Redox regulation by reversible protein S-thiolation in bacteria

Low molecular weight (LMW) thiols function as thiol-redox buffers to maintain the reduced state of the cytoplasm. The best studied LMW thiol is the tripeptide glutathione (GSH) present in all eukaryotes and Gram-negative bacteria. Firmicutes bacteria, including Bacillus and Staphylococcus species utilize the redox buffer bacillithiol (BSH) while Actinomycetes produce the related redox buffer mycothiol (MSH). In eukaryotes, proteins are post-translationally modified to S-glutathionylated proteins under conditions of oxidative stress. S-glutathionylation has emerged as major redox-regulatory mechanism in eukaryotes and protects active site cysteine residues against overoxidation to sulfonic acids. First studies identified S-glutathionylated proteins also in Gram-negative bacteria. Advances in mass spectrometry have further facilitated the identification of protein S-bacillithiolations and S-mycothiolation as BSH- and MSH-mixed protein disulfides formed under oxidative stress in Firmicutes and Actinomycetes, respectively. In Bacillus subtilis, protein S-bacillithiolation controls the activities of the redox-sensing OhrR repressor and the methionine synthase MetE in vivo. In Corynebacterium glutamicum, protein S-mycothiolation was more widespread and affected the functions of the maltodextrin phosphorylase MalP and thiol peroxidase (Tpx). In addition, novel bacilliredoxins (Brx) and mycoredoxins (Mrx1) were shown to function similar to glutaredoxins in the reduction of BSH- and MSH-mixed protein disulfides. Here we review the current knowledge about the functions of the bacterial thiol-redox buffers glutathione, bacillithiol, and mycothiol and the role of protein S-thiolation in redox regulation and thiol protection in model and pathogenic bacteria.


Introduction
The cytoplasm is a reducing environment and protein thiols are maintained in their reduced state by low molecular weight (LMW) thiol-redox buffers and enzymatic thiol-disulfide oxidoreductases, including the thioredoxin and glutaredoxin systems (Fahey, 2013;Van Laer et al., 2013). In their natural environment or during infections, bacteria encounter different reactive species, such as reactive oxygen, nitrogen, chlorine, and electrophilic species (ROS, RNS, RCS, RES) (Antelmann and Helmann, 2011;Gray et al., 2013a). These reactive species cause different post-translational thiol-modifications in proteins and activate or inactivate specific transcription factors resulting in expression of detoxification pathways. LMW thiol-redox buffers function in detoxification of different reactive species and are often present in millimolar concentrations in the cytoplasm.
The best studied LMW thiol is glutathione (GSH) present in eukaryotes and Gram-negative bacteria (Fahey, 2013). Most Gram-positive bacteria do not produce GSH. Instead, the Actinomycetes utilize mycothiol (MSH) as thiol-redox buffer (Jothivasan and Hamilton, 2008;. In Bacillus megaterium, Bacillus cereus, and Staphylococcus aureus, coenzyme A (CoASH) serves as an abundant LMW thiol . Many Firmicutes bacteria, including Bacillus and Staphylococcus species utilize bacillithiol (BSH) and cysteine as major thiol-redox buffers (Newton et al., 2009). Alternative LMW thiols include also the betaine-histidine derivative ergothioneine that compensates for the absence of MSH in Mycobacterium smegmatis mshA mutants (Ta et al., 2011). Cysteine is used for alternative S-thiolations in the absence of BSH and MSH in Bacillus subtilis and Corynebacterium glutamicum since S-cysteinylated proteins were identified in bsh and msh mutants (Chi et al., 2011. The protozoa Leishmania and Trypanosoma produce the glutathione-derivative trypanothione (bis-glutathionylspermidine or TSH 2 ). In Escherichia coli, glutathionylspermidine (GSP) was detected during the stationary phase (Fahey, 2013). Some microaerophilic γ-proteobacteria utilize glutathione amide (GASH) which forms a persulfide (GASSH) during photoautotrophic growth on high concentrations of sulfide (Bartsch et al., 1996).
Under conditions of oxidative stress, LMW thiols form mixed disulfides with protein thiols which is termed protein S-thiolation. In eukaryotes, protein S-glutathionylation has emerged as major redox-regulatory mechanism that controls the activity of redox sensing transcription factors and protects active site Cys residues against irreversible oxidation to sulfonic acids (Dalle-Donne et al., 2009). S-glutathionylation controls numerous physiological processes, such as cellular growth and differentiation, cell cycle progression, transcriptional activity, cytoskeletal function, cellular metabolism, and apoptosis (Klatt and Lamas, 2000;Ghezzi, 2005Ghezzi, , 2013Dalle-Donne et al., 2007). S-glutathionylation must meet several criteria to function as redox-control mechanism: (1) reversibility, (2) specificity to active site Cys, (3) change in protein function/activity, and (4) induction by ROS or RNS. S-glutathionylation serves as a form of GSH storage to prevent the export of GSSG under oxidative stress (Dalle-Donne et al., 2009). Many eukaryotic proteins, like α-ketoglutarate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase, ornithine δ-aminotransferase, pyruvate kinase, heat specific chaperones, and regulatory proteins (c-Jun, NF-κB) are reversibly inactivated or activated by S-glutathionylation (Dalle-Donne et al., 2009;Kehr et al., 2011). However, the regulatory role of protein S-thiolation for bacterial physiology has only recently been investigated. Here we review the current knowledge about the functions of the bacterial redox buffers GSH, MSH, and BSH and their roles for protein S-thiolations in GSH-, MSH-and BSH-producing bacteria.
During the oxidative burst, activated neutrophils release O 2 • − , H 2 O 2 , nitric oxide (NO), and hypochlorous acid (HOCl) with the aim to kill invading pathogenic bacteria (Forman and Torres, 2001;Winterbourn and Kettle, 2013). The neutrophil NADPH oxidase (NOX) shuttles electrons from NADPH to O 2 in the phagosomal lumen and generates around 20 µM superoxide anion. Myeloperoxidase (MPO) is released upon degranulation in millimolar concentrations. MPO catalyzes the dismutation of O 2 • − to H 2 O 2 and subsequent conversion of H 2 O 2 and chloride to HOCl (Figure 1) (Winterbourn and Kettle, 2013). NO is generated in neutrophils by the inducible nitric oxide synthase (iNOS) catalyzing the oxidation of L-arginine to L-citrulline. The reaction of NO with O 2 • − leads to formation of peroxynitrite (ONOO − ). Thus, neutrophils release ROS, RNS, and the highly reactive HOCl as antimicrobial defense mechanism.
Reactive electrophilic species (RES) have electron-deficient centers and can react with the nucleophilic Cys thiol group via the thiol-S-alkylation chemistry (Figure 2) (Antelmann and Helmann, 2011). RES include quinones, aldehydes, epoxides, diamide and α,β-unsaturated dicarbonyl compounds. RES are often generated as secondary reactive intermediates from oxidation products of amino acids, lipids or carbohydrates (Marnett et al., 2003;Rudolph and Freeman, 2009). Quinones are lipid-electron carriers of the respiratory chain, including FIGURE 1 | Generation of Reactive Oxygen Species (ROS) during respiration and HOCl production by activated neutrophils during infections. ROS are generated in bacteria during respiration by stepwise one-electron transfer to O 2 producing superoxide anion, hydrogen peroxide and hydroxyl radical. The highly reactive hydroxyl radical is also produced from H 2 O 2 and Fe 2+ in the Fenton reaction. During infections, activated neutrophils generate superoxide anion by the NADPH oxidase (NOX) that is converted to H 2 O 2 by the superoxide dismutase (SOD). Myeloperoxidase (MPO) is released upon degranulocytosis producing the highly reactive hypochlorous acids (HOCl) from H 2 O 2 and Cl − as potent killing agent for pathogenic bacteria. In quinones and aldehydes the electrons are drawn to carbonyl oxygen leaving the partial positive charges at neighboring carbon atoms that become electrophilic. Diamide is an electrophilic azocompound that causes disulfide stress. (B) Quinones can react as electrophiles with the nucleophilic thiol group of Cys residues via thiol-S-alkylation leading to irreversible thiol-S-adduct formation. In the oxidative mode, quinones are incompletely reduced to semiquinone radicals that generate superoxide anions and can oxidize protein thiols to disulfides.
ubiquinone and menaquinone (Farrand and Taber, 1974). Soil bacteria encounter quinones as redox active components of humic substances and in dissolved organic matter (Ratasuk and Nanny, 2007). The toxic dicarbonyl compound methylglyoxal is produced in all organisms from triose-phosphate intermediates as byproduct of the glycolysis and can be generated also from amino acids metabolism (Ferguson et al., 1998;Booth et al., 2003;Kalapos, 2008). Bacteria also have to cope with the carbonyl compound formaldehyde. Formaldehyde is an intermediate in the C 1 -metabolism of methanotrophic and methylotrophic bacteria and is ubiquitously distributed in the environment. Thus, bacteria have evolved conserved pathways for detoxification of formaldehyde and methylglyoxal that involve LMW thiols.
