Polysaccharide extracted from pomelo fruitlets attenuate hepatic lipid accumulation in hybrid groupers (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀)

Introduction Pomelo is one of the most consumed fruits due to its distinct flavor and sour taste, while large quantities of pomelo fruitlets discard during cultivation and most of them are disposed of as wastes. Such an issue has led to some research on how to make a high value-added reutilization of these fruitlets. Therefore, it was aimed to determine the structural characteristics of the polysaccharide of pomelo fruitlets and evaluate its attenuating effect on the hepatic lipid accumulation in hybrid groupers (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀). Methods and results In this research, YZ-0.5A is a 15,332 Da NaCl-soluble polysaccharide extracted from pomelo fruitlet which was characterized to mainly contain galactose and galacturonic acid and its putative structure was proven to be →2,4)- α-L-Rhap-(1→4)-α-D-GalAp-(1→4)-α-D-GalAp-(1→ with 3 branches. In vivo study, five isolipidic diets (containing 15% lipid) were fed to the groupers with an initial weight of 13.46 ± 0.08 g for 8 weeks, of which the dietary level of YZ-0.5A was 0 mg/kg (control), 150 mg/kg, 300 mg/kg, 600 mg/kg and 1200 mg/kg respectively. We found that the treatment of YZ-0.5A, especially 600 mg/kg, exerted an improved effect on the excessive lipid accumulation in grouper fed with a high-fat diet. In vitro, three graded concentrations of YZ-0.5A (75, 150 or 300 μg/ml) were added to the grouper primary hepatocytes respectively after incubation with 20% lipid emulsion (2 ml/L). The signs of recovery on morphological features observed under examined by histological evaluation supported the lipid lowering effect of YZ0.5-A. Further investigation showed that YZ0.5-A mitigated lipid emulsion-induced irregular lipid deposition by regulating various lipometabolism-related indicators, thereby alleviating oxidative stress and apoptosis. Discussion Overall results exhibited the therapeutic potential of YZ0.5-A and elucidated its underlying mechanism in the fish with excessive lipid deposition, which first suggested the feasibility of reprocessing discarded pomelo fruitlet as medicine in aquaculture.


Introduction
Excessive fat is currently served as a partial protein substitute to optimize aquafeed costs in intensive aquaculture . Although a certain range of dietary lipids reportedly has an improved effect on a fish's growth performance (Zou et al., 2019), excessive lipid intake can inversely result in unwanted lipid deposition in fish (particularly in the liver) and augment vulnerability to an irregular metabolism, as previously reported in grass carp (Ctenopharyngodon idella) (Jia et al., 2020)and tilapia (Oreochromis niloticus) . High lipid accumulation in the liver is considered to be the leading contributor to fatty liver disease in farmed fish, which induces several metabolic disorders, including oxidative stress, inflammation, lipotoxicity, and apoptosis . The occurrence of fatty liver disease in fish is everincreasing year by year, seriously threatening the sustainable development of the aquaculture industry.
Polysaccharides, due to their manifold pharmacological functions and reduced side effects, have been increasingly adopted in many fields, including the food, chemical, and pharmaceutical industries (Benhura and Chidewe, 2002). Recently, a growing body of literature has shown the pharmacological effect of polysaccharides, particularly extracted from plants, in diseases inflicted by abnormal lipid metabolism, such as fatty liver disease  and diabetes (Khursheed et al., 2020), which suggests its potential fat lowering effect. The polysaccharides added to the aquafeed are generally used as feed additives or functional supplements to promote growth performance, enhance immunity response, and ameliorate antioxidant status Yu et al., 2022). However, the discussion over whether polysaccharides have a positive effect on fish suffering from diet-induced lipid metabolic disorders is still ongoing.
Pomelo (Citrus grandis Osbeck) is a citrus fruit grown in South China that produces a mass of discarded and unmarketable fruitlets because of physiological fruit drop or artificial fruit thinning during cultivation. These fruitlets discarded in the orchard not only cause considerable financial losses to farmers but also result in environmental pollution due to their easy fermentability. Hence, inventing new and profitable ways to reuse these fruitlets is necessary and meaningful. Noticeably, the pomelo's counterparts in the citrus class have shown a positive effect on lipid metabolism. For example, dietary hesperetin improves hepatic triglyceride accumulation in rats fed orotic acid (Cha et al., 2001). Moreover, Zang et al. (2014) found that the peel of Yuzu (Citrus junos Siebold ex Tanaka) ameliorates diet-induced obesity in zebrafish (Danio rerio). Considering the reported bioactive effects on lipid metabolism of polysaccharides and citrus fruit, we hypothesized that the polysaccharide derivative from pomelo fruitlets may be reutilized as an aquafeed additive to normalize the diet-induced lipid metabolic disturbance.
The hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀) has been largely farmed in southeastern coastal areas of China with the advantages of fast growth rate, strong resistance, and high economic and nutritious value (Liang et al., 2020). In the present study, we investigated the structure of the polysaccharide YZ0.5-A extracted from pomelo fruitlets and evaluated the effects of YZ0.5-A on lipid accumulation in the hybrid groupers in vivo and in vitro.

