TY - JOUR AU - Long, Jing AU - Basu Roy, Robindra AU - Zhang, Yanjia J. AU - Antrobus, Robin AU - Du, Yuxian AU - Smith, Duncan L. AU - Weekes, Michael P. AU - Javid, Babak PY - 2016 M3 - Original Research TI - Plasma Membrane Profiling Reveals Upregulation of ABCA1 by Infected Macrophages Leading to Restriction of Mycobacterial Growth JO - Frontiers in Microbiology UR - https://www.frontiersin.org/articles/10.3389/fmicb.2016.01086 VL - 7 SN - 1664-302X N2 - The plasma membrane represents a critical interface between the internal and extracellular environments, and harbors multiple proteins key receptors and transporters that play important roles in restriction of intracellular infection. We applied plasma membrane profiling, a technique that combines quantitative mass spectrometry with selective cell surface aminooxy-biotinylation, to Bacille Calmette–Guérin (BCG)-infected THP-1 macrophages. We quantified 559 PM proteins in BCG-infected THP-1 cells. One significantly upregulated cell-surface protein was the cholesterol transporter ABCA1. We showed that ABCA1 was upregulated on the macrophage cell-surface following infection with pathogenic mycobacteria and knockdown of ABCA1 resulted in increased mycobacterial survival within macrophages, suggesting that it may be a novel mycobacterial host-restriction factor. ER -