In eukaryotic cells, RES are implicated in many pathophysiological processes and modulate signaling pathways (Mackay and Knock, 2015). Eukaryotic cells produce lipid-derived RES, such as malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE) (Rudolph and Freeman, 2009). HNE is generated from polyunsaturated fatty acids in biological membranes by a radicalbased peroxidation chain reaction (Jacobs and Marnett, 2010). Furthermore, 15-deoxy-12,14-prostaglandin J2 (15d-PGJ2) is generated from arachidonic acid during inflammation and 2-trans-hexadecenal (2-HD) is produced during sphingolipid metabolism which promotes apoptosis . Bacterial membrane lipids also contain unsaturated fatty acids which are synthesized at higher levels during adaptation to cold shock to maintain the fluidity of the membrane (De Mendoza, 2014). These unsaturated fatty acids in bacterial membrane lipids could be the target for ROS leading to lipid peroxidation products in bacteria. Lipid hydroperoxides, such as linoleic acid hydroperoxide are sensed by the redox-sensing MarR-type repressor OhrR.
OhrR regulates the peroxiredoxin OhrA that functions in detoxification of organic hydroperoxides Fuangthong et al., 2001). However, the fatty acid-derived peroxidation product which is sensed by OhrR in vivo remains to be identified.
Post-Translational Thiol-Modifications of Proteins by ROS, RES, and RCS in Bacteria ROS, RES, and RCS can damage all cellular macromolecules including proteins, nucleic acids or carbohydrates (Imlay, 2008(Imlay, , 2013Jacobs and Marnett, 2010;Gray et al., 2013a). However, in eukaryotes low levels of ROS and RES act also as second messengers to modulate signal transduction pathways (Rudolph and Freeman, 2009;Mackay and Knock, 2015). Bacterial transcription factors often use redox-sensitive Cys residues for sensing of ROS, RES, and RCS to control the expression of specific detoxification pathways (Antelmann and Helmann, 2011;Gray et al., 2013a). The thiol group of cysteine is subject to reversible and irreversible post-translational thiol-modifications that lead to inactivation or activation of the transcription factor. Protein thiols can be reversibly oxidized to protein disulfides and irreversibly overoxidized to sulfinic or sulfonic acids by ROS (Antelmann and Helmann, 2011). ROS lead first to oxidation of protein thiols to Cys sulfenic acids as unstable intermediates (R-SOH) (Figure 3). Cys sulfenic acid rapidly reacts further with other thiols to form intramolecular and intermolecular protein disulfides or mixed disulfides with LMW thiols, collectively termed as S-thiolations (e.g., S-cysteinylations, S-glutathionylations, Smycothiolations, and S-bacillithiolations). Protein S-thiolations protect the thiol groups against the irreversible overoxidation to FIGURE 3 | Thiol-chemistry of ROS and HOCl with thiol-containing proteins. The Cys thiol group is oxidized by ROS to an unstable Cys sulfenic acid intermediate (Cys-SOH) that reacts further with proximal thiols to form intramolecular and intermolecular disulfides or mixed disulfides with LMW thiols (RSH), such as glutathione, bacillithiol or cysteine, termed as S-thiolations. HOCl leads to chlorination of protein thiols to sulfenylchloride intermediates (Cys-SCl) that react further to form disulfides. In the absence of proximal thiols, the chlorinated Cys is overoxidized to Cys sulfinic and sulfonic acids. Disulfides function as redox switches to control protein activity and protect thiol groups against overoxidation to Cys sulfinic and sulfonic acids.
Cys sulfinic (R-SO 2 H) and sulfonic acid (R-SO 3 H). This is particularly important for essential and abundant proteins whose overoxidation would lead to loss of cell viability and requires new protein synthesis to replace inactivated proteins. However, eukaryotic sulfiredoxins are able to reduce sulfinic acids in 2-Cys peroxiredoxins, but sulfiredoxins are not present in bacteria (Lowther and Haynes, 2011).
Hypochloric acid (HOCl) is a strong two-electron oxidant and chlorinating agent with a high redox potential [E 0 ′ (HOCl/Cl − ) = 1.28 mV] (Davies, 2011). HOCl targets most strongly the sulfurcontaining amino acids cysteine and methionine with the second-order rate constant of k = 3 × 10 7 M −1 s −1 (Hawkins et al., 2003). HOCl first chlorinates the thiol group to form the unstable sulfenylchloride intermediate that reacts further with another thiol group to disulfides. In the absence of another thiol, the chlorinated thiol group is overoxidized very rapidly to sulfinic or sulfonic acids (Hawkins et al., 2003) (Figure 3). We confirmed that strong disulfide stress responses are caused by HOCl in different Gram-positive bacteria in vivo and detected mixed protein disulfides with Cys, BSH, and MSH as major oxidation products (Chi et al., 2011(Chi et al., , 2013. RNS cause reversible thiol-modifications: nitric oxide (NO) leads to S-nitrosothiol formation (RS-NO) and peroxinitrite (ONOO − ) causes S-nitrothiol (RS-NO 2 ) formation. Alternatively, S-nitrosothiol (e.g., GSNO or MSNO) can be formed by direct reaction of NO with LMW thiols (Antelmann and Helmann, 2011).
RES can react via the thiol-S-alkylation chemistry with Cys thiols. However, quinones have two modes of action, an oxidation and an alkylation mode. In the oxidation mode, the one-electron reduction of quinones generates the highly reactive semiquinone radical leading to generation of superoxide anions (Figure 2). The electrophilic reaction of quinones involves the 1,4-reductive Michael-type addition of thiols to quinones (Marnett et al., 2003). Quinones lead to irreversible thiol-S-alkylation and protein aggregation to deplete protein thiols in the proteome in vivo (Liebeke et al., 2008). However, non-toxic quinone concentrations resulted in reversible thiol-disulfide switches in RES-sensing redox regulators (e.g., YodB, CatR, QsrR, NemR) to activate the expression of specific quinone detoxification pathways (Antelmann and Helmann, 2011;Gray et al., 2013a;Lee et al., 2013). Methylglyoxal reacts with nucleophilic centers of the DNA and with the amino acids arginine, lysine and cysteine causing advanced glycation end-products (Bourajjaj et al., 2003). The lipid-derived electrophiles MDA and HNE were shown to alkylate DNA bases and protein thiols leading to DNA and membrane damages in eukaryotes (Rudolph and Freeman, 2009).

Biosynthesis and Functions of Major LMW Thiol-Redox Buffers in Bacteria
Biosynthesis, Uptake, and Functions of Glutathione in Bacteria The tripeptide glutathione (γ-glutamylcysteinyl-glycine; GSH) is utilized as major LMW thiol-redox buffer in Gram-negative bacteria and in some Gram-positive Firmicutes bacteria, including Streptococcus agalactiae, Listeria monocytogenes, and Clostridium acetobutylicum (Figure 4). In E. coli, GSH biosynthesis occurs in two steps: The γ-glutamate cysteine ligase (GshA) catalyzes the formation of γ-glutamylcysteine (γ-Glu-Cys) from glutamate and cysteine. In the second step, ligation of glycine to γ-Glu-Cys is catalyzed by glutathione synthase (GshB) (Meister, 1995;Anderson, 1998). In S. agalactiae and L. monocytogenes, a bifunctional fusion protein GshF is present that exhibits both GshA and GshB activities (Gopal et al., 2005;Janowiak and Griffith, 2005). Interestingly, Lactococcus lactis, Streptococcus pneumoniae and Haemophilus influenzae do not synthesize GSH, but encode GSH-uptake mechanisms. In S. pneumoniae, the GSH-uptake from the host is mediated by the ABC transporter binding protein GshT (Potter et al., 2012;Vergauwen et al., 2013). In addition, the cystine importer TcyBC was shown to be primed for GSH import by GshT. In H. influenzae, GSH import is mediated by the ABC-transporter DppBCDF and requires the periplasmic GSHbinding protein GbpA (Vergauwen et al., 2010). Strikingly, these pathogens utilize host-derived GSH as protection mechanism against the host immune defense.
