Hot Tensile Deformation Behavior of Mg-4Li-1Al-0.5Y Alloy

The microstructure evolution and deformation mechanism of the as-extruded-annealed Mg-4Li-1Al-0.5Y alloy (denoted as LAY410) were investigated during the hot tensile deformation at the temperatures between 150°C and 300°C with strains from 8 × 10−5 s−1 to 1.6 × 10−3 s−1. The results show that when the strain rate decreases and/or the deformation temperature increases, the peak stress of the alloy gradually decreases, and the elongations to fracture gradually increases. The true stress–strain curves show typical dynamic recrystallization (DRX) softening characteristics. It is observed that the microstructure in the magnesium (Mg) alloy deformed at 150°C is mainly composed of the deformed grains and a few recrystallized grains. The microstructures in the Mg alloy deformed at 200°C consisted of substructures and a slightly increasing number of dynamic recrystallized grains. When the deformation temperature reaches 250°C, the number of recrystallized grains increases significantly, and the microstructures are dominated by recrystallized grains. Moreover, through theoretical calculation and result analysis, the activation energy was about 99.3 kJ/mol, and the hot tensile deformation mechanism was the alternate coordinated deformation mechanism among grain boundary slip (GBS), intragranular slip, and DRX.


INTRODUCTION
Compared with steel and aluminum (Al) alloy, Mg alloy not only has lower density but also good thermal conductivity and machinability. It has great application potential in aerospace, automobile manufacturing, and electronics industry (Luo et al., 1995;Wang, 2007;Hadadzadeh and Wells, 2013). However, Mg alloy is an HCP structure, and its slipping systems are less than five independent slip systems at lower temperatures (lower than 225°C). Therefore, it does not meet the requirements of the five slip systems required for continuous coordinated deformation of materials (He et al., 2019), which severely limits the wider application of Mg alloys. When the plastic forming temperatures are higher than 225°C, the <a> slipping system of the prismatic plan and <c+a> slipping system of the pyramidal plan II can be activated, although the plastic formability of the Mg alloys are improved at this situation, the costs have risen.
To improve the plastic formability of Mg alloys at room temperature, the alloying elements such as lithium (Li), Al, zinc (Zn), and rare earth element (RE) are usually added to Mg alloys to improve its plastic formability. The literatures (Al-Samman, 2009;Tang et al., 2017;Tu et al., 2020;Wang et al., 2020) found that adding Li to Mg alloys can effectively reduce the density and lattice spacing (c/a axis ratio) of Mg alloys. This makes the non-basal slip system of the alloy easier to be activated during deformation, thereby effectively improving the plastic formability of the alloy. In addition, Kumar et al. (2012) found that adding Li to Mg-Al alloy can promote the precipitation process of the MgAlLi 2 phase, which strengthens the alloy by the precipitation phase and thus improves the mechanical properties of the alloy. However, MgAlLi 2 is not thermally stable and tends to be decomposed at 50-70°C, and is further decomposed to form the AlLi phase, which leads to a significant decrease in the mechanical properties of the Mg-Li-Al alloy at elevated temperatures. This phenomenon prevents Mg-Li-Al alloys from having ultra-low density and high strength simultaneously (Cui et al., 2011;Hagihara et al., 2019;Li et al., 2019). Therefore, it is important to improve the comprehensive mechanical properties of Mg-Li-Al alloy. The previous studies (Hantzsche et al., 2010;Bao et al., 2013;Zhu et al., 2014;Fei et al., 2015) have shown that the addition of rare earth element yttrium (Y) can effectively improve the comprehensive mechanical properties of Mg-Li-Al alloys. Guo et al. (2014) studied Mg-9Li-6Al-xY (x 0, 2) and found that adding rare earth element Y could not only precipitate Al 2 Y phase in the alloy to suppress the precipitation of AlLi phase, but effectively refine the grains as well, thus improving the comprehensive mechanical properties of the alloy. In addition, Sun et al. (2018) found that the addition of rare earth element Y can increase the bonding force between atoms, thus effectively inhibiting dislocation slip during deformation. Therefore, the work hardening rate in the hot deformation is increased, and the mechanical properties of the alloy at elevated temperatures are significantly improved.
