AUTHOR=Szczepaniak Joanna , Łukaszewicz Marcin , Krasowska Anna 

TITLE=Estimation of Candida albicans ABC Transporter Behavior in Real-Time via Fluorescence

JOURNAL=Frontiers in Microbiology

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2015.01382

DOI=10.3389/fmicb.2015.01382

ISSN=1664-302X

ABSTRACT=<p>We present a fluorometric method for determining ABC transporter activity in the pathogenic fungus <italic>C. albicans</italic> during different growth phases and in response to glucose. The carbocyanine dye diS-C<sub>3</sub>(3) was previously used to monitor plasma membrane potentials and test the influence of surface-active compounds in membrane polarization. We used diS-C<sub>3</sub>(3) to show changes in fluorescence kinetics that reflect changes in the activity of ABC transporters in <italic>C. albicans</italic> growth. Cdr1-GFP fluorescence, revealed that Cdr1p relocates to the inside of the cell after the early-log growth phase. Addition of glucose to the cell suspension resulted in Cdr1p transporter expression in the <italic>CDR2</italic>-knockout strain. We confirmed the diS-C<sub>3</sub>(3) results by standard RT-PCR and Western blotting.</p>