Real-world evidence of survival benefit of remdesivir: study of 419 propensity score-matched patients hospitalized over the alpha and delta waves of COVID-19 in New Orleans, LA

Background The direct acting antiviral remdesivir (RDV) has shown promising results in randomized clinical trials. This study is a unique report of real clinical practice RDV administration for COVID-19 from alpha through delta variant circulation in New Orleans, Louisiana (NOLA). Patients in NOLA have among US worst pre-COVID health outcomes, and the region was an early epicenter for severe COVID. Methods Data were directly extracted from electronic medical records through REACHnet. Of 9,106 adults with COVID, 1,928 were admitted to inpatient care within 7 days of diagnosis. The propensity score is based upon 22 selected covariates, related to both RDV assignment and outcome of interest. RDV and non-RDV patients were matched 1:1 with replacement, by location and calendar period of admission. Primary and secondary endpoints were, death from any cause and inpatient discharge, within 28 and 14 days after inpatient admission. Results Of 448 patients treated with RDV, 419 (94%) were successfully matched to a non-RDV patient. 145 (35%) patients received RDV for < 5 days, 235 (56%) for 5 days, and 39 (9%) for > 5 days. 96% of those on RDV received it within 2 days of admission. RDV was more frequently prescribed in patients with pneumonia (standardized difference: 0.75), respiratory failure, hypoxemia, or dependence on supplemental oxygen (0.69), and obesity (0.35) within 5 days prior to RDV initiation or corresponding day in non-RDV patients (index day). RDV patients were numerically more likely to be on steroids within 5 days prior to index day (86 vs. 82%) and within 7 days after inpatient admission (96 vs. 87%). RDV was significantly associated with lower risk of death within 14 days after admission (hazard ratio [HR]: 0.37, 95% CI: 0.19 to 0.69, p = 0.002) but not within 28 days (HR: 0.62, 95% CI: 0.36 to 1.07, p = 0.08). Discharge within 14 days of admission was significantly more likely for RDV patients (p < 0.001) and numerically more likely within 28 days after admission (p = 0.06). Conclusion Overall, our findings support recommendation of RDV administration for COVID-19 in a highly comorbid, highly impoverished population representative of both Black and White subjects in the US Gulf South.

Background: The direct acting antiviral remdesivir (RDV) has shown promising results in randomized clinical trials.This study is a unique report of real clinical practice RDV administration for COVID-19 from alpha through delta variant circulation in New Orleans, Louisiana (NOLA).Patients in NOLA have among US worst pre-COVID health outcomes, and the region was an early epicenter for severe COVID.
Methods: Data were directly extracted from electronic medical records through REACHnet.Of 9,106 adults with COVID, 1,928 were admitted to inpatient care within 7 days of diagnosis.The propensity score is based upon 22 selected covariates, related to both RDV assignment and outcome of interest.RDV and non-RDV patients were matched 1:1 with replacement, by location and calendar period of admission.Primary and secondary endpoints were, death from any cause and inpatient discharge, within 28 and 14 days after inpatient admission.
Results: Of 448 patients treated with RDV, 419 (94%) were successfully matched to a non-RDV patient.145 (35%) patients received RDV for < 5 days, 235 (56%) for 5 days, and 39 (9%) for > 5 days.96% of those on RDV received it within 2 days of admission.RDV was more frequently prescribed in patients with pneumonia (standardized difference: 0.75), respiratory failure, hypoxemia, or dependence on supplemental oxygen (0.69), and obesity (0.35) within 5 days prior to RDV initiation or corresponding day in non-RDV patients (index day).RDV patients were numerically more likely to be on steroids within 5 days prior to index day (86 vs. 82%) and within 7 days after inpatient admission (96 vs. 87%).RDV was significantly associated with lower risk of death within 14 days after admission (hazard ratio [HR]: 0.37, 95% CI: 0.19 to 0.69, p = 0.002) but not within 28 days

