AUTHOR=Guo Jiubiao , Chan Edward W. C. , Chen Sheng , Zeng Zhenling 

TITLE=Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of Salmonella

JOURNAL=Frontiers in Microbiology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.00008

DOI=10.3389/fmicb.2017.00008

ISSN=1664-302X

ABSTRACT=<p>A novel, rapid and simple fluorescence resonance energy transfer (FRET) based <italic>Salmonella</italic> specific gene, <italic>invA</italic>, detection system was developed, in which quantum dots (QDs) and graphene oxide (GO) worked as fluorescent donor and quencher, respectively. By measuring the fluorescence intensity signal, the <italic>Salmonella</italic> specific <italic>invA</italic> gene could be sensitively and specifically detected with a limit of detection (LOD) of ∼4 nM of the <italic>invA</italic> gene in 20 min. The developed system has the potential to be used for <italic>Salmonella</italic> detection in food and environmental samples and further developed into a platform for detection of other bacterial pathogens.</p>