GSH is present in millimolar concentrations in the cytoplasm of E. coli (Masip et al., 2006;Fahey, 2013). GSH maintains protein thiols in the reduced state and serves as a storage form of cysteine. In contrast to cysteine, GSH is resistant to metal-catalyzed autooxidation because of its bound Cys amino and carboxyl groups that prevent ligation of heavy metal ions (Fahey, 2013). During the bacterial growth and under oxidative stress, GSH is oxidized to glutathione disulfide (GSSG). The NADPH-dependent glutathione reductase (Gor) keeps GSH in its reduced state to maintain a high GSH/GSSG ratio ranging from 30:1 to 100:1 in the cytoplasm. The standard thiol-disulfide redox potential of the GSH redox couple was calculated as E 0′ (GSSG/GSH) = −240 mV at physiological pH values (Hwang et al., 1995;Van Laer et al., 2013).
The various detoxification functions of GSH have been extensively studied in E. coli gsh mutants. In E. coli, GSH functions in detoxification of ROS, RES, RCS, RNS, xenobiotics, antibiotics, toxic metals, and metalloids (Masip et al., 2006) (Table 1). Detoxification of xenobiotics, electrophiles and antibiotics by GSH occurs either spontaneously by S-conjugation or by the catalytic activity of GSH-S-transferases (Fahey, 2013). The GSH-S-conjugates are usually excreted from the cell as non-toxic mercapturic acid derivatives. GSH was shown to function as a cofactor in methylglyoxal detoxification in E. coli. The major pathway for methylglyoxal detoxification in E. coli is the GSHdependent glyoxalase pathway. The glyoxalase-I (GloA) catalyzes formation of S-lactoyl-GSH from GSH-hemithioacetal and glyoxalase-II (GloB) converts S-lactoyl-GSH to D-lactate (Ferguson et al., 1998;Booth et al., 2003). In addition, glyoxalase-III operates GSH-independently to convert methylglyoxal to lactate. The glyoxalase-I encoding gloA gene and the nemRA operon are redox-regulated by the NemR repressor under methylglyoxal, quinone and HOCl stress (Gray et al., 2013b;Lee et al., 2013;Ozyamak et al., 2013). The glyoxalase GloA and the oxidoreductase NemA are important for methylglyoxal survival and confer resistance to methylglyoxal in E. coli (Ozyamak et al., 2013). The resistance to methylglyoxal is also linked to the activation of potassium efflux by the S-lactoyl-GSH intermediate leading to cytoplasmic acidification (Ferguson et al., 1998;Booth et al., 2003). The cytoplasmic acidification limits the interaction of methylglyoxal with DNA bases. Thus, potassium efflux and detoxification by GloA protect against methylglyoxal toxicity in E. coli.
Interestingly, expression of the E. coli gshAB genes in the industrial important Clostridium acetobutylicum enhances robustness and alcohol production as a promising strategy for engineering industrial production strains. Thus, GSH protects also against large-scale ethanol and butanol production in C. acetobutylicum during fermentation (Hou et al., 2013).

Functions of Glutathione in the Virulence of Pathogenic Bacteria
GSH has many detoxification functions to maintain the redox balance of the cytoplasm, but only recently the role of GSH for the control of virulence functions has been explored in the pathogenic bacteria S. pneumoniae, L. monocytogenes, and Salmonella Typhimurium (Potter et al., 2012;Song et al., 2013;Reniere et al., 2015) ( Table 2). In S. pneumoniae, the glutathione reductase Gor and the GSH-importer GshT were required for oxidative stress protection and metal ion resistance. Moreover, the gshT mutant was attenuated in colonization and invasion in a mouse model of pneumococcal infection (Potter et al., 2012). Thus, GSH protects against the host immune defense and contributes to fitness of S. pneumoniae. The intracellular pathogen L. monocytogenes is able to synthesize GSH via the gshF fusion protein, but GSH can be also imported from the host (Reniere et al., 2015). The L. monocytogenes gshF mutant was two-fold less virulent compared to the wild type in a mice model. In addition, the gshF mutant was sensitive to oxidative stress, contains lower levels of ActA and formed small plaques in tissue culture assays that measure cellto-cell spread (Reniere et al., 2015). The Actin assembly-inducing protein ActA is controlled by the virulence regulator PrfA and used by L. monocytogenes to move through the host cells (Freitag et al., 2009). It was shown that the virulence phenotype of the gshF mutant is caused by the lack of PrfA activation by bacterial and host-derived GSH (Reniere et al., 2015). Interestingly, activation of PrfA is mediated by an allosteric binding of GSH to PrfA, but not by S-glutathionylation. Thus, GSH plays a role as signaling molecule to activate virulence gene expression in an intracellular pathogen.
In S. Typhimurium, GSH antagonizes the bacteriostatic effects of RNS in vivo and gshA mutants were sensitive to ROS and RNS (Song et al., 2013). Thus, GSH presents a first line defense against ROS and RNS produced by the host immune system. This was shown in an acute model of salmonellosis in mice expressing the wild-type NRAMP1 R allele (natural resistance-associated macrophage protein 1) which is linked to high NO production of the macrophages. The gshA and gshB mutants were attenuated in this acute model of salmonellosis. It was further shown that GSH protects against ROS and RNS produced by the NADPH phagocyte oxidase and inducible nitric oxide synthase (iNOS) in this mice model (Song et al., 2013). These recent studies highlight the important roles of GSH in the control of virulence functions, expression of virulence factors and pathogen fitness under infection conditions in S. pneumoniae, L. monocytogenes, and

S-Glutathionylation at a Global Scale
Advances in probe design and mass spectrometry-based thioltrapping approaches have facilitated the detection of specific reversible thiol-modifications, including sulfenylation, nitrosylation, glutathionylation, and sulfhydrations of proteins at a global scale (Leonard and Carroll, 2011;Pan and Carroll, 2013;Paulsen and Carroll, 2013;Gupta and Carroll, 2014;Zhang et al., 2014). Different methods have been applied for specific detection of S-glutathionylations in eukaryotic cells, including the use of GSH-specific antibodies and the labeling of S-thiolations with 35 S-cysteine followed by 2D gel electrophoresis and phosphoimaging (Fratelli et al., 2002(Fratelli et al., , 2003(Fratelli et al., , 2004. However, the specificity of the GSH antibodies is questionable and the gelbased detection of S-thiolations using 35 S-cysteine does not distinguish between S-cysteinylations and S-glutathionylations or other forms of S-thiolations. Hence, more sensitive mass spectrometry-based redox proteomics methods have been developed, including the glutaredoxin-coupled NEM-biotin switch assay and the treatment of cell extracts with N,N-biotinyl glutathione disulfide (BioGSSG) (Lind et al., 2002;Brennan et al., 2006;Kehr et al., 2011;Zaffagnini et al., 2012a) (Figure 5).
Both methods make use of the specific streptavidin enrichment of biotinylated peptides that improve the identification of S-glutathionylated peptides using mass spectrometry. The NEM biotin-switch assay was successfully applied to detect protein S-glutathionylation in eukaryotic endothelial cells and malaria parasites which applies glutaredoxin for reduction of protein-SSG followed by NEM-biotin alkylation and enrichment using streptavidin columns (Lind et al., 2002;Kehr et al., 2011). The biotin-GSSG approach has been applied to identify S-glutathionylated proteins in the green algae Chlamydomonas reinhardtii (Zaffagnini et al., 2012a) and in the photosynthetic cyanobacterium Synechocystis sp. PCC6803 (Chardonnet et al., 2015). In total, 383 S-glutathionylated proteins were identified in Synechocystis sp. PCC6803 and 125 glutathionylation sites were mapped by mass spectrometry. In addition, the peroxiredoxin PrxII (Sll1621) and the 3-phosphoglycerate dehydrogenase PGDH (Sll1908) could be S-glutathionylated by BioGSSG in vitro (Chardonnet et al., 2015). In another approach, biotinyl-spermine (biotine-spm) and in vivo expressed E. coli GspS have been applied for mammalian cells to convert GSH to biotin-glutathionylspermidine (biotin-Gsp) which subsequently modified proteins by Biotin-Gsp-Sthiolation (Chiang et al., 2012;Lin et al., 2015). The biotine-spm is removed enzymatically by GspA from the enriched biotin-Gsp-S-thiolated peptides and the GSS-peptides are identified by mass spectrometry. This approach allows the identification of Sglutathionylation sites without the biotin-tag and enhances the coverage for S-glutathionylated proteins. In mammalian cells, 1409 S-glutathionylated cysteines in 913 proteins were identified using the Gsp-biotin approach (Chiang et al., 2012;Lin et al., 2015). This makes the application of this chemoenzymatic approach using the GspS enzyme attractive for global and specific studies of S-glutathionylated proteins.