Sinopharm Chemical Reagent (Shanghai, China). DEAE-Sepharose
Fast Flow and Sephadex G-200 were purchased from Sigma-Aldrich (St. Louis, MO, USA). MEM culture medium was purchased from Thermo Fisher Scientific (SUZHOU) Instruments Co., Ltd. phosphate buffered saline (PBS) was purchased from Yongjin Biotechnology Co., Ltd. (Guangzhou, China). CCK-8 assay kits were purchased from the Biyuntian Institute of Biotechnology (Shanghai, China). The ELISA Kits for the determination of Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione reductase (GR) were purchased from the Beyotime Institute of Biotech (Shanghai, China). lipid emulsion (LE) was purchased from Guangzhou Panyu Armed Police Hospital (Guangzhou, China). All other reagents and chemicals used in this study were of analytical grade.
The juvenile hybrid groupers (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀) used in the experiments were purchased from the Daya Bay Breeding Test Center in Huizhou City, Guangdong Province, China.

Extraction and purification
Briefly, the pomelo fruitlets were washed, chopped in granular form, and powered by the grinder. Then, the powders were extracted using pectinase and cellulase (2% w/v, pH 6.0, 50°C, 2 h) with distilled water at a ratio of 1:30 (w/v). After enzyme inactivation and sonication (Wu et al., 2020), the reaction mixture was centrifuged (3622 × g) and the supernatant liquor was concentrated. Subsequently, the collected solution was deproteinized using 10% trichloroacetic acid , and precipitated with ethanol. Then, the samples were dissolved in deionized water and lyophilized to give the crude polysaccharide, which was next separated using a DEAE-Sepharose Fast Flow chromatography column (2.5 × 60 cm), eluted by ultrapure water and NaCl solution (0.2, 0.5, or 2 M, pH 7.0), and then purified using a Sephadex G-100 column (1.6 cm × 60 cm) .

Structural characterization 2.3.1 Fourier transform infrared spectroscopy
Samples (2 mg) were mixed with 200 mg KBr, ground, and then pressed into pellets for FT-IR scanning. Spectra were collected in the frequency range of 4000-400 cm −1 .

Molecular weight determination
The molecular weight distribution of YZ-0.5A was analyzed by high performance gel-permeation chromatography (HPGPC) using a Shimadzu LC-10A system linked to a Shimadzu RI-502 .

In vitro studies
Primary hepatocytes of the juvenile hybrid grouper were isolated and cultured according to the method of (Zou et al., 2019). The primary hepatocytes were cultured in MEM containing 1% (v/v) penicillin-streptomycin double antibody (negative control) and 10% (v/v) FBS at 25°C with 5% CO 2 . After culturing the hepatocytes for 24, 48, and 72 hours, the YZ0.5-A dosage concentrations were selected for follow-up experiments according to cell viability. The groups and the treatment method were as follows. Control group: hepatocytes alone; model group (LE for 72 h); and recovery group: hepatocytes were treated with LE for 48 h, and then incubated with normal medium for 24 h; YZ0.5-A group: hepatocytes were incubated with LE for 48 h, and then incubated for 24 h with 75, 150, or 300 mg/mL YZ0.5-A). Samples were analyzed after 72 h.