Recently, many researchers have studied the hot deformation behavior and microstructure evolution of Mg-Li-Al alloy in order to improve the mechanical properties and plastic formability of the alloy (Yang et al., 2016;Chen D et al., 2020;Guo et al., 2020). Xu et al. (2015) studied the hot deformation behavior of Mg-Li-Al-Nd alloy at varied temperatures and strain rates. It is found that unlike the LAY410 alloy, in this alloy, Li exists in the form of β-Li phase. The DRX easily occurs in the β-Li phase, whereas in the α-Mg phase, it is retarded. Moreover, the deformation temperatures and strain rates have great influence on the occurrence of DRX, and DRX can easily occur at higher temperatures and lower strain rates. Therefore, for the Mg-Li-Al-Nd alloy, the improvement of plastic formability of the alloy can be attributed to DRX softening and non-basal slip system activation during the hot deformation. Li et al. (2021) studied the hot tensile deformation behavior of LAZ532 alloy. It is found that the hot tensile deformation mechanism of the alloy is more complicated, which is an alternate coordinated deformation mechanism between GBS, intragranular slip, and DRX. However, there are few reports on the hot deformation behavior of Mg-Li-Al-Y alloy and the microstructure evolution during hot tensile deformation.
In this article, the microstructure evolution and deformation mechanism of LAY410 alloy during hot tensile deformation were studied in detail. Furthermore, the fracture mechanisms of LAY410 alloy during hot tensile are discussed.

EXPERIMENT METHOD
Industrial pure Mg (99.95 wt%), Mg-30wt.%Y master alloy, pure Li (99.99wt%), and pure Al (99.99wt%) were used as experimental raw materials. The LAY410 (wt%) alloy was fabricated by melt casting. The concrete melt-casting method is as follows. First, the clean crucible was preheated to 300°C, and then the Mg ingot was placed in the crucible and the temperature was raised to 720°C. After   the Mg ingot was completely melted, the Mg-30wt%Y master alloy and pure Al were successively added into Mg melt, and stirred for 10 min. Subsequently, the temperature was lowered to 680°C and pure Li was added, and stirred for 10 min. After the temperature rose to 740°C, the alloy melt was left standing for 20 min, and the alloy melt was fully stirred and the slag was removed to make the melt clear and clean. Finally, after the melt temperature was further reduced to 710°C, the alloy melt was cast into a mold to obtain a LAY410 alloy ingot. During the whole melting process, sulfur hexafluoride (SF 6 ) and argon (Ar) were used as a mixed shielding gas. Next, the ingot was processed into an extruded billet of Ø44 mm × 40 mm and held at 350°C for 2 h. Then, the four-column hydraulic press (equipment model: IM-Y300) was used to extrude at an extrusion ratio of 13:1 and then air cooling was performed to obtain an extruded bar with a diameter of 12 mm. Finally, the extruded sample was put into a heat treatment furnace at 350°C and then taken out and water cooled immediately after holding for 1 h to prevent the grain growth and retain the grain structure. The alloy was called the LAY410 alloy. The actual chemical composition of the prepared LAY410 alloy tested by inductively coupled plasma optical emission spectrometer (ICP-OES) is shown in Table 1. As can be seen from Table 1, the actual content of each element in the alloy has a small gap with the design composition, indicating that there was almost no element burning loss in the smelting process. After heat preservation for 10 min, the hot tensile test of the LAY410 alloy was carried out at temperatures between 150°C and 300°C with the strain rates from 8 × 10 −5 s −1 to 1.6 × 10 −3 s −1 via an Instron-5982 equipped with a heating device. The tensile specimens were prepared along the extrusion direction (ED), and their dimensions are shown in Figure 1. Furthermore, through scanning electron microscopy (SEM, ZEISS-6035 field-emission) and electron back-scattering diffraction (EBSD) technology, the microstructure of the specimens after hot tensile deformation was analyzed to explore the microstructure evolution during hot tensile deformation.

