%A Kolb,Manon %A Lazarevic,Vladimir %A Emonet,Stéphane %A Calmy,Alexandra %A Girard,Myriam %A Gaïa,Nadia %A Charretier,Yannick %A Cherkaoui,Abdessalam %A Keller,Peter %A Huber,Christoph %A Schrenzel,Jacques %D 2019 %J Frontiers in Medicine %C %F %G English %K Next-generation sequencing,culture-negative endocarditis,clinical metagenomics,cardiobacterium hominis,diagnosis %Q %R 10.3389/fmed.2019.00203 %W %L %M %P %7 %8 2019-September-20 %9 Case Report %# %! Clinical metagenomics diagnosis of challenging endocarditis %* %< %T Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis %U https://www.frontiersin.org/articles/10.3389/fmed.2019.00203 %V 6 %0 JOURNAL ARTICLE %@ 2296-858X %X Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. Mapping of the sequence reads against reference genomic sequences, a 16S rRNA gene database and clade-specific marker genes suggested an infection caused by Cardiobacterium hominis.