%A Khaw,Yam Sim %A Chan,Yoke Fun %A Jafar,Faizatul Lela %A Othman,Norlijah %A Chee,Hui Yee %D 2016 %J Frontiers in Microbiology %C %F %G English %K Malaysia,complete genome,HRV-C,Genomic feature,phylogenetic,negative selection %Q %R 10.3389/fmicb.2016.00543 %W %L %M %P %7 %8 2016-April-29 %9 Original Research %+ Hui Yee Chee,Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia,Serdang, Malaysia,cheehy@upm.edu.my %# %! Study of HRV-C complete genomes from Malaysia %* %< %T Comparative Genetic Analyses of Human Rhinovirus C (HRV-C) Complete Genome from Malaysia %U https://www.frontiersin.org/articles/10.3389/fmicb.2016.00543 %V 7 %0 JOURNAL ARTICLE %@ 1664-302X %X Human rhinovirus-C (HRV-C) has been implicated in more severe illnesses than HRV-A and HRV-B, however, the limited number of HRV-C complete genomes (complete 5′ and 3′ non-coding region and open reading frame sequences) has hindered the in-depth genetic study of this virus. This study aimed to sequence seven complete HRV-C genomes from Malaysia and compare their genetic characteristics with the 18 published HRV-Cs. Seven Malaysian HRV-C complete genomes were obtained with newly redesigned primers. The seven genomes were classified as HRV-C6, C12, C22, C23, C26, C42, and pat16 based on the VP4/VP2 and VP1 pairwise distance threshold classification. Five of the seven Malaysian isolates, namely, 3430-MY-10/C22, 8713-MY-10/C23, 8097-MY-11/C26, 1570-MY-10/C42, and 7383-MY-10/pat16 are the first newly sequenced complete HRV-C genomes. All seven Malaysian isolates genomes displayed nucleotide similarity of 63–81% among themselves and 63–96% with other HRV-Cs. Malaysian HRV-Cs had similar putative immunogenic sites, putative receptor utilization and potential antiviral sites as other HRV-Cs. The genomic features of Malaysian isolates were similar to those of other HRV-Cs. Negative selections were frequently detected in HRV-Cs complete coding sequences indicating that these sequences were under functional constraint. The present study showed that HRV-Cs from Malaysia have diverse genetic sequences but share conserved genomic features with other HRV-Cs. This genetic information could provide further aid in the understanding of HRV-C infection.