AUTHOR=Orfanakis Georgios , Patila Michaela , Catzikonstantinou Alexandra V. , Lyra Kyriaki-Marina , Kouloumpis Antonios , Spyrou Konstantinos , Katapodis Petros , Paipetis Alkiviadis , Rudolf Petra , Gournis Dimitrios , Stamatis Haralambos 

TITLE=Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties

JOURNAL=Frontiers in Materials

VOLUME=5

YEAR=2018

URL=https://www.frontiersin.org/journals/materials/articles/10.3389/fmats.2018.00025

DOI=10.3389/fmats.2018.00025

ISSN=2296-8016

ABSTRACT=<p>Hybrid nanostructures of magnetic iron nanoparticles and graphene oxide were synthesized and used as nanosupports for the covalent immobilization of β-glucosidase. This study revealed that the immobilization efficiency depends on the structure and the surface chemistry of nanostructures employed. The hybrid nanostructure-based biocatalysts formed exhibited a two to four-fold higher thermostability as compared to the free enzyme, as well as an enhanced performance at higher temperatures (up to 70°C) and in a wider pH range. Moreover, these biocatalysts retained a significant part of their bioactivity (up to 40%) after 12 repeated reaction cycles.</p>