Experimental procedures
The experimental procedures were similar to those described in our previous study (Zou et al., 2019). Three hundred juvenile groupers (13.46 ± 0.08 g) were randomly divided into five experimental groups, with three replicates in each group (15 floating cages). Fish were fed to apparent satiation two times daily at 8:00 and 16:00. The feeding trial lasted for 8 weeks, during which the number of dead fish, the weight of dead fish, and feed consumption were recorded daily. At the end of the feeding trial, fish were fasted for 24 h and euthanized with 100 mg/L MS222 to detect the weight gain (WG) and condition factor (K). Blood samples were collected by caudal venipuncture using 2 ml syringes to analyze biochemical indices. After blood withdrawal, the fish were dissected for the calculation of hepatosomatic index (HSI), and viscerosomatic index (VSI). Then, liver samples were removed and stored at −20°C for further analyses. All experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of Zhongkai University of Agriculture and Engineering (ethics code permit number: ZK20180518).
2.6 Sample analysis 2.6.1 Cell viability assay The viability of hepatocytes treated with YZ0.5-A was evaluated using cell counting kit-8 assays (CCK-8; ABP Biosciences, Beltsville, MD, USA) according to the method used in our previous study .

Histopathological analysis
Hematoxylin and eosin (H&E) staining on the liver tissue and hepatocytes of hybrid grouper was conducted according to previous methods (Zou et al., 2022). In vivo experiment, dissected liver specimens were fixed at 4% neutral buffered formalin and dehydrated in graded ethanol concentrations and impregnated with paraffin. Subsequently, the paraffin-soaked specimens were frozen and cut into 5-mm thickness sections in the cryostat. The sections were stained with hematoxylin and eosin (H&E) for optical examination. In vitro experiment, isolated hepatocytes were washed with phosphate buffered saline (PBS), fixed with 4% paraformaldehyde for 20 min, and rinsed in 0.1M PBS×4 for 2 min each time. Then staining with H&E was performed after the removal of PBS.A Light microscope (200×, MshOt MS60) was used to picture the morphological features of the processed samples.

Histochemical analyses
Liver tissues were embedded in 4% buffered formaldehyde and processed into frozen thickness sections (9-mm) as described in 2.6.2. The sections were then stained with Oil Red O solution. The hepatocytes were fixed as described in 2.6.2, immersed in 60% isopropanol for 3-5s and rinsed in distilled water. The cells were stained for 8-10 min with filtered oil red O in dark, then exhaustively rinsed with water. The nuclei were counterstained with hematoxylin for 3-5 min. After one further rinse in water, intracellular lipid droplets were observed using microscope (MshOt MS60). The relative area of vacuolation and lipid droplets in stained samples was analyzed by Image-Pro Plus 6.0. stained samples.

Chemical analysis
Whole body and muscle moisture were determined using the Kjeldahl method. Crude protein (N×6.25) was measured after acid digestion using a Kjeltec 8400 (FOSS, Hilleroed, Denmark). Crude lipid was determined using ether extraction by Soxtec ™ 2055 (FOSS, Hoganos, Sweden). The samples were dried in the oven at 150°C for 24 h to measure moisture content. Ash in the whole body, muscle, and diet was measured with a muffle furnace (FO610C; Yamato Scientific Co., Ltd., Tokyo, Japan) at 550°C for 24 h.

Biochemical assays
Biochemical indicators of antioxidation, including CAT, SOD, GR, and GSH-Px activity were evaluated using detection kits (Jiancheng Institute of Biotechnology, Nanjing, China) in a spectrophotometer following the method previously described .

Cell apoptosis analysis
Apoptotic cells were evaluated by flow cytometric analysis after using Annexin H-APC/7AAD detection kit (Multi sciences, Shanghai, China) following the manufacturer's instructions. Briefly, hepatocytes (1 × 10 6 cells) were reseeded in 6-cm dishes and proceeded with Annexin V-APC/7-AAD double staining, fixed at 28°C for 15 min, and subsequently stored in ice. Then, cells were analyzed by flow cytometry.

