AUTHOR=Flint Annika , Butcher James , Clarke Cyril , Marlow Denver , Stintzi Alain 

TITLE=Use of a Rabbit Soft Tissue Chamber Model to Investigate Campylobacter Jejuni–Host Interactions

JOURNAL=Frontiers in Microbiology

VOLUME=1

YEAR=2010

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2010.00126

DOI=10.3389/fmicb.2010.00126

ISSN=1664-302X

ABSTRACT=<p>Despite the prevalence of <italic>Campylobacter jejuni</italic> as an important food borne pathogen, the microbial factors governing its infection process are poorly characterized. In this study, we developed a novel rabbit soft tissue chamber model to investigate <italic>C. jejuni</italic> interactions with its host. The <italic>in vivo</italic> transcriptome profile of <italic>C. jejuni</italic> was monitored as a function of time post-infection by competitive microarray hybridization with cDNA obtained from <italic>C. jejuni</italic> grown <italic>in vitro</italic>. Genome-wide expression analysis identified 449 genes expressed at significantly different levels <italic>in vivo</italic>. Genes implicated to play important roles in early colonization of <italic>C. jejuni</italic> within the tissue chamber include up-regulation of genes involved in ribosomal protein synthesis and modification, heat shock response, and primary adaptation to the host environment (DccSR regulon). Genes encoding proteins involved in the TCA cycle and flagella related components were found to be significantly down-regulated during early colonization. Oxidative stress defense and stringent response genes were found to be maximally induced during the acute infectious phase. Overall, these findings reveal possible mechanisms involved in adaptation of <italic>Campylobacter</italic> to the host.</p>