%A Kakimoto,Yasuhiro
%A Tero,Ryugo
%D 2018
%J Frontiers in Materials
%C
%F
%G English
%K Supported lipid bilayer,E coli lipid,Lipid organization,Atomic Force Microscopy,fluorescence microscopy,Fluorescence Recovery After Photobleaching
%Q
%R 10.3389/fmats.2018.00048
%W
%L
%M
%P
%7
%8 2018-August-21
%9 Original Research
%#
%! E. coli lipid bilayers and Ca2+ effects
%*
%<
%T Supported Lipid Bilayers of Escherichia coli Extracted Lipids and Their Calcium Dependence
%U https://www.frontiersin.org/articles/10.3389/fmats.2018.00048
%V 5
%0 JOURNAL ARTICLE
%@ 2296-8016
%X Formation of supported lipid bilayer (SLB) and additional structures of Escherichia coli (E. coli) lipids were investigated with fluorescence microscopy and atomic force microscopy. Ca2+ in the aqueous phase with concentration above 2 mM was necessary for the formation of SLB. Additional lipid structures, string-like structures, the second lipid bilayer, and multilayer stacking appeared on the first layer SLB depending on Ca2+ concentration. The bridging effect of Ca2+ between the negatively charged E. coli lipid bilayers and substrate is the dominant factor determining the two-dimensional and three-dimensional morphology of the E. coli lipid bilayer membranes.