Real-time qPCR
Total RNA was isolated from the liver samples using TRIzol reagent (Invitrogen, Waltham, MA, USA). mRNA quality was measured by spectrophotometry (A260:280 ratio) and electrophoresis using 1.2% agarose gels. Then, cDNA was synthesized using an Easy Script First-Strand cDNA Synthesis SuperMix (Transgen Biotech, Beijing, China). The specific primers for each gene are listed in Table 2 Zou et al., 2021). The real-time PCR reactions were carried out in a final volume of 20 ml, using 1×Power SYBR Green PCR MasterMix buffer (ABI, USA) on a Step-one PCR amplifier (ABI, USA). Cycling parameters were as follows: 1 min at 94°C, followed by 40 cycles of 10 s at 94°C; 40 cycles of 20 s at 60°C; and 40 cycles of 30 s at 72°C. All assays were carried out in triplicate. The mRNA expression levels were normalized to the geometric mean of the reference genes (b-Actin) using the 2 −DDCt method.

Statistical analysis
Data were expressed as mean ± standard deviation of three biological replicates. One way analysis of variance was used to determine significant differences at P< 0.05. Linear and quadratic effects of YZ-0.5A supplementation at increasing levels in vivo were evaluated using an orthogonal polynomial contrast test. All statistical analyses were conducted using SPSS 11.0 software (SPSS Inc., Chicago, IL, USA).

Results and discussion
3.1 Structure analysis

Extraction and purification
The NaCl-soluble polysaccharide YZ-0.5A was extracted from pomelo fruitlets and obtained after deproteinization. The isolated YZ-0.5 was first fractionated with a DEAE-Sepharose Fast Flow chromatography column and eluted with 0.5 M NaCl solution. After Sephadex G-200 gel chromatography, the curve showed that a YZ-0.5A fraction was obtained from YZ-0.5 ( Figure 1A). YZ-0.5A is an acidic polysaccharide, as it was eluted with NaCl solution. When the NaCl concentration was increased to 0.5 M, the production of soluble sugar concentration increased to a maximum and then decreased with increasing NaCl.

FT-IR of YZ-0.5A
The FT-IR spectrum of YZ0.5-A is shown in Figure 1B. The broad peak at approximately 3600-3200 cm −1 was due to the stretch vibration of the -OH bond, which was the characteristic polysaccharide peak. The band at 2935 cm −1 was the characteristic C-H absorption peak. The absorption peak at 1610 cm −1 represented N-H stretching vibration (PoMohammadur Dounighi et al., 2015). The absorption bands at 1427, 1338, 1240, and 1016 cm −1 represented C-O, C=O, and O-H vibrations (PoMohammadur Dounighi et al., 2015;Amutha et al., 2017). Further, the characteristic absorption band at 890 cm −1 was assigned to the pyran structures, which might be caused by C-H stretching vibration. YZ0.5-A may be a b-terminus differential isomer, but the b-terminus configuration needed to be further determined by nuclear hydrogen and carbon spectra. According to our previous research, the crude polysaccharide extracted from pomelo fruitlets is a residue polymer containing acidic monosaccharides with pyran rings, which is consistent with this result.

Molecular weight
According to the HPGPC analysis, there was only one symmetrical signal peak in the molecular weight profile of the YZ0.5-A fractions ( Figure 1C), indicating high sample purity. The estimated average molecular weight of the YZ0.5-A fractions, based on calibration with the standard, was calculated to be 15,332 Da. Previous studies have suggested that biological activity is closely related to molecular weight (Sheng and Sun, 2014). If the molecular weight of the polysaccharide is too small or too large, the molecule usually has no biological activity (Huang et al., 2020). Thus, proper molecular weight distribution can denote good biological activity (Zhang et al., 2016).

Monosaccharide composition
The anion chromatography analysis of 12 standard monosaccharides is shown in Figure 1D and the chromatogram ( Figure 1E) revealed five peaks indicating that YZ0.5-A was heterogeneous. Based on the retention time of each monosaccharide, the monosaccharide composition in YZ0.5-A included Rha, Ara, Gal, Glu, and GalA in a molar ratio of 0.088: 0.157: 0.331: 0.123: 0.301. As the previous study regarding the structure-activity relationship of polysaccharide reported, polysaccharides with an MW range of 4000-100,000 Da and a high proportion of GalA and Ara could show appreciable bioactive, like antioxidant activity (Li et al., 2016). Hence, YZ0.5-A with 15,332 Da and containing nearly 45% of GalA and Ara could be postulated to have bioactive potential.