Introduction and background
SARS CoV-2 variants will likely circulate for years to come, underscoring an ongoing need for adequate guidelines and effective treatments independent of virus immunogenic shifts.To contain COVID-19 most effectively moving forward, a combined strategy of prevention (vaccines, intermittent masks, social distancing) and treatments [anti-inflammatory drugs, direct acting antivirals (DAAs), host directed antivirals (HDAVs), and passive immunotherapy in the form of antibodies] is needed (1,2).Because of the extreme social vulnerability and health inequity among US populations, regionally focused Real-World Experience (RWExp) studies could inform local guidelines.A summary of clinical trials, observational studies, and the guidelines of the Infectious Diseases Society of America (IDSA) is provided to walk the reader out of the maze of conflicting results and guidelines released around use of RDV in COVID-19 patients and to provide rationale for the current study.Supplementary Table 1 provides the main characteristics and links to RDV randomized clinical trials (RCT) and retrospective studies, and Supplementary Table 2 provides IDSA as well as World Health Organization (WHO) and Food Drug Administration (FDA) guidelines for the use of RDV in COVID-19 mentioned in the next paragraphs, reported in the framework of COVID-19 waves, study RDV/non-RDV patient ratios and state's vaccination of LA population over the study period (Figure 2).Pre-clinical phase studies: The direct acting antiviral RDV, or GS-5734, is a ribonucleotide analog inhibitor of viral RNA-dependent RNA polymerase.Rationale for the use of RDV for SARS CoV-2 came from in vitro and in vivo efficacy against Middle Eastern Respiratory Syndrome (MERS) CoV (3)(4)(5)(6)(7), in vitro activity against SARS CoV-2 (8) and in vivo efficacy of SARS CoV-2 in non-human primate studies (6, 9, 10).
Early clinical trial, funded by the Chinese Academy of Medical Sciences Emergency Project of COVID-19: Early in the pandemic, from February to March of 2020, Wang et al. (11) performed a randomized, double-blind, placebo-controlled, multicenter trial at ten hospitals in Hubei, China to evaluate RDV in inpatients with COVID-19 (11).They compared 158 patients assigned to RDV to 79 patients assigned to placebo and found that RDV use was associated with numerically faster time to clinical improvement versus placebo among patients with symptom duration of 10 days or less, but this difference was not statistically significant.It is important to note that the study was underpowered due to enrollment challenges.
Early randomized clinical trial [ACTT-1] and study of compassionate use funded by Gilead Sciences, Inc.: In the early trial by Grein et al. (12) RDV under compassionate administration was beneficial in 53 patients with severe COVID-19.The preliminary results of the first randomized trial in the US, the ACTT-1 study, released in May 2020 in the COVID-19 pandemic, found RDV as a potentially useful therapeutic with favorable toxicity profile (13).
In the ACTT-1 study, 541 patients were assigned to RDV and 521 to placebo, enrolled from February to April 2020 (Supplementary Table 1).The ACTT-1 population was 20% black, and 55% of participants had two or more coexisting conditions.ACTT-1 found that RDV was superior to placebo in shortening time to recovery in adults hospitalized with COVID-19 and with evidence of lower respiratory tract infection.The final result was communicated in October 2020 leading to RDV FDA Emergency Use Authorization (EUA) (14).Subsequent randomized clinical trials, SIMPLE and PINETREE, funded by Gilead Sciences, Inc.: The SIMPLE-Severe, (results released 1 June 2020) (15) trial compared 5 versus 10 days RDV in a randomized, open-label phase 3 trial of hospitalized patients with ambient air oxygen saturation ≤ 94% and radiologic evidence of pneumonia, finding no significant difference in 5 versus 10 day course for non-ventilated patients.SIMPLE-Moderate (16) was a randomized, open-label trial of 584 hospitalized patients with moderate COVID-19 pneumonia (pulmonary infiltrates and roomair oxygen saturation > 94%) enrolled from March 15 through 18 April 2020, at 105 hospitals in the United States, Europe, and Asia.SIMPLE-Moderate found that patients randomized to a 5day, but not a 10-day, course of RDV had significant improvement in clinical status as measured on day 11.The later PINETREE study, released September 2021, showed that early treatment with three days of RDV prevented progression to severe COVID-19 in outpatients, including a population that was 7% black, 62% diabetic, and 55% obese (17).Two studies in April-May 2020 were combined to assess open label RDV: GS-5773 study (SIMPLE-Severe; oxygen ≤ 94, pulmonary infiltrates) and GS-5807 (a real-world control cohort).Because GS-5773 did not have a no treatment control arm, RDV efficacy was assessed by comparing results between 5,773 and 5,807 (no treatment), and this comparison found some survival benefit in RDV open label treatment group compared to the real-world control study group (18).Randomized Clinical Trials, SOLIDARITY, sponsored by the WHO: The World Health Organization (WHO) led the SOLIDARITY clinical trial, presented as interim data in November 2020 (19) then final data in 2022 (20).SOLIDARITY is a multicenter international randomized adaptive clinical trial sponsored by the WHO to determine anti-COVID efficacy of multiple drugs selected over time.At various stages of the study, RDV has been compared to hydroxychloroquine, lopinavir, interferon β1a, or the tyrosine kinase inhibitor acalabrutinib, versus local standard of care, in patients hospitalized for SARS-CoV-2 infection.Outcome assessed is all-cause mortality, stratified by severity of disease at time of randomization.Major secondary outcomes are duration of hospital stay and time to ventilation or intensive care.Trial enrollment was from March 2020 to November 2021, with only RDV continued through the end of enrollment and with inability to show efficacy of the other agents.Overall, 4,146 subjects were assigned to RDV.Interim results did not show a benefit of RDV administration.The Canadian Treatments for COVID-19 trial (CATCO) reported in January 2022 a lower mortality in RDV patients across 52 Canadian hospitals participating in the SOLIDARITY trial (21).Final SOLIDARITY results, released May 2022 and including the last trial phase where the only drug randomized was RDV, showed a small decrease in mortality among adults requiring oxygen but not ventilated (RDV 14.6% vs. control 16.3%; RR 0.87 [95% CI 0.76-0.99],p = 0.04) and lower progression to mechanical ventilation and / or death (23.7% vs. 27.1%;RR 0.83 [0.75-0.93],p = 0.001), in RDV versus non-RDV local standard of care control subjects.DisCoVeRy study group, funded by the European Union Commission and others was a Phase 3 RCT open-label intention-to-treat population study across 48 sites in the E.U. with primary outcome being the day-15 clinical status measured by the WHO seven-point ordinal scale.The findings, released in February 2022 (22), report no clinical benefit from the use of RDV for inpatients symptomatic for more than 7 days, and who required oxygen support.
Real world experience / retrospective observational studies, non-Gilead miscellaneous funding: Multiple real world efficacy studies of RDV have been published to date (23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36) (Supplementary Table 1).Overall, clinical trial consensus at this time seemed to be that RDV has some effect in shortening time to clinical improvement (24,37), shortening duration of admission, preventing progression to mechanical ventilation, and some effect in reducing mortality.Although often qualified as mild, the optimal effect of RDV seems to be achieved when drug is provided at an early phase of the disease, e.g., at time of milder clinical severity with benefit driven by patients on no or low-flow oxygen, with full dose completion.Benefits appear most likely when used in combination with steroids, though RDV benefits appear to be driven at least in part by RDV itself and not by concomitant antiinflammatory therapies (37).Additional review and meta-analysis were provided sponsored by the American College and Physicians Practice Points (38).
This study aimed to compare clinical outcomes between patients who received RDV and those who did not in the population of greater urban NOLA, a region with some of the worst pre-COVID health outcomes in the US and an early epicenter for severe COVID.