Analysis of Phases and Microstructures in the Alloy
The X-ray diffraction (XRD) patterns of LAY410 alloy as shown in our previous study   (Figure 2 in this article). There are not only diffraction peaks of the α-Mg phase, but the  diffraction peaks of the Al 2 Y phase as well. There is only one diffraction peak of the Al 2 Y phase with a low intensity in the XRD patterns due to the high solid solubility of Y in Mg (Stanford et al., 2015). Most of the Y atoms are dissolved in the Mg matrix. As a result, the content of Al 2 Y phase formed in LAY410 alloy is very low. In addition, compared with pure Mg, the diffraction peak of the α-Mg phase in LAY410 alloy is shifted to the right, which indicates that the axial ratio value (c/a) of the α-Mg phase decreases as described in our previous study  and the results are reanalyzed in this article. In order to confirm the above conclusions, Jade 6.0 software is used to calculate the lattice constants of the α-Mg phase in pure Mg and LAY410 alloy. The calculated results are shown in Table 2. Compared with pure Mg, the lattice constants a and c of the α-Mg phase in Mg-Li alloy are reduced by 0.29 and 1.41%, respectively. The axial ratio value (c/a) value dropped from 1.624 to 1.605. This is mainly because when the added amount of Li is less than 5.7%, it can be completely dissolved in Mg, thereby reducing the axial ratio value (c/a) of Mg (Koike et al., 2005;Zhou et al., 2017).
To prove the existence of Al 2 Y phase in LAY410 alloy, further, EDS-Mapping of the alloy is carried out as shown in Figure 3. It can be clearly observed from Figure 3 that a small amount of submicron white precipitates are distributed in the grain boundary and grain interior, and the main elements are Al and Y. Combined with the binary phase diagram of Al-Y alloy (Liu et al., 2004), it is concluded that the white precipitated phase in Figure 3A is the Al 2 Y phase. In addition, by observing the transmission electron microscope (TEM) bright-field image of the precipitated phase as shown in Figure 4 and the corresponding selected area electron diffraction pattern (inset), it is further proved that the white particles in the alloy are of the Al 2 Y phase. Because the Al 2 Y phase is a hard phase, it can hinder the dislocation movement. Therefore, the formation of Al 2 Y phase can improve the strength of LAY410 alloy effectively due to dispersion strengthening (Zhao et al., 2016).
In order to determine the grain size and texture type of LAY410 alloy, EBSD technology is used to characterize the alloy. The results are shown in Figure 5. From Figures 5A,B, it can be seen that there are a large number of equiaxed grains in the alloy. And there are many fine recrystallized grains around the equiaxed grains. The average grain size (AGS) is 9.56 μm. As shown in Figure 5C, the misorientation angle of the alloy is mainly high-angle grain boundaries (HAGBs> 10°) with a number fraction of 91.28. This also proves that there is a large amount of DRX in the alloy, which weakens the {0001} basal fiber texture due to forming fresh DRXed grains without a preferred orientation selection. Figure 5D shows the polar figure of the LAY410 alloy, where ED and RD represent the extrusion direction and radial direction, respectively. The pole density points in the {0001} pole figure are distributed along the RD, while the pole density points in the {10-10} and {11-20} pole figures are distributed along the edges. This indicates that there is a weak {0001} basal fiber texture in the alloy, and its texture strength value is 3.82. High-Temperature Mechanical Properties of Alloy The hot tensile true stress-strain curves of LAY410 alloy at the temperatures between 150°C and 300°C with strain rates from 8 × 10 −5 s −1 to 1.6 × 10 −3 s −1 are shown in Figure 6. According to the observation of the curves, the alloy exhibits a typical DRX softening curve. This is mainly due to the decrease in the axial ratio value (c/a) of the alloy, which leads to the increase in dislocation density and the occurrence of cross-slip. It is conducive to nucleation of DRX, causing a certain degree of dynamic softening (Yang et al., 2009;Li et al., 2011). In addition, the strain rate and deformation temperature have a significant effect on the flow stress of the alloy. The flow stress gradually decreases with the strain rate decrease and/or deformation temperature increase. That is, the curves show a positive strain rate effect and negative temperature effect. The change of flow stress curve is mainly manifested in three stages. In the elastic deformation stage, the true-stress increases sharply due to the volumetric expansion, but the shape remaining unchanged of the crystal induced an increase in the distance of atoms in the α-Mg HCP crystal structure (Huang and Huang, 2013). The flow stress rapidly increases to the critical stress. The second stage of tensile deformation is the deformation process in which the flow stress reaches the peak stress from the critical stress after yielding. The strain hardening effect caused by dislocation proliferation is gradually offset by the strain softening effect caused by partial dislocation annihilation. This causes the dislocation propagation rate to decrease gradually until it reaches equilibrium with the dislocation annihilation rate. At this time, the flow stress reaches the peak stress, and the strain hardening rate gradually decreases to zero. The third stage of tensile deformation is the process in which the flow stress gradually decreases until the specimen fracture. As the strain increases, the dislocation annihilation rate is gradually greater than the dislocation propagation rate. It makes the strain softening effect stronger than the strain hardening effect, leading to the strain softening phenomenon in the rheological curve (Xu et al., 2009).