NMR spectroscopy analysis
The 1D NMR spectrum of YZ0.5-A, including 1 H, 13 C, and Dept135, is shown in Figures 2A-C. The proton spectrum signals    The anomeric hydrogen signal of residue D was designated as d4.42 ppm, and its signals of H2-H6a could be obtained from the COSY spectrum ( Figure 2E), which are d4.42, 3.57, 3.68, 4.05, 3.87, and 3.96. Meanwhile, as a reference to the HSQC spectrum ( Figure 2D), the corresponding carbon signals of H2-H6a could be inferred to be: 72.23, 81.54, 70.60, 75.25 and 70.60. Therefore, based on these results and the literature, residue D was considered as !3,6)-Galp-(1! (Hu et al., 2015). In this way, the hydrogen and carbon signals of all other residues could also be obtained and inferred to the possible glycosidic bond. Hence, all glycosidic bonds were determined and assigned, as shown in Table 4.
Some legible cross-peaks between the 1 H and 13 C signals of different sugar residues could be observed in the HMBC spectrum ( Figure 2F), which are: h H1 and j C2, j H1 and j H4, e C1 and e H4, e C1 and h H4, b C1 and l H6, f C1 and d H3 and d C1 and h H4. These cross-peaks corroborated the presence of !2,4)-a-L-Rhap-

In vivo study 3.2.1 Whole body and muscle composition
The effect of dietary YZ0.5-A on the composition of the whole body and muscles of hybrid groupers is shown in Table 5. Dietary YZ0.5-A significantly decreased the crude lipid content throughout the whole body (P< 0.05). Dietary YZ0.5-A had a significant effect on crude protein and crude lipid levels in the muscles of hybrid groupers (P< 0.05). The addition of 300-600 mg/kg YZ0.5-A to feed significantly reduced the overall and muscle lipid content. These results indicate that supplementing YZ0.5-A in feed reduces lipid deposition in fish.

Growth performance, feed utilization, morphological parameters and histochemical examination
Previous studies showed that the appropriate lipid content of the juvenile hybrid grouper feed is 7%-13% (Jiang et al., 2016). When the fat content in the diet of hybrid grouper reaches 15%, there is significant fat deposition in the liver or visceral fat tissue in farmed fish (Jia et al., 2017;Zhang et al., 2019a). The effects of YZ0.5-A on the growth performance, feed utilization, and morphological parameters of hybrid groupers fed by a high-fat diet (HFD) are shown in Table 6. The WG of hybrid groupers in the YZ-0.5-600 groups was significantly higher than those in the YZ-0.5-0 group (P< 0.05). Except for that in the YZ-0.5-1200 group, the FE of the YZ-0.5-0 group was lower than that of the other experimental groups (P< 0.05). HSI, VSI, and K were all decreased, albeit with varying degrees, in the YZ0.5-A treated groups (particularly in YZ-0.5-600 group) compared with the control group (P< 0.05). The decreases of VSI and HSI might be due to the loss of lipid in the liver, which was confirmed by the performance of the Oil Red O-stained liver sections ( Figure 3B). The lipid droplet clusters in the YZ-0.5-300, -600, and -1200 groups was reduced relative to the YZ-0.5-0 group. Collectively, these results suggest that YZ0.5-A has a positive effect on growth performance and prevents lipid accumulation and liver enlargement in fish.

Expression of lipid accumulation-related genes in vivo
We further assessed the lipid-lowering effect of YZ0.5-A in HFDfed hybrid grouper using RT-qPCR. As shown in Figure 3C, the expression of diacylglycerol O-acyltransferase 2 (DGAT2), glucose-6phosphate dehydrogenase (G6PD), malic enzyme 1 (ME1), and fatty acid synthase (FAS), genes related to lipid synthesis (Zou et al., 2019), exhibited no difference in the YZ-0.5-150 to -300 groups but  Values are means ± SD (n=6) of three replicates. Means in the same row with different superscripts indicate significant differences (P< 0.05). Orthogonal polynomials were used to evaluate linear and quadratic responses to the levels of YZ-0.5A treatments. IBW, initial body weight; FBW, final body weight; WG, weight gain; SGR, specific growth rath; FE, feed efficiency; HSI, hepatosomatic index; VSI, viscerosomatic index; K, condition factor. significantly declined in the YZ-0.5-600 group compared with that in the control (P< 0.05). Contrarily, the expression of the above factors was reversely elevated in the fish supplemented with 1200 mg/kg YZ0.5-A (P< 0.05), inferring that a specific concentration range of YZ0.5-A could inhibit lipid generation, while a high dose treatment may result in an adverse effect. In contrast, the mRNA expression of the lipolysis-related genes: lipoprotein esterase, Aconitase 1 (ACO1), peroxisome proliferator-activated receptor alpha (PPARa), and carnitine palmitoyltransferase 1 (CPT1) (Tan et al., 2019) was also investigated, and the expression was mostly enhanced after the addition of YZ0.5-A (P< 0.05). These results imply that treatment with YZ0.5-A improves lipid utilization in fish under HFD feeding.