Study design and population
In this retrospective study, data were collected from electronic health records, in MediTech at Tulane Medical Center, TMC, and EPIC at University Medical Center, UMC, two academic medical centers located in New Orleans, Louisiana.The data were collected prospectively from March 2020 to September 2021, from adult patients aged ≥ 18 admitted to inpatient care within 7 days of the first COVID-19 diagnosis.The target variable is RDV treatment vs. non treatment.Patients who started RDV before inpatient admission or > 3 days after inpatient admission were not included in the analysis.Whether a patient was started immediately on RDV or as late as 3 days following admission was up to provider discretion.Patients started on RDV more than 3 days following admission were excluded because they would have been likely to be matched to patients healthy enough to be discharged shortly after the index day, thus not qualifying as candidates for RDV treatment.All patients in the study were inpatients, with RDV recommended for 5-day therapeutic course (vs.3-day preventive course for outpatients).Whether a full 5day course was administered, or the patient was discharged prior to 5 doses (due to clinical improvement) was based on provider discretion, as was standard of care during the study timeframe.Since this study is retrospective and population-based (for the two institutions involved, from 3/2020 to 9/2021, following the natural progression of the pandemic), there was no prior determination of sample size.All patients admitted to inpatient care on the day of first COVID-19 diagnosis and starting remdesivir within 3 days after inpatient admission were included in the crude analysis.This resulted in a sample size of 448 participants.The prep-to-research queries, listed in Supplementary Table 3, included demographics, health conditions and diseases, diagnoses, medical interventions, medications administered, laboratory results, vital signs, inpatient admission information, and mortality information.When available, data were collected from 6 months prior to the patient's COVID-19 diagnosis to 9 months thereafter, allowing estimation of baseline health conditions, calculation of the Charlson comorbidity index and information on vital status post COVID-19 diagnosis.Data were stored in the PCORnet Common Data Model format, version 6.0 (2018) (39).