Effect of Deformation Temperature on Microstructure of Alloy
To further investigate the influence of deformation temperatures on the hot tensile deformation behavior of the alloy, the microstructures at the same strain position of the hot tensile specimen are selected for analysis at the same strain rate (ε 1.6 × 10 −4 s −1 ) with different deformation temperatures (T 150°C-250°C). The IPF maps, grain boundary maps, and misorientation angle distribution histograms near the fracture of the hot tensile specimen of LAY410 alloy at the same rate (ε 1.6 × 10 −4 s −1 ) with different deformation temperatures (T 150°C, 200°C, 250°C) are shown in Figure 7. As shown in Figure 7A, when the deformation temperature is 150°C, the microstructures near the fracture of tensile specimen are mainly composed of fine recrystallized grains and coarse deformed grains. The Frontiers in Materials | www.frontiersin.org September 2021 | Volume 8 | Article 714455 5 recrystallized grains are mainly distributed at the trigeminal grain boundaries composed of coarse deformed grains, which show obvious discontinuous dynamic recrystallization (DDRX) characteristics. And the traces of the band-like structure can still be seen in the Figure 7A, indicating that some of the crystal grains are not fully rotated and are still connected in series to show a band-like shape. In addition, there is a clear color orientation gradient inside the coarse crystals, which indicates that there are abundant slip activities inside. The corresponding grain boundary distribution of size angle is shown in Figure 7D, where the green line represents LAGBs and the black line represents HAGBs. It can be seen that a large number of LAGBs are mainly distributed in the coarse grains and at the grain boundaries, which indicates that there are a large number of dislocations inside the coarse deformed grains. As shown in Figure 7G, the corresponding misorientation angle is mainly concentrated in LAGBs with a number fraction of 78.45%. It shows that the microstructures in the Mg alloy are mainly composed of the deformed grains and a few of the recrystallized grains. As the deformation temperature increases to 200°C, the fine recrystallized structure inside the alloy increases slightly, and the grain size is mostly kept between 10 and 20 μm, as shown in Figures 7B,E,H. In addition, the LAGBs inside the coarse deformed grains also decreased slightly, and the corresponding misorientation angle shifted slightly to the HAGBs. When the deformation temperature reaches 250°C, the recrystallized grain structure inside the alloy increases significantly, at this time, HAGBs with a number fraction of 22.90%, as shown in Figures 7C,F,I. The crystal grains rotate and connect to a network and begin to grow up. It is clearly observed that the content of the LAGBs inside the coarse grains is lowered, the number fraction of which is reduced to 56.03%, and the corresponding misorientation angle is obviously shifted to the HAGBs. This is mainly due to the increase in temperature that accelerates the grain boundaries migration and further promotes the occurrence of complete DRX . This leads to an increase in the HAGBs and a decrease in the LAGBs. Small new grains without distortion are produced in the original deformed structure, which makes the overall grain size significantly reduced (Sun et al., 2019).