In S. Typhimurium, protein S-glutathionylation has been studied at a global scale by top-down and bottom-up proteomic approaches (Ansong et al., 2013). Top-down proteomics uses whole proteins for separation and fragmentation directly FIGURE 5 | Redox proteomics methods to study protein S-glutathionylation at a global scale. Mass spectrometry-based methods for identification of S-glutathionylations include the glutaredoxin-coupled NEM-biotin switch assay (A), the biotin-Gsp assay, (B) or the N,N-biotinyl glutathione disulfide (BioGSSG) assay, (C) (Lind et al., 2002;Brennan et al., 2006;Kehr et al., 2011;Zaffagnini et al., 2012a). In the biotin-Gsp assay, E.
in the mass spectrometer. In bottom-up proteomics approaches proteins are digested by a protease and the peptide mixtures are analyzed by mass spectrometry to identify proteins at the peptide level. The top-down proteomic approach identified 563 proteins with 1665 post-translational modifications in S. Typhimurium. The authors identify 25 S-thiolated proteins in cells grown in complete LB medium including 16 S-glutathionylated proteins and nine S-cysteinylated proteins. Interestingly, a subset of nine S-glutathionylated are modified by S-cysteinylation in infection-like minimal LPM medium (Table 3). This could indicate a shift from S-glutathionylation to S-cysteinylation under infection-like conditions in S. Typhimurium. These Sthiolated proteins include phosphoglycerate kinase (Pgk), elongation factor (Tuf) and enolase (Eno) that are also targets for S-glutathionylations in endothelial cells (Fratelli et al., 2002). The top-down proteomics results were verified by bottomup proteomics approaches to identify the specific S-thiolated Cys peptides (Ansong et al., 2013). Structural analysis revealed that S-glutathionylation occurred mostly at buried Cys residues and not at surface-exposed Cys. S-glutathionylation on buried Cys was also shown for the enolase whose activity is known to be modified by S-thiolation in human cells (Fratelli et al., 2002). It is postulated that S. Typhimurium switches from Sglutathionylation to S-cysteinylation during infection conditions as novel redox-control mechanism. In agreement with the proteome data, transcriptome results point to an up-regulation of Cys biosynthesis and down-regulation of GSH biosynthesis under infection-like conditions. However, the physiological role of this S-thiolation switch for redox control of the identified protein targets under ROS stress remains to be elucidated.
Furthermore, no blocking of reduced thiols with NEM or IAM was performed to avoid artificial disulfide formation. Thus, it remains to be verified that the observed S-thiolations are not caused by artificial thiol-disulfide exchange. Previous studies have also shown that L. monocytogenes is able to both import and synthesize GSH (Reniere et al., 2015). Furthermore, the non-GSH-utilizing S. aureus was shown to import GSH during growth in LB medium (Pöther et al., 2013). Thus, the possible uptake of GSH in S. Typhimurium from LB-medium could contribute to the observed S-glutathionylations which needs to be investigated.

The Regulatory Potential of Protein S-Glutathionylation in Gram-negative Bacteria
The role of protein S-glutathionylation for redox control has been studied in few Gram-negative bacteria, including E. coli, S. Typhimurium, Neisseria meningitidis, Pseudoalteromonas haloplanktis, and Synechocystis sp. PCC6803 (Table 3). In E. coli, the peroxide-sensing regulator OxyR is activated by Sglutathionylation at its redox-sensing Cys199 in vitro (Kim et al., 2002). In addition, the activities of glyceraldehyde-3-phosphate dehydrogenase, methionine synthase and the PAPS reductase are inhibited by S-glutathionylation in E. coli (Lillig et al., 2003;Hondorp and Matthews, 2004;Brandes et al., 2009). A recent study provides a model for the S-glutathionylation of the conserved active site Cys in GapDH and explains the reactivity of the active site toward H 2 O 2 (Peralta et al., 2015). Reaction of the active site Cys with H 2 O 2 is catalyzed by a mechanism which stabilizes the transition state and promotes leaving group departure by providing a proton relay. This model suggests the conserved  redox-regulation of GapDH by S-thiolation of its active site Cys across all domains of life.
In Neisseria meningitidis, an esterase EstD acts together with the GSH-dependent alcohol dehydrogenase AdhC in formaldehyde detoxification. EstD is inactivated via S-glutathionylation at its conserved Cys54 by its substrate S-formyl-GSH during formaldehyde detoxification in vitro . In the psychrophilic bacterium Pseudoalteromonas haloplanktis, Sglutathionylation of the iron-superoxide dismutase PhSOD at the single Cys57 protected the enzyme from tyrosine nitration and peroxynitrite inactivation in vitro and in vivo (Castellano et al., 2008).
In Synechocystis sp. PCC6803, a MerA-like enzyme that functions in mercury and uranium reduction was shown to be redoxcontrolled by S-glutathionylation (Marteyn et al., 2013). MerA was S-glutathionylated at Cys78 that is required for mercury reduction resulting in inhibition of MerA activity. MerA redox regulation and reactivation required reduction by glutaredoxin-1 (Grx1). The active site Cys31 and Cys86 of Grx-1 operate in MerA interactions and both Cys are required for MerA reactivation. Furthermore, S-glutathionylation was shown to control the activity of the transcription factor AbrB2 in Synechocystis sp. PCC6803 (Cassier-Chauvat et al., 2014). AbrB2 is a repressor of the hydrogenase-encoding hoxEFUYH operon and also down-regulates antioxidant genes, such as cydAB encoding the cytochrome bd-quinol oxidase and norB encoding the nitric oxide reductase. The production of hydrogen is thought to be an antioxidant mechanism to eliminate electrons for oxygen reduction and ROS generation. AbrB2 contains a conserved single cysteine that is essential for redox-regulation and oligomerisation of AbrB2 as shown in C34S mutants. Sglutathionylation of Cys34 affected the binding of AbrB2 to the hox promoter and the stability of AbrB2 in vitro. In conclusion, S-glutathionylation has been shown to function in the redox-control of two transcriptional regulators, OxyR and AbrB2 in Gram-negative bacteria in vitro. However, compared to the many targets for S-glutathionylation that have been studied in eukaryotic organisms, there is much to be discovered about the regulatory potential of S-glutathionylation in bacteria.
The de-glutathionylation by Grx enzymes involves thioldisulfide exchange reactions with GSH via nucleophilic double displacement (ping-pong) mechanisms and occurs via mono-or di-thiol mechanisms. Most di-thiol Grx use monothiol mechanisms that take place in two steps: In the first step, the nucleophilic thiolate anion attacks the S-glutathionylated substrate protein, resulting in reduction of the mixed disulfide and the S-glutathionylated Grx (Grx-SSG) intermediate. This Grx-SSG intermediate is regenerated by GSH and Gor at expense of NADPH (Allen and Mieyal, 2012). The di-thiol mechanism involves a second active site Cys that forms an intramolecular disulfide to resolve the Grx-SSG intermediate that has been shown for some plant Grx enzymes (Zaffagnini et al., 2012b). However, this di-thiol mechanism of Grx is less efficient for protein de-glutathionylation and more involved in the reduction of intermolecular protein disulfides (Lillig et al., 2008;Allen and Mieyal, 2012). Thus far, the knowledge about Grx functions and substrates in most GSH-producing bacteria is scarce and remains an important subject for future studies.

Biosynthesis and Regulation of Bacillithiol in Gram-Positive Firmicutes Bacteria
Bacillithiol (BSH) is composed of Cys-GlcN-malate and serves as major LMW thiol in many Firmicutes bacteria, including Bacillus and Staphylococcus species, Deinococcus radiodurans, and Streptococcus agalactiae (Newton et al., 2009) (Figure 4). The BSH biosynthesis pathway was first identified in B. subtilis. In the first step, the glycosyltransferase BshA couples UDP-GlcNAc to L-malic acid for generation GlcNAc-Mal (Ruane et al., 2008;Gaballa et al., 2010;Parsonage et al., 2010). The deacetylase BshB1 catalyzes deacetylation of GlcNAc-Mal to GlcN-Mal. The last step involves the putative cysteine ligase YllA (BshC) that presumably adds Cys to GlcN-Mal (Gaballa et al., 2010). BshB1 has a paralog BshB2 and both enzymes have deacetylase activity. The functional redundancy of BshB1 and BshB2 in B. subtilis suggests that BshB2 might function as BSH-S-conjugate amidase Bca in detoxification of RES similar to the MSH-S-conjugate amidase Mca . The functions of the BshB1/2 homologs of B. anthracis (BA1557 and BA3888) and B. cereus (BC1534 and BC3461) in the deacetylation of GlcNAc-Mal have been demonstrated in vitro. In addition, BA3888 was shown to function as BSH-S-conjugate amidase (Bca) (Fang et al., 2013). In contrast to BshA and BshB, the activity of the putative cysteine ligase BshC has never been demonstrated biochemically in vitro. The structure of BshC was resolved revealing a core Rossmann fold with connecting peptide motifs (CP1 and CP2) and an αhelical coiled-coil domain required for dimerization (Vanduinen et al., 2015). BshC was crystallized with citrate and glycerol in the canonical active site and ADP bound in a second binding pocket that is different from the ADP-binding pocket in the related MshC structure. The active sites are solvent exposed and open for possible interactions with a protein, substrate or cofactor that remain to be elucidated to understand the catalytic mechanism of BshC (Vanduinen et al., 2015).