Endpoints
The primary endpoint was death from any cause within 28 days after inpatient admission.Secondary endpoints were death from any cause within 14 days after inpatient admission and inpatient discharge within 14 days and within 28 days after admission.

Matching and statistical analyses
Each endpoint was analyzed both in all patients (crude analysis) and in matched patients (matched sample analysis).Exact matching was performed on location of inpatient admission (University Medical Center; Tulane Medical Center) and on calendar period 10.3389/fmed.2024.1390164 of inpatient admission [March-May 2020 (predominant circulation of the alpha variant, first wave/peak of mortality); June-August 2020 (alpha variant, second wave); September 2020-February 2021 (alpha variant third wave); March-May 2021 (last period of alpha variant predominance); June-September 2021 (predominant circulation of the delta variant)], followed by propensity score matching based on 22 potentially prognostically important baseline covariates listed in Supplementary Table 4. Covariates include select demographics, pre-COVID medical conditions, and clinical status/treatment within 5 days prior to RDV (or corresponding day in patients who did not receive RDV).The matching was performed 1:1 with replacement using the greedy algorithm and a caliper distance of 0.2 of the standard deviation of the logit of the propensity score.To minimize the number of non-RDV patients being matched to ≥ 2 RDV-patients, the following selection process was used: (1) for each RDV-patient, select the non-RDV patient with the smallest caliper distance; (2) if this patient was previously selected for matching, select the patient with the next smallest caliper distance; if all patients within the caliper distance were previously selected for matching, select the non-RDV patient with the smallest caliper distance.Each RDV patient was matched to only one non-RDV patient.However, matching was performed with replacement, i.e., a non-RDV patient could be matched to more than one RDV patient.This is because if matching was performed without replacement, many RDV patients would not have been successfully matched to a non-RDV patient and thus would have been excluded from the analysis.To prevent immortal time bias, a non-RDV patient could be matched to an RDV patient only if the non-RDV patient was still in inpatient care on the index day, defined as the day when the RDV patient initiated remdesivir relative to admission day.The survival time outcome is measured from patient admission, so time prior to patient admission is not included in days of survival outcome.Related to actions preceding admission, all healthrelated covariates used for the propensity score matching took into account the period preceding admission: (1) All components of the Charlson comorbidity index were assessed "based on past or ongoing medical conditions at baseline, " (2) All other healthrelated covariates (oxygenation level, pneumonia, sepsis, obesity, concomitant medications) were assessed "on or within 5 days prior to day of RDV initiation or corresponding day in patients who did not receive RDV." Therefore, they cover at least the 2 days preceding admission (if RDV was initiated 3 days after admission) and at most the 5 days preceding admission (if RDV was initiated on the day of admission).Adequacy of the matching was assessed by standardized differences.Cumulative probabilities of death from any cause within 28 days and within 14 days after inpatient admission-including deaths occurring after dischargewere estimated using the Kaplan-Meier method.In the crude analysis, the association between RDV exposure and death from any cause was assessed using standard Cox proportional hazards regression models.In the matched sample analysis, it was assessed using Cox proportional hazards regression models with shared frailty and a robust sandwich-type variance estimator to account for clustering within matched pairs, clustering within participants and clustering in the cross-classification of these two types of clusters (40,41).In the matched sample analysis, the Cox proportional hazards regression models were adjusted for all baseline covariates with an absolute standardized difference ≥ 0.15 in at least one category.For all survival analyses, patients discharged before the cutoff day (Day 14 or Day 28) were censored on the cutoff day if they had available medical records after the cutoff day, otherwise on the discharge day.The proportional hazards assumption of each Cox model was assessed graphically using a log-log plot The associations between exposure to RDV and inpatient discharge within 14 days and within 28 days after admission were assessed using Fisher '