The distribution of microstructures and the proportion of each microstructure near the fracture of the hot tensile specimens of LAY410 alloy at the same rate (ε 1.6 × 10 −4 s −1 ) with different deformation temperatures (T 150°C, 200°C, 250°C) are shown in Figure 8. The red, yellow, and blue grains in the Figure 8 represent deformed grains, substructure and recrystallized grains, respectively. As shown in Figures 8A-C, when the deformation temperature is 150°C, the microstructures of the alloy are mainly composed of deformed grains and substructures, with a number fraction of 64.2 and 34.0%, respectively. The recrystallized grains accounted for only a small part, with a number fraction of 1.8%. It indicates that DRX is incomplete at 150°C, which is consistent with the results in Figure 7. At 150°C, recrystallization is mainly distributed at the intersection of deformed grains and substructures. This is mainly because not only the grain boundary energy is high at the intersection, but dislocation plugging is prone to occur, resulting in a high dislocation density. Therefore, it is conducive to DRX. Related studies (Malik et al., 2020) have shown that the nucleation temperature of DRX in Mg alloys is about 200°C. In this experiment, the nucleation of DRX can be observed clearly at 150°C, indicating that the decrease in axial ratio value (c/a) is conducive to the nucleation of DRX. As shown in Figures 8D-F, when the deformation temperature reaches 200°C, the deformed grains decrease significantly, and the alloy structure is mainly composed of substructures. The number of dynamic recrystallized grains increased slightly, indicating that the DRX is nucleating and growing. As the diffusion speed between atoms and the migration speed of grain boundaries are accelerated, dislocations are more likely to slip and may be accompanied by cross-slip. Therefore, it is conducive to substructures formation and DRX. When the temperature reaches 250°C, the phenomenon of DRX becomes more obvious and the number increases significantly (see Figures 8G-I). It indicates that when the temperature is higher, the rate of DRX and growth is faster. With the recrystallized grains increase, the softening degree caused by recrystallized grains also increases. Therefore, DRX also contributes to the increase in alloy elongation (Hadadzadeh et al., 2018). In summary, with the deformation temperature increases, the atomic diffusion rate increases. The nucleation and growth of dynamic recrystallized grains are promoted, which leads to flow stress decrease and elongation increase. The kernel average misorientation (KAM) maps near the fracture of the hot tensile specimen of LAY410 alloy at the same rate (ε 1.6 × 10 −4 s −1 ) with different deformation temperatures (T 150°C, 200°C, and 250°C) are shown in Figure 9. KAM value is an indicator of defect density, especially dislocation density, and can indirectly reflect deformation mechanism. It means that the evolution of dislocation density at different temperatures can be reflected in the KAM diagrams during tensile. When the deformation temperature is 150°C, the average KAM value is 1.35, as shown in Figures 9A-C. Meanwhile, the internal stress in the alloy is relatively concentrated, the dislocation density is relatively high, and the internal defects are relatively high. The number of dynamic recrystallized grains is small, and no obvious twins are observed. Therefore, the deformation mechanism is dominated by slippage, and the nucleation of a small number of recrystallized grains plays an auxiliary role in coordinating deformation. As the deformation temperature increases, the average KAM value decreases to 0.58, as shown in Figures 9D-I. Due to the occurrence of DRX, the stress in the alloy is released, and the stress concentration phenomenon is alleviated, resulting in a significant reduction in internal defects of the alloy. There is no obvious stress concentration phenomenon, only a small amount of stress is concentrated at the LAGBs.
The polar figures near the fracture of the hot tensile specimen of LAY410 alloy at the same rate (ε 1.6 × 10 −4 s −1 ) with different deformation temperatures (T 150°C, 200°C, 250°C) are shown in Figure 10. As can be seen from Figure 10A, when the deformation temperature is 150°C, the polar density points of {0001} basal plane are distributed along RD. The polar density points of {11-20} plane and {10-10} plane are distributed along the edge, showing a typical {0001} basal plane fiber texture, that is, the {0001} basal plane of the grains is approximately parallel to the ED, and the texture strength is 9.66. When the deformation temperature reaches 200°C, as shown in Figure 10B, the texture distribution becomes random. The texture strength is reduced, and its value is 8.31, but there is no significant change in the texture type. The increase in temperature is conducive to nonbasal slip system activation, which leads to the randomization of alloy texture and the decrease in texture strength (Barrett et al., 2018). When the temperature reaches 250°C, the texture strength decreases to 3.91, as shown in Figure 10C. The recrystallized grain structure inside the alloy increases significantly, which leads to a significant decrease in the texture strength. In general, the above results indicate that the deformation temperature has a certain influence on the texture strength of the alloy but does not change the texture type. Therefore, the {0001} basal fiber texture of LAY410 alloy still exists after hot tensile deformation.

Hot Tensile Fracture Morphology of Alloy
The fracture morphologies of LAY410 alloy after tensile at the same rate (ε 1.6 × 10 −4 s −1 ) with different deformation temperatures (T 150°C, 200°C, and 250°C) is shown in Figure 11. It can be seen from Figure 11 that the presence of cleavage steps, dimples, and quasi-cleavage tearing ridges leads to uneven fracture after hot tensile, showing the characteristics of ductile-brittle mixed fracture. When the temperature is 150°C, the existence of grain boundary indicates that the fracture mechanism is intergranular fracture. As the temperature reaches 250°C, the number of dimples gradually increases and the color of dimples becomes darker. It shows that the dominated fractures mechanisms of the alloys are gradually transferred from the fragile fracture mechanism to the ductile dimple mechanism due to the stress relaxation and the microstructure homogenization induced by DRX increase as deformation temperatures elevated (Bao et al., 2021).