In vitro study 3.3.1 Cell viability
To determine the optimal YZ0.5-A treatment time and concentration for hepatocytes and to investigate the YZ0.5-A concentration that mitigates hepatocyte viability after treatment with 2 mL/L 20% LE, we incubated hepatocytes with different YZ0.5-A concentrations for 24, 48, and 72 h. As shown in Figure 4A, all YZ0.5-A concentrations (except 150 and 300 mg/ mL) decreased hepatocyte survival after 24 h. However, with extended culture time, the effect was changed. Cell viability was stable in the YZ0.5-A groups by 48h. While the obvious reduction of viability was observed in cells treated YZ0.5-A with concentration beyond 300 mg/mL compared with that in the control group by 72 h (P<0.05). Thus, we chose these three concentrations as the low-, medium-, and high-dose groups, respectively, for 24-h cell incubation. Compared with that in the control group, the results showed that hepatocyte viability of the model group significantly decreased (P< 0.05), while hepatocyte viability in the recovery group was significantly increased compared to that in the model group (P< 0.05). Viability was also remarkably decreased compared to that in the YZ-0.5-150 group (P< 0.05). Compared with that in the model group, the survival rate of the recovery group was increased, but compared to that in the YZ0.5-150 group, the survival rate was significantly decreased (P< 0.05). Therefore, 75, 150, and 300 mg/mL YZ0.5-A was used for subsequent tests.

Histological and histochemical examination of hepatocytes
H&E staining was used to analyze the hepatocyte structure ( Figure 4C). In the control group, the hepatocyte nuclei were normal and neatly arranged. In the model group, the cell structure was destroyed, and vacuoles formed. In the recovery group, the cell state recovered slightly, and the structural damage improved. YZ0.5-A addition reduced the amount of hepatic cytoplasmic vacuolation, and the disrupted cell morphology was alleviated. Further, YZ0.5-A reduced the number of lipid droplets in hepatocytes. The Oil Red Ostained area in the YZ0.5-150 group were smaller than that in other groups ( Figure 4D). Moreover, the morphological changes shown with H&E and Oil Red O staining were similar to those in the in vivo experiments ( Figures 3A, B), giving further support to the therapeutic function of YZ0.5-A in morphological lesions inflicted by excessive lipid administration. Additionally, these results were in agreement with our previous hybrid grouper study that investigated another plant extract, Radix Bupleuri (Zou et al., 2019).