Study population
A total of 448 patients who received RDV and 1,438 patients who did not receive RDV were included in the crude analysis and considered for inclusion in the matched sample analysis (Figure 1).Inpatient admission dates ranged from 12 March 2020 to 20 September 2021.Baseline characteristics of the study population in original and matched samples (Table 1) and in matched and unmatched samples (Table 2) are presented.In the original sample, 1,029 (55%) patients were male, 1,074 (57%) were Black (African American), and the median age was 57 years (interquartile range [IQR]: 43 to 68).The median of the Charlson comorbidity index was 4 (IQR: 2 to 6).Within 5 days prior to the index day, 297 (16%) patients were intubated, and 664 (35%) had respiratory failure, hypoxemia, or dependence on oxygen (Table 1).The study ratio of RDV/non-RDV patients is reported over time (Figure 2).To provide the context in which the ratio evolved, the main milestones relevant to RDV use, pre-and post-RDV FDA EUA, are visually reported in Figure 2 including the release of major randomized clinical trials conclusions, guidelines for the use of RDV in COVID-19 of IDSA, WHO, and FDA.To provide context, official state population data for death related to COVID-19 by variant waves and rate of vaccination is provided in the same graph (Figure 2).The rapid increase of RDV administration reached a 65% peak ∼10 months after FDA EUA, over the 3rd alpha wave and stabilized to ∼50% over the delta wave (Figure 2 orange curve).RDV was more frequently administered to patients with obesity (standardized difference: 0.35) (Table 1).Within 5 days prior to index day, the concomitant administration of steroids was more frequent (standardized difference: 0.88), and patients more likely to receive RDV if experienced pneumonia (0.75), respiratory failure, hypoxemia or were on supplemental oxygen but not intubated (0.69) (Table 1).Of the 448 RDV-patients, 419 (94%) were successfully matched to a non-RDV patient.Of the 1,438 non-RDV patients, 220 were matched to at least one patient who received RDV: 145 (66%) were matched to one RDV patient, 40 (18%) to two, 6 (3%) to three and 29 (13%) to more than three (up to 13).The median absolute standardized difference in the matched sample was 0.06 (IQR: 0.01 to 0.11).Of the 419 RDV patients, 145 (35%) received RDV for less than 5 days, 235 (56%) for 5 days, and 39 (9%) for more than 5 days (Table 3).Steroids were concomitant prescribed within 7 days of admission to 401/419 (96%) RDV-patients and 366/419 (87%) non-RDV patients (Table 3).

Patient outcome over time
The proportions of patients discharged, still admitted, and who died during inpatient stay are described over time in Figure 3 in (a) crude and (b) matched sample analysis.

Death from any cause within 14 and 28 days of admission
Results for mortality endpoints from the Cox proportional hazard regression models are provided in Figure 4.In the matched sample analysis, patients who received RDV were significantly less likely to die within 14 days after inpatient admission (hazard ratio [HR]: 0.37, 95% CI: 0.19 to 0.69, p = 0.002) but not within 28 days after inpatient admission (HR: 0.62, 95% CI: 0.36 to 1.07, p = 0.08) after adjustment for the six baseline covariates with an absolute standardized difference ≥ 0.15 in at least one category (race, location of residence, HIV/AIDS, oxygenation level, sepsis and anticoagulants).The results of the subgroup analysis of death from any cause within 28 days after inpatient admission are provided in Table 4.In the matched sample analysis, exposure to RDV was significantly associated with a lower risk of death in patients admitted between March and May 2021 (HR: 0.11, 95% CI: 0.01 to 0.95, p = 0.04) or with no sepsis on or within 5 days prior to index day (HR: 0.39, 95% CI: 0.17 to 0.93, p = 0.03).In the matched sample analysis, co-administration of RDV and steroids was not associated with the risk of death (Table 4).Kaplan-Meier estimates of the cumulative probability of death from any cause within 28 days after admission, in crude and matched samples, are provided in Figure 5.

Inpatient discharge
Results for the inpatient discharge endpoints are provided in Table 5.In the matched sample analysis, patients who received RDV were significantly more likely to be discharged within 14 days after admission (p < 0.001) and numerically more likely to be discharged within 28 days after admission (p = 0.06).