Hot Tensile Plastic Deformation Mechanism of Alloy
To investigate the plastic deformation mechanism of LAY410 alloy during hot tensile, the Arrhenius model is used to calculate the relationship between various factors and flow stress during deformation. Its specific expression is shown in Eq. 1 (Ji et al., 2011): where _ ε is the strain rate, σ is the flow stress, Q is the activation energy, T is the deformation temperature, R is the molar gas constant, and its value is 8.314 Jmol −1 k −1 . A and n are the material constants, n 1/m. m is the strain rate sensitivity exponent, usually used to reflect the upper limit of the plastic deformation of metal materials.
When the deformation temperature and strain rate are both constants, the strain rate sensitivity exponent can be calculated by Eq. 2 (Huang et al., 2014) as follows: where σ represents the flow stress and _ ε represents the strain rate. When the deformation temperatures are 150°C, 200°C, 250°C, and 300°C, and the strain rates are 8 × 10 −5 s −1 , 1.6 × 10 −4 s −1 , 8 × 10 −4 s −1 , and 1.6 × 10 −3 s −1 , the calculated results are shown in Figure 12. According to the analysis, it is obvious that as the deformation temperature increases from 150°C to 300°C, the m value gradually increases from 0.18 to 0.28. In general, the value of m is used to reflect the ability to inhibit local necking. When the value of m is larger, the ability to inhibit tensile necking is stronger, indicating that the alloy exhibits higher elongation. When m 1, the alloy reaches the ideal infinitely extendable state. Therefore, it can be concluded that LAY410 alloy shows Take the natural logarithm on both sides of Eq. 3 to obtain Eq. 4 which is as follows: Eq. 4 can be further expressed as follows: By linear fitting ln σ − ln 1/T, the relevant slope can be obtained, and the calculation results are shown in Figure 13. Finally, by substituting the values of Q/(R · n) into Eq. 4, it can be obtained that the activation energy of the alloy is about 99.3 kJ/ mol, which is smaller than the lattice diffusion activation energy of pure Mg (135 kJ/mol) and slightly higher than the grain boundary diffusion activation energy of pure Mg (92 kJ/mol) (Atkins, 1984). Therefore, based on the above analysis, it is further proved that the dominant hot tensile deformation mechanism of LAY410 alloy is the alternate coordinated deformation mechanism among GBS, intragranular slip, and DRX. CONCLUSION 1) In the LAY410 alloy, with the strain rate decreases and/or deformation temperature increases, the peak stress gradually decreases and the elongations to fracture gradually increases. In addition, with temperature increases, an obvious steadystate rheological characteristic appears at the later stage of deformation, and the fracture morphology of the alloy gradually transfers from the ductile-brittle mixed fracture mode to the ductile fracture mode.
2) The microstructures of LAY410 alloy are obviously different at varied temperatures. When the deformation temperature is low (T 150°C), the alloy microstructures are mainly composed of deformed grains with LAGBs. There are many internal defects in the alloy, and the recrystallized grains nucleate but the number is small. When the temperature rises to 250°C, the number of recrystallized grains increases significantly, and the DRX is completed more fully.
3) The deformation mechanism of LAY410 alloy is affected by temperature. When the deformation temperature is low, the deformation of the alloy is dominated by slippage, and the twinning plays a supplementary coordinating role. When the deformation temperature is high, DRX plays a dominant role. 4) In the LAY410 alloy, the activation energy of hot tensile deformation is about 99.3 kJ/mol, and the hot tensile deformation mechanism is the alternate coordinated deformation mechanism among GBS, intragranular slip, and DRX.

DATA AVAILABILITY STATEMENT
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

AUTHOR CONTRIBUTIONS
TY wrote and organized the manuscript. JW conducted the final review and revision of the manuscript. ZL and PJ made important contribution in the revision. All authors approved it for publication.

FUNDING
The authors would like to express thanks to the financial support from the Science and Technology Department of Qinghai Province (No. 2018-ZJ-783).