The regulation of the BSH biosynthesis genes has been studied in B. subtilis. The bshA and bshB1 genes belong to a large operon of seven genes including mgsA which encodes a methylglyoxal synthase. The bshB2 and bshC genes are encoded by two different operons. The bshA, bshB, and bshC genes are induced under conditions of disulfide stress provoked by diamide or NaOCl and positively controlled by the disulfide stress regulator Spx (Chi et al., 2011;Rochat et al., 2012;Gaballa et al., 2013). Consistent with the Spx-dependent control of the BSH biosynthesis genes, lower BSH levels were detected in the spx mutant using thiolmetabolomics (Chi et al., 2011;Rochat et al., 2012;Gaballa et al., 2013). It is interesting to note, that the Trx pathway and BSH biosynthesis genes are both regulated by the major disulfide stress regulator Spx in B. subtilis (Zuber, 2004(Zuber, , 2009Chi et al., 2011;Rochat et al., 2012;Gaballa et al., 2013).

Functions of Bacillithiol and BSH-Dependent Detoxification Enzymes
BSH is predominantly present in its reduced form in the cytoplasm with BSH/BSSB ratios ranging from 100:1 to 400:1 in B. subtilis indicating the presence of an efficient bacillithiol disulfide reductase . The FAD-dependent pyridine nucleotide disulfide oxidoreductase YpdA (IPR023856) was suggested to function as BSSB reductase because of its phylogenetic relationship to the BSH biosynthesis enzymes as revealed by a STRING search (Gaballa et al., 2010). However, the function of YpdA has not yet been demonstrated.
The standard thiol-redox potential of BSH was calculated as E 0 ′ (BSSB/BSH) = −221 mV which is higher than the GSH redox potential [E 0 ′ (GSSG/GSH) = −240 mV] . The microscopic pK a values of the thiol group of BSH were determined as pK a = 7.97 when the amino group of the Cys is protonated and as pK a = 9.55 in the presence of the deprotonated amino group of Cys . Thus, the thiol group in BSH is more acidic compared to the thiol group in Cys suggesting an enhanced level and reactivity of the BSH thiolate anions to detoxify reactive species. The BSH concentrations in B. subtilis vary during the growth in LB medium and increase strongly during the stationary phase to 3.5-5.2 mM. In contrast, the cellular Cys concentration is kept at a relatively low level (0.13-0.28 mM). Thus, BSH concentrations are ∼17-fold higher compared to the level of Cys . Similar concentrations of BSH (2 mM) were measured in Bacillus pumilus during growth. In B. pumilus, BSH levels increased under peroxide stress to 6 mM which is caused by an increased bshB expression (Handtke et al., 2014). BSH levels are also two-fold increased under diamide and NaOCl stress in B. subtilis due to Spx-dependent induction of bshA, bshB, and bshC (Chi et al., 2013;Gaballa et al., 2013). In S. aureus, the BSH levels are lower (0.3-1 mM) in the different clinical isolates (COL, USA300, Mu50, or N315) and BSH levels are not up-regulated during the stationary phase (Posada et al., 2014).
The physiological functions of BSH were studied in bsh mutants of B. subtilis and S. aureus ( Table 1). Phenotype analyses showed increased sensitivities of bsh mutants toward hypochlorite, diamide, methylglyoxal, ROS (paraquat, H 2 O 2 ), osmotic, and acidic stress, alkylating agents and fosfomycin in B. subtilis (Gaballa et al., 2010;Chi et al., 2011). The fosfomycin-sensitive phenotype of bsh mutants depends on the epoxide hydrolase FosB that requires BSH as a cofactor to open the ring structure for fosfomycin detoxification (Lamers et al., 2012;Roberts et al., 2013;Thompson et al., 2013) (Figure 6). FosB shows a preference for BSH as thiol cofactor and does only work poorly with Cys. The biochemical activity has been demonstrated for various Bacillus and Staphylococcus FosB homologs (Lamers et al., 2012;Roberts et al., 2013;Thompson et al., 2013). In B. subtilis and S. aureus, both FosB and BSH confer resistance to fosfomycin treatment in survival assays in vivo (Gaballa et al., 2010;Thompson et al., 2014). Co-crystallization of S. aureus FosB with L-Cys or BSH revealed a mixed disulfide at the active site Cys9 of FosB which is unique in FosB from S. aureus (Thompson et al., 2014).
Reactive electrophiles, such as monobromobimane are detoxified by direct conjugation to BSH or by conjugation reactions catalyzed by BSH S-transferases. BSH functions as a cofactor for DinB-family S-transferases that are widely distributed among GSH-, BSH-, and MSH-producing bacteria Perera et al., 2014). The B. subtilis DinB-family YfiT protein was active as S-transferase with BSH to conjugate monochlorobimane, but inactive with MSH or GSH . The yfiT gene is flanked by yfiS and yfiU encoding putative efflux transporters for mercapturic acids produced during electrophile detoxification. The YfiT-homolog of S. aureus BstA catalyzed the conjugation of BSH to monochlorobimane, 1chloro-2,4-dinitrobenzene and cerulenin, while rifampicin was BstA-independently conjugated to BSH (Perera et al., 2014).
BSH is involved in methylglyoxal detoxification and functions as a cofactor for BSH-dependent glyoxalases in B. subtilis . Methylglyoxal rapidly depletes BSH leading to BSH-hemithioacetal formation that is converted to S-lactoyl BSH by the glyoxalase-I (GlxA). The glyoxalase-II (GlxB) catalyzes conversion of S-lactoyl-BSH to lactate (Figure 6). Phenotype studies further indicated that BSH can detoxify heavy metal ions, such as tellurite and selenite in B. subtilis (Helmann, 2011). In addition, BSH functions as Zn buffer in metal ion homeostasis (Ma et al., 2014). The Cys thiol and carboxylate moieties of BSH can bind and store Zn(II) as BSH 2 :Zn complex under conditions of Zn(II) stress (Ma et al., 2014). BSH binding to Zn(II) occurred at much higher affinity compared to GSH. Mutants lacking BSH are more sensitive to Zn(II) stress and induced the Zn efflux CadA system at lower Zn levels compared to the wild type. BSH also protected against Zn(II) toxicity in cells lacking Zn efflux pumps. In addition, Zn efflux is elevated under conditions of diamide stress when the pool of reduced BSH is depleted. These results establish a new role of BSH as buffer for the labile Zn pool that are likely important for related pathogens under infection conditions.
In conclusion, functional analyses of bsh mutants established important roles of BSH as GSH surrogate in Firmicutes bacteria, including similar detoxification functions and BSH-dependent enzymes, such as DinB-family S-transferases and glyoxalases that are widely conserved across bacteria. However, the conserved role of FosB as BSH-dependent fosfomycin hydrolase and the function of BSH as Zn buffer have been described only in BSH-producing bacteria.