Discussion
In this study of 419 propensity score-matched adult patients hospitalized with COVID-19 in the NOLA region, patients who received RDV were significantly less likely to die within 14 days after inpatient admission (hazard ratio [HR]: 0.37, 95% CI: 0.19 to 0.69, p = 0.002) and numerically-but not significantly-less likely to die within 28 days after inpatient admission (HR: 0.62, 95% CI: 0.36 to 1.07, p = 0.08).These findings are consistent with those observed in Beigel et al. (13) by day 15 (HR: 0.55, 95% CI: 0.36 to 0.83) and day 29 (HR: 0.73, 95% CI: 0.52 to 1.03) (13).Interestingly, in the current study exposure to RDV was significantly associated with lower risk of death from any cause within 28 days (Table 4) for patients, 1-who did not experience sepsis (on or within 5 days prior to index day) (HR: 0.39, 95% CI: 0.17 to 0.93, p = 0.03) and 2-for patients admitted between the 3rd alpha wave and the delta wave, March to May 2021 (HR: 0.  1).
The higher estimate in our study could be explained by the delta variant: all RCTs cited above were conducted before the emergence of the delta variant, and we observed a higher risk of death during delta variant-predominant circulation than during any alpha variant wave.The high cumulative probability of death we observed did not seem to be explained by severity of COVID-19 at baseline, as, for example, baseline proportions of patients intubated and dependent on supplemental oxygen were lower than in Beigel et al. (13) and Ader et al. (22) (Supplementary Table 1).Other potential contributors to the high death rate in our cohort include (1) an overall high rate of sepsis as well as (2) the high pre-COVID morbidity in the NOLA region, among US worst for most health metrics.These two factors could also be related to each other, with pre-COVID comorbidities such as obesity and diabetes increasing the likelihood of peri-COVID complications.
Patients who received RDV were significantly more likely to be discharged within 14 days (72 versus 57%, p < 0.001) (Table 4) and only numerically but not significantly more likely within 28 days (80 vs. 74%, p = 0.06).The proportion of patients discharged within 14 days among those who did not receive RDV (57%) was higher than that observed in the control group of Wang et al. (11)   Remdesivir administration ratio and daily death 7-day moving average over time in the United States.Patient outcome over time in (A) crude analysis and (B) matched sample analysis.
Death from any cause within 28 days and within 14 days after inpatient admission.In the crude analysis, the association between RDV exposure and death from any cause was assessed using standard Cox proportional hazards regression models.In the matched sample analysis, it was assessed using Cox proportional hazards regression models with shared frailty and a robust sandwich-type variance estimator to account for clustering within matched pairs, clustering within participants and clustering in the cross-classification of these two types of clusters.The proportional hazards assumption of the Cox models was met for the 14-day crude and matched sample analyses, but not for the 28-day crude and matched sample analyses.Although studies encountering non-proportional hazards are common, effectively addressing non-proportional hazards remains an ongoing research topic.Despite the proportional hazards assumption not being met for our 28-day analyses, we are following the recommendation from the FDA-initiated "Non-Proportional Hazards Cross-Pharma Working Group" to report hazard ratio estimates from these models: "In terms of summarizing treatment effect, researchers should continue to present Kaplan-Meier curves and Cox model hazard ratio estimates" (45).
region of interest for clinical trials due to its high concentration of high social vulnerability.Data on expensive treatments that work in a bicoastal population with relatively intense healthcare access may not necessarily be transposable to the Gulf South population or even the more neglected pockets (e.g., the South Bronx) within well-resourced US regions.Health disparities observed in COVID-19 were reflective of competitive clinical trial access patterns in the US and highlight an opportunity for improvement.
This RWE of RDV effectiveness is also unique in corroborating the overall effectiveness of RDV, here in crude and matched analysis upon survival within 28 days of admission.
Among the 22 co-variates used for PS calculation, the Charlson comorbidity index (CCI) is quite low.Although the cohort's patients display a high prevalence of co-morbidities associated with risk for severe COVID-19 outcomes, the CCI may not reflect optimally the extent to which poor patients of relatively young age may be affected.The CCI is a commonly used index but is limited for prediction of risk in patients with acute COVID-19: the weight attributed to some criteria such as 1-HIV is excessive, outdated, as not reflecting the near cure benefit from Anti Retro Viral (ARV) in multi-therapy, 2-cancers are much less associated with immunosuppression depending upon the type solid vs. hematologic, and time from last therapy, 3-the weight of age is not adapted to our population as accumulation of advanced health issues at young age is not incremented, 4-criteria of major significance such as obesity, substance use disorder, effect of co-morbidities clustering and vaccination status are not included in the CCI.In addition, the comparison from one study to another is difficult as multiple versions of the CCI are used (44).We observe unique characteristics of RDV prescription in RWE over several waves of the pandemic.First, a rapid increased proportion of inpatients prescribed RDV, with a peak RDV/non-RDV inpatients ratio > 50% by the 3rd alpha wave (September 2020-April 2021) and over the delta wave (July-September 2021) (orange curve, Figure 2).This curve paralleled with LA population vaccination coverage as ∼ 50% of the population had completed full vaccination by July 2021 (green curve, Figure 2).In our clinical experience, however, the actual proportion of hospitalized vaccinated patients was low.Second, RDV was prescribed more if patient was obese, had pneumonia, required supplemental oxygen (but not intubation), and was prescribed steroids within 5 days prior to index day (58 vs. 19%) (Table 1).
The rapid increase of patients (absolute and proportion) who received RDV (Figure 2, orange curve) likely reflects wider availability of the drug after Emergency Use Authorization (EUA) by FDA and increased confidence toward benefit attributed to RDV, especially after ACTT-1 study and FDA final approval in September-October 2020.The graph suggests that the interim WHO guidelines based the SOLIDARITY trial against the use of RDV in COVID-19 released in November 2020, did not stop the prescription of RDV.Both study sites (TMC and UMC NOLA) endorsed the guidance from the EUA for RDV administration: significant COVID-19 illness with SpO2 ≤ 94% on room air or required supplemental oxygen, invasive mechanical ventilation, and alanine aminotransferase (ALT) less than five time the upper limit of normal.However, the decision to prescribe RDV was made by the attending physician for each individual patient.The inflection in the proportion of RDV administration, from 64% at the peak of 3rd alpha wave to 54% at the peak of the delta wave, may be explained by the release of the IDSA guidelines vs. 4.3 in June 2021 suggesting against the administration of RDV in patients with requirement for MV (Figure 2 and Supplementary Table 2, IDSA 4.3 recommendation # 10b).
Our study has several limitations, mostly inherent to the aim of focusing on a specific region's underrepresented population, driving the number of recruits down.It is possible that scientific and public debates regarding RDV benefit, risk, and cost had an unmeasured influence on provider use of RDV at various points in the pandemic.Specifically, an unscalable possible bias of our study is related to the consequences of the release of the interim SOLIDARITY results upon local physician's confidence toward RDV administration and benefit.It was noticeable, however, from direct interactions at patients' bedside, files reviews and professional meetings/interactions, that while most physicians were compliant with national IDSA recommendations, some prioritized WHO guidelines.This dual perception regarding RDV administration may have ultimately optimized the proportion of matching control patients.Lack of control for this influence is a limitation of this study and something that might be anticipated and explored in future public health crises.The vaccination status was not available for most patients in the EMR and the only reportable rate was taken from state statistics.Our study population with higher co-morbidities and low healthcare access had consistently low vaccination coverage on admission (ClinSeqSer cohort, unpublished data).
Strengths of the study include stable oxygen supply, the inclusion of two systems of care, one private and the other a safety net non-profit, public hospital (UMC), that shared standard of care, scheduled consensus meetings, where physicians are rotating in both centers but with dissimilar patients on admission (by primary payer, proportion of homeless population, ZIP codes), while spanning over multiple peaks of death over the waves of alpha variants over the delta variant and in the interim period.
Compared with multicenter studies and larger cohorts, the relative lower number of recruits are compensated by the strengths of focusing the study on a single homogeneous population, in a specific region, over a non-previously reported period extending from the early phase to the delta wave in the summer of 2021.Our study is unique as it shows that RDV has benefit in populations that have been underrepresented in clinical trials and is consistent with observation provided in the study by Garibaldi et al. (25).

