AUTHOR=Matsunaga Satoko , Masaoka Takashi , Sawasaki Tatsuya , Morishita Ryo , Iwatani Yasumasa , Tatsumi Masashi , Endo Yaeta , Yamamoto Naoki , Sugiura Wataru , Ryo Akihide TITLE=A cell-free enzymatic activity assay for the evaluation of HIV-1 drug resistance to protease inhibitors JOURNAL=Frontiers in Microbiology VOLUME=6 YEAR=2015 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.01220 DOI=10.3389/fmicb.2015.01220 ISSN=1664-302X ABSTRACT=
Due to their high frequency of genomic mutations, human retroviruses often develop resistance to antiretroviral drugs. The emergence of drug-resistant human immunodeficiency virus type 1 (HIV-1) is a significant obstacle to the effective long-term treatment of HIV infection. The development of a rapid and versatile drug-susceptibility assay would enable acquisition of phenotypic information and facilitate determination of the appropriate choice of antiretroviral agents. In this study, we developed a novel