Functions of Bacillithiol in the Virulence of Staphylococcus aureus
Phenotype analyses of S. aureus bsh mutants were conducted for different clinical isolates of methicillin-resistant S. aureus strains (MRSA) that revealed a role of BSH for stress resistance and under infection conditions (Pöther et al., 2013;Posada et al., 2014) (Table 2). In survival assays, S. aureus USA300 LAC transposon bsh mutants were more sensitive to alkylating agents (iodoacetamide and CDNB), methylglyoxal, peroxide and superoxide stress, diamide, fosfomycin, cerulenin, rifamycin and metals ions, like copper and cadmium (Rajkarnikar et al., 2013). In S. aureus COL and USA300 backgrounds, bshA and fosB mutants with clean deletions showed increased sensitivities to fosfomycin, diamide and H 2 O 2 and the levels of NADPH and BSH were decreased in fosB mutants suggesting a function of FosB as S-transferase in the oxidative stress resistance (Posada et al., 2014). The S. aureus COL and USA300 bshA mutants showed a decreased survival in human whole-blood survival assays (Posada et al., 2014). Microarray analyses of the bshA mutant further revealed that staphyloxanthin biosynthetic genes are induced while the level of staphyloxanthin was strongly decreased in the S. aureus bshA mutant. Interestingly, the widely used strains of the S. aureus NCTC8325 lineage including SH1000 harbor natural yllA (bshC) null mutations that are caused by a 8 bp duplication in the bshC gene and these strains do not produce BSH (Gaballa et al., 2010;Newton et al., 2012;Posada et al., 2014). In contrast, S. aureus Newman encodes a functional bshC gene and produces BSH as revealed by thiol metabolomics (Newton et al., 2012;Pöther et al., 2013). BSH biosynthesis in S. aureus SH1000 could be restored by plasmid-encoded expression of the bshC gene (Pöther et al., 2013;Posada et al., 2014). In phagocytosis assays using murine macrophages and human epithelial cell lines the survival of the SH1000 strain was decreased compared to the bshC complemented S. aureus strain (Pöther et al., 2013;Posada et al., 2014). Thus, BSH is involved in the defense against the host-immune system and contributes to pathogen fitness in S. aureus clinical MRSA isolates under infection-related conditions. It will be exciting to unravel the regulatory mechanisms that contribute to virulence control by BSH in S. aureus.

The Role of Protein S-Bacillithiolation in Gram-Positive Firmicutes Bacteria
Protein S-bacillithiolation was recently discovered as a widespread thiol protection and redox-regulatory mechanism in different Firmicutes bacteria (Chi et al., 2011(Chi et al., , 2013 (Figure 7). S-bacillithiolation functions as a redox-switch mechanism to control the activity of redox-sensing transcription factors and metabolic enzymes, including OhrR and MetE (Lee et al., 2007;Chi et al., 2011) (Table 3). S-bacillithiolation of the OhrR repressor occurs at its lone Cys15 residue leading to inactivation of OhrR and expression of the thiol-dependent OhrA peroxiredoxin for detoxification of organic hydroperoxides and NaOCl (Fuangthong et al., 2001;Chi et al., 2011). S-bacillithiolation is also widespread among other Firmicutes with eight common and 29 unique S-bacillithiolated proteins identified in B. subtilis, Bacillus amyloliquefaciens, Bacillus pumilus, B. megaterium, and Staphylococcus carnosus (Chi et al., 2011(Chi et al., , 2013. The S-bacillithiolome contains mainly biosynthetic enzymes for amino acids (methionine, cysteine, branched chain and aromatic amino acids), cofactors (thiamine), nucleotides (GTP), as well as translation factors, chaperones, redox, and antioxidant proteins. Among the most conserved protein-SSB were abundant and essential proteins like TufA, MetE, GuaB that are targets for S-thiolation also in MSH-producing bacteria .
The methionine synthase MetE is the most abundant Sbacillithiolated protein in Bacillus species after NaOCl exposure. S-bacillithiolation of MetE occurs at its Zn-binding active site Cys730 and at the non-essential surface-exposed Cys719, leading to methionine starvation in NaOCl-treated cells (Chi et al., 2011). Similarly, methionine auxotrophy is caused by Sglutathionylation of MetE in E. coli after diamide stress (Hondorp and Matthews, 2004). The active site Zn center of MetE is also S-mycothiolated in C. glutamicum . Since formyl methionine is required for initiation of translation, MetE inactivation could stop translation during the time of hypochlorite detoxification. This translation arrest caused by S-bacillithiolation is supported by the strong repression of the  (Chi et al., 2011). S-bacillithiolation of OhrR inactivates the repressor and causes induction of the OhrA peroxiredoxin that confers NaOCl resistance. S-bacillithiolation of the methionine synthase MetE at its active site Cys730 and other enzymes of the Cys and Met biosynthesis pathway (YxjG, PpaC, SerA, MetI) leads to methionine auxotrophy (Chi et al., 2011(Chi et al., , 2013. In addition, other amino acids biosynthesis enzymes, translation factors and ribosomal proteins are S-bacillithiolated in Firmicutes bacteria. Thus, we hypothesize that S-bacillithiolation leads to a transient translation stop during the time of NaOCl detoxification to prevent further protein damage. NaOCl stress causes oxidation of BSH to BSSB and a two-fold decreased BSH/BSSB redox ratio that possibly contributes to S-bacillithiolation. The reduction of MetE-SSB and OhrR-SSB is catalyzed by bacilliredoxins (BrxA/B) in B. subtilis. stringent response RelA regulon under NaOCl stress, which includes genes for ribosomal proteins and translation factors (Chi et al., 2011).
Our studies revealed that S-bacillithiolations were observed under diamide and NaOCl stress, but not under control conditions. This confirms previous results about the mechanisms of S-glutathionylations which requires activation of protein thiols by ROS. S-glutathionylation can be caused via thiol-disulfide exchange with GSSG and by activation of thiols to sulfenic acid, sulfenylamides, thiyl radicals, thiosulfinate or S-nitrosyl intermediates (Gallogly and Mieyal, 2007;Mieyal et al., 2008;Allen and Mieyal, 2012;Mieyal and Chock, 2012). Hypochlorite leads to chlorination of the thiol group to form sulfenylchloride that is unstable and rapidly reacts further to form mixed BSH protein disulfides (Hawkins et al., 2003;Davies, 2011). The increased BSSB level under NaOCl-stress might also contribute to S-bacillithiolation via thiol-disulfide exchange.
Among the S-bacillithiolated proteins, the thioredoxin-like proteins YtxJ, YphP, and YqiW were identified in B. subtilis and Staphylococcus that occur only in BSH-producing bacteria (Chi et al., 2013). These Trx-like enzymes were suggested to function as bacilliredoxins (Brx) in the de-bacillithiolation process. YtxJ could functions as monothiol Brx and contains a single Cys in the conserved TCPIS motif. YphP (BrxA) and YqiW (BrxB) are paralogs of the uncharacterized DUF1094 family (53% identity) with unusual CGC active sites (Gaballa et al., 2010). YphP has also weak thiol-disulfide isomerase activity and a relatively high standard redox potential of E 0 ′ = −130 mV (Derewenda et al., 2009). It was demonstrated that BrxA and BrxB function in the reduction of the S-bacillithiolated substrates MetE and OhrR in vitro   (Figure 8). The BrxBCxA resolving Cys mutant protein was able to reduce S-bacillithiolated OhrR to restore the DNA-binding activity of OhrR. However, the BrxBCxA mutant was unable to reduce Scysteinylated OhrR. These results provide first evidence for the function of glutaredoxin-like enzymes in BSH-producing bacteria. However, phenotype analyses revealed that both, BrxA and BrxB are not essential and rather dispensable for oxidative stress resistance under conditions of S-bacillithiolations in B. subtilis . Thus, the bacilliredoxin pathway is redundant with other thiol-disulfide oxidoreductases or the thioredoxin pathway in vivo for reduction of BSH mixed disulfides. In conclusion, the redox regulation of enzymes and transcription regulators by S-bacillithiolation and bacilliredoxins has been studied in detail in the model bacterium B. subtilis. Future studies should be directed to elucidate if S-bacillithiolation and bacilliredoxins control virulence functions and pathogen fitness in the major pathogen S. aureus.

Biosynthesis and Regulation of Mycothiol in Actinomycetes
Mycothiol (MSH) is composed of N-Acetyl-Cys-GlcNmyoinositol (Figure 4) and is present in high-GC Gram-positive Actinomycetes, such as Streptomycetes, Mycobacteria and Corynebacteria (Jothivasan and Hamilton, 2008;. The biosynthesis of MSH proceeds from myo-inositol-1-phosphate, UDP-GlcNAc and cysteine and occurs in five steps (Jothivasan and Hamilton, 2008;. The glycosyltransferase MshA conjugates myo-inositol-1-P to UDP-GlcNAc and produces GlcNAc-Ins-P. Dephosphorylation of GlcNAc-Ins-P by the phosphatase MshA2 generates GlcNAc-Ins which is the substrate for the deacetylase MshB. The MshB enzyme is homologous to the MSH S-conjugate amidase (Mca), and has both deacetylase and amidase activities. The cysteine FIGURE 8 | Reduction of protein S-glutathionylations, S-bacillithiolations and S-mycothiolations by glutaredoxin, bacilliredoxin and mycoredoxin pathways. The S-glutathionylated proteins are reduced by glutaredoxins (Grx) leading to a Grx-SSG intermediate that is reduced by GSH leading to GSSG which is recycled back to GSH by the NADPH-dependent GSSG reductase (Gor). Analogous bacilliredoxin and mycoredoxin pathways have been characterized in BSH-and MSH-utilizing Gram-positive bacteria. The S-bacillithiolated proteins are reduced by bacilliredoxins (Brx) leading to Brx-SSB formation. Brx-SSB is reduced by BSH with the generation of BSSB that likely requires the NADPH-dependent BSSB reductase YpdA for regeneration of BSH. In Actinomycetes, mycoredoxin1 catalyzes reduction of S-mycothiolated proteins leading to Mrx1-SSM generation that is recycled by MSH and the NADPH-dependent MSSM reductase Mtr.