Conclusion
This study suggests that in a cohort representative of the greater urban NOLA region, RDV was associated with a survival benefit, especially in non-sepsis patients, and more likely discharge to home within 14 (albeit not 28) days of admission for acute COVID-19 treatment.This study corroborates the overall findings regarding RDV efficacy, here mostly in association with steroids.The finding that RDV treatment is associated with more likely discharge within 14, but not 28, days, raises the possibility that RDV is protective against early-phase virus-mediated pathology, but not the later-phase inflammatory pathology of COVID.We conclude that use of RDV for COVID-19 is supported by this data, but must be paired with ongoing study of evolving recommendations for late-COVID steroids and other potential immunomodulators.This study is unique for several reasons: 1its representation of both White and Black patients living in a high state of impoverishment, with advanced pre-COVID-19 health issues and relatively low vaccination coverage, 2-describing RDV effect over the circulation of two major SARS CoV-2 variant circulation periods with deadly outcomes (1st to 3rd alpha waves and the delta wave), and 3-by its illustration of characteristics of RDV prescription in obese subjects, patients with pneumonia and patients with dementia.In conclusion, both small benefit on survival and on admission length are cumulatively valuable and remarkable in this impoverished population where severity of chronic health problems is correlated with expectation of high risks for acute respiratory viral infection and high death rate.Importantly, our real-world experience shows no noticeable inequity in RDV administration as benefits were observed with similar effectiveness of RDV for acute COVID-19 regardless of race, sex, ZIP code, primary payer, or medical center/health admission.This study reflects the remarkable effectiveness and dedication of the healthcare workers over the pandemic in LA, as well as the long road ahead to improved baseline health, across races, in the region.R , the National Patient-Centered Clinical Research Network, which was developed with funding from PCORI R .2-the Gilead Science study group: Gilead Sciences, Inc. participated in discussions about milestones advance, in quarterly study team calls.Gilead Science members did not receive raw data or influence the analysis strategy or study design.Special thanks to Janelle Molina, Nini Estevez, and Hong Hua.

