AUTHOR=Knapp Angela TITLE=The sensitivity of marine N2 fixation to dissolved inorganic nitrogen JOURNAL=Frontiers in Microbiology VOLUME=3 YEAR=2012 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2012.00374 DOI=10.3389/fmicb.2012.00374 ISSN=1664-302X ABSTRACT=

The dominant process adding nitrogen (N) to the ocean, di-nitrogen (N₂) fixation, is mediated by prokaryotes (diazotrophs) sensitive to a variety of environmental factors. In particular, it is often assumed that consequential rates of marine N₂ fixation do not occur where concentrations of nitrate (NO⁻₃) and/or ammonium (NH⁺₄) exceed 1μM because of the additional energetic cost associated with assimilating N₂ gas relative to NO⁻₃ or NH⁺₄. However, an examination of culturing studies and in situ N₂ fixation rate measurements from marine euphotic, mesopelagic, and benthic environments indicates that while elevated concentrations of NO⁻₃ and/or NH⁺₄ can depress N₂ fixation rates, the process can continue at substantial rates in the presence of as much as 30μM NO⁻₃ and/or 200μM NH⁺₄. These findings challenge expectations of the degree to which inorganic N inhibits this process. The high rates of N₂ fixation measured in some benthic environments suggest that certain benthic diazotrophs may be less sensitive to prolonged exposure to NO⁻₃ and/or NH⁺₄ than cyanobacterial diazotrophs. Additionally, recent work indicates that cyanobacterial diazotrophs may have mechanisms for mitigating NO⁻₃ inhibition of N₂ fixation. In particular, it has been recently shown that increasing phosphorus (P) availability increases diazotroph abundance, thus compensating for lower per-cell rates of N₂ fixation that result from NO⁻₃ inhibition. Consequently, low ambient surface ocean N:P ratios such as those generated by the increasing rates of N loss thought to occur during the last glacial to interglacial transition may create conditions favorable for N₂ fixation and thus help to stabilize the marine N inventory on relevant time scales. These findings suggest that restricting measurements of marine N₂ fixation to oligotrophic surface waters may underestimate global rates of this process and contribute to uncertainties in the marine N budget.