AUTHOR=Kubota Takaaki , Kobayashi Tohru , Nunoura Takuro , Maruyama Fumito , Deguchi Shigeru 

TITLE=Enantioselective Utilization of D-Amino Acids by Deep-Sea Microorganisms

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.00511

DOI=10.3389/fmicb.2016.00511

ISSN=1664-302X

ABSTRACT=<p>Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments. The isolates were phylogenetically assigned to Alphaproteobacteria, Gammmaproteobacteria, and Bacilli. Some of the isolates exhibited high enantioselective degradation activities to various DAAs. In particular, the Alphaproteobacteria <italic>Nautella</italic> sp. strain A04V exhibited robust growth in minimal medium supplemented with D-Val as a sole carbon and nitrogen source, whereas its growth was poor on minimal medium supplemented with L-Val instead of D-Val. Its growth was facilitated most when racemic mixtures of valine were used. In contrast, the <italic>Nautella</italic> strains isolated from shallow-sea grew only with L-Val. No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.</p>