ligase MshC adds Cys to GlcN-Ins to generate Cys-GlcN-Ins. The final acetylation of the Cys is catalyzed by the acetyltransferase MshD to produce MSH (Jothivasan and Hamilton, 2008;. The structure of MSH is similar to that of BSH and the glycosyltransferase BshA and deacetylase BshB of B. subtilis are homologs of the MshA and MshB enzymes of Mycobacteria. MSH biosynthesis enzymes in Streptomycetes are redoxcontrolled under diamide stress by the disulfide stress specific σ R ECF sigma factor/RsrA anti sigma factor system . σ R is sequestered by its redox-sensitive anti sigma factor RsrA in non-stressed cells. RsrA is oxidized at redoxsensing Cys residues in the Zn-binding site under disulfide stress that leads to relief of σ R . Free σ R transcribes genes required to maintain the thiol-redox homeostasis, including the genes for TrxAB and MSH biosynthesis, such as mshA, mshB, mshC, mshD, mca (Bae et al., 2004;Park and Roe, 2008). In C. glutamicum, the homologous ECF sigma factor σ H /RshA system controls the disulfide stress response genes for the Trx/TrxR system (trxB, trxB1, trxC) and for MSH biosynthesis and recycling (mshC, mca, mtr) (Ehira et al., 2009;Busche et al., 2012). The regulation of the Trx and MSH pathways by σ R /RsrA or σ H /RshA is conserved among Actinomycetes (Park and Roe, 2008;Antelmann and Helmann, 2011;Kim et al., 2012). Thus, it is common in Gram-positive bacteria that the genes for BSH and MSH biosynthesis pathways are under redox-control of the major disulfide stress regulators, Spx in Firmicutes bacteria and RsrA/RshA in Actinomycetes, respectively.

Functions of Mycothiol and MSH-Dependent Enzymes in Actinomycetes
MSH serves as the major thiol-redox buffer in Actinomycetes. MSH is oxidized to MSH disulfide (MSSM) under oxidative stress conditions. The mycothiol disulfide reductase Mtr maintains MSH in its reduced state at the expense of NADPH. MSH is involved in protection against oxidative and electrophile stress, alkylating agents, toxins, antibiotics (erythromycin, vancomycin, rifampin, azithromycin), heavy metal stress, aromatic compounds, ethanol and glyphosate in Streptomycetes, Mycobacteria and Corynebacteria (Buchmeier et al., , 2006Rawat et al., 2007;Liu et al., 2013) (Table 1). MSH is used as a cofactor for MSH-dependent enzymes during detoxification of toxins, electrophiles and antibiotics in Actinomycetes (Figure 9, Table 1). MSH forms conjugates with xenobiotics and antibiotics either spontaneously or by the DinBfamily MSH S-transferases . The MSH S-transferase Mst of M. smegmatis was shown to catalyze the conjugation of monochlorobimane and DTNB to MSH but its natural substrate is not known . MSH-Sconjugates are rapidly cleaved by the MSH-S-conjugate amidase (Mca) to glucoseamine-myo-inositol (GlcN-Ins) and mercapturic acid derivatives (AcCysSR) that are excreted from the cell. Mca is the major detoxification enzyme for MSH S-conjugates with antibiotics, including cerulenin and rifamycin in Mycobacteria . Interestingly, MSH and the Mca-homologs LmbT, LmbV and LmbE play also a direct role in the biosynthesis of the sulfur-containing lincosamide antibiotic lincomycin in Streptomyces lincolnensis (Zhao et al., 2015). MSH functions as the sulfur donor for incorporation of the methylmercapto group into lincomycin after thiol exchange. In addition, ergothioneine (EGT), that is utilized as another thiol by Actinomycetes, acts as a carrier for the assembly of the N-methylated 4-propyl-L-proline (PPL) and lincosamide moieties to form lincomycin. EGT and MSH were shown to function in lincomycin biosynthesis through unusual S-glycosylations documenting a first biochemical role of LMW thiols in bacteria. Since the biosynthetic pathways for many sulfur-containing natural compounds include Mca homologs, the involvement of LMW thiols in natural product biosynthesis might be a common mechanism (Zhao et al., 2015).
MSH functions as a cofactor for many redox enzymes that are involved in the detoxification of peroxides, electrophiles (formaldehyde), NO, aromatic compounds (maleylpyruvate) and arsenate (Fahey, 2013) (Table 1). There is evidence for a MSHperoxidase Mpx involved in peroxide detoxification that was identified as S-mycothiolated Gpx-homolog under oxidative stress in C. glutamicum . The MSH-dependent alcohol dehydrogenase MscR (MSNO reductase/formaldehyde dehydrogenase) catalyzes the detoxification of formaldehyde and S-nitrosyl-mycothiol (MSNO) . MSH reacts with formaldehyde to MS-CH 2 OH that is converted to formate by MscR. MscR also converts MSNO to MSH sulfinamide (MSONH 2 ). In C. glutamicum, a similar MSH-dependent pathway for formaldehyde oxidation by the MSH-dependent formaldehyde dehydrogenase AdhE/FadH has been characterized (Lessmeier et al., 2013;Witthoff et al., 2013). In C. glutamicum, MSH is further involved in degradation of aromatic compounds, including gentisate, 3-hydroxybenzoate, maleylpyruvate, resorcinol, and naphthalene and msh mutants were unable to grow on these substrates . MSH functions as a cofactor for the maleylpyruvate isomerase in the gentisate ringcleavage pathway to catalyze the isomerization of maleylpyruvate to fumaryl pyruvate in C. glutamicum (Feng et al., 2006). Similarly, MSH was suggested as a cofactor for enzymes of the naphthalene and resorcinol degradation pathway .
MSH enhanced also the robustness of C. glutamicum during industrial production of glutamate and L-lysine (Liu et al., 2014). The overexpression of mshA resulted in increased MSH biosynthesis and higher resistance of C. glutamicum to peroxides, methylglyoxal, antibiotics (erythromycin and streptomycin), metal ions, organic acids, furfural and ethanol (Liu et al., 2014). Thus, the increased biosynthesis of LMW thiol redox buffers, as shown for GSH in C. acetobutylicum and MSH in  (Zhao et al., 2015). MSH serves as a cofactor for the alcohol dehydrogenase AdhE/MscR in Mycobacteria and Corynebacteria for detoxification of formaldehyde to formate and MSNO to MSO2H. MSH functions in detoxification of maleylpyruvate as a cofactor for maleylpyruvate isomerase in C. glutamicum. Arsenate reductases CgArsC1 and CgArsC2 conjugate MSH and arsenate As(V) to form As(V)-SM that is reduced to As(III) by Mrx1. In M. tuberculosis, MSH is important under infection conditions and for growth and survival. Under conditions of NaOCl stress, proteins are oxidized to mixed disulfides with MSH, termed as S-mycothiolations which is reversed by mycoredoxins.
C. glutamicum, might be a promising strategy to engineer robust industrial production strains.
In Mycobacterium tuberculosis, MSH is essential for growth and survival of M. tuberculosis under infection conditions (Sareen et al., 2003;Sassetti and Rubin, 2003). In addition, MSH is required to activate the antituberculosis prodrug isoniazid and hence M. tuberculosis mshA mutants are resistant to isoniazid . Tuberculosis (TB) causes still nearly 2 million death each year and multiple and extensive drug resistant strains occur that require new targets for antituberculosis drugs. Thus, inhibitors of MSH biosynthesis enzymes are promising candidates for antituberculosis drug developments. Several MSH biosynthesis inhibitors have been applied that target the MSH-S-conjugate amidase Mca, the deacetylase MshB, the cysteine ligase MshC and the MSSM reductase Mtr that are attractive antituberculosis drug targets (Nilewar and Kathiravan, 2014).
the most abundantly S-mycothiolated proteins in NaOCl-treated cells . S-mycothiolation of MalP is important for oxidative stress resistance in C. glutamicum since the malP deletion mutant is NaOCl-sensitive in growth assays. MalP functions in glycogen degradation during the stationary phase. Smycothiolation of MalP may prevent glycogen degradation under NaOCl stress since the glycogen content remained stable despite a strongly decreased glucose uptake rate.
