential treatment of PEAC with EGFR-TKIs and immunotherapy.The findings observed in this patient, including diagnoses, treatments, and the association between clinical outcomes and driver genes, may lead to future studies on the origin, diagnosis, and treatment of patients with PEAC.
(A) Computed tomography (CT) scan at diagnosis.(B) CT scan showed lesions shrank after 1 month of first-line icotinib; (C) CT scan after resistance to icotinib.(D) CT scan after 3 months of osimertinib treatment; (E) CT scan before the second cycle of nedaplatin-pemetrexed-bevacizumab showing partial response; (F) CT after 6 months of third-line therapy showing disease progression; (G) CT scan after 2 months of immunotherapy.RUL, right upper lobe; RLL, right lower lobe; RML, right middle lobe; PR, partial response.Yang et al. 10.3389/fimmu.2023.1266304