AUTHOR=Uytterhoeven Birgit , Lathouwers Thomas , Voet Marleen , Michiels Chris W. , Lavigne Rob 

TITLE=A Protein Interaction Map of the Kalimantacin Biosynthesis Assembly Line

JOURNAL=Frontiers in Microbiology

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2016.01726

DOI=10.3389/fmicb.2016.01726

ISSN=1664-302X

ABSTRACT=<p>The antimicrobial secondary metabolite kalimantacin (also called batumin) is produced by a hybrid polyketide/non-ribosomal peptide system in <italic>Pseudomonas fluorescens</italic> BCCM_ID9359. In this study, the kalimantacin biosynthesis gene cluster is analyzed by yeast two-hybrid analysis, creating a protein–protein interaction map of the entire assembly line. In total, 28 potential interactions were identified, of which 13 could be confirmed further. These interactions include the dimerization of ketosynthase domains, a link between assembly line modules 9 and 10, and a specific interaction between the <italic>trans</italic>-acting enoyl reductase BatK and the carrier proteins of modules 8 and 10. These interactions reveal fundamental insight into the biosynthesis of secondary metabolites. This study is the first to reveal interactions in a complete biosynthetic pathway. Similar future studies could build a strong basis for engineering strategies in such clusters.</p>