%A Iannelli,Francesco %A Santagati,Maria %A Santoro,Francesco %A Oggioni,Marco %A Stefani,Stefania %A Pozzi,Gianni %D 2014 %J Frontiers in Microbiology %C %F %G English %K Tn1207.3,Streptococcus pyogenes,macrolide resistance,Tn1207.1,Ice,Φ1207.3,Prophages %Q %R 10.3389/fmicb.2014.00687 %W %L %M %P %7 %8 2014-December-09 %9 Original Research %+ Prof Gianni Pozzi,University of Siena,Department of Medical Biotechnologies,Siena,53100,Italy,gianni.pozzi@unisi.it %# %! Φ1207.3 of Streptococcus pyogenes %* %< %T Nucleotide Sequence of Conjugative Prophage Φ1207.3 (formerly Tn1207.3) carrying the mef(A)/msr(D) genes for efflux resistance to macrolides in Streptococcus pyogenes %U https://www.frontiersin.org/articles/10.3389/fmicb.2014.00687 %V 5 %0 JOURNAL ARTICLE %@ 1664-302X %X Genetic element Φ1207.3 (formerly Tn1207.3) is a prophage of Streptococcus pyogenes which carries the macrolide efflux resistance genes mef(A)/msr(D) and is capable of conjugal transfer among streptococci. Complete nucleotide sequence showed that Φ1207.3 is 52,491 bp in length and contained 58 open reading frames (ORFs). A manual homology-based annotation with functional prediction of the hypothetical gene product was possible only for 34 out of 58 ORFs. Φ1207.3 codes for two different C-methylation systems, several phage structural genes, a lysis cassette (composed by a holin and a peptidoglycan hydrolase), and three site-specific resolvases of the serine recombinase family.