FIGURE 1
FIGURE 1 Study flow diagram.

FIGURE 2
FIGURE 2 10.3389/fmed.2024.1390164 s exact test in the crude analysis and McNemar's test in the matched sample analysis.Of note, McNemar's test does not account for matching with replacement, thus p-values should be interpreted with caution.The matching and statistical analyses were performed using Stata version 16.1 (Stata Corp., College Station, TX) and R version 4.3.0.

TABLE 1
11, 95% CI: 0.01 to 0.95, p = 0.04) though n was Baseline characteristics of the study population in the original and matched samples.Oxygenation level on or within 5 days prior to day of RDV initiation or corresponding day in patients who did not receive RDV* (16)or within 5 days prior to day of admission in patients in the original sample who did not receive remdesivir.small in this subgroup (n = 22 RDV, n = 22 no-RDV).RDV was not associated with a significantly lower risk of 28-day mortality in other calendar periods or in any of the baseline COVID-19 severity subgroups.In Beigel et al.(13), the only baseline observed in the control group of all previous RCTs in hospitalized patients with COVID-19: 15.6% in the WHO Solidarity trial, 2% in Spinner et al.(16), 9% in Ader et al. (22), 13% in Wang et al. (11) and 15.2% in Beigel et al. (13) (Supplementary Table

TABLE 3
Exposure to remdesivir and concomitant steroids in the original and matched samples.
Day of RDV initiation or corresponding day in patients who did not receive RDV after match).In contrast, the cumulative probability of death in White patients (27% in RDV and 12% in non-RDV) and in Black patients (23 and 23%) is closer to that of the Ochsner cohort, though over a much longer period in the present study.Unfortunately, other comparator studies are not available, as in the US, clinical trial activity frequently concentrates in bicoastal states, neglecting middle regions including the impoverished Gulf South.The Gulf South should be perceived as a valuable US 10.3389/fmed.2024.1390164FIGURE 3

TABLE 4
Death from any cause within 28 days after inpatient admission in each stratum of the baseline covariates used for the propensity score matching.Oxygenation level on or within 5 days prior to day of RDV initiation or corresponding day in patients who did not receive RDV *Indicates that these components e.g., HIV/AIDS were part of the Charlson's comorbidity index.FIGURE 5Kaplan-Meier estimates of the cumulative probability of death from any cause within 28 days after inpatient admission in (A) crude analysis and (B) matched sample analysis.

TABLE 5
Inpatient discharge within 14 days and within 28 days after admission.