YZ0.5-A alleviates the hepatic steatosis caused by LE via the AMP-activated protein kinase pathway
Lipids play a key role in growth and development. In fish, excessive lipid deposition in the body, especially in the liver, reduces growth rate and causes serious health problems, which poses a serious threat to the sustainable development of aquaculture . In the present study, as exhibited in Figure 4F, YZ0.5-A treatment significantly offset the LE-induced increase in the mRNA expression of sterol regulatory element-binding protein-1c (SREBP-1c), a transcriptional factor that is conducive to synthesizing lipids, and its functional downstream target, FAS (Ma et al., 2020). Moreover, the transcriptional levels of other genes related to lipid synthesis (G6PD and ME1) were also markedly suppressed in the YZ0.5-A treatment groups relative to the model and recovery group (P<0.05). PPARa, an important transcriptional factor which could reportedly upregulate the gene expression of the lipid oxidationrelated enzyme CPT1, thus promoting lipolysis (Tao et al., 2019). Compared with those in the non-YZ0.5-A treated group, the expressions of PPARa and CPT1 were markedly enhanced in the YZ0.5-A treated group (P< 0.05). These findings indicated that YZ0.5-A shows its therapeutic effect against hepatic steatosis by intensifying fatty acid synthesis while promoting fatty acid utilization. The above performance with H&E and oil red O staining morphologically confirmed this conclusion.
AMPK, a heterotrimer comprised of an a-, b-, and g-subunits, is a well-documented target of many therapeutic regimens for fatty liver disease (Smith et al., 2016). Specifically, AMPK activation plays a critical role in regulating lipid metabolism by suppressing SERBP-1c (Sharma et al., 2020). It has been also reported that activation of AMPK could trigger PPARa and thus improve fatty acid oxidation (Cheng et al., 2015). In this study, the LE-induced reductions in the mRNA expression of AMPK-a, -b, and -g in the model group were significantly replenished in the YZ0.5-A-treated groups, particularly at 75 and 150 μg/mL ( Figure 4F; P< 0.05). Considering the gene expression changes noted in the previous paragraph, these results suggest that YZ0.5-A may influence the activity of AMPK and secondarily its downstream target lipid metabolism-related genes, hence mitigating LE-induced hepatic steatosis.

YZ0.5-A ameliorates oxidative stress and apoptosis inflicted by irregular lipid metabolism
Recent research has shown that lipid accumulation propels the onset of oxidative stress and thus impairs the antioxidant system, which is mainly manifested as a suppressed indicator of antioxidative enzymes (Hong and Lee, 2009). Accordingly, our experiment exhibited a similar effect, as LE challenges cause a reduction in the enzymatic activities of CAT, SOD, GR, and GSH-Px ( Figure 4B; P< 0.05). Furthermore, apoptosis is regarded as a mechanism involved in the pathophysiologic progress of liver injury induced by excessive lipid deposition, which has occurred in various hepatocyte models, including mice (Xiao et al., 2013), chicken (Ma et al., 2020) and fish (Zhang et al., 2019b). In line with previous investigations, an obvious elevation in the apoptotic rate detected by flow cytometry could be observed in the model and recovery groups relative to that in the control ( Figure 4E; P< 0.05). Noticeably, the aforesaid pathological performance was significantly reserved in the subsequent experiment with the supplementation of YZ0.5-A (especially at 150 mg/mL), indicating its protective effect on the onset of oxidative stress and apoptosis induced by irregular lipid metabolism.

Conclusion
In this study, the polysaccharide YZ0.5-A was isolated from pomelo fruitlets. The yield was 9.5%. Based on the monosaccharide, methylation, FT-IR, and NMR spectrum analysis results, YZ0.5-A was identified as a 15332 Da heteropolysaccharide which is mainly composed of 1,5-linked Araf (22.40%), 1,4-linked Glcp (14.42%), and 1,4-linked Galp (17.68%). The main chain is speculated to be !2,4)-a-L-Rhap-(1!4)-a-D-GalAp-(1!4)-a-D-GalAp-(1!. The results of the in vivo study demonstrated for the first time in fish that dietary YZ0.5-A has a positive effect on growth performance and fat loss. Additionally, the in vitro experiments indicated that YZ0.5-A may alleviate unwanted lipid accumulation in fish liver, and further inhibit oxidative stress and cell apoptosis. Collectively, the study findings indicated that YZ0.5-A appreciably offset the undesirable outcomes originating from HFD feeding, which not only increases the acceptability of feeding HFD to farmed fish but also provides a new prospective for the reutilization of wasted pomelo fruitlets.

Data availability statement
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Ethics statement
All experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of Zhongkai University of Agriculture and Engineering (ethics code permit number: ZK20180518). Written informed consent was obtained from the owners for the participation of their animals in this study.

Author contributions
CZ, HL, and GX contributed to the conception and design of the study. CZ and HL took samples of experimental animals. CZ, HL, and YF performed and analyzed all the other experiments. YF and NL wrote the manuscript with support from all authors. LL contributed to the revising of the manuscript. All authors contributed to the article and approved the submitted version.

Funding
This work was supported by the Guangdong Provincial Natural Science Foundation Project [grant number 2019A1515011283].