AUTHOR=Zegaer Batool H. , Ioannidis Anastasios , Babis George C. , Ioannidou Vassiliki , Kossyvakis Athanassios , Bersimis Sotiris , Papaparaskevas Joseph , Petinaki Efthimia , Pliatsika Paraskevi , Chatzipanagiotou Stylianos TITLE=Detection of Bacteria Bearing Resistant Biofilm Forms, by Using the Universal and Specific PCR is Still Unhelpful in the Diagnosis of Periprosthetic Joint Infections JOURNAL=Frontiers in Medicine VOLUME=1 YEAR=2014 URL=https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2014.00030 DOI=10.3389/fmed.2014.00030 ISSN=2296-858X ABSTRACT=
Intraoperative conventional bacteriological cultures were compared with different polymerase chain reaction (PCR) methods in patients with total joint arthroplasties. The isolated bacteria were investigated for biofilm formation, and the biofilm forming strains, in their planktonic and biofilm forms, were further tested for their antimicrobial resistance against several clinically important antimicrobials. Forty four bone and joint samples were included and classified as infected or non-infected according to standard criteria for periprosthetic hip and knee infections. For the bacteriological diagnosis, conventional culture, two types of universal PCR and species specific PCR for three selected pathogens (