The mycoredoxin-1 (Mrx1) has been characterized as glutaredoxin-homolog of Actinomycetes in reduction of MSH mixed disulfides (Van Laer et al., 2012) (Figure 8). Mrx-1 has a typical Trx-like fold with a CGYC motif and a cis-Pro57 in a groove that presumable binds MSH. The redox potential of Mrx-1 was calculated as E 0 ′ = −218 mV and the pK a of the active site Cys17 was 5.1-5.6. Mrx-1 catalyzed de-mycothiolation in a hydroxyethyl disulfide (HED) assay and is coupled to the MSH/Mtr/NADPH pathway. Mrx-1 operates via a monothiol reaction mechanism in the de-mycothiolation reaction analogous to most glutaredoxins that are involved in de-glutathionylation. The first Mrx1 substrate was identified as the thiol-peroxidase Tpx that was S-mycothiolated at its active site Cys60 and resolving site Cys94 in C. glutamicum in vivo under hypochlorite stress . Tpx showed NADPH-linked peroxidase activity and reduced H 2 O 2 in a Trx/TrxR-coupled electron assay. Smycothiolation of Tpx inhibits the peroxidase activity which was restored after reduction by the Mrx1/MSH/Mtr pathway. Thus, S-mycothiolation controls Tpx activity and protects the peroxidatic Cys against overoxidation. In M. tuberculosis, Mrx1 has been shown to reduce the one-Cys peroxiredoxin AhpE (Hugo et al., 2014). AhpE is a membrane-associated peroxidase that detoxifies peroxinitrite and fatty acid hydroperoxides as preferred substrates (Hugo et al., 2009;Reyes et al., 2011). AhpE is oxidized by peroxides to form a sulfenic acid intermediate (AhpE-SOH) that can be reduced directly by Mrx1. Alternatively, AhpE-SOH can react with MSH to S-mycothiolated AhpE-SSM which is reduced by the Mrx1/MSH/Mtr electron pathway (Hugo et al., 2014). The direct AhpE-SOH reduction may occur in the membrane when MSH is not available and the formation of AhpE-SSM and subsequent Mrx1-reduction was suggested to predominate in the cytosol. Interestingly, the reducing mechanism of AhpE-SSM is similar to the detoxification of arsenate by CgArsC1 and CgArsC2. Arsenate reacts with MSH to an arseno-(V)-MSH complex that is reduced by Mrx1 releasing As(III) and Mrx1-SSM that is recycled by the MSH/Mtr/NADPH electron pathway (Ordonez et al., 2009;Villadangos et al., 2011). It remains to be shown if AhpE is mycothiolated under oxidative stress in M. tuberculosis cells in vivo. These results show that Mrx1 functions as glutaredoxin homolog in C. glutamicum and M. tuberculosis in the reduction of S-mycothiolated peroxiredoxins (Tpx and AhpE), when coupled to the MSH/Mtr/NADPH electron pathway and as electron donor for arsenate reductase in arsenate detoxification.
Recently, Mrx1 has been coupled to redox sensitive GFP (roGFP2) to construct a new genetically encoded biosensor for dynamic measurements of the MSH redox potential in different M. tuberculosis strains (Bhaskar et al., 2014). This study revealed phenotypic redox heterogeneity of E 0 ′ (MSSM/MSH) within Mycobacteria inside infected macrophages that are caused by sub-vacuolar compartments. Those sub-populations with higher E 0 ′ (MSSM/MSH) were more susceptible to clinical relevant antibiotics whereas populations with lower MSH redox potentials were resistant to antibiotics. The results further show that several anti-TB drugs induce oxidative stress in M. tuberculosis during infections. In conclusion, this Mrx1-roGFP2 biosensor is a promising tool to study MSH redox potential changes of M. tuberculosis under infections and antibiotic treatments. This is the first example for a genetically encoded redox biosensor that measures dynamic changes of the mycothiol redox potential in bacteria. Future studies should be directed to apply similar biosensors in other pathogenic bacteria to study the dynamics of redox potential changes during infections.

Conclusion and Perspectives for Future Research
In this review, we provide an overview about the biosynthesis pathways and functions of the bacterial redox buffers glutathione, bacillithiol and mycothiol and their regulatory roles for protein S-thiolations. Bacterial redox buffers maintain the reduced state of the cytoplasm and function as cofactors of conserved enzymes for detoxification of ROS, RES, chlorines, antibiotics and xenobiotics. These thiol-dependent enzymes include NADPH-dependent disulfide reductases (Gor, Mtr, YpdA) and related glutaredoxin-like enzymes (Grx, Mrx, Brx), DinBfamily S-transferases (Gst, Mst, BstA), S-conjugate amidases (Mca, Bca) and glyoxalases (GloAB, GlxAB). However, some detoxification enzymes still need to be characterized in BSHutilizing bacteria, including the BSH-dependent formaldehyde reductase (AdhA), the putative BSH peroxidase (Bpx) or thioldependent dioxygenases (MhqA, MhqE and MhqO) (Antelmann et al., 2008). The discovery of the biochemical functions of MSH, EGT and S-transferases in the lincomycin antibiotic biosynthesis opens perspectives to characterize the roles of thiol-redox buffers in the biosynthesis of sulfur-containing co-factors, natural compounds and antibiotics in other bacteria.
The structures of BSH and MSH are similar and the BSH biosynthesis enzymes BshA, BshB and BshC are homologous to the MSH biosynthesis enzymes MshA, MshB, and MshC. However, the crystal structure of BshC has revealed significant differences compared to MshC which requires further studies to understand the still unknown cysteine ligation mechanism of BshC (Vanduinen et al., 2015). It is further interesting, that the levels of BSH and MSH vary strongly between Firmicutes and Actinomycetes and also during growth and stress conditions. While Mycobacteria produce up to 20 mM MSH, the levels of BSH are much lower reaching 1-6 mM in Firmicutes bacteria. The differences in BSH and MSH levels during growth and under stress can be explained by the redox control of the BSH and MSH biosynthesis enzymes by the major thiol-based redox sensors (Spx and RsrA/RshA), presumably to enhance the redox buffer capacity under certain conditions to keep the reduced state of the cytoplasm. In contrast, redox regulation of GSH biosynthesis genes has not been shown. However, the pathogen L. monocytogenes is able to synthesize GSH and to import hostderived GSH as adaptation strategy under infection conditions (Reniere et al., 2015). Importantly, synthesized and host-derived GSH both contribute to virulence factor regulation in L. monocytogenes, while GSH-import was required for full virulence in S. pneumoniae (Potter et al., 2012;Reniere et al., 2015). Overall, the roles of GSH, BSH and MSH for virulence and pathogen fitness have been shown for many important human pathogens, including L. monocytogenes, S. pneumoniae, S. Typhimurium, S. aureus, and M. tuberculosis. Future studies in the field of infection biology should be directed to understand the molecular mechanisms of virulence factor regulation by thiol-redox buffers that might involve also protein S-thiolation mechanisms. The GSH, BSH and MSH biosynthesis enzymes, GSH uptake systems as well as S-thiolated proteins could be promising drug targets for the development of novel anti-infectives against emerging drug resistant strains of S. pneumoniae, S. aureus and M. tuberculosis. Thus, the large scale identification and quantification of Sthiolated proteins in pathogens is an important topic for future research.
Advances in mass spectrometry and chemical probe design have facilitated the development of more sensitive redox proteomics methods, such as the NEM-biotin switch assay or the Gsp-biotin assay to study targets for protein S-glutathionylation at a global scale (Lind et al., 2002;Kehr et al., 2011;Lin et al., 2015). In addition, numerous BSH-and MSH-mixed protein disulfides have been identified recently under disulfide stress conditions, such as NaOCl and diamide. However, more quantitative MS-based redox proteomics approaches are required to determine the level of mixed BSH-and MSH-protein disulfides by combining the direct shotgun approach with OxICAT or the NEM-biotin switch assay coupled to Brx or Mrx1 (Leichert et al., 2008;Kehr et al., 2011). In addition, the regulatory roles for only few S-bacillithiolated and S-mycothiolated proteins have been studied thus far, including the redox regulator OhrR and the methionine synthase MetE (Lee et al., 2007;Chi et al., 2011). However, many interesting metabolic enzymes, redox-sensing transcription factors or virulence factors might be controlled by protein S-thiolations in the pathogenic bacteria S. aureus and M. tuberculosis that remain to be elucidated in future research. Thus, it is an exciting field for new frontiers of science to unravel the regulatory potential of emerging protein S-thiolations in bacteria.